Reviewed, UniProtKB/Swiss-Prot A8ALV4 (KHSE_CITK8)

Names and origin

Protein names

Recommended name:
    Homoserine kinase
      Short name=HSK
      Short name=HK
    EC=2.7.1.39

Gene names

Name:	thrB
Ordered Locus Names:	CKO_03384

Organism

Citrobacter koseri (strain ATCC BAA-895 / CDC 4225-83 / SGSC4696) [Complete proteome] [HAMAP]

Taxonomic identifier

290338 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Citrobacter

Protein attributes

Sequence length	309 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-homoserine = ADP + O-phospho-L-homoserine. HAMAP MF_00384
Pathway	Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 4/5. HAMAP MF_00384
Subcellular location	Cytoplasm Potential HAMAP MF_00384.
Sequence similarities	Belongs to the GHMP kinase family. Homoserine kinase subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Threonine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phosphorylation Inferred from electronic annotation. Source: InterPro threonine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP homoserine kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 309	309	Homoserine kinase HAMAP MF_00384		PRO_1000049121
Regions
	Nucleotide binding	91 – 101	11	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	A8ALV4-1 [UniParc]. Last modified October 23, 2007. Version 1. Checksum: 944334AF8FA1412C	FASTA	309	33,117
	10 20 30 40 50 60 MVKVYAPASS ANMSVGFDVL GAAVTPVDGS LLGDVVSVDA AESFTLNNLG RFADKLPSAP 70 80 90 100 110 120 RENIVYQCWE RFCQELGKQI PVAMTLEKNM PIGSGLGSSA CSVVAALVAM NEFCGKPLND 130 140 150 160 170 180 TRLLAIMGEL EGRISGSIHY DNVAPCFLGG MQLMIEENGI ISQQVPGFDE WLWVLAYPGI 190 200 210 220 230 240 NVSTAEARAI LPAQYRRQDC IAHGRHLAGF IHACYSRQPQ LAAKLMKDVI AEPYRARLLP 250 260 270 280 290 300 GFSQARQAVA EIGALASGIS GSGPTLFALC DKPDTAQRVA DWLGKNYLQN QEGFVHICQL DTAGARVLG

References

[1]	McClelland M., Sanderson E.K., Porwollik S., Spieth J., Clifton W.S., Latreille P., Courtney L., Wang C., Pepin K., Bhonagiri V., Nash W., Johnson M., Thiruvilangam P., Wilson R. Submitted (AUG-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000822 Genomic DNA. Translation: ABV14467.1.
RefSeq	YP_001454903.1.
3D structure databases
SMR	A8ALV4. Positions 2-308.
ModBase	Search...
Protein-protein interaction databases
STRING	A8ALV4.
Genome annotation databases
GeneID	5581943.
GenomeReviews	Gene locus CKO_03384 in contig CP000822_GR.
KEGG	cko:CKO_03384.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0083.
HOGENOM	HBG646290.
OMA	CGSAHAD.
Family and domain databases
HAMAP	MF_00384. Homoser_kinase. [Tree]
InterPro	IPR006204. GHMP_kinase. IPR013750. GHMP_kinase_C. IPR006203. GHMP_knse_ATP_bd_CS. IPR000870. Homoserine_kin. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
Pfam	PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF000676. Homoser_kin. 1 hit.
PRINTS	PR00958. HOMSERKINASE.
TIGRFAMs	TIGR00191. thrB. 1 hit.
PROSITE	PS00627. GHMP_KINASES_ATP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KHSE_CITK8

Accession

Primary (citable) accession number: A8ALV4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 23, 2007
Last modified:	February 9, 2010
This is version 18 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)