A8APY0 (RLMG_CITK8) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosomal RNA large subunit methyltransferase G
EC=2.1.1.174

Alternative name(s):
23S rRNA m2G1835 methyltransferase
rRNA (guanine-N(2)-)-methyltransferase RlmG

Gene names

Name:	rlmG
Ordered Locus Names:	CKO_04488

Organism

Citrobacter koseri (strain ATCC BAA-895 / CDC 4225-83 / SGSC4696) [Complete proteome] [HAMAP]

Taxonomic identifier

290338 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Citrobacter

Protein attributes

Sequence length	378 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically methylates the guanosine in position 1835 (m2G1835) of 23S rRNA By similarity. HAMAP MF_01859
Catalytic activity	S-adenosyl-L-methionine + guanosine(1835) in 23S rRNA = S-adenosyl-L-homocysteine + N(2)-methylguanosine(1835) in 23S rRNA. HAMAP MF_01859
Subcellular location	Cytoplasm Potential HAMAP MF_01859.
Sequence similarities	Belongs to the methyltransferase superfamily. RlmG family.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	nucleic acid binding Inferred from electronic annotation. Source: InterPro rRNA (guanine-N2-)-methyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 378	378	Ribosomal RNA large subunit methyltransferase G HAMAP MF_01859		PRO_0000366449

Sequences

	Sequence		Length	Mass (Da)
	A8APY0 [UniParc]. Last modified October 23, 2007. Version 1. Checksum: D865496D29D15903	FASTA	378	42,098
	10 20 30 40 50 60 MSHVDNGFRS LTLKRFPETD DVNPLQAWEA ADEYLLQQLD DTEISGPLLI LNDAFGALSC 70 80 90 100 110 120 ALAEHTPYSI GDSYLSELAT RENLRHNDIA ESSVKFLDST AAYPPAPGVV LIKVPKTLAL 130 140 150 160 170 180 LEQQLRALRQ VVTPQTRIIA GAKARDIHTS TLELFEKVLG PTTTTLAWKK ARLINCTFSK 190 200 210 220 230 240 PELADAPQTL SWKLDGTDWT IHNHANVFSR TGLDIGARFF MQHLPENLEG EIVDLGCGNG 250 260 270 280 290 300 VIGLTLLAKN PQASVVFVDE SPMAVASSRL NVETNMPEAL DRCEFMINNA LSGVEPFRFN 310 320 330 340 350 360 AVLCNPPFHQ KHALTDNVAW EMFHHARRCL KINGELYIVA NRHLDYFHKL KKIFGNCTTI 370 ATNNKFVVLK AVKTGRRR

References

[1]

The Citrobacter koseri Genome Sequencing Project McClelland M., Sanderson E.K., Porwollik S., Spieth J., Clifton W.S., Latreille P., Courtney L., Wang C., Pepin K., Bhonagiri V., Nash W., Johnson M., Thiruvilangam P., Wilson R. Submitted (AUG-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-895 / CDC 4225-83 / SGSC4696.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000822 Genomic DNA. Translation: ABV15543.1.
RefSeq	YP_001455979.1. NC_009792.1.
3D structure databases
ProteinModelPortal	A8APY0.
ModBase	Search...
Protein-protein interaction databases
STRING	A8APY0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5584454.
GenomeReviews	Gene locus CKO_04488 in contig CP000822_GR.
KEGG	cko:CKO_04488.
PATRIC	20391185. VBICitKos71230_3748.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2813.
HOGENOM	HBG321931.
OMA	NRHLGYH.
ProtClustDB	PRK15001.
Family and domain databases
HAMAP	MF_01859. 23SrRNA_methyltr_G. [Tree]
InterPro	IPR002052. DNA_methylase_N6_adenine_CS. IPR017237. rRNA_m2G-MeTrfase_RsmD. IPR007848. Small_mtfrase. [Graphical view]
KO	K11391.
Pfam	PF05175. MTS. 1 hit. [Graphical view]
PIRSF	PIRSF037565. RRNA_m2G_Mtase_RsmD_prd. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RLMG_CITK8

Accession

Primary (citable) accession number: A8APY0

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	October 23, 2007
Last modified:	January 25, 2012
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program