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A8AKB8 (BGAL_CITK8) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 41. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-galactosidase

Short name=Beta-gal
EC=3.2.1.23
Alternative name(s):
Lactase
Gene names
Name:lacZ
Ordered Locus Names:CKO_02825
OrganismCitrobacter koseri (strain ATCC BAA-895 / CDC 4225-83 / SGSC4696) [Complete proteome] [HAMAP]
Taxonomic identifier290338 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeCitrobacter

Protein attributes

Sequence length1025 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides. HAMAP-Rule MF_01687

Cofactor

Binds 2 magnesium ions per monomer By similarity. HAMAP-Rule MF_01687

Binds 1 sodium ion per monomer By similarity. HAMAP-Rule MF_01687

Subunit structure

Homotetramer By similarity. HAMAP-Rule MF_01687

Sequence similarities

Belongs to the glycosyl hydrolase 2 family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10251025Beta-galactosidase HAMAP-Rule MF_01687
PRO_0000366981

Regions

Region538 – 5414Substrate binding By similarity

Sites

Active site4621Proton donor By similarity
Active site5381Nucleophile By similarity
Metal binding2021Sodium By similarity
Metal binding4171Magnesium 1 By similarity
Metal binding4191Magnesium 1 By similarity
Metal binding4621Magnesium 1 By similarity
Metal binding5981Magnesium 2 By similarity
Metal binding6021Sodium; via carbonyl oxygen By similarity
Metal binding6051Sodium By similarity
Binding site1031Substrate By similarity
Binding site2021Substrate By similarity
Binding site4621Substrate By similarity
Binding site6051Substrate By similarity
Binding site10031Substrate By similarity
Site3581Transition state stabilizer By similarity
Site3921Transition state stabilizer By similarity

Sequences

Sequence LengthMass (Da)Tools
A8AKB8 [UniParc].

Last modified October 23, 2007. Version 1.
Checksum: 446901FFBF2DEC14

FASTA1,025116,279
        10         20         30         40         50         60 
MPQNADSLAV VLKCRDWENP GVTQLNRLEA HPPFCSWRNA DDARVNRDSA QKRSLNGEWT 

        70         80         90        100        110        120 
FAWFSAPEAV PESWRTSDLQ QADSVRVPSN WQMDGYDAPI YTNVTYPIPV NPPFVPADNP 

       130        140        150        160        170        180 
TGCYSLTFSI DADWLQAGQT RIIFDGVNSA FHLWCNSRWV GYGQDSRLPS EFDLTHFLLK 

       190        200        210        220        230        240 
GENRLAVMVL RWSDGSYLED QDMWRMSGIF RDVSLLHKPA TQIRDLRINT RFNDDFSRAV 

       250        260        270        280        290        300 
LEAEVRTTGE RRDDLRVTVQ LWDSETFVGE KTAPLGSEII DERGAYPDRT TLRLNVEHPA 

       310        320        330        340        350        360 
LWSAETPHLY RAVVQLHAAD GTLIEAEACD VGFRQVSIEN GLLLLNGKPL LIRGANRHEH 

       370        380        390        400        410        420 
HPENGQVMDK ETMIKDILLM KQNNFNAVRC SHYPNHPLWY TLCDRYGLYV VDEANIETHG 

       430        440        450        460        470        480 
MVPMNRLSDD PVWLPAMSQR VTRMVQRDRN HPSIIIWSLG NESGHGANHD ALYRWIKSED 

       490        500        510        520        530        540 
PSRPVQYEGG GANTAATDII CPMYARVDQD QPFPAVPKWS IKKWLSMPGE QRPLILCEYA 

       550        560        570        580        590        600 
HAMGNSLGGY AKYWQAFRQY PRLQGGFVWD WVDQSLIKYD DDGRPWSAYG GDFGDAPNDR 

       610        620        630        640        650        660 
QFCMNGLVFA DRTPHPSLYE AKHAQQFFQF ALLPGAECQI EVTSEYLFRH SDNEVLHWSL 

       670        680        690        700        710        720 
ALDGNPLTAG VVTLDIPPQG RQIIALPALP EAETAGQLWL TVRVEQPQAT AWSQAGHISA 

       730        740        750        760        770        780 
WQQWKLGEKL ATQWPQHAGN APQLTSSGTA FRIVAGEKRW EFSRQQGVLT QFWIGEEAQL 

       790        800        810        820        830        840 
LTPLVDQFTR APLDNDIGVS EATRIDPNAW VERWKAAGHY QAKAVLLQCD ADTLASAVLI 

       850        860        870        880        890        900 
TTAHAWQYQG ETLFISRKTY RIDGNGEMQI TVDVDVASGT PHPARIGLSC QLAQVAERVN 

       910        920        930        940        950        960 
WLGLGPHENY PDRLSAACFE RWDLPLEEMY TPYVFPSENG LRCGTRELLY GAHQWRGDFQ 

       970        980        990       1000       1010       1020 
FNISRYGQKQ LMETSHRHLL QPEAGTWLNI DGFHMGVGGD DSWSPSVSAE YQLSAGRYHY 


QISWR 

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References

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000822 Genomic DNA. Translation: ABV13931.1.
RefSeqYP_001454367.1. NC_009792.1.

3D structure databases

ProteinModelPortalA8AKB8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING290338.CKO_02825.

Protein family/group databases

CAZyGH2. Glycoside Hydrolase Family 2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABV13931; ABV13931; CKO_02825.
GeneID5581703.
KEGGcko:CKO_02825.
PATRIC20388327. VBICitKos71230_2363.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG3250.
HOGENOMHOG000252443.
KOK01190.
OMAHRCAWDQ.
OrthoDBEOG6XWV0T.
ProtClustDBPRK09525.

Enzyme and pathway databases

BioCycCKOS290338:GJ8L-2818-MONOMER.

Family and domain databases

Gene3D2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPMF_01687. Beta_gal.
InterProIPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamPF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSPR00132. GLHYDRLASE2.
SMARTSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMSSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameBGAL_CITK8
AccessionPrimary (citable) accession number: A8AKB8
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: October 23, 2007
Last modified: February 19, 2014
This is version 41 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries