A8AXH4 (A8AXH4_STRGC) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Submitted name:
DNA topoisomerase I EMBL ABV10348.1
EC=5.99.1.2 EMBL ABV10348.1

Gene names

Name:	topA EMBL ABV10348.1
Ordered Locus Names:	SGO_1197

Organism

Streptococcus gordonii (strain Challis / ATCC 35105 / CH1 / DL1 / V288) [Complete proteome] [HAMAP]

Taxonomic identifier

29390 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Streptococcus

Protein attributes

Sequence length	697 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. SAAS SAAS000380
Catalytic activity	ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. SAAS SAAS005733
Sequence similarities	Contains 1 Toprim domain. SAAS SAAS023406

Ontologies

Keywords
Ligand	ATP-binding SAAS SAAS005733 DNA-binding SAAS SAAS005733 Nucleotide-binding
Molecular function	Isomerase Topoisomerase SAAS SAAS005733
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase type I activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	A8AXH4 [UniParc]. Last modified October 23, 2007. Version 1. Checksum: 26FF174C69AC13E3	FASTA	697	79,503
	10 20 30 40 50 60 MYKLVTKTSK KSTTKKNLVI VESPAKAKTI EKYLGRNYKV LASVGHIRDL KKSTMSIDFE 70 80 90 100 110 120 NNYEPQYINI RGKGPLINDL RKEAKKAKQV FLASDPDREG EAISWHLAHI LNLDEKEKNR 130 140 150 160 170 180 VVFNEITKDA VKNAFKEPRQ INMDLVDAQQ ARRVLDRLVG YSISPILWKK VKKGLSAGRV 190 200 210 220 230 240 QSVALKLIID RENEINAFKP EEYWTIDSTF KKGSRQFQAS FYGINGRKMK LTNNDEVKEV 250 260 270 280 290 300 LSLLKGDDFK VESVERKERR RNAPLPYTTS SMQQDAANKI NFRTRKTMMV AQQLYEGINI 310 320 330 340 350 360 GSGVQGLITY MRTDSTRISP VAQNEAANYI TDRFGAKYSK HGSKIKNASG AQDAHEAIRP 370 380 390 400 410 420 SSVFNTPESI AKYLDKDQLK LYTLIWNRFV ASQMTAAVFD TMNVKIEQNG VQFTANGSQV 430 440 450 460 470 480 KFDGYLAIYN DSDKNKMLPD MEKGDTVKRV NTNPEQHFTQ PPARYSEATL IKTLEENGVG 490 500 510 520 530 540 RPSTYAPTIE TIQKRYYVKL VSKRFEPTEL GEIVNSLIVE FFPGIVNVKF TAEMEAKLDD 550 560 570 580 590 600 VEVGKEQWQK VIDEFYRPFE KEVAKAESEM EKIQIKDEPA GFDCEVCGSP MVIKLGRFGK 610 620 630 640 650 660 FYACSNFPDC RHTQAIVKEI GVTCPQCQKG HVIERKTKRN RIFYGCNRYP DCDFTSWDKP 670 680 690 VGRNCPKCDH YLVEKKVRGG GKQVVCSNGD YEEEKVK

References

[1]	"Genome-wide transcriptional changes in Streptococcus gordonii in response to competence signaling peptide." Vickerman M.M., Iobst S., Jesionowski A.M., Gill S.R. J. Bacteriol. 189:7799-7807(2007) [PubMed: 17720781] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: Challis EMBL ABV10348.1.
[2]	Gill S. Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: Challis EMBL ABV10348.1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000725 Genomic DNA. Translation: ABV10348.1.
RefSeq	YP_001450484.1. NC_009785.1.
3D structure databases
ProteinModelPortal	A8AXH4.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBSTRT00000010782; EBSTRP00000010339; EBSTRG00000010782.
GeneID	5599500.
GenomeReviews	Gene locus SGO_1197 in contig CP000725_GR.
KEGG	sgo:SGO_1197.
PATRIC	19660347. VBIStrGor124371_1180.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000027862.
HOGENOM	HBG512935.
OMA	PREIDIK.
ProtClustDB	PRK05582.
Enzyme and pathway databases
BioCyc	SGOR29390:SGO_1197-MONOMER.
Family and domain databases
InterPro	IPR000380. Topo_IA. IPR003601. Topo_IA_2. IPR023406. Topo_IA_AS. IPR013497. Topo_IA_cen. IPR013824. Topo_IA_cen_sub1. IPR013825. Topo_IA_cen_sub2. IPR023405. Topo_IA_core_domain. IPR003602. Topo_IA_DNA-bd. IPR013498. Topo_IA_Znf. IPR005733. TopoI_bac-type. IPR006171. Toprim_domain. [Graphical view]
Gene3D	G3DSA:1.10.460.10. Topo_IA_cen_sub1. 2 hits. G3DSA:2.70.20.10. Topo_IA_cen_sub2. 2 hits.
KO	K03168.
PANTHER	PTHR11390. Topo_IA. 1 hit.
Pfam	PF01131. Topoisom_bac. 1 hit. PF01751. Toprim. 1 hit. PF01396. zf-C4_Topoisom. 2 hits. [Graphical view]
PRINTS	PR00417. PRTPISMRASEI.
SMART	SM00437. TOP1Ac. 1 hit. SM00436. TOP1Bc. 1 hit. SM00493. TOPRIM. 1 hit. [Graphical view]
SUPFAM	SSF56712. Topo_IA_core. 1 hit.
TIGRFAMs	TIGR01051. TopA_bact. 1 hit.
PROSITE	PS00396. TOPOISOMERASE_I_PROK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

A8AXH4_STRGC

Accession

Primary (citable) accession number: A8AXH4

Entry history

Integrated into UniProtKB/TrEMBL:	October 23, 2007
Last sequence update:	October 23, 2007
Last modified:	January 25, 2012
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)