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A8AL13

- PFKA_CITK8

UniProt

A8AL13 - PFKA_CITK8

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Protein
ATP-dependent 6-phosphofructokinase
Gene
pfkA, CKO_03085
Organism
Citrobacter koseri (strain ATCC BAA-895 / CDC 4225-83 / SGSC4696)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis By similarity.UniRule annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Magnesium By similarity.UniRule annotation

Enzyme regulationi

Allosterically activated by ADP and other diphosphonucleosides, and allosterically inhibited by phosphoenolpyruvate By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei12 – 121ATP; via amide nitrogen By similarity
Metal bindingi104 – 1041Magnesium; catalytic By similarity
Active sitei128 – 1281Proton acceptor By similarity
Binding sitei155 – 1551Allosteric activator ADP By similarity
Binding sitei163 – 1631Substrate; shared with dimeric partner By similarity
Binding sitei212 – 2121Allosteric activator ADP By similarity
Binding sitei223 – 2231Substrate By similarity
Binding sitei244 – 2441Substrate; shared with dimeric partner By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi73 – 742ATP By similarity
Nucleotide bindingi103 – 1064ATP By similarity

GO - Molecular functioni

  1. 6-phosphofructokinase activity Source: UniProtKB-HAMAP
  2. ATP binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. fructose 6-phosphate metabolic process Source: InterPro
  2. glycolytic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciCKOS290338:GJ8L-3078-MONOMER.
UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinase (EC:2.7.1.11)
Short name:
ATP-PFK
Short name:
Phosphofructokinase
Alternative name(s):
Phosphohexokinase
Gene namesi
Name:pfkA
Ordered Locus Names:CKO_03085
OrganismiCitrobacter koseri (strain ATCC BAA-895 / CDC 4225-83 / SGSC4696)
Taxonomic identifieri290338 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeCitrobacter
ProteomesiUP000008148: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. 6-phosphofructokinase complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 320320ATP-dependent 6-phosphofructokinaseUniRule annotation
PRO_1000059750Add
BLAST

Proteomic databases

PRIDEiA8AL13.

Interactioni

Subunit structurei

Homotetramer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi290338.CKO_03085.

Structurei

3D structure databases

ProteinModelPortaliA8AL13.
SMRiA8AL13. Positions 1-320.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni22 – 265Allosteric activator ADP binding; shared with dimeric partner By similarity
Regioni55 – 606Allosteric activator ADP binding; shared with dimeric partner By similarity
Regioni126 – 1283Substrate binding By similarity
Regioni170 – 1723Substrate binding By similarity
Regioni186 – 1883Allosteric activator ADP binding By similarity
Regioni214 – 2163Allosteric activator ADP binding By similarity
Regioni250 – 2534Substrate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0205.
HOGENOMiHOG000248870.
KOiK00850.
OMAiGFGGRCV.
OrthoDBiEOG644ZRM.

Family and domain databases

HAMAPiMF_00339. Phosphofructokinase_I_B1.
InterProiIPR012003. ATP_PFK_prok.
IPR012828. PFKA_ATP.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
TIGRFAMsiTIGR02482. PFKA_ATP. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A8AL13-1 [UniParc]FASTAAdd to Basket

« Hide

MIKKIGVLTS GGDAPGMNAA IRGVVRAALT EGLEVMGIYD GYLGLYEDRM    50
VQLDRYSVSD MINRGGTFLG SARFPEFRDE NVRAVAIENL KKRGIDALVV 100
IGGDGSYMGA KRLTEMGFPC IGLPGTIDND IKGTDYTIGY FTALGTVVEA 150
IDRLRDTSSS HQRISIVEVM GRYCGDLTLA AAIAGGCEFV VVPEVEFSRD 200
DLVAEIKAGI AKGKKHAIVA ITEHMCDVDE LAHYIEKETG RETRATVLGH 250
IQRGGSPVPY DRILASRMGS YAIELLLEGF GGRCVGIQNE QLVHHDIIDA 300
IENMKRPFKG DWLDCAKKLY 320
Length:320
Mass (Da):34,840
Last modified:October 23, 2007 - v1
Checksum:i6D64E93EA0DD683D
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000822 Genomic DNA. Translation: ABV14176.1.
RefSeqiYP_001454612.1. NC_009792.1.

Genome annotation databases

EnsemblBacteriaiABV14176; ABV14176; CKO_03085.
GeneIDi5584690.
KEGGicko:CKO_03085.
PATRICi20388794. VBICitKos71230_2580.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000822 Genomic DNA. Translation: ABV14176.1 .
RefSeqi YP_001454612.1. NC_009792.1.

3D structure databases

ProteinModelPortali A8AL13.
SMRi A8AL13. Positions 1-320.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 290338.CKO_03085.

Proteomic databases

PRIDEi A8AL13.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABV14176 ; ABV14176 ; CKO_03085 .
GeneIDi 5584690.
KEGGi cko:CKO_03085.
PATRICi 20388794. VBICitKos71230_2580.

Phylogenomic databases

eggNOGi COG0205.
HOGENOMi HOG000248870.
KOi K00850.
OMAi GFGGRCV.
OrthoDBi EOG644ZRM.

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00182 .
BioCyci CKOS290338:GJ8L-3078-MONOMER.

Family and domain databases

HAMAPi MF_00339. Phosphofructokinase_I_B1.
InterProi IPR012003. ATP_PFK_prok.
IPR012828. PFKA_ATP.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view ]
Pfami PF00365. PFK. 1 hit.
[Graphical view ]
PIRSFi PIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSi PR00476. PHFRCTKINASE.
SUPFAMi SSF53784. SSF53784. 1 hit.
TIGRFAMsi TIGR02482. PFKA_ATP. 1 hit.
PROSITEi PS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-895 / CDC 4225-83 / SGSC4696.

Entry informationi

Entry nameiPFKA_CITK8
AccessioniPrimary (citable) accession number: A8AL13
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: October 23, 2007
Last modified: July 9, 2014
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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