A8AVT9 (NADE_STRGC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NH(3)-dependent NAD(+) synthetase
EC=6.3.1.5

Gene names

Name:	nadE
Ordered Locus Names:	SGO_0583

Organism

Streptococcus gordonii (strain Challis / ATCC 35105 / CH1 / DL1 / V288) [Complete proteome] [HAMAP]

Taxonomic identifier

29390 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Streptococcus

Protein attributes

Sequence length	274 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+. HAMAP MF_00193
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1. HAMAP MF_00193
Sequence similarities	Belongs to the NAD synthetase family.

Ontologies

Keywords
Ligand	ATP-binding NAD Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW NAD+ synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro NAD+ synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 274	274	NH(3)-dependent NAD(+) synthetase HAMAP MF_00193		PRO_1000077624
Regions
	Nucleotide binding	46 – 53	8	ATP By similarity
Sites
	Active site	48	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A8AVT9 [UniParc]. Last modified October 23, 2007. Version 1. Checksum: 4F7BF980FB94D96A	FASTA	274	30,266
	10 20 30 40 50 60 MSLQEEIIAQ LGVKPIIDPE EEIRKSVDFL KAYLRKHPFL KSYVLGISGG QDSTLAGRLA 70 80 90 100 110 120 QLAVEEMRAE TRDDSYRFIA VRLPYGVQAD EDDAQKALTF IQPDVSLTVN IKESADAMTR 130 140 150 160 170 180 AVEATGAKVS DFNKGNIKAR SRMIAQYALA GSYSGAVIGT DHAAENVTAF FTKFGDGGAD 190 200 210 220 230 240 ILPLYRLNKR QGKQLLAALG ADPALYEKVP TADLEEEKPG IADEVALGVT YNEIDDYLEG 250 260 270 KSVSAQAQET IESWWHKGQH KRHLPITIFD EFWK

References

[1]

"Genome-wide transcriptional changes in Streptococcus gordonii in response to competence signaling peptide." Vickerman M.M., Iobst S., Jesionowski A.M., Gill S.R. J. Bacteriol. 189:7799-7807(2007) [PubMed: 17720781] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Challis / ATCC 35105 / CH1 / DL1 / V288.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000725 Genomic DNA. Translation: ABV10367.1.
RefSeq	YP_001449899.1. NC_009785.1.
3D structure databases
ProteinModelPortal	A8AVT9.
SMR	A8AVT9. Positions 2-274.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBSTRT00000011151; EBSTRP00000010708; EBSTRG00000011151.
GeneID	5598183.
GenomeReviews	Gene locus SGO_0583 in contig CP000725_GR.
KEGG	sgo:SGO_0583.
PATRIC	19659123. VBIStrGor124371_0582.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000027750.
HOGENOM	HBG351567.
OMA	IAQYEIA.
ProtClustDB	PRK00768.
Enzyme and pathway databases
BioCyc	SGOR29390:SGO_0583-MONOMER.
Family and domain databases
HAMAP	MF_00193. NadE. [Tree]
InterPro	IPR022310. NAD/GMP_synthase. IPR003694. NAD_synthase. IPR022926. NH(3)-dep_NAD(+)_synth. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01916.
Pfam	PF02540. NAD_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00552. NadE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NADE_STRGC

Accession

Primary (citable) accession number: A8AVT9

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	October 23, 2007
Last modified:	January 25, 2012
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program