A8BQB4 (GDE_HORSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycogen debranching enzyme

Alternative name(s):
Glycogen debrancher

Including the following 2 domains:

1. 4-alpha-glucanotransferase
   EC=2.4.1.25
   Alternative name(s):
   Oligo-1,4-1,4-glucantransferase
2. Amylo-alpha-1,6-glucosidase
   Short name=Amylo-1,6-glucosidase
   EC=3.2.1.33
   Alternative name(s):
   Dextrin 6-alpha-D-glucosidase

Gene names

Name:

AGL

Organism

Equus caballus (Horse) [Reference proteome]

Taxonomic identifier

9796 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Perissodactyla › Equidae › Equus › Equus

Protein attributes

Sequence length	1533 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Multifunctional enzyme acting as 1,4-alpha-D-glucan:1,4-alpha-D-glucan 4-alpha-D-glycosyltransferase and amylo-1,6-glucosidase in glycogen degradation By similarity. UniProtKB P35573
Catalytic activity	Transfers a segment of a (1->4)-alpha-D-glucan to a new position in an acceptor, which may be glucose or a (1->4)-alpha-D-glucan. UniProtKB P35573 Hydrolysis of (1->6)-alpha-D-glucosidic branch linkages in glycogen phosphorylase limit dextrin. UniProtKB P35573
Subunit structure	Monomer. Interacts with NHLRC1/malin By similarity. UniProtKB P35573
Subcellular location	Cytoplasm By similarity. Note: Under glycogenolytic conditions localizes to the nucleus By similarity. UniProtKB P35573
Tissue specificity	Ubiquitous. Expressed in striated skeletal muscle, heart, liver, spleen, skin, spinal cord, lung, kidney and testicle. Ref.1
Post-translational modification	Ubiquitinated By similarity. UniProtKB P35573
Sequence similarities	Belongs to the glycogen debranching enzyme family.

