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Protein

Glutamate 5-kinase

Gene

proB

Organism
Streptococcus gordonii (strain Challis / ATCC 35105 / CH1 / DL1 / V288)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the transfer of a phosphate group to glutamate to form L-glutamate 5-phosphate.UniRule annotation

Catalytic activityi

ATP + L-glutamate = ADP + L-glutamate 5-phosphate.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei9 – 91ATPUniRule annotation
Binding sitei49 – 491SubstrateUniRule annotation
Binding sitei136 – 1361SubstrateUniRule annotation
Binding sitei148 – 1481Substrate; via amide nitrogenUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi168 – 1692ATPUniRule annotation
Nucleotide bindingi210 – 2167ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. glutamate 5-kinase activity Source: UniProtKB-HAMAP
  3. RNA binding Source: InterPro

GO - Biological processi

  1. L-proline biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Proline biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciSGOR467705:GH3R-1095-MONOMER.
UniPathwayiUPA00098; UER00359.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate 5-kinaseUniRule annotation (EC:2.7.2.11UniRule annotation)
Alternative name(s):
Gamma-glutamyl kinaseUniRule annotation
Short name:
GKUniRule annotation
Gene namesi
Name:proBUniRule annotation
Ordered Locus Names:SGO_1097
OrganismiStreptococcus gordonii (strain Challis / ATCC 35105 / CH1 / DL1 / V288)
Taxonomic identifieri29390 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
ProteomesiUP000001131 Componenti: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 369369Glutamate 5-kinasePRO_1000081110Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi467705.SGO_1097.

Structurei

3D structure databases

ProteinModelPortaliA8AX76.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini275 – 35581PUAUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the glutamate 5-kinase family.UniRule annotation
Contains 1 PUA domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG0263.
HOGENOMiHOG000246369.
KOiK00931.
OMAiVIAWGRE.
OrthoDBiEOG6PGK7G.

Family and domain databases

Gene3Di2.30.130.10. 1 hit.
3.40.1160.10. 1 hit.
HAMAPiMF_00456. ProB.
InterProiIPR001048. Asp/Glu/Uridylate_kinase.
IPR001057. Glu/AcGlu_kinase.
IPR011529. Glu_5kinase.
IPR005715. Glu_5kinase/COase_Synthase.
IPR019797. Glutamate_5-kinase_CS.
IPR002478. PUA.
IPR015947. PUA-like_domain.
[Graphical view]
PfamiPF00696. AA_kinase. 1 hit.
PF01472. PUA. 1 hit.
[Graphical view]
PIRSFiPIRSF000729. GK. 1 hit.
PRINTSiPR00474. GLU5KINASE.
SMARTiSM00359. PUA. 1 hit.
[Graphical view]
SUPFAMiSSF53633. SSF53633. 1 hit.
SSF88697. SSF88697. 1 hit.
TIGRFAMsiTIGR01027. proB. 1 hit.
PROSITEiPS00902. GLUTAMATE_5_KINASE. 1 hit.
PS50890. PUA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A8AX76-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKAKRIVFKV GTSSLTNADG SLSRAKVKEI TRQLALLHEA GHELILVSSG
60 70 80 90 100
AIAAGFSSLG FKKRPTKVVD KQASAAVGQG LLLEEYTTNL LLKQIISAQI
110 120 130 140 150
LLTQDDFADK RRYKNAHQAL SVLLNRGAIP VINENDSVAI EELKVGDNDT
160 170 180 190 200
LSAQVAAMVQ ADLLVLLTDV DGLYTANPST NPDARRLEKI EKISSELIDI
210 220 230 240 250
AGGAGTSNGT GGMLTKIKAA TLATMSGVPV YICSSLKTDA LLEAAEETKD
260 270 280 290 300
GSLFLAQEKG LKTQKQWLAF YAKSQGEIYV DQGAADALRN KGKSLLVSGL
310 320 330 340 350
VSVSGSFAYQ DTVTVYEEGS GAILGKGRVR FGELALKDML KSNKPKGVVI
360
HRDDWISLTP ELNDLFAEF
Length:369
Mass (Da):39,471
Last modified:October 22, 2007 - v1
Checksum:i4A88199E06E6B279
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000725 Genomic DNA. Translation: ABV10021.1.
RefSeqiYP_001450386.1. NC_009785.1.

Genome annotation databases

EnsemblBacteriaiABV10021; ABV10021; SGO_1097.
KEGGisgo:SGO_1097.
PATRICi19660149. VBIStrGor124371_1081.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000725 Genomic DNA. Translation: ABV10021.1.
RefSeqiYP_001450386.1. NC_009785.1.

3D structure databases

ProteinModelPortaliA8AX76.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi467705.SGO_1097.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABV10021; ABV10021; SGO_1097.
KEGGisgo:SGO_1097.
PATRICi19660149. VBIStrGor124371_1081.

Phylogenomic databases

eggNOGiCOG0263.
HOGENOMiHOG000246369.
KOiK00931.
OMAiVIAWGRE.
OrthoDBiEOG6PGK7G.

Enzyme and pathway databases

UniPathwayiUPA00098; UER00359.
BioCyciSGOR467705:GH3R-1095-MONOMER.

Family and domain databases

Gene3Di2.30.130.10. 1 hit.
3.40.1160.10. 1 hit.
HAMAPiMF_00456. ProB.
InterProiIPR001048. Asp/Glu/Uridylate_kinase.
IPR001057. Glu/AcGlu_kinase.
IPR011529. Glu_5kinase.
IPR005715. Glu_5kinase/COase_Synthase.
IPR019797. Glutamate_5-kinase_CS.
IPR002478. PUA.
IPR015947. PUA-like_domain.
[Graphical view]
PfamiPF00696. AA_kinase. 1 hit.
PF01472. PUA. 1 hit.
[Graphical view]
PIRSFiPIRSF000729. GK. 1 hit.
PRINTSiPR00474. GLU5KINASE.
SMARTiSM00359. PUA. 1 hit.
[Graphical view]
SUPFAMiSSF53633. SSF53633. 1 hit.
SSF88697. SSF88697. 1 hit.
TIGRFAMsiTIGR01027. proB. 1 hit.
PROSITEiPS00902. GLUTAMATE_5_KINASE. 1 hit.
PS50890. PUA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genome-wide transcriptional changes in Streptococcus gordonii in response to competence signaling peptide."
    Vickerman M.M., Iobst S., Jesionowski A.M., Gill S.R.
    J. Bacteriol. 189:7799-7807(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Challis / ATCC 35105 / CH1 / DL1 / V288.

Entry informationi

Entry nameiPROB_STRGC
AccessioniPrimary (citable) accession number: A8AX76
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 19, 2008
Last sequence update: October 22, 2007
Last modified: March 31, 2015
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.