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A8BQB4

- GDE_HORSE

UniProt

A8BQB4 - GDE_HORSE

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Protein

Glycogen debranching enzyme

Gene

AGL

Organism
Equus caballus (Horse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Multifunctional enzyme acting as 1,4-alpha-D-glucan:1,4-alpha-D-glucan 4-alpha-D-glycosyltransferase and amylo-1,6-glucosidase in glycogen degradation.By similarity

Catalytic activityi

Transfers a segment of a (1->4)-alpha-D-glucan to a new position in an acceptor, which may be glucose or a (1->4)-alpha-D-glucan.By similarity
Hydrolysis of (1->6)-alpha-D-glucosidic branch linkages in glycogen phosphorylase limit dextrin.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei527 – 5271By similarity
Active sitei530 – 5301By similarity
Active sitei628 – 6281By similarity

GO - Molecular functioni

  1. 4-alpha-glucanotransferase activity Source: UniProtKB-EC
  2. amylo-alpha-1,6-glucosidase activity Source: UniProtKB-EC

GO - Biological processi

  1. glycogen biosynthetic process Source: UniProtKB-KW
  2. glycogen catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Glycosyltransferase, Hydrolase, Transferase

Keywords - Biological processi

Glycogen biosynthesis

Protein family/group databases

CAZyiGH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycogen debranching enzymeBy similarity
Alternative name(s):
Glycogen debrancherBy similarity
Including the following 2 domains:
4-alpha-glucanotransferaseBy similarity (EC:2.4.1.25)
Alternative name(s):
Oligo-1,4-1,4-glucantransferaseBy similarity
Amylo-alpha-1,6-glucosidaseBy similarity (EC:3.2.1.33)
Short name:
Amylo-1,6-glucosidaseBy similarity
Alternative name(s):
Dextrin 6-alpha-D-glucosidaseBy similarity
Gene namesi
Name:AGLImported
OrganismiEquus caballus (Horse)
Taxonomic identifieri9796 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaPerissodactylaEquidaeEquus
ProteomesiUP000002281: Unplaced

Subcellular locationi

Cytoplasm By similarity
Note: Under glycogenolytic conditions localizes to the nucleus.By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 15331533Glycogen debranching enzymePRO_0000401163Add
BLAST

Post-translational modificationi

Ubiquitinated.By similarity

Keywords - PTMi

Ubl conjugation

Expressioni

Tissue specificityi

Ubiquitous. Expressed in striated skeletal muscle, heart, liver, spleen, skin, spinal cord, lung, kidney and testicle.1 Publication

Interactioni

Subunit structurei

Monomer. Interacts with NHLRC1/malin (By similarity).By similarity

Protein-protein interaction databases

STRINGi9796.ENSECAP00000009911.

Structurei

3D structure databases

ProteinModelPortaliA8BQB4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni? – 1533Amylo-1,6-glucosidaseBy similarity
Regioni1 – ?4-alpha-glucanotransferaseBy similarity

Sequence similaritiesi

Belongs to the glycogen debranching enzyme family.Sequence Analysis

Phylogenomic databases

eggNOGiCOG3408.
HOGENOMiHOG000212981.
HOVERGENiHBG005824.
InParanoidiA8BQB4.
KOiK01196.

