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A8BQB4

- GDE_HORSE

UniProt

A8BQB4 - GDE_HORSE

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Protein
Glycogen debranching enzyme
Gene
AGL
Organism
Equus caballus (Horse)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Multifunctional enzyme acting as 1,4-alpha-D-glucan:1,4-alpha-D-glucan 4-alpha-D-glycosyltransferase and amylo-1,6-glucosidase in glycogen degradation By similarity.By similarity

Catalytic activityi

Transfers a segment of a (1->4)-alpha-D-glucan to a new position in an acceptor, which may be glucose or a (1->4)-alpha-D-glucan.By similarity
Hydrolysis of (1->6)-alpha-D-glucosidic branch linkages in glycogen phosphorylase limit dextrin.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei527 – 5271 By similarityBy similarity
Active sitei530 – 5301 By similarityBy similarity
Active sitei628 – 6281 By similarityBy similarity

GO - Molecular functioni

  1. 4-alpha-glucanotransferase activity Source: UniProtKB-EC
  2. amylo-alpha-1,6-glucosidase activity Source: UniProtKB-EC

GO - Biological processi

  1. glycogen biosynthetic process Source: UniProtKB-KW
  2. glycogen catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Glycosyltransferase, Hydrolase, Transferase

Keywords - Biological processi

Glycogen biosynthesis

Protein family/group databases

CAZyiGH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycogen debranching enzyme
Alternative name(s):
Glycogen debrancher
Including the following 2 domains:
4-alpha-glucanotransferase (EC:2.4.1.25)
Alternative name(s):
Oligo-1,4-1,4-glucantransferase
Amylo-alpha-1,6-glucosidase (EC:3.2.1.33)
Short name:
Amylo-1,6-glucosidase
Alternative name(s):
Dextrin 6-alpha-D-glucosidase
Gene namesi
Name:AGL
OrganismiEquus caballus (Horse)
Taxonomic identifieri9796 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaPerissodactylaEquidaeEquus
ProteomesiUP000002281: Unplaced

Subcellular locationi

Cytoplasm By similarity
Note: Under glycogenolytic conditions localizes to the nucleus By similarity.By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 15331533Glycogen debranching enzyme
PRO_0000401163Add
BLAST

Post-translational modificationi

Ubiquitinated By similarity.By similarity

Keywords - PTMi

Ubl conjugation

Expressioni

Tissue specificityi

Ubiquitous. Expressed in striated skeletal muscle, heart, liver, spleen, skin, spinal cord, lung, kidney and testicle.1 Publication

Interactioni

Subunit structurei

Monomer. Interacts with NHLRC1/malin By similarity.By similarity

Protein-protein interaction databases

STRINGi9796.ENSECAP00000009911.

Structurei

3D structure databases

ProteinModelPortaliA8BQB4.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni? – 1533Amylo-1,6-glucosidaseBy similarity
Regioni1 – ?4-alpha-glucanotransferaseBy similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG3408.
HOGENOMiHOG000212981.
HOVERGENiHBG005824.
InParanoidiA8BQB4.
KOiK01196.

Family and domain databases

Gene3Di3.20.20.80. 5 hits.
InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR010401. AGL/Gdb1.
IPR029436. AGL_euk_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR006421. Glycogen_debranch_met.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10569. PTHR10569. 1 hit.
PfamiPF06202. GDE_C. 1 hit.
PF14699. hGDE_N. 1 hit.
[Graphical view]
SUPFAMiSSF48208. SSF48208. 2 hits.
SSF51445. SSF51445. 2 hits.
TIGRFAMsiTIGR01531. glyc_debranch. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 11 Publication (identifier: A8BQB4-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MGHSKQIRTL LLNEMEKLEK TLFRLEQGFE LQFRLGPTLQ GKAVTVYTNY     50
PFPGETFNRE KFHSLQWENP TEREDDSDKY CKLNLQQAGS FQYYFLQGNE 100
KSGGGYIVVD PILRVGADNH VLPLDCVTLQ TFLTKCLGPF DEWESRLRVA 150
KESGYNMIHF TPLQTLGLSR SSYSLADQLE LNPDFSRPNK KYTWHDVGQL 200
VEKLKKEWDI LCITDVVYNH TAANSKWIHE HPESAYNLVN SPHLKPAWVL 250
DRALWHLSCD VAEGKYREKG VPALIENDHQ MNCIRKIIWE DIYPKIHLWE 300
FFQVDVHKAV EQFRGLLTQE NRKIISQPDP KQHLKIIQDP EYRRLGCTVD 350
MNIALATFIP HDNGPAAIDE CCNWFRKRIE ELNAEKHQLV NYHQEQAVNC 400
LLGNVFYERL AGHGPKLGPV TRKHPLVTRY FTFPFEEMTP STEESMIHLP 450
NKACFLMAHN GWVMGDDPLR NFAEPGSDVY LRRELICWGD SVKLRYGNKP 500
EDCPYLWAHM KKYTEITATH FQGVRLDNCH STPIHVAEYM LDAARKLQPN 550
LYVVAELFTG SEDLDNIFVT RLGISSLIRE AMSAADSHEE GRLVYRYGGE 600
PVGSFVQPCL RPLMPAIAHA LFMDITHDNE CPIVHRSAYD ALPSSTIVSM 650
ASCASGSTKG YDELVPHQIS VVSEERFYTK WNPEALPSNT GEVNFQSGII 700
AARRAINKLH QELGAKGFIQ VYVDQVDQDI VAVTRHSPSI HQSVVSVSRT 750
AFRNPKTSFY SKEVPHMYIP GKIEEVVLEA RTIERHTIPY KKDENSINGM 800
PDITVEIREH IQLNESKIVK HAGIVTKGPN EFVQEIEFEN LTPGSVIIFR 850
VSLDPHAQVA VGILRNHLTQ FSPHFKSGSL AVDNADPILK IPFASIASKL 900
TLAELNQVLY RCESEEQEDG GGCYNIPNWS SLKYAGLQGL MSILAEIRPR 950
NDLGHPFCDN LRSGDWMIDY VSSRLISRSG TIAEVGKWLQ AMFLYLKQIP 1000
RYLIPCYFDA ILIGAYTTLL DIAWKQMSSF VQNGSTFVKH LSLGSVQMCG 1050
VGKFPSLPLL SPSLTDLPYR VNEITKEKEQ CCGSLAAGLP HFSAGIFRCW 1100
GRDTFIALRG LLLVTGRYLE ARNIILAFAG TLRHGLIPNL LGEGTHARYN 1150
CRDAVWWWLQ CIQDYCKIVP NGLDILRCPV SRMYPTDDSV PLSAGTVDQP 1200
LFEVIQEAMQ RHVQGIQFRE RNAGPQIDRN MKDEGFNITA GVDEETGFVY 1250
GGNRFNCGTW MDKMGESDRA RNRGIPATPR DGSAVEIVGL SKSAVRWLLE 1300
LSRKNIFPYH EVRVKRHGKF VTVSYDEWNR KIQDNFEKLF HVSEDPSDFN 1350
EKHPELVHKR GIYKDSYGAS SPWCDYQLRP NFTIAMVVAP ELFTPEKAWK 1400
ALEIAEKKLL GPLGMKTLDP DDMVYCGIYD NALDNDNYNL AKGFNYHQGP 1450
EWLWPTGYFL RAKLYFSKLM GPETNAKTMF LVKNVLSRHY VHLERSPWKG 1500
LPELTNENGQ YCPFSCETQA WSIATVLETL YDL 1533
Length:1,533
Mass (Da):174,666
Last modified:November 13, 2007 - v1
Checksum:iC19B5C7C192302F3
GO
Isoform 21 Publication (identifier: A8BQB4-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-156: Missing.

Note: No experimental confirmation available.

Show »
Length:1,377
Mass (Da):156,636
Checksum:iB2E718893457946E
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti326 – 3261S → T.1 Publication
Natural varianti368 – 3681I → L.1 Publication

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 156156Missing in isoform 2. 1 Publication
VSP_040070Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
EF660736 Genomic DNA. Translation: ABV45394.1.
EF660736 Genomic DNA. Translation: ABV45395.1.
EF660736 Genomic DNA. Translation: ABV74211.1.
EF660736 Genomic DNA. Translation: ABV74212.1.
EF660736 Genomic DNA. Translation: ABV74213.1.
EF660737 mRNA. Translation: ABV45396.1.
EF660738 mRNA. Translation: ABV45397.1.
EF660739 mRNA. Translation: ABV45398.1.
EF660740 mRNA. Translation: ABV45399.1.
EF660741 mRNA. Translation: ABV45400.1.
RefSeqiNP_001103778.1. NM_001110308.1. [A8BQB4-1]
UniGeneiEca.16037.

Genome annotation databases

GeneIDi100050695.
KEGGiecb:100050695.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
EF660736 Genomic DNA. Translation: ABV45394.1 .
EF660736 Genomic DNA. Translation: ABV45395.1 .
EF660736 Genomic DNA. Translation: ABV74211.1 .
EF660736 Genomic DNA. Translation: ABV74212.1 .
EF660736 Genomic DNA. Translation: ABV74213.1 .
EF660737 mRNA. Translation: ABV45396.1 .
EF660738 mRNA. Translation: ABV45397.1 .
EF660739 mRNA. Translation: ABV45398.1 .
EF660740 mRNA. Translation: ABV45399.1 .
EF660741 mRNA. Translation: ABV45400.1 .
RefSeqi NP_001103778.1. NM_001110308.1. [A8BQB4-1 ]
UniGenei Eca.16037.

3D structure databases

ProteinModelPortali A8BQB4.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9796.ENSECAP00000009911.

Protein family/group databases

CAZyi GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 100050695.
KEGGi ecb:100050695.

Organism-specific databases

CTDi 178.

Phylogenomic databases

eggNOGi COG3408.
HOGENOMi HOG000212981.
HOVERGENi HBG005824.
InParanoidi A8BQB4.
KOi K01196.

Family and domain databases

Gene3Di 3.20.20.80. 5 hits.
InterProi IPR008928. 6-hairpin_glycosidase-like.
IPR010401. AGL/Gdb1.
IPR029436. AGL_euk_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR006421. Glycogen_debranch_met.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
PANTHERi PTHR10569. PTHR10569. 1 hit.
Pfami PF06202. GDE_C. 1 hit.
PF14699. hGDE_N. 1 hit.
[Graphical view ]
SUPFAMi SSF48208. SSF48208. 2 hits.
SSF51445. SSF51445. 2 hits.
TIGRFAMsi TIGR01531. glyc_debranch. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Characterization of the equine glycogen debranching enzyme gene (AGL): Genomic and cDNA structure, localization, polymorphism and expression."
    Herszberg B., Mata X., Giulotto E., Decaunes P., Piras F.M., Chowdhary B.P., Chaffaux S., Guerin G.
    Gene 404:1-9(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS 1 AND 2), TISSUE SPECIFICITY, VARIANTS THR-326 AND LEU-368.

Entry informationi

Entry nameiGDE_HORSE
AccessioniPrimary (citable) accession number: A8BQB4
Secondary accession number(s): A8BQB7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 30, 2010
Last sequence update: November 13, 2007
Last modified: June 11, 2014
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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