Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

A8BQB4

- GDE_HORSE

UniProt

A8BQB4 - GDE_HORSE

Protein

Glycogen debranching enzyme

Gene

AGL

Organism
Equus caballus (Horse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 43 (01 Oct 2014)
      Sequence version 1 (13 Nov 2007)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Multifunctional enzyme acting as 1,4-alpha-D-glucan:1,4-alpha-D-glucan 4-alpha-D-glycosyltransferase and amylo-1,6-glucosidase in glycogen degradation.By similarity

    Catalytic activityi

    Transfers a segment of a (1->4)-alpha-D-glucan to a new position in an acceptor, which may be glucose or a (1->4)-alpha-D-glucan.By similarity
    Hydrolysis of (1->6)-alpha-D-glucosidic branch linkages in glycogen phosphorylase limit dextrin.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei527 – 5271By similarity
    Active sitei530 – 5301By similarity
    Active sitei628 – 6281By similarity

    GO - Molecular functioni

    1. 4-alpha-glucanotransferase activity Source: UniProtKB-EC
    2. amylo-alpha-1,6-glucosidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. glycogen biosynthetic process Source: UniProtKB-KW
    2. glycogen catabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Glycosyltransferase, Hydrolase, Transferase

    Keywords - Biological processi

    Glycogen biosynthesis

    Protein family/group databases

    CAZyiGH13. Glycoside Hydrolase Family 13.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glycogen debranching enzymeBy similarity
    Alternative name(s):
    Glycogen debrancherBy similarity
    Including the following 2 domains:
    4-alpha-glucanotransferaseBy similarity (EC:2.4.1.25)
    Alternative name(s):
    Oligo-1,4-1,4-glucantransferaseBy similarity
    Amylo-alpha-1,6-glucosidaseBy similarity (EC:3.2.1.33)
    Short name:
    Amylo-1,6-glucosidaseBy similarity
    Alternative name(s):
    Dextrin 6-alpha-D-glucosidaseBy similarity
    Gene namesi
    Name:AGLImported
    OrganismiEquus caballus (Horse)
    Taxonomic identifieri9796 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaPerissodactylaEquidaeEquus
    ProteomesiUP000002281: Unplaced

    Subcellular locationi

    Cytoplasm By similarity
    Note: Under glycogenolytic conditions localizes to the nucleus.By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 15331533Glycogen debranching enzymePRO_0000401163Add
    BLAST

    Post-translational modificationi

    Ubiquitinated.By similarity

    Keywords - PTMi

    Ubl conjugation

    Expressioni

    Tissue specificityi

    Ubiquitous. Expressed in striated skeletal muscle, heart, liver, spleen, skin, spinal cord, lung, kidney and testicle.1 Publication

    Interactioni

    Subunit structurei

    Monomer. Interacts with NHLRC1/malin By similarity.By similarity

    Protein-protein interaction databases

    STRINGi9796.ENSECAP00000009911.

    Structurei

    3D structure databases

    ProteinModelPortaliA8BQB4.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni? – 1533Amylo-1,6-glucosidaseBy similarity
    Regioni1 – ?4-alpha-glucanotransferaseBy similarity

    Sequence similaritiesi

    Belongs to the glycogen debranching enzyme family.Sequence Analysis

    Phylogenomic databases

    eggNOGiCOG3408.
    HOGENOMiHOG000212981.
    HOVERGENiHBG005824.
    InParanoidiA8BQB4.
    KOiK01196.

    Family and domain databases

    Gene3Di3.20.20.80. 5 hits.
    InterProiIPR008928. 6-hairpin_glycosidase-like.
    IPR010401. AGL/Gdb1.
    IPR029436. AGL_euk_N.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR006421. Glycogen_debranch_met.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PANTHERiPTHR10569. PTHR10569. 1 hit.
    PfamiPF06202. GDE_C. 1 hit.
    PF14699. hGDE_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF48208. SSF48208. 2 hits.
    SSF51445. SSF51445. 2 hits.
    TIGRFAMsiTIGR01531. glyc_debranch. 1 hit.