Family and domain databases

Gene3Di3.20.20.80. 5 hits.
InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR010401. AGL/Gdb1.
IPR029436. AGL_euk_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR006421. Glycogen_debranch_met.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10569. PTHR10569. 1 hit.
PfamiPF06202. GDE_C. 1 hit.
PF14699. hGDE_N. 1 hit.
[Graphical view]
SUPFAMiSSF48208. SSF48208. 2 hits.
SSF51445. SSF51445. 2 hits.
TIGRFAMsiTIGR01531. glyc_debranch. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 11 Publication (identifier: A8BQB4-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGHSKQIRTL LLNEMEKLEK TLFRLEQGFE LQFRLGPTLQ GKAVTVYTNY
60 70 80 90 100
PFPGETFNRE KFHSLQWENP TEREDDSDKY CKLNLQQAGS FQYYFLQGNE
110 120 130 140 150
KSGGGYIVVD PILRVGADNH VLPLDCVTLQ TFLTKCLGPF DEWESRLRVA
160 170 180 190 200
KESGYNMIHF TPLQTLGLSR SSYSLADQLE LNPDFSRPNK KYTWHDVGQL
210 220 230 240 250
VEKLKKEWDI LCITDVVYNH TAANSKWIHE HPESAYNLVN SPHLKPAWVL
260 270 280 290 300
DRALWHLSCD VAEGKYREKG VPALIENDHQ MNCIRKIIWE DIYPKIHLWE
310 320 330 340 350
FFQVDVHKAV EQFRGLLTQE NRKIISQPDP KQHLKIIQDP EYRRLGCTVD
360 370 380 390 400
MNIALATFIP HDNGPAAIDE CCNWFRKRIE ELNAEKHQLV NYHQEQAVNC
410 420 430 440 450
LLGNVFYERL AGHGPKLGPV TRKHPLVTRY FTFPFEEMTP STEESMIHLP
460 470 480 490 500
NKACFLMAHN GWVMGDDPLR NFAEPGSDVY LRRELICWGD SVKLRYGNKP
510 520 530 540 550
EDCPYLWAHM KKYTEITATH FQGVRLDNCH STPIHVAEYM LDAARKLQPN
560 570 580 590 600
LYVVAELFTG SEDLDNIFVT RLGISSLIRE AMSAADSHEE GRLVYRYGGE
610 620 630 640 650
PVGSFVQPCL RPLMPAIAHA LFMDITHDNE CPIVHRSAYD ALPSSTIVSM
660 670 680 690 700
ASCASGSTKG YDELVPHQIS VVSEERFYTK WNPEALPSNT GEVNFQSGII
710 720 730 740 750
AARRAINKLH QELGAKGFIQ VYVDQVDQDI VAVTRHSPSI HQSVVSVSRT
760 770 780 790 800
AFRNPKTSFY SKEVPHMYIP GKIEEVVLEA RTIERHTIPY KKDENSINGM
810 820 830 840 850
PDITVEIREH IQLNESKIVK HAGIVTKGPN EFVQEIEFEN LTPGSVIIFR
860 870 880 890 900
VSLDPHAQVA VGILRNHLTQ FSPHFKSGSL AVDNADPILK IPFASIASKL
910 920 930 940 950
TLAELNQVLY RCESEEQEDG GGCYNIPNWS SLKYAGLQGL MSILAEIRPR
960 970 980 990 1000
NDLGHPFCDN LRSGDWMIDY VSSRLISRSG TIAEVGKWLQ AMFLYLKQIP
1010 1020 1030 1040 1050
RYLIPCYFDA ILIGAYTTLL DIAWKQMSSF VQNGSTFVKH LSLGSVQMCG
1060 1070 1080 1090 1100
VGKFPSLPLL SPSLTDLPYR VNEITKEKEQ CCGSLAAGLP HFSAGIFRCW
1110 1120 1130 1140 1150
GRDTFIALRG LLLVTGRYLE ARNIILAFAG TLRHGLIPNL LGEGTHARYN
1160 1170 1180 1190 1200
CRDAVWWWLQ CIQDYCKIVP NGLDILRCPV SRMYPTDDSV PLSAGTVDQP
1210 1220 1230 1240 1250
LFEVIQEAMQ RHVQGIQFRE RNAGPQIDRN MKDEGFNITA GVDEETGFVY
1260 1270 1280 1290 1300
GGNRFNCGTW MDKMGESDRA RNRGIPATPR DGSAVEIVGL SKSAVRWLLE
1310 1320 1330 1340 1350
LSRKNIFPYH EVRVKRHGKF VTVSYDEWNR KIQDNFEKLF HVSEDPSDFN
1360 1370 1380 1390 1400
EKHPELVHKR GIYKDSYGAS SPWCDYQLRP NFTIAMVVAP ELFTPEKAWK
1410 1420 1430 1440 1450
ALEIAEKKLL GPLGMKTLDP DDMVYCGIYD NALDNDNYNL AKGFNYHQGP
1460 1470 1480 1490 1500
EWLWPTGYFL RAKLYFSKLM GPETNAKTMF LVKNVLSRHY VHLERSPWKG
1510 1520 1530
LPELTNENGQ YCPFSCETQA WSIATVLETL YDL
Length:1,533
Mass (Da):174,666
Last modified:November 13, 2007 - v1
Checksum:iC19B5C7C192302F3
GO
Isoform 21 Publication (identifier: A8BQB4-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-156: Missing.

