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Protein

2,3-diketo-L-gulonate reductase

Gene

dlgD

Organism
Citrobacter koseri (strain ATCC BAA-895 / CDC 4225-83 / SGSC4696)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the reduction of 2,3-diketo-L-gulonate in the presence of NADH, to form 3-keto-L-gulonate.UniRule annotation

Catalytic activityi

3-dehydro-L-gulonate + NAD(P)+ = (4R,5S)-4,5,6-trihydroxy-2,3-dioxohexanoate + NAD(P)H.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei44Proton donorUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi168 – 174NADUniRule annotation7
Nucleotide bindingi224 – 225NADUniRule annotation2
Nucleotide bindingi304 – 306NADUniRule annotation3

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NAD

Names & Taxonomyi

Protein namesi
Recommended name:
2,3-diketo-L-gulonate reductaseUniRule annotation (EC:1.1.1.130UniRule annotation)
Short name:
2,3-DKG reductaseUniRule annotation
Alternative name(s):
3-dehydro-L-gulonate 2-dehydrogenaseUniRule annotation
Gene namesi
Name:dlgDUniRule annotation
Ordered Locus Names:CKO_05033
OrganismiCitrobacter koseri (strain ATCC BAA-895 / CDC 4225-83 / SGSC4696)
Taxonomic identifieri290338 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeCitrobacter
Proteomesi
  • UP000008148 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000624381 – 3322,3-diketo-L-gulonate reductaseAdd BLAST332

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi290338.CKO_05033.

Structurei

3D structure databases

ProteinModelPortaliA8ARG3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH2/MDH2 oxidoreductase family. DlgD subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105DG4. Bacteria.
COG2055. LUCA.
HOGENOMiHOG000249660.
KOiK08092.
OMAiAIKNTNH.
OrthoDBiPOG091H0T0F.

Family and domain databases

HAMAPiMF_00820. Diketo_gul_reduc. 1 hit.
InterProiIPR023689. Diketo_gul_Rdtase.
IPR003767. Malate/L-lactate_DH.
[Graphical view]
PANTHERiPTHR11091. PTHR11091. 1 hit.
PfamiPF02615. Ldh_2. 1 hit.
[Graphical view]
SUPFAMiSSF89733. SSF89733. 1 hit.

Sequencei

Sequence statusi: Complete.

A8ARG3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKVTFEQLKE AFNRVLLARG VAAETADACA EMFARTTESG VYSHGVNRFP
60 70 80 90 100
RFIQQLDNGD IIPDARPQRI TSLGAIEQWD AQRAIGNLTA KKMMDRAIEL
110 120 130 140 150
ASDHGIGLVA LRNANHWMRG GSYGWQAAEK GYIGICWTNS IAVMPPWGSK
160 170 180 190 200
ECRIGTNPLI VAIPSSPITM IDMSMSMFSY GMLEVNRLAG RELPVDGGFD
210 220 230 240 250
DEGNLTKEPG VIEKNRRILP MGYWKGSGLS IVLDMIATLL SDGASVAEVT
260 270 280 290 300
QENSDEYGVS QIFIAIEVDK LIDGATRDAK LQRIMDFVTT AERADENVAI
310 320 330
RLPGHEFTRL RDENRRNGIT VDDSVWAKIQ AL
Length:332
Mass (Da):36,653
Last modified:October 23, 2007 - v1
Checksum:i8407DBFF25D15CF5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000822 Genomic DNA. Translation: ABV16076.1.
RefSeqiWP_012135714.1. NC_009792.1.

Genome annotation databases

EnsemblBacteriaiABV16076; ABV16076; CKO_05033.
KEGGicko:CKO_05033.
PATRICi20392105. VBICitKos71230_4199.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000822 Genomic DNA. Translation: ABV16076.1.
RefSeqiWP_012135714.1. NC_009792.1.

3D structure databases

ProteinModelPortaliA8ARG3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi290338.CKO_05033.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABV16076; ABV16076; CKO_05033.
KEGGicko:CKO_05033.
PATRICi20392105. VBICitKos71230_4199.

Phylogenomic databases

eggNOGiENOG4105DG4. Bacteria.
COG2055. LUCA.
HOGENOMiHOG000249660.
KOiK08092.
OMAiAIKNTNH.
OrthoDBiPOG091H0T0F.

Family and domain databases

HAMAPiMF_00820. Diketo_gul_reduc. 1 hit.
InterProiIPR023689. Diketo_gul_Rdtase.
IPR003767. Malate/L-lactate_DH.
[Graphical view]
PANTHERiPTHR11091. PTHR11091. 1 hit.
PfamiPF02615. Ldh_2. 1 hit.
[Graphical view]
SUPFAMiSSF89733. SSF89733. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiDLGD_CITK8
AccessioniPrimary (citable) accession number: A8ARG3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: October 23, 2007
Last modified: November 2, 2016
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.