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Protein

Sulfite reductase [NADPH] hemoprotein beta-component

Gene

cysI

Organism
Citrobacter koseri (strain ATCC BAA-895 / CDC 4225-83 / SGSC4696)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L-cysteine from sulfate.UniRule annotation

Catalytic activityi

H2S + 3 NADP+ + 3 H2O = sulfite + 3 NADPH.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • sirohemeUniRule annotationNote: Binds 1 siroheme per subunit.UniRule annotation
  • [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster per subunit.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi434 – 4341Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi440 – 4401Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi479 – 4791Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi483 – 4831Iron (siroheme axial ligand)UniRule annotation
Metal bindingi483 – 4831Iron-sulfur (4Fe-4S)UniRule annotation

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  2. heme binding Source: InterPro
  3. metal ion binding Source: UniProtKB-KW
  4. NADP binding Source: InterPro
  5. sulfite reductase (NADPH) activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. cysteine biosynthetic process Source: UniProtKB-KW
  2. hydrogen sulfide biosynthetic process Source: UniProtKB-HAMAP
  3. sulfate assimilation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Amino-acid biosynthesis, Cysteine biosynthesis

Keywords - Ligandi

4Fe-4S, Heme, Iron, Iron-sulfur, Metal-binding, NADP

Enzyme and pathway databases

BioCyciCKOS290338:GJ8L-4107-MONOMER.
UniPathwayiUPA00140; UER00207.

Names & Taxonomyi

Protein namesi
Recommended name:
Sulfite reductase [NADPH] hemoprotein beta-componentUniRule annotation (EC:1.8.1.2UniRule annotation)
Short name:
SiR-HPUniRule annotation
Short name:
SiRHPUniRule annotation
Gene namesi
Name:cysIUniRule annotation
Ordered Locus Names:CKO_04117
OrganismiCitrobacter koseri (strain ATCC BAA-895 / CDC 4225-83 / SGSC4696)
Taxonomic identifieri290338 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeCitrobacter
ProteomesiUP000008148: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. sulfite reductase complex (NADPH) Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 570570Sulfite reductase [NADPH] hemoprotein beta-componentPRO_1000068757Add
BLAST

Proteomic databases

PRIDEiA8ANX0.

Interactioni

Subunit structurei

Alpha(8)-beta8. The alpha component is a flavoprotein, the beta component is a hemoprotein.UniRule annotation

Protein-protein interaction databases

STRINGi290338.CKO_04117.

Structurei

3D structure databases

ProteinModelPortaliA8ANX0.
SMRiA8ANX0. Positions 81-570.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the nitrite and sulfite reductase 4Fe-4S domain family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0155.
HOGENOMiHOG000218418.
KOiK00381.
OMAiRNSMACV.
OrthoDBiEOG6W9XFB.

Family and domain databases

Gene3Di3.90.480.10. 2 hits.
HAMAPiMF_01540. CysI.
InterProiIPR011786. CysI.
IPR005117. NiRdtase/SiRdtase_haem-b_fer.
IPR006067. NO2/SO3_Rdtase_4Fe4S_dom.
IPR006066. NO2/SO3_Rdtase_FeS/sirohaem_BS.
[Graphical view]
PfamiPF01077. NIR_SIR. 1 hit.
PF03460. NIR_SIR_ferr. 2 hits.
[Graphical view]
PRINTSiPR00397. SIROHAEM.
SUPFAMiSSF55124. SSF55124. 2 hits.
TIGRFAMsiTIGR02041. CysI. 1 hit.
PROSITEiPS00365. NIR_SIR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A8ANX0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEKHPGPLV VEGKLADAER MKLESNYLRG TIAEDLNDGL TGGFKGDNFL
60 70 80 90 100
LIRFHGMYQQ DDRDIRAERA EQKLEPRHAM LLRCRLPGGV ITTRQWQAID
110 120 130 140 150
KFAAENTIYG SIRLTNRQTF QFHGILKKNV KPVHQMLHSV GLDALATAND
160 170 180 190 200
MNRNVLCTSN PYESELHAEA YEWAKKISEH LLPRTRAYAE IWLDQEKVAT
210 220 230 240 250
TDEEPILGQT YLPRKFKTTV VIPPQNDIDL HANDMNFVAV AENGKLVGFN
260 270 280 290 300
LLVGGGLSIE HGNKKTYART ASEFGYLPLE HTLAVAEAVV TTQRDWGNRT
310 320 330 340 350
DRKNAKTKYT LERVGVETFK AEVERRAGIK FEPIRPYEFT GRGDRIGWVK
360 370 380 390 400
GIDNKWHLTL FIENGRILDY PGRPLKTGLL EIAKIHKGEF RITANQNLII
410 420 430 440 450
AGVPESQKAK IEKLARNHGL MNAVTSQREN SMACVSFPTC PLAMAEAERF
460 470 480 490 500
LPTFVDKVDA LMTHHGVSDE HIVLRVTGCP NGCGRAMLAE VGLVGKAPGR
510 520 530 540 550
YNLHLGGNRI GTRIPRMFKE NITEPEILAS LDELIGRWVK EREAGEGFGD
560 570
FTVRAGIIRP VLDPARDFWE
Length:570
Mass (Da):64,076
Last modified:October 23, 2007 - v1
Checksum:i63346FE6C3BEDDE8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000822 Genomic DNA. Translation: ABV15183.1.
RefSeqiWP_012134872.1. NC_009792.1.
YP_001455619.1. NC_009792.1.

Genome annotation databases

EnsemblBacteriaiABV15183; ABV15183; CKO_04117.
GeneIDi5584355.
KEGGicko:CKO_04117.
PATRICi20390573. VBICitKos71230_3448.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000822 Genomic DNA. Translation: ABV15183.1.
RefSeqiWP_012134872.1. NC_009792.1.
YP_001455619.1. NC_009792.1.

3D structure databases

ProteinModelPortaliA8ANX0.
SMRiA8ANX0. Positions 81-570.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi290338.CKO_04117.

Proteomic databases

PRIDEiA8ANX0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABV15183; ABV15183; CKO_04117.
GeneIDi5584355.
KEGGicko:CKO_04117.
PATRICi20390573. VBICitKos71230_3448.

Phylogenomic databases

eggNOGiCOG0155.
HOGENOMiHOG000218418.
KOiK00381.
OMAiRNSMACV.
OrthoDBiEOG6W9XFB.

Enzyme and pathway databases

UniPathwayiUPA00140; UER00207.
BioCyciCKOS290338:GJ8L-4107-MONOMER.

Family and domain databases

Gene3Di3.90.480.10. 2 hits.
HAMAPiMF_01540. CysI.
InterProiIPR011786. CysI.
IPR005117. NiRdtase/SiRdtase_haem-b_fer.
IPR006067. NO2/SO3_Rdtase_4Fe4S_dom.
IPR006066. NO2/SO3_Rdtase_FeS/sirohaem_BS.
[Graphical view]
PfamiPF01077. NIR_SIR. 1 hit.
PF03460. NIR_SIR_ferr. 2 hits.
[Graphical view]
PRINTSiPR00397. SIROHAEM.
SUPFAMiSSF55124. SSF55124. 2 hits.
TIGRFAMsiTIGR02041. CysI. 1 hit.
PROSITEiPS00365. NIR_SIR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-895 / CDC 4225-83 / SGSC4696.

Entry informationi

Entry nameiCYSI_CITK8
AccessioniPrimary (citable) accession number: A8ANX0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: October 23, 2007
Last modified: March 4, 2015
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.