Note: No experimental confirmation available.Curated

Show »
Length:1,377
Mass (Da):156,636
Checksum:iB2E718893457946E
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti326 – 3261S → T.1 Publication
Natural varianti368 – 3681I → L.1 Publication

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 156156Missing in isoform 2. 1 PublicationVSP_040070Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EF660736 Genomic DNA. Translation: ABV45394.1.
EF660736 Genomic DNA. Translation: ABV45395.1.
EF660736 Genomic DNA. Translation: ABV74211.1.
EF660736 Genomic DNA. Translation: ABV74212.1.
EF660736 Genomic DNA. Translation: ABV74213.1.
EF660737 mRNA. Translation: ABV45396.1.
EF660738 mRNA. Translation: ABV45397.1.
EF660739 mRNA. Translation: ABV45398.1.
EF660740 mRNA. Translation: ABV45399.1.
EF660741 mRNA. Translation: ABV45400.1.
RefSeqiNP_001103778.1. NM_001110308.1. [A8BQB4-1]
UniGeneiEca.16037.

Genome annotation databases

GeneIDi100050695.
KEGGiecb:100050695.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EF660736 Genomic DNA. Translation: ABV45394.1 .
EF660736 Genomic DNA. Translation: ABV45395.1 .
EF660736 Genomic DNA. Translation: ABV74211.1 .
EF660736 Genomic DNA. Translation: ABV74212.1 .
EF660736 Genomic DNA. Translation: ABV74213.1 .
EF660737 mRNA. Translation: ABV45396.1 .
EF660738 mRNA. Translation: ABV45397.1 .
EF660739 mRNA. Translation: ABV45398.1 .
EF660740 mRNA. Translation: ABV45399.1 .
EF660741 mRNA. Translation: ABV45400.1 .
RefSeqi NP_001103778.1. NM_001110308.1. [A8BQB4-1 ]
UniGenei Eca.16037.

3D structure databases

ProteinModelPortali A8BQB4.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9796.ENSECAP00000009911.

Protein family/group databases

CAZyi GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 100050695.
KEGGi ecb:100050695.

Organism-specific databases

CTDi 178.

Phylogenomic databases

eggNOGi COG3408.
HOGENOMi HOG000212981.
HOVERGENi HBG005824.
InParanoidi A8BQB4.
KOi K01196.

Family and domain databases

Gene3Di 3.20.20.80. 5 hits.
InterProi IPR008928. 6-hairpin_glycosidase-like.
IPR010401. AGL/Gdb1.
IPR029436. AGL_euk_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR006421. Glycogen_debranch_met.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
PANTHERi PTHR10569. PTHR10569. 1 hit.
Pfami PF06202. GDE_C. 1 hit.
PF14699. hGDE_N. 1 hit.
[Graphical view ]
SUPFAMi SSF48208. SSF48208. 2 hits.
SSF51445. SSF51445. 2 hits.
TIGRFAMsi TIGR01531. glyc_debranch. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Characterization of the equine glycogen debranching enzyme gene (AGL): Genomic and cDNA structure, localization, polymorphism and expression."
    Herszberg B., Mata X., Giulotto E., Decaunes P., Piras F.M., Chowdhary B.P., Chaffaux S., Guerin G.
    Gene 404:1-9(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS 1 AND 2), TISSUE SPECIFICITY, VARIANTS THR-326 AND LEU-368.

Entry informationi

Entry nameiGDE_HORSE
AccessioniPrimary (citable) accession number: A8BQB4
Secondary accession number(s): A8BQB7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 30, 2010
Last sequence update: November 13, 2007
Last modified: October 29, 2014
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3