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 11 Publication (identifier: A8BQB4-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MGHSKQIRTL LLNEMEKLEK TLFRLEQGFE LQFRLGPTLQ GKAVTVYTNY     50
    PFPGETFNRE KFHSLQWENP TEREDDSDKY CKLNLQQAGS FQYYFLQGNE 100
    KSGGGYIVVD PILRVGADNH VLPLDCVTLQ TFLTKCLGPF DEWESRLRVA 150
    KESGYNMIHF TPLQTLGLSR SSYSLADQLE LNPDFSRPNK KYTWHDVGQL 200
    VEKLKKEWDI LCITDVVYNH TAANSKWIHE HPESAYNLVN SPHLKPAWVL 250
    DRALWHLSCD VAEGKYREKG VPALIENDHQ MNCIRKIIWE DIYPKIHLWE 300
    FFQVDVHKAV EQFRGLLTQE NRKIISQPDP KQHLKIIQDP EYRRLGCTVD 350
    MNIALATFIP HDNGPAAIDE CCNWFRKRIE ELNAEKHQLV NYHQEQAVNC 400
    LLGNVFYERL AGHGPKLGPV TRKHPLVTRY FTFPFEEMTP STEESMIHLP 450
    NKACFLMAHN GWVMGDDPLR NFAEPGSDVY LRRELICWGD SVKLRYGNKP 500
    EDCPYLWAHM KKYTEITATH FQGVRLDNCH STPIHVAEYM LDAARKLQPN 550
    LYVVAELFTG SEDLDNIFVT RLGISSLIRE AMSAADSHEE GRLVYRYGGE 600
    PVGSFVQPCL RPLMPAIAHA LFMDITHDNE CPIVHRSAYD ALPSSTIVSM 650
    ASCASGSTKG YDELVPHQIS VVSEERFYTK WNPEALPSNT GEVNFQSGII 700
    AARRAINKLH QELGAKGFIQ VYVDQVDQDI VAVTRHSPSI HQSVVSVSRT 750
    AFRNPKTSFY SKEVPHMYIP GKIEEVVLEA RTIERHTIPY KKDENSINGM 800
    PDITVEIREH IQLNESKIVK HAGIVTKGPN EFVQEIEFEN LTPGSVIIFR 850
    VSLDPHAQVA VGILRNHLTQ FSPHFKSGSL AVDNADPILK IPFASIASKL 900
    TLAELNQVLY RCESEEQEDG GGCYNIPNWS SLKYAGLQGL MSILAEIRPR 950
    NDLGHPFCDN LRSGDWMIDY VSSRLISRSG TIAEVGKWLQ AMFLYLKQIP 1000
    RYLIPCYFDA ILIGAYTTLL DIAWKQMSSF VQNGSTFVKH LSLGSVQMCG 1050
    VGKFPSLPLL SPSLTDLPYR VNEITKEKEQ CCGSLAAGLP HFSAGIFRCW 1100
    GRDTFIALRG LLLVTGRYLE ARNIILAFAG TLRHGLIPNL LGEGTHARYN 1150
    CRDAVWWWLQ CIQDYCKIVP NGLDILRCPV SRMYPTDDSV PLSAGTVDQP 1200
    LFEVIQEAMQ RHVQGIQFRE RNAGPQIDRN MKDEGFNITA GVDEETGFVY 1250
    GGNRFNCGTW MDKMGESDRA RNRGIPATPR DGSAVEIVGL SKSAVRWLLE 1300
    LSRKNIFPYH EVRVKRHGKF VTVSYDEWNR KIQDNFEKLF HVSEDPSDFN 1350
    EKHPELVHKR GIYKDSYGAS SPWCDYQLRP NFTIAMVVAP ELFTPEKAWK 1400
    ALEIAEKKLL GPLGMKTLDP DDMVYCGIYD NALDNDNYNL AKGFNYHQGP 1450
    EWLWPTGYFL RAKLYFSKLM GPETNAKTMF LVKNVLSRHY VHLERSPWKG 1500
    LPELTNENGQ YCPFSCETQA WSIATVLETL YDL 1533
    Length:1,533
    Mass (Da):174,666
    Last modified:November 13, 2007 - v1
    Checksum:iC19B5C7C192302F3
    GO
    Isoform 21 Publication (identifier: A8BQB4-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-156: Missing.

