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Protein

Endoribonuclease Dicer-like

Gene

GL50803_103887

Organism
Giardia intestinalis (strain ATCC 50803 / WB clone C6) (Giardia lamblia)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in cleaving double-stranded RNA in the RNA interference (RNAi) pathway. It produces 21 to 23 bp dsRNAs (siRNAs) which target the selective destruction of homologous RNAs.1 Publication

Cofactori

Mg2+1 Publication, Mn2+1 PublicationNote: Binds 2 magnesium or manganese ions per subunit.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi336Magnesium or manganese 1Combined sources2 Publications1
Metal bindingi404Magnesium or manganese 1Combined sources2 Publications1
Metal bindingi407Magnesium or manganese 1Combined sources2 Publications1
Metal bindingi649Magnesium or manganese 2Combined sources2 Publications1
Metal bindingi720Magnesium or manganese 2Combined sources2 Publications1
Metal bindingi723Magnesium or manganese 2Combined sources2 Publications1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

RNA-mediated gene silencing

Keywords - Ligandi

Magnesium, Manganese, Metal-binding, Nucleotide-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Endoribonuclease Dicer-like (EC:3.1.26.-1 Publication)
Gene namesi
ORF Names:GL50803_103887
OrganismiGiardia intestinalis (strain ATCC 50803 / WB clone C6) (Giardia lamblia)
Taxonomic identifieri184922 [NCBI]
Taxonomic lineageiEukaryotaDiplomonadidaHexamitidaeGiardiinaeGiardia
Proteomesi
  • UP000001548 Componenti: Unassembled WGS sequence

Organism-specific databases

EuPathDBiGiardiaDB:GL50803_103887.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003713031 – 754Endoribonuclease Dicer-likeAdd BLAST754

Interactioni

Subunit structurei

Homodimer.2 Publications

Protein-protein interaction databases

STRINGi184922.XP_001705536.1.

Structurei

Secondary structure

1754
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi4 – 11Combined sources8
Beta strandi16 – 25Combined sources10
Beta strandi33 – 35Combined sources3
Beta strandi39 – 41Combined sources3
Beta strandi44 – 47Combined sources4
Helixi58 – 75Combined sources18
Helixi83 – 88Combined sources6
Helixi92 – 94Combined sources3
Beta strandi95 – 97Combined sources3
Beta strandi110 – 117Combined sources8
Helixi125 – 135Combined sources11
Helixi140 – 142Combined sources3
Helixi144 – 146Combined sources3
Turni151 – 153Combined sources3
Beta strandi158 – 160Combined sources3
Turni162 – 166Combined sources5
Beta strandi168 – 179Combined sources12
Beta strandi182 – 186Combined sources5
Beta strandi189 – 192Combined sources4
Helixi193 – 199Combined sources7
Beta strandi210 – 217Combined sources8
Turni218 – 223Combined sources6
Beta strandi240 – 242Combined sources3
Turni244 – 246Combined sources3
Helixi258 – 262Combined sources5
Helixi265 – 282Combined sources18
Helixi283 – 285Combined sources3
Helixi293 – 304Combined sources12
Beta strandi313 – 315Combined sources3
Beta strandi318 – 320Combined sources3
Helixi323 – 325Combined sources3
Helixi328 – 354Combined sources27
Helixi360 – 371Combined sources12
Helixi373 – 382Combined sources10
Turni386 – 388Combined sources3
Helixi402 – 415Combined sources14
Helixi419 – 431Combined sources13
Helixi434 – 436Combined sources3
Helixi446 – 456Combined sources11
Helixi462 – 471Combined sources10
Turni472 – 476Combined sources5
Beta strandi480 – 483Combined sources4
Helixi484 – 495Combined sources12
Helixi498 – 514Combined sources17
Helixi526 – 540Combined sources15
Beta strandi543 – 545Combined sources3
Beta strandi560 – 562Combined sources3
Turni565 – 568Combined sources4
Beta strandi569 – 571Combined sources3
Turni574 – 576Combined sources3
Turni586 – 588Combined sources3
Helixi589 – 600Combined sources12
Helixi609 – 618Combined sources10
Helixi629 – 640Combined sources12
Helixi643 – 645Combined sources3
Helixi646 – 667Combined sources22
Helixi673 – 682Combined sources10
Helixi686 – 694Combined sources9
Helixi699 – 707Combined sources9
Beta strandi708 – 710Combined sources3
Helixi716 – 733Combined sources18
Helixi735 – 745Combined sources11
Helixi747 – 749Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2FFLX-ray3.33A/B/C/D1-754[»]
2QVWX-ray3.00A/B/C/D1-754[»]
ProteinModelPortaliA8BQJ3.
SMRiA8BQJ3.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiA8BQJ3.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini123 – 251PAZPROSITE-ProRule annotationAdd BLAST129
Domaini298 – 418RNase III 1PROSITE-ProRule annotationAdd BLAST121
Domaini613 – 734RNase III 2PROSITE-ProRule annotationAdd BLAST122

Sequence similaritiesi

Contains 1 PAZ domain.PROSITE-ProRule annotation
Contains 2 RNase III domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

InParanoidiA8BQJ3.

