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Protein

Polymerase basic protein 2

Gene

PB2

Organism
Influenza A virus (strain A/Swine/Wisconsin/1/1967 H1N1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Plays an essential role in transcription initiation and cap-stealing mechanism, in which cellular capped pre-mRNAs are used to generate primers for viral transcription. Recognizes and binds the 7-methylguanosine-containing cap of the target pre-RNA which is subsequently cleaved after 10-13 nucleotides by the viral protein PA. Plays a role in the initiation of the viral genome replication and modulates the activity of the ribonucleoprotein (RNP) complex. In addition, participates in the inhibition of type I interferon induction through interaction with and inhibition of the host mitochondrial antiviral signaling protein MAVS.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei627Mammalian adaptationUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processCap snatching, Eukaryotic host gene expression shutoff by virus, Eukaryotic host transcription shutoff by virus, Host gene expression shutoff by virus, Host-virus interaction, Inhibition of host innate immune response by virus, Inhibition of host MAVS by virus, Inhibition of host RLR pathway by virus, Inhibition of host RNA polymerase II by virus, mRNA capping, mRNA processing, Viral immunoevasion, Viral transcription

Names & Taxonomyi

Protein namesi
Recommended name:
Polymerase basic protein 2UniRule annotation
Alternative name(s):
RNA-directed RNA polymerase subunit P3UniRule annotation
Gene namesi
Name:PB2UniRule annotation
OrganismiInfluenza A virus (strain A/Swine/Wisconsin/1/1967 H1N1)
Taxonomic identifieri382855 [NCBI]
Taxonomic lineageiVirusesssRNA virusesssRNA negative-strand virusesOrthomyxoviridaeInfluenzavirus A
Virus hostiAves [TaxID: 8782]
Homo sapiens (Human) [TaxID: 9606]
Sus scrofa (Pig) [TaxID: 9823]
Proteomesi
  • UP000116872 Componenti: Genome

Subcellular locationi

  • Virion UniRule annotation
  • Host nucleus UniRule annotation
  • Host mitochondrion UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Host mitochondrion, Host nucleus, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003730381 – 759Polymerase basic protein 2Add BLAST759

Interactioni

Subunit structurei

Influenza RNA polymerase is composed of three subunits: PB1, PB2 and PA. Interacts (via N-terminus) with PB1 (via C-terminus). Interacts with nucleoprotein NP (via N-terminus). Interacts (via N-terminus) with host MAVS (via N-terminus); this interaction inhibits host innate immune response.UniRule annotation

Structurei

3D structure databases

SMRiA8C8X3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi736 – 739Nuclear localization signalUniRule annotation4

Sequence similaritiesi

Belongs to the influenza viruses PB2 family.UniRule annotation

Family and domain databases

HAMAPiMF_04062. INV_PB2. 1 hit.
InterProiView protein in InterPro
IPR001591. RNA_pol_PB2_orthomyxovir.
PfamiView protein in Pfam
PF00604. Flu_PB2. 1 hit.
ProDomiView protein in ProDom or Entries sharing at least one domain
PD001667. RNA_pol_PB2_orthomyxovir. 1 hit.

Sequencei

Sequence statusi: Complete.

A8C8X3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MERIKELRDL MSQSRTREIL TRTTVDHMAI IKKYTSGRQE KNPALRMKWM
60 70 80 90 100
MAMKYPITAD KRIIETIPER NEQGQTLWSR TSDAGSDRVM VSPLAVTWWN
110 120 130 140 150
RNGPTASTVH YPKVYRTYFE KVERLKHGTF GPVHFRNHVK IRRRVDINPG
160 170 180 190 200
HADLSAKEAQ DVIMEVVFPN EVGARILTSE SQLMITKEKK EELQECKISP
210 220 230 240 250
LMVAYMLERE LVRKTRFLPV AGGTSSVYIE VLHLTQGACW EQLYTPGGEV
260 270 280 290 300
RNDDVGQSLI IAARSIVRRA TVSADPLASL LEMCHSTQIG GVRMVDILRQ
310 320 330 340 350
NPTEEQAVDI CKAAMGLRIS SSFSFGGFTF KRTSGSSTKK EEEVLTGNLQ
360 370 380 390 400
TLKIRVHEGY EEFTMVGKRA TAILRKATRR LVQLIVSGRD EQSIAEAIIV
410 420 430 440 450
AMVFSQEDCM IKAVRGDLNF VNRANQRLNP MHQLLRHFQK DAKILFQNWG
460 470 480 490 500
IEPIDNVMGM IGVLPDLTPS TEMSMRGVRI SKMGVDEYSS TERVVVSIDR
510 520 530 540 550
FLRVRDQQGN VLLSPEEVSE TQGTEKLTIT YSSSMMWEVN GPESVLVNTY
560 570 580 590 600
QWIIRNWETV KIQWSQDPTM LYNKMEFEPF QSLVPKAARG QYSGFVRTLF
610 620 630 640 650
QQMRDVLGTF DTVQIIKLLP FAAAPPKQSR MQFSSLTVNV RGSGMRILIR
660 670 680 690 700
GNSPVFNYNK GTKRLTVLGK DAGALNEDPD EGTTGVESAV LRGFLILGRE
710 720 730 740 750
DRRYGPALSI NELSSLAKGE KANVLIGQGD VVLVMKRKRD SSILTDSQTA

TKRIRMAIN
Length:759
Mass (Da):85,784
Last modified:November 13, 2007 - v1
Checksum:iAB3C033E34E3ABFB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CY026298 Viral cRNA. Translation: ABV82594.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CY026298 Viral cRNA. Translation: ABV82594.1.

3D structure databases

SMRiA8C8X3.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

HAMAPiMF_04062. INV_PB2. 1 hit.
InterProiView protein in InterPro
IPR001591. RNA_pol_PB2_orthomyxovir.
PfamiView protein in Pfam
PF00604. Flu_PB2. 1 hit.
ProDomiView protein in ProDom or Entries sharing at least one domain
PD001667. RNA_pol_PB2_orthomyxovir. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPB2_I67A2
AccessioniPrimary (citable) accession number: A8C8X3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: November 13, 2007
Last modified: May 10, 2017
This is version 41 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.