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A8C8K1

- PA_I07A0

UniProt

A8C8K1 - PA_I07A0

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Protein

Polymerase acidic protein

Gene

PA

Organism
Influenza A virus (strain A/USA:Texas/UR06-0195/2007 H1N1)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Plays an essential role in viral RNA transcription and replication by forming the heterotrimeric polymerase complex together with PB1 and PB2 subunits. The complex transcribes viral mRNAs by using a unique mechanism called cap-snatching. It consists in the hijacking and cleavage of host capped pre-mRNAs. These short capped RNAs are then used as primers for viral mRNAs. The PB2 subunit is responsible for the binding of the 5' cap of cellular pre-mRNAs which are subsequently cleaved after 10-13 nucleotides by the PA subunit that carries the endonuclease activity. In addition of its function in viral transcription, PA also plays an essential role in viral RNA synthesis and promotes the formation of the trimeric polymerase complex.By similarity

GO - Molecular functioni

  1. RNA binding Source: InterPro
  2. RNA-directed RNA polymerase activity Source: InterPro

GO - Biological processi

  1. suppression by virus of host RNA polymerase II activity Source: UniProtKB-KW
  2. transcription, DNA-templated Source: InterPro
Complete GO annotation...

Keywords - Biological processi

Cap snatching, Eukaryotic host gene expression shutoff by virus, Eukaryotic host transcription shutoff by virus, Host gene expression shutoff by virus, Host-virus interaction, Inhibition of host RNA polymerase II by virus

Names & Taxonomyi

Protein namesi
Recommended name:
Polymerase acidic protein
Alternative name(s):
RNA-directed RNA polymerase subunit P2
Gene namesi
Name:PA
OrganismiInfluenza A virus (strain A/USA:Texas/UR06-0195/2007 H1N1)
Taxonomic identifieri455880 [NCBI]
Taxonomic lineageiVirusesssRNA negative-strand virusesOrthomyxoviridaeInfluenzavirus A
Virus hostiAves [TaxID: 8782]
Homo sapiens (Human) [TaxID: 9606]
Sus scrofa (Pig) [TaxID: 9823]

Subcellular locationi

Host cytoplasm. Host nucleus
Note: PB1 and PA are transported in the host nucleus as a complex.By similarity

Keywords - Cellular componenti

Host cytoplasm, Host nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 716716Polymerase acidic proteinPRO_0000372995Add
BLAST

Post-translational modificationi

Phosphorylated on serines and threonines by host kinases, including human casein kinase II.By similarity

Keywords - PTMi

Phosphoprotein

Interactioni

Subunit structurei

Influenza RNA polymerase is composed of three subunits: PB1, PB2 and PA. Interacts (via C-terminus) with PB1 (via N-terminus).By similarity

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni124 – 13916Nuclear localization signal 1 (NLS1)By similarityAdd
BLAST
Regioni184 – 24764Nuclear localization signal 2 (NLS2)By similarityAdd
BLAST

Sequence similaritiesi

Belongs to the influenza viruses PA family.Curated

Family and domain databases

InterProiIPR001009. RNA-dir_pol_influenzavirus.
[Graphical view]
PfamiPF00603. Flu_PA. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by ribosomal frameshifting. Align

Isoform PA (identifier: A8C8K1-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEDFVRQCFN PMIVELAEKA MKEYGEDLKI ETNKFAAICT HLEVCFMYSD
60 70 80 90 100
FHFINEQGES IIVEPEDPNA LLKHRFEIIE GRDRTMAWTV VNSICNTTGA
110 120 130 140 150
EKPKFLPDLY DYKENRFIEI GVTRREVHIY YLEKANKIKS EKTHIHIFSF
160 170 180 190 200
TGEEMATKAD YTLDEESRAR IKTRLFTIRQ EMASRGLWDS FRQSERGEET
210 220 230 240 250
IEERFEITGT MRRLADQSLP PNFSCIENFR AYVDGFEPNG YIEGKLSQMS
260 270 280 290 300
KEVNARIEPF LKTTPRPIRL PNGPPCFQRS KFLLMDSLKL SIEDPNHEGE
310 320 330 340 350
GIPLYDAIKC MRTFFGWKEP TVVKPHEKGI NPNYLLSWKQ VLEELQDIES
360 370 380 390 400
EEKIPRTKNM KKTSQLKWAL GENMAPEKVD FDDCKDVSDL KQYDSDEPEL
410 420 430 440 450
RSFSSWIQNE FNKACELTDS IWIELDEIGE DVAPIEHIAS MRRNYFTAEV
460 470 480 490 500
SHCRATEYIM KGVYINTALL NASCAAMDDF QLIPMISKCR TKEGRRKTNL
510 520 530 540 550
YGFIIKGRSH LRNDTDVVNF VSMEFSLTDP RLEPHKWERY CVLEIGDMLL
560 570 580 590 600
RSAIGQVSRP MFLYVRTNGT SKIKMKWGME MRRCLLQSLQ QIESMIEAES
610 620 630 640 650
SVKEKDMTKE FFENRSETWP IGESPEGVEE GSIGKVCRTL LAKSVFNSLY
660 670 680 690 700
ASPQLEGFSA ESRKLLLIVQ ALRDNLEPGT FDLGGLYEAI EECLINDPWV
710
LLNASWFNSF LTHALR
Length:716
Mass (Da):82,994
Last modified:November 13, 2007 - v1
Checksum:i6B8B6B4597C358BA
GO
Isoform PA-X (identifier: P0DJW6-1) [UniParc]FASTAAdd to Basket

The sequence of this isoform can be found in the external entry P0DJW6.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:252
Mass (Da):29,339
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CY026216 Viral cRNA. Translation: ABV45933.1.

Keywords - Coding sequence diversityi

Ribosomal frameshifting

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CY026216 Viral cRNA. Translation: ABV45933.1 .

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR001009. RNA-dir_pol_influenzavirus.
[Graphical view ]
Pfami PF00603. Flu_PA. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. The NIAID Influenza Genome Sequencing Consortium
    Submitted (SEP-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Entry informationi

Entry nameiPA_I07A0
AccessioniPrimary (citable) accession number: A8C8K1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: November 13, 2007
Last modified: October 29, 2014
This is version 26 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3