A8AP56 (AAS_CITK8) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Bifunctional protein aas

Including the following 2 domains:

1. 2-acylglycerophosphoethanolamine acyltransferase
   EC=2.3.1.40
   Alternative name(s):
   2-acyl-GPE acyltransferase
   Acyl-[acyl-carrier-protein]--phospholipid O-acyltransferase
2. Acyl-[acyl-carrier-protein] synthetase
   EC=6.2.1.20
   Alternative name(s):
   Acyl-ACP synthetase
   Long-chain-fatty-acid--[acyl-carrier-protein] ligase

Gene names

Name:	aas
Ordered Locus Names:	CKO_04208

Organism

Citrobacter koseri (strain ATCC BAA-895 / CDC 4225-83 / SGSC4696) [Complete proteome] [HAMAP]

Taxonomic identifier

290338 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Citrobacter

Protein attributes

Sequence length	719 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Plays a role in lysophospholipid acylation. Transfers fatty acids to the 1-position via an enzyme-bound acyl-ACP intermediate in the presence of ATP and magnesium. Its physiological function is to regenerate phosphatidylethanolamine from 2-acyl-glycero-3-phosphoethanolamine (2-acyl-GPE) formed by transacylation reactions or degradation by phospholipase A1 By similarity. HAMAP MF_01162
Catalytic activity	Acyl-[acyl-carrier-protein] + O-(2-acyl-sn-glycero-3-phospho)ethanolamine = [acyl-carrier-protein] + O-(1-beta-acyl-2-acyl-sn-glycero-3-phospho)ethanolamine. HAMAP MF_01162 ATP + an acid + [acyl-carrier-protein] = AMP + diphosphate + acyl-[acyl-carrier-protein]. HAMAP MF_01162
Subcellular location	Cell inner membrane; Multi-pass membrane protein By similarity HAMAP MF_01162.
Sequence similarities	In the N-terminal section; belongs to the 2-acyl-GPE acetyltransferase family. In the C-terminal section; belongs to the ATP-dependent AMP-binding enzyme family.

Ontologies

Keywords
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Ligand	ATP-binding Nucleotide-binding
Molecular function	Acyltransferase Ligase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	fatty acid metabolic process Inferred from electronic annotation. Source: InterPro phospholipid biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW acyl-[acyl-carrier-protein]-phospholipid O-acyltransferase activity Inferred from electronic annotation. Source: EC long-chain fatty acid [acyl-carrier-protein] ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 719	719	Bifunctional protein aas HAMAP MF_01162		PRO_1000065633
Regions
	Transmembrane	258 – 277	20	Helical; Potential
	Transmembrane	409 – 433	25	Helical; Potential
	Region	15 – 138	124	Acyltransferase HAMAP MF_01162
	Region	233 – 646	414	AMP-binding HAMAP MF_01162
Sites
	Active site	36	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A8AP56 [UniParc]. Last modified October 23, 2007. Version 1. Checksum: F07DF27821C006F3	FASTA	719	80,223
	10 20 30 40 50 60 MLFGFFRNLF RVLYRVRVTG DARALQGERI LITPNHVSFI DGILLALFLP VRPVFAVYSS 70 80 90 100 110 120 ISQQWYMRWL KSLIDFVPLD PTKPMAIKHL VRLVEQGRPV VIFPEGRISV SGSLMKIYDG 130 140 150 160 170 180 AGFVAAKSGA TVVPVRIEGA ELTHFSRLKG LVKQRFFPRI HLHILPPTHL PMPEAPRARE 190 200 210 220 230 240 RRKLAGEMLH QIMMEARMAV RPRETLYESL LAAQYRYGAG KNCVEDINFT PDTYRKLLTK 250 260 270 280 290 300 TLFVGRILEK YSAQGENIGL MLPNAAISAA VIFGAVSRGR IPAMMNYTAG VKGLTSAITA 310 320 330 340 350 360 AEIKTVFTSR QFLEKGKLWH LPEQLTQVRW IYLEDLKADV TLADKLWIFA HLLMPRLAQV 370 380 390 400 410 420 KQRPEEAAMI LFTSGSEGHP KGVVHSHKSL LANVEQIKTI ADFTANDRFM SALPLFHSFG 430 440 450 460 470 480 LTVGLLTPLL TGAEVFLYPS PLHYRIVPEL VYDRNCTVLF GTSTFLGNYA RFANPYDFYR 490 500 510 520 530 540 LRYVVAGAEK LQDSTRQLWQ DKFGLRVLEG YGVTECAPVV SINVPMAAKP GTVGRILPGM 550 560 570 580 590 600 DARLLAVPGI DDGGRLQLKG PNIMNGYLRV EKPGVLEAPA AENALGDVEQ GWYDTGDIVR 610 620 630 640 650 660 FDENGFVQIQ GRAKRFAKIA GEMVSLEMVE QLALGVSPDK MHATAIKSDA SKGEALVLFT 670 680 690 700 710 TDGELTRESL LQYARTHGIP ELAVPRDIRY LKQLPLLGSG KPDFVTLKSW VDEPEKQHA

References

[1]

The Citrobacter koseri Genome Sequencing Project McClelland M., Sanderson E.K., Porwollik S., Spieth J., Clifton W.S., Latreille P., Courtney L., Wang C., Pepin K., Bhonagiri V., Nash W., Johnson M., Thiruvilangam P., Wilson R. Submitted (AUG-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-895 / CDC 4225-83 / SGSC4696.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000822 Genomic DNA. Translation: ABV15269.1.
RefSeq	YP_001455705.1. NC_009792.1.
3D structure databases
ProteinModelPortal	A8AP56.
ModBase	Search...
Protein-protein interaction databases
STRING	A8AP56.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5582725.
GenomeReviews	Gene locus CKO_04208 in contig CP000822_GR.
KEGG	cko:CKO_04208.
PATRIC	20390715. VBICitKos71230_3516.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0318.
HOGENOM	HBG361068.
OMA	ANWVYLE.
ProtClustDB	PRK08043.
Family and domain databases
HAMAP	MF_01162. Aas. [Tree]
InterPro	IPR002123. Acyltransferase. IPR020845. AMP-binding_CS. IPR000873. AMP-dep_Synth/Lig. IPR023775. Bifunctional_Aas. [Graphical view]
KO	K05939.
Pfam	PF01553. Acyltransferase. 1 hit. PF00501. AMP-binding. 1 hit. [Graphical view]
SMART	SM00563. PlsC. 1 hit. [Graphical view]
PROSITE	PS00455. AMP_BINDING. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AAS_CITK8

Accession

Primary (citable) accession number: A8AP56

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	October 23, 2007
Last modified:	January 25, 2012
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program