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A8AQ58 (IF2_CITK8) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 46. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Translation initiation factor IF-2
Gene names
Name:infB
Ordered Locus Names:CKO_04569
OrganismCitrobacter koseri (strain ATCC BAA-895 / CDC 4225-83 / SGSC4696) [Complete proteome] [HAMAP]
Taxonomic identifier290338 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeCitrobacter

Protein attributes

Sequence length894 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex By similarity. HAMAP-Rule MF_00100

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00100.

Sequence similarities

Belongs to the IF-2 family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandGTP-binding
Nucleotide-binding
   Molecular functionInitiation factor
   Technical termComplete proteome
Gene Ontology (GO)
   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionGTP binding

Inferred from electronic annotation. Source: UniProtKB-KW

GTPase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

translation initiation factor activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 894894Translation initiation factor IF-2 HAMAP-Rule MF_00100
PRO_1000008227

Regions

Nucleotide binding402 – 4098GTP By similarity
Nucleotide binding448 – 4525GTP By similarity
Nucleotide binding502 – 5054GTP By similarity
Region396 – 544149G-domain HAMAP-Rule MF_00100

Sequences

Sequence LengthMass (Da)Tools
A8AQ58 [UniParc].

Last modified October 23, 2007. Version 1.
Checksum: 9AE09ED9309727E5

FASTA89497,560
        10         20         30         40         50         60 
MTDVTVKALA AEIQTSVDRL VQQFADAGIP KSADDSVSAQ EKQTLLAHLN RENGSGPDKL 

        70         80         90        100        110        120 
TLQRKTRSTL NIPGTGGKSK SVQIEVRKKR TFVKRDPQET ERLAAEEQAQ REAEEQARRE 

       130        140        150        160        170        180 
AEETAKREAQ QKADREAAEQ AKRDAAEKAK REAAEKDKVS NQQTDDMTKT AQAEKARREN 

       190        200        210        220        230        240 
EAAELKRKAE EEARRKLEEE ARRVAEEARR MAEENKWTDN AEPTEDTSDY HVTTSQHARQ 

       250        260        270        280        290        300 
AEDENDREVE GGRGRSRNAK AARPAKKGNK HSESKADREE ARAAVRGGKG GKNRKGSALQ 

       310        320        330        340        350        360 
QSFQKPVQAV NRDVVIGETI TVGELANKMA VKGSQVIKAM MKLGAMATIN QVIDQETAQL 

       370        380        390        400        410        420 
VAEEMGHKVI LRRENELEEA VMSDRDTGAA AEPRAPVVTI MGHVDHGKTS LLDYIRSTKV 

       430        440        450        460        470        480 
ASGEAGGITQ HIGAYHVETD NGMITFLDTP GHAAFTSMRA RGAQATDIVV LVVAADDGVM 

       490        500        510        520        530        540 
PQTIEAIQHA KAAGVPVVVA VNKIDKPEAD PDRVKNELSQ YGILPEEWGG ESQFVHVSAK 

       550        560        570        580        590        600 
AGTGIDELLD AILLQAEVLE LKAVRKGMAS GAVIESFLDK GRGPVATVLV REGTLNKGDI 

       610        620        630        640        650        660 
VLCGFEYGRV RAMRNELGQE VLEAGPSIPV EILGLSGVPA AGDEVTVVRD EKKAREVALY 

       670        680        690        700        710        720 
RQGKFREVKL ARQQKSKLEN MFANMTEGEV HEVNIVLKAD VQGSVEAISD SLLKLSTDEV 

       730        740        750        760        770        780 
KVKIIGSGVG GITETDATLA AASNAILVGF NVRADASARK VIEAESLDLR YYSVIYNLID 

       790        800        810        820        830        840 
EVKAAMSGML SPELKQQIIG LAEVRDVFKS PKFGAVAGCM VTEGTIKRHN PIRVLRDNVV 

       850        860        870        880        890 
IYEGELESLR RFKDDVNEVR NGMECGIGVK NYNDVRVGDM IEVFEIIEIQ RTIA 

« Hide

References

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000822 Genomic DNA. Translation: ABV15622.1.
RefSeqYP_001456057.1. NC_009792.1.

3D structure databases

ProteinModelPortalA8AQ58.
SMRA8AQ58. Positions 2-50.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING290338.CKO_04569.

Proteomic databases

PRIDEA8AQ58.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABV15622; ABV15622; CKO_04569.
GeneID5581273.
KEGGcko:CKO_04569.
PATRIC20391321. VBICitKos71230_3816.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0532.
HOGENOMHOG000076907.
KOK02519.
OMARTGGYSQ.
OrthoDBEOG67HJSV.
ProtClustDBPRK05306.

Enzyme and pathway databases

BioCycCKOS290338:GJ8L-4554-MONOMER.

Family and domain databases

Gene3D3.40.50.10050. 1 hit.
3.40.50.300. 1 hit.
HAMAPMF_00100_B. IF_2_B.
InterProIPR009061. DNA-bd_dom_put.
IPR000795. EF_GTP-bd_dom.
IPR013575. IF2_assoc_dom_bac.
IPR006847. IF2_N.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR000178. TF_IF2_bacterial-like.
IPR015760. TIF_IF2.
IPR023115. TIF_IF2_dom3.
IPR009000. Transl_B-barrel.
IPR004161. Transl_elong_EFTu/EF1A_2.
[Graphical view]
PANTHERPTHR23115:SF41. PTHR23115:SF41. 1 hit.
PfamPF00009. GTP_EFTU. 1 hit.
PF03144. GTP_EFTU_D2. 2 hits.
PF11987. IF-2. 1 hit.
PF08364. IF2_assoc. 1 hit.
PF04760. IF2_N. 2 hits.
[Graphical view]
SUPFAMSSF46955. SSF46955. 1 hit.
SSF50447. SSF50447. 2 hits.
SSF52156. SSF52156. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsTIGR00487. IF-2. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEPS01176. IF2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameIF2_CITK8
AccessionPrimary (citable) accession number: A8AQ58
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: October 23, 2007
Last modified: April 16, 2014
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Translation initiation factors

List of translation initiation factor entries