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Protein

Endoribonuclease Dicer-like

Gene

GL50803_103887

Organism
Giardia intestinalis (strain ATCC 50803 / WB clone C6) (Giardia lamblia)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in cleaving double-stranded RNA in the RNA interference (RNAi) pathway. It produces 21 to 23 bp dsRNAs (siRNAs) which target the selective destruction of homologous RNAs.1 Publication

Cofactori

Mg2+1 Publication, Mn2+1 PublicationNote: Binds 2 magnesium or manganese ions per subunit.2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi336 – 3361Magnesium or manganese 1Combined sources2 Publications
Metal bindingi404 – 4041Magnesium or manganese 1Combined sources2 Publications
Metal bindingi407 – 4071Magnesium or manganese 1Combined sources2 Publications
Metal bindingi649 – 6491Magnesium or manganese 2Combined sources2 Publications
Metal bindingi720 – 7201Magnesium or manganese 2Combined sources2 Publications
Metal bindingi723 – 7231Magnesium or manganese 2Combined sources2 Publications

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

RNA-mediated gene silencing

Keywords - Ligandi

Magnesium, Manganese, Metal-binding, Nucleotide-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Endoribonuclease Dicer-like (EC:3.1.26.-1 Publication)
Gene namesi
ORF Names:GL50803_103887
OrganismiGiardia intestinalis (strain ATCC 50803 / WB clone C6) (Giardia lamblia)
Taxonomic identifieri184922 [NCBI]
Taxonomic lineageiEukaryotaDiplomonadidaHexamitidaeGiardiinaeGiardia
Proteomesi
  • UP000001548 Componenti: Unassembled WGS sequence

Organism-specific databases

EuPathDBiGiardiaDB:GL50803_103887.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 754754Endoribonuclease Dicer-likePRO_0000371303Add
BLAST

Interactioni

Subunit structurei

Homodimer.2 Publications

Protein-protein interaction databases

STRINGi184922.XP_001705536.1.

Structurei

Secondary structure

1
754
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi4 – 118Combined sources
Beta strandi16 – 2510Combined sources
Beta strandi33 – 353Combined sources
Beta strandi39 – 413Combined sources
Beta strandi44 – 474Combined sources
Helixi58 – 7518Combined sources
Helixi83 – 886Combined sources
Helixi92 – 943Combined sources
Beta strandi95 – 973Combined sources
Beta strandi110 – 1178Combined sources
Helixi125 – 13511Combined sources
Helixi140 – 1423Combined sources
Helixi144 – 1463Combined sources
Turni151 – 1533Combined sources
Beta strandi158 – 1603Combined sources
Turni162 – 1665Combined sources
Beta strandi168 – 17912Combined sources
Beta strandi182 – 1865Combined sources
Beta strandi189 – 1924Combined sources
Helixi193 – 1997Combined sources
Beta strandi210 – 2178Combined sources
Turni218 – 2236Combined sources
Beta strandi240 – 2423Combined sources
Turni244 – 2463Combined sources
Helixi258 – 2625Combined sources
Helixi265 – 28218Combined sources
Helixi283 – 2853Combined sources
Helixi293 – 30412Combined sources
Beta strandi313 – 3153Combined sources
Beta strandi318 – 3203Combined sources
Helixi323 – 3253Combined sources
Helixi328 – 35427Combined sources
Helixi360 – 37112Combined sources
Helixi373 – 38210Combined sources
Turni386 – 3883Combined sources
Helixi402 – 41514Combined sources
Helixi419 – 43113Combined sources
Helixi434 – 4363Combined sources
Helixi446 – 45611Combined sources
Helixi462 – 47110Combined sources
Turni472 – 4765Combined sources
Beta strandi480 – 4834Combined sources
Helixi484 – 49512Combined sources
Helixi498 – 51417Combined sources
Helixi526 – 54015Combined sources
Beta strandi543 – 5453Combined sources
Beta strandi560 – 5623Combined sources
Turni565 – 5684Combined sources
Beta strandi569 – 5713Combined sources
Turni574 – 5763Combined sources
Turni586 – 5883Combined sources
Helixi589 – 60012Combined sources
Helixi609 – 61810Combined sources
Helixi629 – 64012Combined sources
Helixi643 – 6453Combined sources
Helixi646 – 66722Combined sources
Helixi673 – 68210Combined sources
Helixi686 – 6949Combined sources
Helixi699 – 7079Combined sources
Beta strandi708 – 7103Combined sources
Helixi716 – 73318Combined sources
Helixi735 – 74511Combined sources
Helixi747 – 7493Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2FFLX-ray3.33A/B/C/D1-754[»]
2QVWX-ray3.00A/B/C/D1-754[»]
ProteinModelPortaliA8BQJ3.
SMRiA8BQJ3. Positions 1-754.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiA8BQJ3.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini123 – 251129PAZPROSITE-ProRule annotationAdd
BLAST
Domaini298 – 418121RNase III 1PROSITE-ProRule annotationAdd
BLAST
Domaini613 – 734122RNase III 2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 PAZ domain.PROSITE-ProRule annotation
Contains 2 RNase III domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

InParanoidiA8BQJ3.