Ontologies

Keywords
Biological process	Glycogen biosynthesis
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing Polymorphism
Molecular function	Glycosidase Glycosyltransferase Hydrolase Transferase
PTM	Ubl conjugation
Technical term	Complete proteome Multifunctional enzyme Reference proteome
Gene Ontology (GO)
Biological_process	glycogen biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	4-alpha-glucanotransferase activity Inferred from electronic annotation. Source: EC amylo-alpha-1,6-glucosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 Ref.1 (identifier: A8BQB4-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 Ref.1 (identifier: A8BQB4-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-156: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1533	1533	Glycogen debranching enzyme		PRO_0000401163
Regions
	Region	1 – ?		4-alpha-glucanotransferase UniProtKB P35573
	Region	? – 1533		Amylo-1,6-glucosidase UniProtKB P35573
Sites
	Active site	527	1	By similarity UniProtKB P35573
	Active site	530	1	By similarity UniProtKB P35573
	Active site	628	1	By similarity UniProtKB P35573
Natural variations
	Alternative sequence	1 – 156	156	Missing in isoform 2. Ref.1		VSP_040070
	Natural variant	326	1	S → T. Ref.1
	Natural variant	368	1	I → L. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 13, 2007. Version 1. Checksum: C19B5C7C192302F3	FASTA	1,533	174,666
	10 20 30 40 50 60 MGHSKQIRTL LLNEMEKLEK TLFRLEQGFE LQFRLGPTLQ GKAVTVYTNY PFPGETFNRE 70 80 90 100 110 120 KFHSLQWENP TEREDDSDKY CKLNLQQAGS FQYYFLQGNE KSGGGYIVVD PILRVGADNH 130 140 150 160 170 180 VLPLDCVTLQ TFLTKCLGPF DEWESRLRVA KESGYNMIHF TPLQTLGLSR SSYSLADQLE 190 200 210 220 230 240 LNPDFSRPNK KYTWHDVGQL VEKLKKEWDI LCITDVVYNH TAANSKWIHE HPESAYNLVN 250 260 270 280 290 300 SPHLKPAWVL DRALWHLSCD VAEGKYREKG VPALIENDHQ MNCIRKIIWE DIYPKIHLWE 310 320 330 340 350 360 FFQVDVHKAV EQFRGLLTQE NRKIISQPDP KQHLKIIQDP EYRRLGCTVD MNIALATFIP 370 380 390 400 410 420 HDNGPAAIDE CCNWFRKRIE ELNAEKHQLV NYHQEQAVNC LLGNVFYERL AGHGPKLGPV 430 440 450 460 470 480 TRKHPLVTRY FTFPFEEMTP STEESMIHLP NKACFLMAHN GWVMGDDPLR NFAEPGSDVY 490 500 510 520 530 540 LRRELICWGD SVKLRYGNKP EDCPYLWAHM KKYTEITATH FQGVRLDNCH STPIHVAEYM 550 560 570 580 590 600 LDAARKLQPN LYVVAELFTG SEDLDNIFVT RLGISSLIRE AMSAADSHEE GRLVYRYGGE 610 620 630 640 650 660 PVGSFVQPCL RPLMPAIAHA LFMDITHDNE CPIVHRSAYD ALPSSTIVSM ASCASGSTKG 670 680 690 700 710 720 YDELVPHQIS VVSEERFYTK WNPEALPSNT GEVNFQSGII AARRAINKLH QELGAKGFIQ 730 740 750 760 770 780 VYVDQVDQDI VAVTRHSPSI HQSVVSVSRT AFRNPKTSFY SKEVPHMYIP GKIEEVVLEA 790 800 810 820 830 840 RTIERHTIPY KKDENSINGM PDITVEIREH IQLNESKIVK HAGIVTKGPN EFVQEIEFEN 850 860 870 880 890 900 LTPGSVIIFR VSLDPHAQVA VGILRNHLTQ FSPHFKSGSL AVDNADPILK IPFASIASKL 910 920 930 940 950 960 TLAELNQVLY RCESEEQEDG GGCYNIPNWS SLKYAGLQGL MSILAEIRPR NDLGHPFCDN 970 980 990 1000 1010 1020 LRSGDWMIDY VSSRLISRSG TIAEVGKWLQ AMFLYLKQIP RYLIPCYFDA ILIGAYTTLL 1030 1040 1050 1060 1070 1080 DIAWKQMSSF VQNGSTFVKH LSLGSVQMCG VGKFPSLPLL SPSLTDLPYR VNEITKEKEQ 1090 1100 1110 1120 1130 1140 CCGSLAAGLP HFSAGIFRCW GRDTFIALRG LLLVTGRYLE ARNIILAFAG TLRHGLIPNL 1150 1160 1170 1180 1190 1200 LGEGTHARYN CRDAVWWWLQ CIQDYCKIVP NGLDILRCPV SRMYPTDDSV PLSAGTVDQP 1210 1220 1230 1240 1250 1260 LFEVIQEAMQ RHVQGIQFRE RNAGPQIDRN MKDEGFNITA GVDEETGFVY GGNRFNCGTW 1270 1280 1290 1300 1310 1320 MDKMGESDRA RNRGIPATPR DGSAVEIVGL SKSAVRWLLE LSRKNIFPYH EVRVKRHGKF 1330 1340 1350 1360 1370 1380 VTVSYDEWNR KIQDNFEKLF HVSEDPSDFN EKHPELVHKR GIYKDSYGAS SPWCDYQLRP 1390 1400 1410 1420 1430 1440 NFTIAMVVAP ELFTPEKAWK ALEIAEKKLL GPLGMKTLDP DDMVYCGIYD NALDNDNYNL 1450 1460 1470 1480 1490 1500 AKGFNYHQGP EWLWPTGYFL RAKLYFSKLM GPETNAKTMF LVKNVLSRHY VHLERSPWKG 1510 1520 1530 LPELTNENGQ YCPFSCETQA WSIATVLETL YDL
	Isoform 2 [UniParc]. Checksum: B2E718893457946E	FASTA	1,377	156,636
	10 20 30 40 50 60 MIHFTPLQTL GLSRSSYSLA DQLELNPDFS RPNKKYTWHD VGQLVEKLKK EWDILCITDV 70 80 90 100 110 120 VYNHTAANSK WIHEHPESAY NLVNSPHLKP AWVLDRALWH LSCDVAEGKY REKGVPALIE 130 140 150 160 170 180 NDHQMNCIRK IIWEDIYPKI HLWEFFQVDV HKAVEQFRGL LTQENRKIIS QPDPKQHLKI 190 200 210 220 230 240 IQDPEYRRLG CTVDMNIALA TFIPHDNGPA AIDECCNWFR KRIEELNAEK HQLVNYHQEQ 250 260 270 280 290 300 AVNCLLGNVF YERLAGHGPK LGPVTRKHPL VTRYFTFPFE EMTPSTEESM IHLPNKACFL 310 320 330 340 350 360 MAHNGWVMGD DPLRNFAEPG SDVYLRRELI CWGDSVKLRY GNKPEDCPYL WAHMKKYTEI 370 380 390 400 410 420 TATHFQGVRL DNCHSTPIHV AEYMLDAARK LQPNLYVVAE LFTGSEDLDN IFVTRLGISS 430 440 450 460 470 480 LIREAMSAAD SHEEGRLVYR YGGEPVGSFV QPCLRPLMPA IAHALFMDIT HDNECPIVHR 490 500 510 520 530 540 SAYDALPSST IVSMASCASG STKGYDELVP HQISVVSEER FYTKWNPEAL PSNTGEVNFQ 550 560 570 580 590 600 SGIIAARRAI NKLHQELGAK GFIQVYVDQV DQDIVAVTRH SPSIHQSVVS VSRTAFRNPK 610 620 630 640 650 660 TSFYSKEVPH MYIPGKIEEV VLEARTIERH TIPYKKDENS INGMPDITVE IREHIQLNES 670 680 690 700 710 720 KIVKHAGIVT KGPNEFVQEI EFENLTPGSV IIFRVSLDPH AQVAVGILRN HLTQFSPHFK 730 740 750 760 770 780 SGSLAVDNAD PILKIPFASI ASKLTLAELN QVLYRCESEE QEDGGGCYNI PNWSSLKYAG 790 800 810 820 830 840 LQGLMSILAE IRPRNDLGHP FCDNLRSGDW MIDYVSSRLI SRSGTIAEVG KWLQAMFLYL 850 860 870 880 890 900 KQIPRYLIPC YFDAILIGAY TTLLDIAWKQ MSSFVQNGST FVKHLSLGSV QMCGVGKFPS 910 920 930 940 950 960 LPLLSPSLTD LPYRVNEITK EKEQCCGSLA AGLPHFSAGI FRCWGRDTFI ALRGLLLVTG 970 980 990 1000 1010 1020 RYLEARNIIL AFAGTLRHGL IPNLLGEGTH ARYNCRDAVW WWLQCIQDYC KIVPNGLDIL 1030 1040 1050 1060 1070 1080 RCPVSRMYPT DDSVPLSAGT VDQPLFEVIQ EAMQRHVQGI QFRERNAGPQ IDRNMKDEGF 1090 1100 1110 1120 1130 1140 NITAGVDEET GFVYGGNRFN CGTWMDKMGE SDRARNRGIP ATPRDGSAVE IVGLSKSAVR 1150 1160 1170 1180 1190 1200 WLLELSRKNI FPYHEVRVKR HGKFVTVSYD EWNRKIQDNF EKLFHVSEDP SDFNEKHPEL 1210 1220 1230 1240 1250 1260 VHKRGIYKDS YGASSPWCDY QLRPNFTIAM VVAPELFTPE KAWKALEIAE KKLLGPLGMK 1270 1280 1290 1300 1310 1320 TLDPDDMVYC GIYDNALDND NYNLAKGFNY HQGPEWLWPT GYFLRAKLYF SKLMGPETNA 1330 1340 1350 1360 1370 KTMFLVKNVL SRHYVHLERS PWKGLPELTN ENGQYCPFSC ETQAWSIATV LETLYDL

