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A8ANX0

- CYSI_CITK8

UniProt

A8ANX0 - CYSI_CITK8

Protein

Sulfite reductase [NADPH] hemoprotein beta-component

Gene

cysI

Organism
Citrobacter koseri (strain ATCC BAA-895 / CDC 4225-83 / SGSC4696)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 47 (01 Oct 2014)
      Sequence version 1 (23 Oct 2007)
      Previous versions | rss
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    Functioni

    Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L-cysteine from sulfate.UniRule annotation

    Catalytic activityi

    H2S + 3 NADP+ + 3 H2O = sulfite + 3 NADPH.UniRule annotation

    Cofactori

    Binds 1 siroheme per subunit.UniRule annotation
    Binds 1 4Fe-4S cluster per subunit.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi434 – 4341Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi440 – 4401Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi479 – 4791Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi483 – 4831Iron (siroheme axial ligand)UniRule annotation
    Metal bindingi483 – 4831Iron-sulfur (4Fe-4S)UniRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    2. heme binding Source: InterPro
    3. metal ion binding Source: UniProtKB-KW
    4. NADP binding Source: InterPro
    5. sulfite reductase (NADPH) activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. cysteine biosynthetic process Source: UniProtKB-KW
    2. hydrogen sulfide biosynthetic process Source: UniProtKB-HAMAP
    3. sulfate assimilation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Amino-acid biosynthesis, Cysteine biosynthesis

    Keywords - Ligandi

    4Fe-4S, Heme, Iron, Iron-sulfur, Metal-binding, NADP

    Enzyme and pathway databases

    BioCyciCKOS290338:GJ8L-4107-MONOMER.
    UniPathwayiUPA00140; UER00207.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Sulfite reductase [NADPH] hemoprotein beta-componentUniRule annotation (EC:1.8.1.2UniRule annotation)
    Short name:
    SiR-HPUniRule annotation
    Short name:
    SiRHPUniRule annotation
    Gene namesi
    Name:cysIUniRule annotation
    Ordered Locus Names:CKO_04117
    OrganismiCitrobacter koseri (strain ATCC BAA-895 / CDC 4225-83 / SGSC4696)
    Taxonomic identifieri290338 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeCitrobacter
    ProteomesiUP000008148: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. sulfite reductase complex (NADPH) Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 570570Sulfite reductase [NADPH] hemoprotein beta-componentPRO_1000068757Add
    BLAST

    Proteomic databases

    PRIDEiA8ANX0.

    Interactioni

    Subunit structurei

    Alpha(8)-beta8. The alpha component is a flavoprotein, the beta component is a hemoprotein.UniRule annotation

    Protein-protein interaction databases

    STRINGi290338.CKO_04117.

    Structurei

    3D structure databases

    ProteinModelPortaliA8ANX0.
    SMRiA8ANX0. Positions 81-570.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the nitrite and sulfite reductase 4Fe-4S domain family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0155.
    HOGENOMiHOG000218418.
    KOiK00381.
    OMAiELHQEAY.
    OrthoDBiEOG6W9XFB.

    Family and domain databases

    Gene3Di3.90.480.10. 2 hits.
    HAMAPiMF_01540. CysI.
    InterProiIPR011786. CysI.
    IPR005117. NiRdtase/SiRdtase_haem-b_fer.
    IPR006067. NO2/SO3_Rdtase_4Fe4S_dom.
    IPR006066. NO2/SO3_Rdtase_FeS/sirohaem_BS.
    [Graphical view]
    PfamiPF01077. NIR_SIR. 1 hit.
    PF03460. NIR_SIR_ferr. 2 hits.
    [Graphical view]
    PRINTSiPR00397. SIROHAEM.
    SUPFAMiSSF55124. SSF55124. 2 hits.
    TIGRFAMsiTIGR02041. CysI. 1 hit.
    PROSITEiPS00365. NIR_SIR. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A8ANX0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSEKHPGPLV VEGKLADAER MKLESNYLRG TIAEDLNDGL TGGFKGDNFL    50
    LIRFHGMYQQ DDRDIRAERA EQKLEPRHAM LLRCRLPGGV ITTRQWQAID 100
    KFAAENTIYG SIRLTNRQTF QFHGILKKNV KPVHQMLHSV GLDALATAND 150
    MNRNVLCTSN PYESELHAEA YEWAKKISEH LLPRTRAYAE IWLDQEKVAT 200
    TDEEPILGQT YLPRKFKTTV VIPPQNDIDL HANDMNFVAV AENGKLVGFN 250
    LLVGGGLSIE HGNKKTYART ASEFGYLPLE HTLAVAEAVV TTQRDWGNRT 300
    DRKNAKTKYT LERVGVETFK AEVERRAGIK FEPIRPYEFT GRGDRIGWVK 350
    GIDNKWHLTL FIENGRILDY PGRPLKTGLL EIAKIHKGEF RITANQNLII 400
    AGVPESQKAK IEKLARNHGL MNAVTSQREN SMACVSFPTC PLAMAEAERF 450
    LPTFVDKVDA LMTHHGVSDE HIVLRVTGCP NGCGRAMLAE VGLVGKAPGR 500
    YNLHLGGNRI GTRIPRMFKE NITEPEILAS LDELIGRWVK EREAGEGFGD 550
    FTVRAGIIRP VLDPARDFWE 570
    Length:570
    Mass (Da):64,076
    Last modified:October 23, 2007 - v1
    Checksum:i63346FE6C3BEDDE8
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000822 Genomic DNA. Translation: ABV15183.1.
    RefSeqiWP_012134872.1. NC_009792.1.
    YP_001455619.1. NC_009792.1.

    Genome annotation databases

    EnsemblBacteriaiABV15183; ABV15183; CKO_04117.
    GeneIDi5584355.
    KEGGicko:CKO_04117.
    PATRICi20390573. VBICitKos71230_3448.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000822 Genomic DNA. Translation: ABV15183.1 .
    RefSeqi WP_012134872.1. NC_009792.1.
    YP_001455619.1. NC_009792.1.

    3D structure databases

    ProteinModelPortali A8ANX0.
    SMRi A8ANX0. Positions 81-570.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 290338.CKO_04117.

    Proteomic databases

    PRIDEi A8ANX0.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABV15183 ; ABV15183 ; CKO_04117 .
    GeneIDi 5584355.
    KEGGi cko:CKO_04117.
    PATRICi 20390573. VBICitKos71230_3448.

    Phylogenomic databases

    eggNOGi COG0155.
    HOGENOMi HOG000218418.
    KOi K00381.
    OMAi ELHQEAY.
    OrthoDBi EOG6W9XFB.

    Enzyme and pathway databases

    UniPathwayi UPA00140 ; UER00207 .
    BioCyci CKOS290338:GJ8L-4107-MONOMER.

    Family and domain databases

    Gene3Di 3.90.480.10. 2 hits.
    HAMAPi MF_01540. CysI.
    InterProi IPR011786. CysI.
    IPR005117. NiRdtase/SiRdtase_haem-b_fer.
    IPR006067. NO2/SO3_Rdtase_4Fe4S_dom.
    IPR006066. NO2/SO3_Rdtase_FeS/sirohaem_BS.
    [Graphical view ]
    Pfami PF01077. NIR_SIR. 1 hit.
    PF03460. NIR_SIR_ferr. 2 hits.
    [Graphical view ]
    PRINTSi PR00397. SIROHAEM.
    SUPFAMi SSF55124. SSF55124. 2 hits.
    TIGRFAMsi TIGR02041. CysI. 1 hit.
    PROSITEi PS00365. NIR_SIR. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC BAA-895 / CDC 4225-83 / SGSC4696.

    Entry informationi

    Entry nameiCYSI_CITK8
    AccessioniPrimary (citable) accession number: A8ANX0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 5, 2008
    Last sequence update: October 23, 2007
    Last modified: October 1, 2014
    This is version 47 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3