    Note: No experimental confirmation available.Curated

    Show »
    Length:1,377
    Mass (Da):156,636
    Checksum:iB2E718893457946E
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti326 – 3261S → T.1 Publication
    Natural varianti368 – 3681I → L.1 Publication

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 156156Missing in isoform 2. 1 PublicationVSP_040070Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    EF660736 Genomic DNA. Translation: ABV45394.1.
    EF660736 Genomic DNA. Translation: ABV45395.1.
    EF660736 Genomic DNA. Translation: ABV74211.1.
    EF660736 Genomic DNA. Translation: ABV74212.1.
    EF660736 Genomic DNA. Translation: ABV74213.1.
    EF660737 mRNA. Translation: ABV45396.1.
    EF660738 mRNA. Translation: ABV45397.1.
    EF660739 mRNA. Translation: ABV45398.1.
    EF660740 mRNA. Translation: ABV45399.1.
    EF660741 mRNA. Translation: ABV45400.1.
    RefSeqiNP_001103778.1. NM_001110308.1. [A8BQB4-1]
    UniGeneiEca.16037.

    Genome annotation databases

    GeneIDi100050695.
    KEGGiecb:100050695.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    EF660736 Genomic DNA. Translation: ABV45394.1 .
    EF660736 Genomic DNA. Translation: ABV45395.1 .
    EF660736 Genomic DNA. Translation: ABV74211.1 .
    EF660736 Genomic DNA. Translation: ABV74212.1 .
    EF660736 Genomic DNA. Translation: ABV74213.1 .
    EF660737 mRNA. Translation: ABV45396.1 .
    EF660738 mRNA. Translation: ABV45397.1 .
    EF660739 mRNA. Translation: ABV45398.1 .
    EF660740 mRNA. Translation: ABV45399.1 .
    EF660741 mRNA. Translation: ABV45400.1 .
    RefSeqi NP_001103778.1. NM_001110308.1. [A8BQB4-1 ]
    UniGenei Eca.16037.

    3D structure databases

    ProteinModelPortali A8BQB4.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9796.ENSECAP00000009911.

    Protein family/group databases

    CAZyi GH13. Glycoside Hydrolase Family 13.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 100050695.
    KEGGi ecb:100050695.

    Organism-specific databases

    CTDi 178.

    Phylogenomic databases

    eggNOGi COG3408.
    HOGENOMi HOG000212981.
    HOVERGENi HBG005824.
    InParanoidi A8BQB4.
    KOi K01196.

    Family and domain databases

    Gene3Di 3.20.20.80. 5 hits.
    InterProi IPR008928. 6-hairpin_glycosidase-like.
    IPR010401. AGL/Gdb1.
    IPR029436. AGL_euk_N.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR006421. Glycogen_debranch_met.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    PANTHERi PTHR10569. PTHR10569. 1 hit.
    Pfami PF06202. GDE_C. 1 hit.
    PF14699. hGDE_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48208. SSF48208. 2 hits.
    SSF51445. SSF51445. 2 hits.
    TIGRFAMsi TIGR01531. glyc_debranch. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Characterization of the equine glycogen debranching enzyme gene (AGL): Genomic and cDNA structure, localization, polymorphism and expression."
      Herszberg B., Mata X., Giulotto E., Decaunes P., Piras F.M., Chowdhary B.P., Chaffaux S., Guerin G.
      Gene 404:1-9(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS 1 AND 2), TISSUE SPECIFICITY, VARIANTS THR-326 AND LEU-368.

    Entry informationi

    Entry nameiGDE_HORSE
    AccessioniPrimary (citable) accession number: A8BQB4
    Secondary accession number(s): A8BQB7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 30, 2010
    Last sequence update: November 13, 2007
    Last modified: October 1, 2014
    This is version 43 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3