Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

A8ANX0 (CYSI_CITK8) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 45. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Sulfite reductase [NADPH] hemoprotein beta-component

Short name=SiR-HP
Short name=SiRHP
EC=1.8.1.2
Gene names
Name:cysI
Ordered Locus Names:CKO_04117
OrganismCitrobacter koseri (strain ATCC BAA-895 / CDC 4225-83 / SGSC4696) [Complete proteome] [HAMAP]
Taxonomic identifier290338 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeCitrobacter

Protein attributes

Sequence length570 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L-cysteine from sulfate By similarity. HAMAP-Rule MF_01540

Catalytic activity

H2S + 3 NADP+ + 3 H2O = sulfite + 3 NADPH. HAMAP-Rule MF_01540

Cofactor

Binds 1 siroheme per subunit By similarity. HAMAP-Rule MF_01540

Binds 1 4Fe-4S cluster per subunit By similarity. HAMAP-Rule MF_01540

Pathway

Sulfur metabolism; hydrogen sulfide biosynthesis; hydrogen sulfide from sulfite (NADPH route): step 1/1. HAMAP-Rule MF_01540

Subunit structure

Alpha(8)-beta8. The alpha component is a flavoprotein, the beta component is a hemoprotein By similarity.

Sequence similarities

Belongs to the nitrite and sulfite reductase 4Fe-4S domain family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 570570Sulfite reductase [NADPH] hemoprotein beta-component HAMAP-Rule MF_01540
PRO_1000068757

Sites

Metal binding4341Iron-sulfur (4Fe-4S) By similarity
Metal binding4401Iron-sulfur (4Fe-4S) By similarity
Metal binding4791Iron-sulfur (4Fe-4S) By similarity
Metal binding4831Iron (siroheme axial ligand) By similarity
Metal binding4831Iron-sulfur (4Fe-4S) By similarity

Sequences

Sequence LengthMass (Da)Tools
A8ANX0 [UniParc].

Last modified October 23, 2007. Version 1.
Checksum: 63346FE6C3BEDDE8

FASTA57064,076
        10         20         30         40         50         60 
MSEKHPGPLV VEGKLADAER MKLESNYLRG TIAEDLNDGL TGGFKGDNFL LIRFHGMYQQ 

        70         80         90        100        110        120 
DDRDIRAERA EQKLEPRHAM LLRCRLPGGV ITTRQWQAID KFAAENTIYG SIRLTNRQTF 

       130        140        150        160        170        180 
QFHGILKKNV KPVHQMLHSV GLDALATAND MNRNVLCTSN PYESELHAEA YEWAKKISEH 

       190        200        210        220        230        240 
LLPRTRAYAE IWLDQEKVAT TDEEPILGQT YLPRKFKTTV VIPPQNDIDL HANDMNFVAV 

       250        260        270        280        290        300 
AENGKLVGFN LLVGGGLSIE HGNKKTYART ASEFGYLPLE HTLAVAEAVV TTQRDWGNRT 

       310        320        330        340        350        360 
DRKNAKTKYT LERVGVETFK AEVERRAGIK FEPIRPYEFT GRGDRIGWVK GIDNKWHLTL 

       370        380        390        400        410        420 
FIENGRILDY PGRPLKTGLL EIAKIHKGEF RITANQNLII AGVPESQKAK IEKLARNHGL 

       430        440        450        460        470        480 
MNAVTSQREN SMACVSFPTC PLAMAEAERF LPTFVDKVDA LMTHHGVSDE HIVLRVTGCP 

       490        500        510        520        530        540 
NGCGRAMLAE VGLVGKAPGR YNLHLGGNRI GTRIPRMFKE NITEPEILAS LDELIGRWVK 

       550        560        570 
EREAGEGFGD FTVRAGIIRP VLDPARDFWE 

« Hide

References

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000822 Genomic DNA. Translation: ABV15183.1.
RefSeqYP_001455619.1. NC_009792.1.

3D structure databases

ProteinModelPortalA8ANX0.
SMRA8ANX0. Positions 81-570.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING290338.CKO_04117.

Proteomic databases

PRIDEA8ANX0.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABV15183; ABV15183; CKO_04117.
GeneID5584355.
KEGGcko:CKO_04117.
PATRIC20390573. VBICitKos71230_3448.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0155.
HOGENOMHOG000218418.
KOK00381.
OMAELHQEAY.
OrthoDBEOG6W9XFB.

Enzyme and pathway databases

BioCycCKOS290338:GJ8L-4107-MONOMER.
UniPathwayUPA00140; UER00207.

Family and domain databases

Gene3D3.90.480.10. 2 hits.
HAMAPMF_01540. CysI.
InterProIPR011786. CysI.
IPR005117. NiRdtase/SiRdtase_haem-b_fer.
IPR006067. NO2/SO3_Rdtase_4Fe4S_dom.
IPR006066. NO2/SO3_Rdtase_FeS/sirohaem_BS.
[Graphical view]
PfamPF01077. NIR_SIR. 1 hit.
PF03460. NIR_SIR_ferr. 2 hits.
[Graphical view]
PRINTSPR00397. SIROHAEM.
SUPFAMSSF55124. SSF55124. 2 hits.
TIGRFAMsTIGR02041. CysI. 1 hit.
PROSITEPS00365. NIR_SIR. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCYSI_CITK8
AccessionPrimary (citable) accession number: A8ANX0
Entry history
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: October 23, 2007
Last modified: May 14, 2014
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways