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A8ANX0

- CYSI_CITK8

UniProt

A8ANX0 - CYSI_CITK8

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Protein
Sulfite reductase [NADPH] hemoprotein beta-component
Gene
cysI, CKO_04117
Organism
Citrobacter koseri (strain ATCC BAA-895 / CDC 4225-83 / SGSC4696)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L-cysteine from sulfate By similarity.UniRule annotation

Catalytic activityi

H2S + 3 NADP+ + 3 H2O = sulfite + 3 NADPH.UniRule annotation

Cofactori

Binds 1 siroheme per subunit By similarity.UniRule annotation
Binds 1 4Fe-4S cluster per subunit By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi434 – 4341Iron-sulfur (4Fe-4S) By similarity
Metal bindingi440 – 4401Iron-sulfur (4Fe-4S) By similarity
Metal bindingi479 – 4791Iron-sulfur (4Fe-4S) By similarity
Metal bindingi483 – 4831Iron (siroheme axial ligand) By similarity
Metal bindingi483 – 4831Iron-sulfur (4Fe-4S) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  2. NADP binding Source: InterPro
  3. heme binding Source: InterPro
  4. metal ion binding Source: UniProtKB-KW
  5. sulfite reductase (NADPH) activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. cysteine biosynthetic process Source: UniProtKB-KW
  2. hydrogen sulfide biosynthetic process Source: UniProtKB-HAMAP
  3. sulfate assimilation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Amino-acid biosynthesis, Cysteine biosynthesis

Keywords - Ligandi

4Fe-4S, Heme, Iron, Iron-sulfur, Metal-binding, NADP

Enzyme and pathway databases

BioCyciCKOS290338:GJ8L-4107-MONOMER.
UniPathwayiUPA00140; UER00207.

Names & Taxonomyi

Protein namesi
Recommended name:
Sulfite reductase [NADPH] hemoprotein beta-component (EC:1.8.1.2)
Short name:
SiR-HP
Short name:
SiRHP
Gene namesi
Name:cysI
Ordered Locus Names:CKO_04117
OrganismiCitrobacter koseri (strain ATCC BAA-895 / CDC 4225-83 / SGSC4696)
Taxonomic identifieri290338 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeCitrobacter
ProteomesiUP000008148: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. sulfite reductase complex (NADPH) Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 570570Sulfite reductase [NADPH] hemoprotein beta-componentUniRule annotation
PRO_1000068757Add
BLAST

Proteomic databases

PRIDEiA8ANX0.

Interactioni

Subunit structurei

Alpha(8)-beta8. The alpha component is a flavoprotein, the beta component is a hemoprotein By similarity.

Protein-protein interaction databases

STRINGi290338.CKO_04117.

Structurei

3D structure databases

ProteinModelPortaliA8ANX0.
SMRiA8ANX0. Positions 81-570.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0155.
HOGENOMiHOG000218418.
KOiK00381.
OMAiELHQEAY.
OrthoDBiEOG6W9XFB.

Family and domain databases

Gene3Di3.90.480.10. 2 hits.
HAMAPiMF_01540. CysI.
InterProiIPR011786. CysI.
IPR005117. NiRdtase/SiRdtase_haem-b_fer.
IPR006067. NO2/SO3_Rdtase_4Fe4S_dom.
IPR006066. NO2/SO3_Rdtase_FeS/sirohaem_BS.
[Graphical view]
PfamiPF01077. NIR_SIR. 1 hit.
PF03460. NIR_SIR_ferr. 2 hits.
[Graphical view]
PRINTSiPR00397. SIROHAEM.
SUPFAMiSSF55124. SSF55124. 2 hits.
TIGRFAMsiTIGR02041. CysI. 1 hit.
PROSITEiPS00365. NIR_SIR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A8ANX0-1 [UniParc]FASTAAdd to Basket

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MSEKHPGPLV VEGKLADAER MKLESNYLRG TIAEDLNDGL TGGFKGDNFL    50
LIRFHGMYQQ DDRDIRAERA EQKLEPRHAM LLRCRLPGGV ITTRQWQAID 100
KFAAENTIYG SIRLTNRQTF QFHGILKKNV KPVHQMLHSV GLDALATAND 150
MNRNVLCTSN PYESELHAEA YEWAKKISEH LLPRTRAYAE IWLDQEKVAT 200
TDEEPILGQT YLPRKFKTTV VIPPQNDIDL HANDMNFVAV AENGKLVGFN 250
LLVGGGLSIE HGNKKTYART ASEFGYLPLE HTLAVAEAVV TTQRDWGNRT 300
DRKNAKTKYT LERVGVETFK AEVERRAGIK FEPIRPYEFT GRGDRIGWVK 350
GIDNKWHLTL FIENGRILDY PGRPLKTGLL EIAKIHKGEF RITANQNLII 400
AGVPESQKAK IEKLARNHGL MNAVTSQREN SMACVSFPTC PLAMAEAERF 450
LPTFVDKVDA LMTHHGVSDE HIVLRVTGCP NGCGRAMLAE VGLVGKAPGR 500
YNLHLGGNRI GTRIPRMFKE NITEPEILAS LDELIGRWVK EREAGEGFGD 550
FTVRAGIIRP VLDPARDFWE 570
Length:570
Mass (Da):64,076
Last modified:October 23, 2007 - v1
Checksum:i63346FE6C3BEDDE8
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000822 Genomic DNA. Translation: ABV15183.1.
RefSeqiWP_012134872.1. NC_009792.1.
YP_001455619.1. NC_009792.1.

Genome annotation databases

EnsemblBacteriaiABV15183; ABV15183; CKO_04117.
GeneIDi5584355.
KEGGicko:CKO_04117.
PATRICi20390573. VBICitKos71230_3448.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000822 Genomic DNA. Translation: ABV15183.1 .
RefSeqi WP_012134872.1. NC_009792.1.
YP_001455619.1. NC_009792.1.

3D structure databases

ProteinModelPortali A8ANX0.
SMRi A8ANX0. Positions 81-570.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 290338.CKO_04117.

Proteomic databases

PRIDEi A8ANX0.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABV15183 ; ABV15183 ; CKO_04117 .
GeneIDi 5584355.
KEGGi cko:CKO_04117.
PATRICi 20390573. VBICitKos71230_3448.

Phylogenomic databases

eggNOGi COG0155.
HOGENOMi HOG000218418.
KOi K00381.
OMAi ELHQEAY.
OrthoDBi EOG6W9XFB.

Enzyme and pathway databases

UniPathwayi UPA00140 ; UER00207 .
BioCyci CKOS290338:GJ8L-4107-MONOMER.

Family and domain databases

Gene3Di 3.90.480.10. 2 hits.
HAMAPi MF_01540. CysI.
InterProi IPR011786. CysI.
IPR005117. NiRdtase/SiRdtase_haem-b_fer.
IPR006067. NO2/SO3_Rdtase_4Fe4S_dom.
IPR006066. NO2/SO3_Rdtase_FeS/sirohaem_BS.
[Graphical view ]
Pfami PF01077. NIR_SIR. 1 hit.
PF03460. NIR_SIR_ferr. 2 hits.
[Graphical view ]
PRINTSi PR00397. SIROHAEM.
SUPFAMi SSF55124. SSF55124. 2 hits.
TIGRFAMsi TIGR02041. CysI. 1 hit.
PROSITEi PS00365. NIR_SIR. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-895 / CDC 4225-83 / SGSC4696.

Entry informationi

Entry nameiCYSI_CITK8
AccessioniPrimary (citable) accession number: A8ANX0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: October 23, 2007
Last modified: September 3, 2014
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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