References

[1]

"Characterization of the equine glycogen debranching enzyme gene (AGL): Genomic and cDNA structure, localization, polymorphism and expression." Herszberg B., Mata X., Giulotto E., Decaunes P., Piras F.M., Chowdhary B.P., Chaffaux S., Guerin G. Gene 404:1-9(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS 1 AND 2), TISSUE SPECIFICITY, VARIANTS THR-326 AND LEU-368.

Cross-references

Sequence databases
EMBL GenBank DDBJ	EF660736 Genomic DNA. Translation: ABV45394.1. EF660736 Genomic DNA. Translation: ABV45395.1. EF660736 Genomic DNA. Translation: ABV74211.1. EF660736 Genomic DNA. Translation: ABV74212.1. EF660736 Genomic DNA. Translation: ABV74213.1. EF660737 mRNA. Translation: ABV45396.1. EF660738 mRNA. Translation: ABV45397.1. EF660739 mRNA. Translation: ABV45398.1. EF660740 mRNA. Translation: ABV45399.1. EF660741 mRNA. Translation: ABV45400.1.
RefSeq	NP_001103778.1. NM_001110308.1.
UniGene	Eca.16037.
3D structure databases
ProteinModelPortal	A8BQB4.
ModBase	Search...
Protein-protein interaction databases
STRING	9796.ENSECAP00000009911.
Protein family/group databases
CAZy	GH13. Glycoside Hydrolase Family 13.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	100050695.
KEGG	ecb:100050695.
Organism-specific databases
CTD	178.
Phylogenomic databases
eggNOG	COG3408.
HOGENOM	HOG000212981.
HOVERGEN	HBG005824.
InParanoid	A8BQB4.
KO	K01196.
OrthoDB	EOG4HT8RB.
Family and domain databases
Gene3D	3.20.20.80. 5 hits.
InterPro	IPR008928. 6-hairpin_glycosidase-like. IPR010401. GDE_C. IPR013781. Glyco_hydro_catalytic_dom. IPR006421. Glycogen_debranch_met. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
PANTHER	PTHR10569. PTHR10569. 1 hit.
Pfam	PF06202. GDE_C. 1 hit. [Graphical view]
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit. SSF48208. Glyco_trans_6hp. 1 hit.
TIGRFAMs	TIGR01531. glyc_debranch. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GDE_HORSE

Accession

Primary (citable) accession number: A8BQB4
Secondary accession number(s): A8BQB7

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 30, 2010
Last sequence update:	November 13, 2007
Last modified:	May 1, 2013
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program