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A8BQB4 (GDE_HORSE) Reviewed, UniProtKB/Swiss-Prot

Last modified December 11, 2013. Version 40. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glycogen debranching enzyme
Alternative name(s):
Glycogen debrancher

Including the following 2 domains:

  1. 4-alpha-glucanotransferase
    EC=2.4.1.25
    Alternative name(s):
    Oligo-1,4-1,4-glucantransferase
  2. Amylo-alpha-1,6-glucosidase
    Short name=Amylo-1,6-glucosidase
    EC=3.2.1.33
    Alternative name(s):
    Dextrin 6-alpha-D-glucosidase
Gene names
Name:AGL
OrganismEquus caballus (Horse) [Reference proteome]
Taxonomic identifier9796 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaPerissodactylaEquidaeEquus

Protein attributes

Sequence length1533 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Multifunctional enzyme acting as 1,4-alpha-D-glucan:1,4-alpha-D-glucan 4-alpha-D-glycosyltransferase and amylo-1,6-glucosidase in glycogen degradation By similarity. UniProtKB P35573

Catalytic activity

Transfers a segment of a (1->4)-alpha-D-glucan to a new position in an acceptor, which may be glucose or a (1->4)-alpha-D-glucan. UniProtKB P35573

Hydrolysis of (1->6)-alpha-D-glucosidic branch linkages in glycogen phosphorylase limit dextrin. UniProtKB P35573

Subunit structure

Monomer. Interacts with NHLRC1/malin By similarity. UniProtKB P35573

Subcellular location

Cytoplasm By similarity. Note: Under glycogenolytic conditions localizes to the nucleus By similarity. UniProtKB P35573

Tissue specificity

Ubiquitous. Expressed in striated skeletal muscle, heart, liver, spleen, skin, spinal cord, lung, kidney and testicle. Ref.1

Post-translational modification

Ubiquitinated By similarity. UniProtKB P35573

Sequence similarities

Belongs to the glycogen debranching enzyme family.

Ontologies

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 Ref.1 (identifier: A8BQB4-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 Ref.1 (identifier: A8BQB4-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-156: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 15331533Glycogen debranching enzyme
PRO_0000401163

Regions

Region1 – ?4-alpha-glucanotransferase UniProtKB P35573
Region? – 1533Amylo-1,6-glucosidase UniProtKB P35573

Sites

Active site5271 By similarity UniProtKB P35573
Active site5301 By similarity UniProtKB P35573
Active site6281 By similarity UniProtKB P35573

Natural variations

Alternative sequence1 – 156156Missing in isoform 2. Ref.1
VSP_040070
Natural variant3261S → T. Ref.1
Natural variant3681I → L. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified November 13, 2007. Version 1.
Checksum: C19B5C7C192302F3

FASTA1,533174,666
        10         20         30         40         50         60 
MGHSKQIRTL LLNEMEKLEK TLFRLEQGFE LQFRLGPTLQ GKAVTVYTNY PFPGETFNRE 

        70         80         90        100        110        120 
KFHSLQWENP TEREDDSDKY CKLNLQQAGS FQYYFLQGNE KSGGGYIVVD PILRVGADNH 

       130        140        150        160        170        180 
VLPLDCVTLQ TFLTKCLGPF DEWESRLRVA KESGYNMIHF TPLQTLGLSR SSYSLADQLE 

       190        200        210        220        230        240 
LNPDFSRPNK KYTWHDVGQL VEKLKKEWDI LCITDVVYNH TAANSKWIHE HPESAYNLVN 

       250        260        270        280        290        300 
SPHLKPAWVL DRALWHLSCD VAEGKYREKG VPALIENDHQ MNCIRKIIWE DIYPKIHLWE 

       310        320        330        340        350        360 
FFQVDVHKAV EQFRGLLTQE NRKIISQPDP KQHLKIIQDP EYRRLGCTVD MNIALATFIP 

       370        380        390        400        410        420 
HDNGPAAIDE CCNWFRKRIE ELNAEKHQLV NYHQEQAVNC LLGNVFYERL AGHGPKLGPV 

       430        440        450        460        470        480 
TRKHPLVTRY FTFPFEEMTP STEESMIHLP NKACFLMAHN GWVMGDDPLR NFAEPGSDVY 

       490        500        510        520        530        540 
LRRELICWGD SVKLRYGNKP EDCPYLWAHM KKYTEITATH FQGVRLDNCH STPIHVAEYM 

       550        560        570        580        590        600 
LDAARKLQPN LYVVAELFTG SEDLDNIFVT RLGISSLIRE AMSAADSHEE GRLVYRYGGE 

       610        620        630        640        650        660 
PVGSFVQPCL RPLMPAIAHA LFMDITHDNE CPIVHRSAYD ALPSSTIVSM ASCASGSTKG 

       670        680        690        700        710        720 
YDELVPHQIS VVSEERFYTK WNPEALPSNT GEVNFQSGII AARRAINKLH QELGAKGFIQ 

       730        740        750        760        770        780 
VYVDQVDQDI VAVTRHSPSI HQSVVSVSRT AFRNPKTSFY SKEVPHMYIP GKIEEVVLEA 