Family and domain databases

CDDicd00593. RIBOc. 2 hits.
Gene3Di1.10.1520.10. 2 hits.
InterProiIPR003100. PAZ_dom.
IPR000999. RNase_III_dom.
[Graphical view]
PfamiPF02170. PAZ. 1 hit.
PF00636. Ribonuclease_3. 2 hits.
[Graphical view]
SMARTiSM00949. PAZ. 1 hit.
SM00535. RIBOc. 2 hits.
[Graphical view]
SUPFAMiSSF101690. SSF101690. 1 hit.
SSF69065. SSF69065. 2 hits.
PROSITEiPS50821. PAZ. 1 hit.
PS50142. RNASE_3_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A8BQJ3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHALGHCCTV VTTRGPSHWL LLLDTHLGTL PGFKVSAGRG LPAAEVYFEA
60 70 80 90 100
GPRVSLSRTD ATIVAVYQSI LFQLLGPTFP ASWTEIGATM PHNEYTFPRF
110 120 130 140 150
ISNPPQFATL AFLPLLSPTS PLDLRALMVT AQLMCDAKRL SDEYTDYSTL
160 170 180 190 200
SASLHGRMVA TPEISWSLYV VLGIDSTQTS LSYFTRANES ITYMRYYATA
210 220 230 240 250
HNIHLRAADL PLVAAVRLDD LKDHQIPAPG SWDDLAPKLR FLPPELCLLL
260 270 280 290 300
PDEFDLIRVQ ALQFLPEIAK HICDIQNTIC ALDKSFPDCG RIGGERYFAI
310 320 330 340 350
TAGLRLDQGR GRGLAGWRTP FGPFGVSHTD VFQRLELLGD AVLGFIVTAR
360 370 380 390 400
LLCLFPDASV GTLVELKMEL VRNEALNYLV QTLGLPQLAE FSNNLVAKSK
410 420 430 440 450
TWADMYEEIV GSIFTGPNGI YGCEEFLAKT LMSPEHSKTV GSACPDAVTK
460 470 480 490 500
ASKRVCMGEA GAHEFRSLVD YACEQGISVF CSSRVSTMFL ERLRDIPAED
510 520 530 540 550
MLDWYRLGIQ FSHRSGLSGP GGVVSVIDIM THLARGLWLG SPGFYVEQQT
560 570 580 590 600
DKNESACPPT IPVLYIYHRS VQCPVLYGSL TETPTGPVAS KVLALYEKIL
610 620 630 640 650
AYESSGGSKH IAAQTVSRSL AVPIPSGTIP FLIRLLQIAL TPHVYQKLEL
660 670 680 690 700
LGDAFLKCSL ALHLHALHPT LTEGALTRMR QSAETNSVLG RLTKRFPSVV
710 720 730 740 750
SEVIIESHPK IQPDSKVYGD TFEAILAAIL LACGEEAAGA FVREHVLPQV

VADA
Length:754
Mass (Da):82,478
Last modified:November 13, 2007 - v1
Checksum:i27FDC93E7C78BE0A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AACB02000033 Genomic DNA. Translation: EDO77862.1.
RefSeqiXP_001705536.1. XM_001705484.1.

Genome annotation databases

EnsemblProtistsiEDO77862; EDO77862; GL50803_103887.
GeneIDi5698409.
KEGGigla:GL50803_103887.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AACB02000033 Genomic DNA. Translation: EDO77862.1.
RefSeqiXP_001705536.1. XM_001705484.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2FFLX-ray3.33A/B/C/D1-754[»]
2QVWX-ray3.00A/B/C/D1-754[»]
ProteinModelPortaliA8BQJ3.
SMRiA8BQJ3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi184922.XP_001705536.1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsiEDO77862; EDO77862; GL50803_103887.
GeneIDi5698409.
KEGGigla:GL50803_103887.

Organism-specific databases

EuPathDBiGiardiaDB:GL50803_103887.

Phylogenomic databases

InParanoidiA8BQJ3.

Miscellaneous databases

EvolutionaryTraceiA8BQJ3.

Family and domain databases

CDDicd00593. RIBOc. 2 hits.
Gene3Di1.10.1520.10. 2 hits.
InterProiIPR003100. PAZ_dom.
IPR000999. RNase_III_dom.
[Graphical view]
PfamiPF02170. PAZ. 1 hit.
PF00636. Ribonuclease_3. 2 hits.
[Graphical view]
SMARTiSM00949. PAZ. 1 hit.
SM00535. RIBOc. 2 hits.
[Graphical view]
SUPFAMiSSF101690. SSF101690. 1 hit.
SSF69065. SSF69065. 2 hits.
PROSITEiPS50821. PAZ. 1 hit.
PS50142. RNASE_3_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDCL_GIAIC
AccessioniPrimary (citable) accession number: A8BQJ3
Secondary accession number(s): Q86QW6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: November 13, 2007
Last modified: November 2, 2016
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.