Reviewed, UniProtKB/Swiss-Prot A8AMR4 (DSBD_CITK8)

Names and origin

Protein names

Recommended name:
    Thiol:disulfide interchange protein dsbD
    EC=1.8.1.8
Alternative name(s):
    Protein-disulfide reductase
      Short name=Disulfide reductase

Gene names

Name:	dsbD
Ordered Locus Names:	CKO_03700

Organism

Citrobacter koseri (strain ATCC BAA-895 / CDC 4225-83 / SGSC4696) [Complete proteome] [HAMAP]

Taxonomic identifier

290338 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Citrobacter

Protein attributes

Sequence length	569 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Required to facilitate the formation of correct disulfide bonds in some periplasmic proteins and for the assembly of the periplasmic c-type cytochromes. Acts by transferring electrons from cytoplasmic thioredoxin to the periplasm. This transfer involves a cascade of disulfide bond formation and reduction steps By similarity.
Catalytic activity	Protein dithiol + NAD(P)+ = protein disulfide + NAD(P)H. HAMAP MF_00399
Subcellular location	Cell inner membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the thioredoxin family. DsbD subfamily. Contains 1 thioredoxin domain.

Ontologies

Keywords
Biological process	Cytochrome c-type biogenesis Electron transport Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Redox-active center Signal Transmembrane
Ligand	NAD
Molecular function	Oxidoreductase
PTM	Disulfide bond
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell redox homeostasis Inferred from electronic annotation. Source: InterPro cytochrome complex assembly Inferred from electronic annotation. Source: HAMAP electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	electron carrier activity Inferred from electronic annotation. Source: HAMAP protein-disulfide reductase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	Potential
	Chain	20 – 569	550	Thiol:disulfide interchange protein dsbD HAMAP MF_00399		PRO_1000049607
Regions
	Transmembrane	168 – 188	21	Potential
	Transmembrane	213 – 233	21	Potential
	Transmembrane	248 – 268	21	Potential
	Transmembrane	301 – 321	21	Potential
	Transmembrane	328 – 348	21	Potential
	Transmembrane	362 – 382	21	Potential
	Transmembrane	391 – 411	21	Potential
	Domain	430 – 569	140	Thioredoxin
Amino acid modifications
	Disulfide bond	122 ↔ 128		Redox-active By similarity
	Disulfide bond	187 ↔ 309		Redox-active By similarity
	Disulfide bond	484 ↔ 487		Redox-active By similarity

Sequences

	Sequence		Length	Mass (Da)
	A8AMR4-1 [UniParc]. Last modified October 23, 2007. Version 1. Checksum: A4D4AC331EC56094	FASTA	569	61,756
	10 20 30 40 50 60 MAQRILTLIL LLCSTSAFAG LFDAPGRSQF VPADQAFSFD FQQNQHDLNL SWQVKDGYYL 70 80 90 100 110 120 YRKQISITPS QAEIAEVRLP AGVWHEDEFY GKSEIYRKRL NIPLIVNQAA SGATLTVTYQ 130 140 150 160 170 180 GCADAGFCYP PETKTVPLSE VSASTVAKST PSPVAAQTEE TPQPAARLPF SALWALLIGI 190 200 210 220 230 240 GIAFTPCVLP MYPLISGIVL GGKQRLSTGR ALLLTFIYVQ GMALTYTALG LVVAAAGLQF 250 260 270 280 290 300 QAALQHPYVL IGLALVFTLL ALSMFGLFTL QLPSSLQTRL TLMSNRQQGG SPGGVFVMGA 310 320 330 340 350 360 IAGLICSPCT TAPLSAILLY IAQSGNMWLG GGTLYLYALG MGLPLILITV FGNRLLPKSG 370 380 390 400 410 420 PWMEHVKTAF GFVILALPVF LLERVIGDEW GLRLWSLLGV AFFGWAFITS LHARRSGMRI 430 440 450 460 470 480 VQIILLAAAL VSVRPLQDWA FGATTAQTQA HLNFKPITTV DALNQALAEA KGKPIMLDLY 490 500 510 520 530 540 ADWCVACKEF EKYTFSDPQV QQTLGDTVLL QANVTANNAQ DVALLRHLNV LGLPTILFFD 550 560 AQGHEHPNAR VTGFMDATTF SAHLRDRQP

References

[1]	McClelland M., Sanderson E.K., Porwollik S., Spieth J., Clifton W.S., Latreille P., Courtney L., Wang C., Pepin K., Bhonagiri V., Nash W., Johnson M., Thiruvilangam P., Wilson R. Submitted (AUG-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000822 Genomic DNA. Translation: ABV14777.1.
RefSeq	YP_001455213.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5583492.
GenomeReviews	Gene locus CKO_03700 in contig CP000822_GR.
KEGG	cko:CKO_03700.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	A8AMR4. TITHILW.
Family and domain databases
HAMAP	MF_00399. [Tree]
InterPro	IPR003834. Cyt_c_assmbl_TM. IPR017936. Thioredoxin-like. IPR017937. Thioredoxin_CS. IPR012335. Thioredoxin_fold. [Graphical view]
Gene3D	G3DSA:3.40.30.10. Thioredoxin_fold. 1 hit.
Pfam	PF02683. DsbD. 1 hit. [Graphical view]
PROSITE	PS00194. THIOREDOXIN_1. 1 hit. PS51352. THIOREDOXIN_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DSBD_CITK8

Accession

Primary (citable) accession number: A8AMR4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 23, 2007
Last modified:	June 16, 2009
This is version 16 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)