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Reviewed, UniProtKB/Swiss-Prot A8AKB8 (BGAL_CITK8)

Last modified November 3, 2009. Version 14. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Beta-galactosidase
      Short name=Beta-gal
    EC=3.2.1.23
Alternative name(s):
    Lactase
Gene names
Name: lacZ
Ordered Locus Names: CKO_02825
OrganismCitrobacter koseri (strain ATCC BAA-895 / CDC 4225-83 / SGSC4696) [Complete proteome] [HAMAP]
Taxonomic identifier290338 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Citrobacter

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Protein attributes

Sequence length1025 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceInferred from homology.

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General annotation (Comments)

Catalytic activity

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides. HAMAP MF_01687

Cofactor

Binds 2 magnesium ions per monomer By similarity.

Binds 1 sodium ion per monomer By similarity.

Subunit structure

Homotetramer By similarity.

Sequence similarities

Belongs to the glycosyl hydrolase 2 family.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10251025Beta-galactosidase HAMAP MF_01687
PRO_0000366981

Regions

Region538 – 5414Substrate binding By similarity

Sites

Active site4621Proton donor By similarity
Active site5381Nucleophile By similarity
Metal binding2021Sodium By similarity
Metal binding4171Magnesium 1 By similarity
Metal binding4191Magnesium 1 By similarity
Metal binding4621Magnesium 1 By similarity
Metal binding5981Magnesium 2 By similarity
Metal binding6021Sodium; via carbonyl oxygen By similarity
Metal binding6051Sodium By similarity
Binding site1031Substrate By similarity
Binding site2021Substrate By similarity
Binding site4621Substrate By similarity
Binding site6051Substrate By similarity
Binding site10031Substrate By similarity
Site3581Transition state stabilizer By similarity
Site3921Transition state stabilizer By similarity

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Sequences

Sequence LengthMass (Da)Tools
A8AKB8-1 [UniParc].

Last modified October 23, 2007. Version 1.
Checksum: 446901FFBF2DEC14

FASTA1,025116,279
        10         20         30         40         50         60 
MPQNADSLAV VLKCRDWENP GVTQLNRLEA HPPFCSWRNA DDARVNRDSA QKRSLNGEWT 

        70         80         90        100        110        120 
FAWFSAPEAV PESWRTSDLQ QADSVRVPSN WQMDGYDAPI YTNVTYPIPV NPPFVPADNP 

       130        140        150        160        170        180 
TGCYSLTFSI DADWLQAGQT RIIFDGVNSA FHLWCNSRWV GYGQDSRLPS EFDLTHFLLK 

       190        200        210        220        230        240 
GENRLAVMVL RWSDGSYLED QDMWRMSGIF RDVSLLHKPA TQIRDLRINT RFNDDFSRAV 

       250        260        270        280        290        300 
LEAEVRTTGE RRDDLRVTVQ LWDSETFVGE KTAPLGSEII DERGAYPDRT TLRLNVEHPA 

       310        320        330        340        350        360 
LWSAETPHLY RAVVQLHAAD GTLIEAEACD VGFRQVSIEN GLLLLNGKPL LIRGANRHEH 

       370        380        390        400        410        420 
HPENGQVMDK ETMIKDILLM KQNNFNAVRC SHYPNHPLWY TLCDRYGLYV VDEANIETHG 

       430        440        450        460        470        480 
MVPMNRLSDD PVWLPAMSQR VTRMVQRDRN HPSIIIWSLG NESGHGANHD ALYRWIKSED 

       490        500        510        520        530        540 
PSRPVQYEGG GANTAATDII CPMYARVDQD QPFPAVPKWS IKKWLSMPGE QRPLILCEYA 

       550        560        570        580        590        600 
HAMGNSLGGY AKYWQAFRQY PRLQGGFVWD WVDQSLIKYD DDGRPWSAYG GDFGDAPNDR 

       610        620        630        640        650        660 
QFCMNGLVFA DRTPHPSLYE AKHAQQFFQF ALLPGAECQI EVTSEYLFRH SDNEVLHWSL 

       670        680        690        700        710        720 
ALDGNPLTAG VVTLDIPPQG RQIIALPALP EAETAGQLWL TVRVEQPQAT AWSQAGHISA 

       730        740        750        760        770        780 
WQQWKLGEKL ATQWPQHAGN APQLTSSGTA FRIVAGEKRW EFSRQQGVLT QFWIGEEAQL 

       790        800        810        820        830        840 
LTPLVDQFTR APLDNDIGVS EATRIDPNAW VERWKAAGHY QAKAVLLQCD ADTLASAVLI 

       850        860        870        880        890        900 
TTAHAWQYQG ETLFISRKTY RIDGNGEMQI TVDVDVASGT PHPARIGLSC QLAQVAERVN 

       910        920        930        940        950        960 
WLGLGPHENY PDRLSAACFE RWDLPLEEMY TPYVFPSENG LRCGTRELLY GAHQWRGDFQ 

       970        980        990       1000       1010       1020 
FNISRYGQKQ LMETSHRHLL QPEAGTWLNI DGFHMGVGGD DSWSPSVSAE YQLSAGRYHY 


QISWR

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References

[1]McClelland M., Sanderson E.K., Porwollik S., Spieth J., Clifton W.S., Latreille P., Courtney L., Wang C., Pepin K., Bhonagiri V., Nash W., Johnson M., Thiruvilangam P., Wilson R.
Submitted (AUG-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

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Cross-references

Sequence databases

CP000822 Genomic DNA. Translation: ABV13931.1.
RefSeqYP_001454367.1.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

STRINGA8AKB8.

Genome annotation databases

GeneID5581703.
GenomeReviewsGene locus CKO_02825 in contig CP000822_GR.
KEGGcko:CKO_02825.

Organism-specific databases

CMRSearch...

Phylogenomic databases

OMANELLHWM.

Family and domain databases

HAMAPMF_01687.
[Tree]
InterProIPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR006104. Glyco_hydro_2_carb-bd.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006103. Glyco_hydro_2_TIM.
IPR004199. Glyco_hydro_42_D5.
IPR013781. Glyco_hydro_sg_catalytic.
[Graphical view]
Gene3DG3DSA:2.60.40.320. Glyco_hydro_2/20_Ig-like. 2 hits.
G3DSA:2.70.98.10. Glyco_hydro_42_D5. 1 hit.
G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
PfamPF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSPR00132. GLHYDRLASE2.
PROSITEPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameBGAL_CITK8
AccessionPrimary (citable) accession number: A8AKB8
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: October 23, 2007
Last modified: November 3, 2009
This is version 14 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents