A8AUN5 (SYE_STRGC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate--tRNA ligase
EC=6.1.1.17

Alternative name(s):
Glutamyl-tRNA synthetase
Short name=GluRS

Gene names

Name:	gltX
Ordered Locus Names:	SGO_0174

Organism

Streptococcus gordonii (strain Challis / ATCC 35105 / CH1 / DL1 / V288) [Complete proteome] [HAMAP]

Taxonomic identifier

29390 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Streptococcus

Protein attributes

Sequence length	485 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP MF_00022_B
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022_B
Subunit structure	Monomer By similarity. HAMAP MF_00022_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00022_B.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 485	485	Glutamate--tRNA ligase HAMAP MF_00022_B		PRO_1000074339
Regions
	Motif	11 – 21	11	"HIGH" region HAMAP MF_00022_B
	Motif	255 – 259	5	"KMSKS" region HAMAP MF_00022_B
Sites
	Binding site	258	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A8AUN5 [UniParc]. Last modified October 23, 2007. Version 1. Checksum: 3023945430AEB20A	FASTA	485	55,790
	10 20 30 40 50 60 MAKAIRVRYA PSPTGLLHIG NARTALFNYL YARHHGGTFI IRIEDTDRKR HVEDGERSQL 70 80 90 100 110 120 ENLRWLGMDW DESPETHENY RQSERLELYQ KYIDQLLAEG KAYKSYVTEE ELAAERERQE 130 140 150 160 170 180 AAGETPRYIN EYLGMSEEEK AAYIAEREAA GIIPTVRLSV NESGIYKWHD IVKGDIEFEG 190 200 210 220 230 240 GNIGGDWVIQ KKDGYPTYNF AVVIDDHDMQ ISHVIRGDDH IANTPKQLMV YEALGWEAPE 250 260 270 280 290 300 FGHMTLIINS ETGKKLSKRD TNTLQFIEDY RKKGYLPEAV FNFIALLGWN PGGEDEIFSR 310 320 330 340 350 360 EELIKLFDEN RLSKSPAAFD QKKLDWMSNE YIKNADFERI FALAKPYLEE AGRLTDKAEK 370 380 390 400 410 420 LVELYKPQMK SVDEIVPLTD LFFSDFPELT EAEREVMAGE TVPVVLEAFK AKLEAMTDEE 430 440 450 460 470 480 FVTENIFPQI KAVQKETGIK GKNLFMPIRI AVSGEMHGPE LPDTIYLLGR EKSIQHIENM LNQIQ

References

[1]

"Genome-wide transcriptional changes in Streptococcus gordonii in response to competence signaling peptide." Vickerman M.M., Iobst S., Jesionowski A.M., Gill S.R. J. Bacteriol. 189:7799-7807(2007) [PubMed: 17720781] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Challis / ATCC 35105 / CH1 / DL1 / V288.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000725 Genomic DNA. Translation: ABV10390.1.
RefSeq	YP_001449495.1. NC_009785.1.
3D structure databases
ProteinModelPortal	A8AUN5.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBSTRT00000011586; EBSTRP00000011143; EBSTRG00000011586.
GeneID	5597962.
GenomeReviews	Gene locus SGO_0174 in contig CP000725_GR.
KEGG	sgo:SGO_0174.
PATRIC	19658307. VBIStrGor124371_0175.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000027201.
HOGENOM	HBG628189.
OMA	DKETAND.
ProtClustDB	PRK01406.
Enzyme and pathway databases
BioCyc	SGOR29390:SGO_0174-MONOMER.
Family and domain databases
HAMAP	MF_00022_B. Glu_tRNA_synth_B. [Tree]
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR020751. aa-tRNA-synth_I_codon-bd_sub2. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-synth_Ib_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits. G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
KO	K09698.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit. PTHR10119:SF1. PTHR10119:SF1. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF48163. tRNA-synt_bind. 1 hit.
TIGRFAMs	TIGR00464. GltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE_STRGC

Accession

Primary (citable) accession number: A8AUN5

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	October 23, 2007
Last modified:	January 25, 2012
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program