Reviewed, UniProtKB/Swiss-Prot A8ANV8 (SURE_CITK8)

Names and origin

Protein names

Recommended name:
    Multifunctional protein surE
Including the following 2 domains:
    1- Recommended name:
            5'/3'-nucleotidase
              EC=3.1.3.5
              EC=3.1.3.6
        Alternative name(s):
            Nucleoside monophosphate phosphohydrolase
    2- Recommended name:
            Exopolyphosphatase
              EC=3.6.1.11

Gene names

Name:	surE
Ordered Locus Names:	CKO_04105

Organism

Citrobacter koseri (strain ATCC BAA-895 / CDC 4225-83 / SGSC4696) [Complete proteome] [HAMAP]

Taxonomic identifier

290338 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Citrobacter

Protein attributes

Sequence length	253 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Nucleotidase with a broad substrate specificity as it can dephosphorylate various ribo- and deoxyribonucleoside 5'-monophosphates and ribonucleoside 3'-monophosphates with highest affinity to 3'-AMP. Also hydrolyzes polyphosphate (exopolyphosphatase activity) with the preference for short-chain-length substrates (P20-25). Might be involved in the regulation of dNTP and NTP pools, and in the turnover of 3'-mononucleotides produced by numerous intracellular RNases (T1, T2, and F) during the degradation of various RNAs By similarity.
Catalytic activity	A 5'-ribonucleotide + H2O = a ribonucleoside + phosphate. HAMAP MF_00060 A 3'-ribonucleotide + H2O = a ribonucleoside + phosphate. HAMAP MF_00060 (Polyphosphate)(n) + H2O = (polyphosphate)(n-1) + phosphate. HAMAP MF_00060
Cofactor	Binds 1 divalent metal cation per subunit By similarity.
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the surE nucleotidase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Metal-binding Nucleotide-binding
Molecular function	Hydrolase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3'-nucleotidase activity Inferred from electronic annotation. Source: EC 5'-nucleotidase activity Inferred from electronic annotation. Source: HAMAP exopolyphosphatase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: HAMAP nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 253	253	Multifunctional protein surE HAMAP MF_00060		PRO_1000007720
Sites
	Metal binding	8	1	Divalent metal cation By similarity
	Metal binding	9	1	Divalent metal cation By similarity
	Metal binding	39	1	Divalent metal cation By similarity
	Metal binding	92	1	Divalent metal cation By similarity

Sequences

	Sequence		Length	Mass (Da)
	A8ANV8-1 [UniParc]. Last modified October 23, 2007. Version 1. Checksum: 3AF387C4A813E7FB	FASTA	253	27,075
	10 20 30 40 50 60 MRILLSNDDG VHAPGIQTLA KALREFADVQ VVAPDRNRSG ASNSLTLESS LRTFTFDNGD 70 80 90 100 110 120 IAVQMGTPTD CVYLGVNALM RPRPDIVVSG INAGPNLGDD VIYSGTVAAA MEGRHLGFPA 130 140 150 160 170 180 LAVSLDGHQH YETAAAVTCS ILRALRREPL RTGRILNINV PDLPLDQIKG IRVTRCGSRH 190 200 210 220 230 240 PADQVIPQQD PRGNTLYWIG PPGGKCDAGP DTDFAAVDEG YVSVTPLHVD LTAYSAHDVV 250 SDWLDSVGVD AQW

References

[1]	McClelland M., Sanderson E.K., Porwollik S., Spieth J., Clifton W.S., Latreille P., Courtney L., Wang C., Pepin K., Bhonagiri V., Nash W., Johnson M., Thiruvilangam P., Wilson R. Submitted (AUG-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000822 Genomic DNA. Translation: ABV15171.1.
RefSeq	YP_001455607.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5582880.
GenomeReviews	Gene locus CKO_04105 in contig CP000822_GR.
KEGG	cko:CKO_04105.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	A8ANV8. NLNIPPC.
Family and domain databases
HAMAP	MF_00060. [Tree]
InterPro	IPR002828. SurE-like_Pase/nucleotidase. [Graphical view]
Gene3D	G3DSA:3.40.1210.10. SurE-like_Pase/nucleotidase. 1 hit.
Pfam	PF01975. SurE. 1 hit. [Graphical view]
ProDom	PD005378. SurE. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00087. surE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

SURE_CITK8

Accession

Primary (citable) accession number: A8ANV8

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 23, 2007
Last modified:	June 16, 2009
This is version 12 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)