       790        800        810        820        830        840 
RTIERHTIPY KKDENSINGM PDITVEIREH IQLNESKIVK HAGIVTKGPN EFVQEIEFEN 

       850        860        870        880        890        900 
LTPGSVIIFR VSLDPHAQVA VGILRNHLTQ FSPHFKSGSL AVDNADPILK IPFASIASKL 

       910        920        930        940        950        960 
TLAELNQVLY RCESEEQEDG GGCYNIPNWS SLKYAGLQGL MSILAEIRPR NDLGHPFCDN 

       970        980        990       1000       1010       1020 
LRSGDWMIDY VSSRLISRSG TIAEVGKWLQ AMFLYLKQIP RYLIPCYFDA ILIGAYTTLL 

      1030       1040       1050       1060       1070       1080 
DIAWKQMSSF VQNGSTFVKH LSLGSVQMCG VGKFPSLPLL SPSLTDLPYR VNEITKEKEQ 

      1090       1100       1110       1120       1130       1140 
CCGSLAAGLP HFSAGIFRCW GRDTFIALRG LLLVTGRYLE ARNIILAFAG TLRHGLIPNL 

      1150       1160       1170       1180       1190       1200 
LGEGTHARYN CRDAVWWWLQ CIQDYCKIVP NGLDILRCPV SRMYPTDDSV PLSAGTVDQP 

      1210       1220       1230       1240       1250       1260 
LFEVIQEAMQ RHVQGIQFRE RNAGPQIDRN MKDEGFNITA GVDEETGFVY GGNRFNCGTW 

      1270       1280       1290       1300       1310       1320 
MDKMGESDRA RNRGIPATPR DGSAVEIVGL SKSAVRWLLE LSRKNIFPYH EVRVKRHGKF 

      1330       1340       1350       1360       1370       1380 
VTVSYDEWNR KIQDNFEKLF HVSEDPSDFN EKHPELVHKR GIYKDSYGAS SPWCDYQLRP 

      1390       1400       1410       1420       1430       1440 
NFTIAMVVAP ELFTPEKAWK ALEIAEKKLL GPLGMKTLDP DDMVYCGIYD NALDNDNYNL 

      1450       1460       1470       1480       1490       1500 
AKGFNYHQGP EWLWPTGYFL RAKLYFSKLM GPETNAKTMF LVKNVLSRHY VHLERSPWKG 

      1510       1520       1530 
LPELTNENGQ YCPFSCETQA WSIATVLETL YDL 

« Hide

Isoform 2 [UniParc].

Checksum: B2E718893457946E
Show »

FASTA1,377156,636

References

[1]"Characterization of the equine glycogen debranching enzyme gene (AGL): Genomic and cDNA structure, localization, polymorphism and expression."
Herszberg B., Mata X., Giulotto E., Decaunes P., Piras F.M., Chowdhary B.P., Chaffaux S., Guerin G.
Gene 404:1-9(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS 1 AND 2), TISSUE SPECIFICITY, VARIANTS THR-326 AND LEU-368.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
EF660736 Genomic DNA. Translation: ABV45394.1.
EF660736 Genomic DNA. Translation: ABV45395.1.
EF660736 Genomic DNA. Translation: ABV74211.1.
EF660736 Genomic DNA. Translation: ABV74212.1.
EF660736 Genomic DNA. Translation: ABV74213.1.
EF660737 mRNA. Translation: ABV45396.1.
EF660738 mRNA. Translation: ABV45397.1.
EF660739 mRNA. Translation: ABV45398.1.
EF660740 mRNA. Translation: ABV45399.1.
EF660741 mRNA. Translation: ABV45400.1.
RefSeqNP_001103778.1. NM_001110308.1.
UniGeneEca.16037.

3D structure databases

ProteinModelPortalA8BQB4.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9796.ENSECAP00000009911.

Protein family/group databases

CAZyGH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID100050695.
KEGGecb:100050695.

Organism-specific databases

CTD178.

Phylogenomic databases

eggNOGCOG3408.
HOGENOMHOG000212981.
HOVERGENHBG005824.
InParanoidA8BQB4.
KOK01196.

Family and domain databases

Gene3D3.20.20.80. 5 hits.
InterProIPR008928. 6-hairpin_glycosidase-like.
IPR010401. AGL/Gdb1.
IPR013781. Glyco_hydro_catalytic_dom.
IPR006421. Glycogen_debranch_met.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERPTHR10569. PTHR10569. 1 hit.
PfamPF06202. GDE_C. 1 hit.
[Graphical view]
SUPFAMSSF48208. SSF48208. 2 hits.
SSF51445. SSF51445. 2 hits.
TIGRFAMsTIGR01531. glyc_debranch. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGDE_HORSE
AccessionPrimary (citable) accession number: A8BQB4
Secondary accession number(s): A8BQB7
Entry history
Integrated into UniProtKB/Swiss-Prot: November 30, 2010
Last sequence update: November 13, 2007
Last modified: December 11, 2013
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families