Family and domain databases

CDDicd00593. RIBOc. 2 hits.
Gene3Di1.10.1520.10. 2 hits.
InterProiIPR003100. PAZ_dom.
IPR000999. RNase_III_dom.
[Graphical view]
PfamiPF02170. PAZ. 1 hit.
PF00636. Ribonuclease_3. 2 hits.
[Graphical view]
SMARTiSM00949. PAZ. 1 hit.
SM00535. RIBOc. 2 hits.
[Graphical view]
SUPFAMiSSF101690. SSF101690. 1 hit.
SSF69065. SSF69065. 2 hits.
PROSITEiPS50821. PAZ. 1 hit.
PS50142. RNASE_3_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A8BQJ3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHALGHCCTV VTTRGPSHWL LLLDTHLGTL PGFKVSAGRG LPAAEVYFEA
60 70 80 90 100
GPRVSLSRTD ATIVAVYQSI LFQLLGPTFP ASWTEIGATM PHNEYTFPRF
110 120 130 140 150
ISNPPQFATL AFLPLLSPTS PLDLRALMVT AQLMCDAKRL SDEYTDYSTL
160 170 180 190 200
SASLHGRMVA TPEISWSLYV VLGIDSTQTS LSYFTRANES ITYMRYYATA
210 220 230 240 250
HNIHLRAADL PLVAAVRLDD LKDHQIPAPG SWDDLAPKLR FLPPELCLLL
260 270 280 290 300
PDEFDLIRVQ ALQFLPEIAK HICDIQNTIC ALDKSFPDCG RIGGERYFAI
310 320 330 340 350
TAGLRLDQGR GRGLAGWRTP FGPFGVSHTD VFQRLELLGD AVLGFIVTAR
360 370 380 390 400
LLCLFPDASV GTLVELKMEL VRNEALNYLV QTLGLPQLAE FSNNLVAKSK
410 420 430 440 450
TWADMYEEIV GSIFTGPNGI YGCEEFLAKT LMSPEHSKTV GSACPDAVTK
460 470 480 490 500
ASKRVCMGEA GAHEFRSLVD YACEQGISVF CSSRVSTMFL ERLRDIPAED
510 520 530 540 550
MLDWYRLGIQ FSHRSGLSGP GGVVSVIDIM THLARGLWLG SPGFYVEQQT
560 570 580 590 600
DKNESACPPT IPVLYIYHRS VQCPVLYGSL TETPTGPVAS KVLALYEKIL
610 620 630 640 650
AYESSGGSKH IAAQTVSRSL AVPIPSGTIP FLIRLLQIAL TPHVYQKLEL
660 670 680 690 700
LGDAFLKCSL ALHLHALHPT LTEGALTRMR QSAETNSVLG RLTKRFPSVV
710 720 730 740 750
SEVIIESHPK IQPDSKVYGD TFEAILAAIL LACGEEAAGA FVREHVLPQV

VADA
Length:754
Mass (Da):82,478
Last modified:November 13, 2007 - v1
Checksum:i27FDC93E7C78BE0A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AACB02000033 Genomic DNA. Translation: EDO77862.1.
RefSeqiXP_001705536.1. XM_001705484.1.

Genome annotation databases

EnsemblProtistsiEDO77862; EDO77862; GL50803_103887.
GeneIDi5698409.
KEGGigla:GL50803_103887.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AACB02000033 Genomic DNA. Translation: EDO77862.1.
RefSeqiXP_001705536.1. XM_001705484.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2FFLX-ray3.33A/B/C/D1-754[»]
2QVWX-ray3.00A/B/C/D1-754[»]
ProteinModelPortaliA8BQJ3.
SMRiA8BQJ3. Positions 1-754.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi184922.XP_001705536.1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsiEDO77862; EDO77862; GL50803_103887.
GeneIDi5698409.
KEGGigla:GL50803_103887.

Organism-specific databases

EuPathDBiGiardiaDB:GL50803_103887.

Phylogenomic databases

InParanoidiA8BQJ3.

Miscellaneous databases

EvolutionaryTraceiA8BQJ3.

Family and domain databases

CDDicd00593. RIBOc. 2 hits.
Gene3Di1.10.1520.10. 2 hits.
InterProiIPR003100. PAZ_dom.
IPR000999. RNase_III_dom.
[Graphical view]
PfamiPF02170. PAZ. 1 hit.
PF00636. Ribonuclease_3. 2 hits.
[Graphical view]
SMARTiSM00949. PAZ. 1 hit.
SM00535. RIBOc. 2 hits.
[Graphical view]
SUPFAMiSSF101690. SSF101690. 1 hit.
SSF69065. SSF69065. 2 hits.
PROSITEiPS50821. PAZ. 1 hit.
PS50142. RNASE_3_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDCL_GIAIC
AccessioniPrimary (citable) accession number: A8BQJ3
Secondary accession number(s): Q86QW6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: November 13, 2007
Last modified: September 7, 2016
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.