A8AZA7 (SYP_STRGC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Proline--tRNA ligase
EC=6.1.1.15

Alternative name(s):
Prolyl-tRNA synthetase
Short name=ProRS

Gene names

Name:	proS
Ordered Locus Names:	SGO_1851

Organism

Streptococcus gordonii (strain Challis / ATCC 35105 / CH1 / DL1 / V288) [Complete proteome] [HAMAP]

Taxonomic identifier

29390 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Lactobacillales › Streptococcaceae › Streptococcus › Streptococcus gordonii

Protein attributes

Sequence length	616 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids such as alanine and cysteine, to avoid such errors it has two additional distinct editing activities against alanine. One activity is designated as 'pretransfer' editing and involves the tRNA(Pro)-independent hydrolysis of activated Ala-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Ala-tRNA(Pro). The misacylated Cys-tRNA(Pro) is not edited by ProRS By similarity. HAMAP-Rule MF_01569
Catalytic activity	ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro). HAMAP-Rule MF_01569
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Domain	Consists of three domains: the N-terminal catalytic domain, the editing domain and the C-terminal anticodon-binding domain By similarity. HAMAP-Rule MF_01569
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	prolyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP regulation of translational fidelity Inferred from electronic annotation. Source: GOC
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP aminoacyl-tRNA editing activity Inferred from electronic annotation. Source: InterPro proline-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 616	616	Proline--tRNA ligase HAMAP-Rule MF_01569		PRO_1000087859

Sequences

	Sequence		Length	Mass (Da)
	A8AZA7 [UniParc]. Last modified October 23, 2007. Version 1. Checksum: 1CE99E45D366006B	FASTA	616	68,509
	10 20 30 40 50 60 MKQSKMLIPT LREMPSDAQV ISHALLLRAG YVRQVSAGVY SYLPLANRVI EKAKNIMRQE 70 80 90 100 110 120 FEKIGAVEML APALLSADLW RESGRYETYG DDLFKLKNRE GSDFILGPTH EETFTALVRD 130 140 150 160 170 180 SVKSYKQLPL NLYQIQPKYR DEKRPRNGLL RTREFIMKDA YSFHANYDSL DVTYDEYKSA 190 200 210 220 230 240 YEKIFTRSEI DFKAIIGDGG AMGGKDSQEF MAITPDRTDL DRWLVLDKSV ASLDEIPADV 250 260 270 280 290 300 LEAIKAELSN WMVSGEDTIA YSSESSYAAN LEMATNEYKP SNRVVAETEL VRVETPNCKT 310 320 330 340 350 360 IDEVAAFLQV SEEQTIKTLV YIADEKPVVA LLVGNDQLNE VKLKNHLGAD FFEAATEAEV 370 380 390 400 410 420 QELFGANFGS LGPVNLPEEV TIIADRKVQD LSNAVAGANE DGYHLTGVNP GRDFTAEYVD 430 440 450 460 470 480 IREVREGEIS PDGNGVLKFA RGIEIGHIFK LGTRYSDSMN ATVLDENGRA VPLVMGCYGI 490 500 510 520 530 540 GVSRLLSAVM EQHARLFVNK TPKGEYRYAW GINFPKELAP FDVHLIPVNV KDEESLALTD 550 560 570 580 590 600 KIEESLVGAG YEVLVDDRNE RVGVKFSDSD LIGLPIRVTV GKKAAEGIVE VKIKASGDTI 610 EVHADNLIET LSILTK

References

[1]

"Genome-wide transcriptional changes in Streptococcus gordonii in response to competence signaling peptide." Vickerman M.M., Iobst S., Jesionowski A.M., Gill S.R. J. Bacteriol. 189:7799-7807(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Challis / ATCC 35105 / CH1 / DL1 / V288.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000725 Genomic DNA. Translation: ABV10446.1.
RefSeq	YP_001451117.1. NC_009785.1.
3D structure databases
ProteinModelPortal	A8AZA7.
SMR	A8AZA7. Positions 1-614.
ModBase	Search...
Protein-protein interaction databases
STRING	467705.SGO_1851.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABV10446; ABV10446; SGO_1851.
GeneID	5599745.
KEGG	sgo:SGO_1851.
PATRIC	19661650. VBIStrGor124371_1818.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0442.
HOGENOM	HOG000076893.
KO	K01881.
OMA	IQPAELW.
ProtClustDB	PRK09194.
Enzyme and pathway databases
BioCyc	SGOR467705:GH3R-1893-MONOMER.
Family and domain databases
Gene3D	3.40.50.800. 1 hit. 3.90.960.10. 1 hit.
HAMAP	MF_01569. Pro_tRNA_synth_type1.
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-dom. IPR006195. aa-tRNA-synth_II. IPR004154. Anticodon-bd. IPR002316. Pro-tRNA-ligase_IIa. IPR004500. Pro-tRNA-synth_IIa_bac-type. IPR023717. Pro-tRNA-Synthase_IIa_type1. IPR007214. YbaK/aa-tRNA-synth-assoc-dom. [Graphical view]
PANTHER	PTHR11451:SF3. PTHR11451:SF3. 1 hit.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF00587. tRNA-synt_2b. 1 hit. PF04073. YbaK. 1 hit. [Graphical view]
PRINTS	PR01046. TRNASYNTHPRO.
SUPFAM	SSF52954. Anticodon_bd. 1 hit. SSF55826. YbaK/aa-tRNA-synth-assoc-reg. 1 hit.
TIGRFAMs	TIGR00409. proS_fam_II. 2 hits.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYP_STRGC

Accession

Primary (citable) accession number: A8AZA7

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	October 23, 2007
Last modified:	May 1, 2013
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program