Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

A8AY66 (DDL_STRGC) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 48. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase
Gene names
Name:ddl
Ordered Locus Names:SGO_1447
OrganismStreptococcus gordonii (strain Challis / ATCC 35105 / CH1 / DL1 / V288) [Complete proteome] [HAMAP]
Taxonomic identifier29390 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus

Protein attributes

Sequence length348 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 348348D-alanine--D-alanine ligase HAMAP-Rule MF_00047
PRO_1000074800

Regions

Domain132 – 334203ATP-grasp
Nucleotide binding162 – 21756ATP By similarity

Sites

Metal binding2881Magnesium or manganese 1 By similarity
Metal binding3011Magnesium or manganese 1 By similarity
Metal binding3011Magnesium or manganese 2 By similarity
Metal binding3031Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
A8AY66 [UniParc].

Last modified October 23, 2007. Version 1.
Checksum: C702496CEA815811

FASTA34838,643
        10         20         30         40         50         60 
MAKKSLILLY GGRSAEREVS VLSAESVMRA INYDLFSVKT YFITKEGDFI KTQEFSSKPA 

        70         80         90        100        110        120 
EDVRLMTNDT VDMSRKIKPS DIYEEGAVVF PVLHGPMGED GSIQGFLEVL KMPYVGCNIL 

       130        140        150        160        170        180 
SSSLAMDKIT TKRVLESAGI AQVPYVALVD GEDLEQKIQE IEEKLSYPVF TKPSNMGSSV 

       190        200        210        220        230        240 
GISKSDNQEE LRASLDLAFK YDSRVLVEQG VTAREIEVGL LGNADVKSSL PGEVVKDVAF 

       250        260        270        280        290        300 
YDYQAKYIDN KITMAIPAQL PEGVVNTMRQ NAEIAFRAIG GLGLSRCDFF YTEDGQVFLN 

       310        320        330        340 
ELNTMPGFTQ WSMYPLLWEN MGLAYPDLIE KLVALAEEAF AKREAHLL 

« Hide

References

[1]"Genome-wide transcriptional changes in Streptococcus gordonii in response to competence signaling peptide."
Vickerman M.M., Iobst S., Jesionowski A.M., Gill S.R.
J. Bacteriol. 189:7799-7807(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Challis / ATCC 35105 / CH1 / DL1 / V288.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000725 Genomic DNA. Translation: ABV09351.1.
RefSeqYP_001450726.1. NC_009785.1.

3D structure databases

ProteinModelPortalA8AY66.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING467705.SGO_1447.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABV09351; ABV09351; SGO_1447.
GeneID5598634.
KEGGsgo:SGO_1447.
PATRIC19660846. VBIStrGor124371_1424.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011593.
KOK01921.
OMAMDKIAMK.
OrthoDBEOG64BQ73.
ProtClustDBPRK01966.

Enzyme and pathway databases

BioCycSGOR467705:GH3R-1446-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDL_STRGC
AccessionPrimary (citable) accession number: A8AY66
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: October 23, 2007
Last modified: February 19, 2014
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways