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A8AKB8

- BGAL_CITK8

UniProt

A8AKB8 - BGAL_CITK8

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Protein
Beta-galactosidase
Gene
lacZ, CKO_02825
Organism
Citrobacter koseri (strain ATCC BAA-895 / CDC 4225-83 / SGSC4696)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.UniRule annotation

Cofactori

Binds 2 magnesium ions per monomer By similarity.UniRule annotation
Binds 1 sodium ion per monomer By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei103 – 1031Substrate By similarity
Metal bindingi202 – 2021Sodium By similarity
Binding sitei202 – 2021Substrate By similarity
Sitei358 – 3581Transition state stabilizer By similarity
Sitei392 – 3921Transition state stabilizer By similarity
Metal bindingi417 – 4171Magnesium 1 By similarity
Metal bindingi419 – 4191Magnesium 1 By similarity
Active sitei462 – 4621Proton donor By similarity
Metal bindingi462 – 4621Magnesium 1 By similarity
Binding sitei462 – 4621Substrate By similarity
Active sitei538 – 5381Nucleophile By similarity
Metal bindingi598 – 5981Magnesium 2 By similarity
Metal bindingi602 – 6021Sodium; via carbonyl oxygen By similarity
Metal bindingi605 – 6051Sodium By similarity
Binding sitei605 – 6051Substrate By similarity
Binding sitei1003 – 10031Substrate By similarity

GO - Molecular functioni

  1. beta-galactosidase activity Source: UniProtKB-EC
  2. carbohydrate binding Source: InterPro
  3. magnesium ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding, Sodium

Enzyme and pathway databases

BioCyciCKOS290338:GJ8L-2818-MONOMER.

Protein family/group databases

CAZyiGH2. Glycoside Hydrolase Family 2.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidase (EC:3.2.1.23)
Short name:
Beta-gal
Alternative name(s):
Lactase
Gene namesi
Name:lacZ
Ordered Locus Names:CKO_02825
OrganismiCitrobacter koseri (strain ATCC BAA-895 / CDC 4225-83 / SGSC4696)
Taxonomic identifieri290338 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeCitrobacter
ProteomesiUP000008148: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. beta-galactosidase complex Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10251025Beta-galactosidaseUniRule annotation
PRO_0000366981Add
BLAST

Interactioni

Subunit structurei

Homotetramer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi290338.CKO_02825.

Structurei

3D structure databases

ProteinModelPortaliA8AKB8.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni538 – 5414Substrate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG3250.
HOGENOMiHOG000252443.
KOiK01190.
OMAiDPKKPYI.
OrthoDBiEOG6XWV0T.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_01687. Beta_gal.
InterProiIPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSiPR00132. GLHYDRLASE2.
SMARTiSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMiSSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A8AKB8-1 [UniParc]FASTAAdd to Basket

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MPQNADSLAV VLKCRDWENP GVTQLNRLEA HPPFCSWRNA DDARVNRDSA     50
QKRSLNGEWT FAWFSAPEAV PESWRTSDLQ QADSVRVPSN WQMDGYDAPI 100
YTNVTYPIPV NPPFVPADNP TGCYSLTFSI DADWLQAGQT RIIFDGVNSA 150
FHLWCNSRWV GYGQDSRLPS EFDLTHFLLK GENRLAVMVL RWSDGSYLED 200
QDMWRMSGIF RDVSLLHKPA TQIRDLRINT RFNDDFSRAV LEAEVRTTGE 250
RRDDLRVTVQ LWDSETFVGE KTAPLGSEII DERGAYPDRT TLRLNVEHPA 300
LWSAETPHLY RAVVQLHAAD GTLIEAEACD VGFRQVSIEN GLLLLNGKPL 350
LIRGANRHEH HPENGQVMDK ETMIKDILLM KQNNFNAVRC SHYPNHPLWY 400
TLCDRYGLYV VDEANIETHG MVPMNRLSDD PVWLPAMSQR VTRMVQRDRN 450
HPSIIIWSLG NESGHGANHD ALYRWIKSED PSRPVQYEGG GANTAATDII 500
CPMYARVDQD QPFPAVPKWS IKKWLSMPGE QRPLILCEYA HAMGNSLGGY 550
AKYWQAFRQY PRLQGGFVWD WVDQSLIKYD DDGRPWSAYG GDFGDAPNDR 600
QFCMNGLVFA DRTPHPSLYE AKHAQQFFQF ALLPGAECQI EVTSEYLFRH 650
SDNEVLHWSL ALDGNPLTAG VVTLDIPPQG RQIIALPALP EAETAGQLWL 700
TVRVEQPQAT AWSQAGHISA WQQWKLGEKL ATQWPQHAGN APQLTSSGTA 750
FRIVAGEKRW EFSRQQGVLT QFWIGEEAQL LTPLVDQFTR APLDNDIGVS 800
EATRIDPNAW VERWKAAGHY QAKAVLLQCD ADTLASAVLI TTAHAWQYQG 850
ETLFISRKTY RIDGNGEMQI TVDVDVASGT PHPARIGLSC QLAQVAERVN 900
WLGLGPHENY PDRLSAACFE RWDLPLEEMY TPYVFPSENG LRCGTRELLY 950
GAHQWRGDFQ FNISRYGQKQ LMETSHRHLL QPEAGTWLNI DGFHMGVGGD 1000
DSWSPSVSAE YQLSAGRYHY QISWR 1025
Length:1,025
Mass (Da):116,279
Last modified:October 23, 2007 - v1
Checksum:i446901FFBF2DEC14
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000822 Genomic DNA. Translation: ABV13931.1.
RefSeqiWP_012133644.1. NC_009792.1.
YP_001454367.1. NC_009792.1.

Genome annotation databases

EnsemblBacteriaiABV13931; ABV13931; CKO_02825.
GeneIDi5581703.
KEGGicko:CKO_02825.
PATRICi20388327. VBICitKos71230_2363.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000822 Genomic DNA. Translation: ABV13931.1 .
RefSeqi WP_012133644.1. NC_009792.1.
YP_001454367.1. NC_009792.1.

3D structure databases

ProteinModelPortali A8AKB8.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 290338.CKO_02825.

Protein family/group databases

CAZyi GH2. Glycoside Hydrolase Family 2.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABV13931 ; ABV13931 ; CKO_02825 .
GeneIDi 5581703.
KEGGi cko:CKO_02825.
PATRICi 20388327. VBICitKos71230_2363.

Phylogenomic databases

eggNOGi COG3250.
HOGENOMi HOG000252443.
KOi K01190.
OMAi DPKKPYI.
OrthoDBi EOG6XWV0T.

Enzyme and pathway databases

BioCyci CKOS290338:GJ8L-2818-MONOMER.

Family and domain databases

Gene3Di 2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPi MF_01687. Beta_gal.
InterProi IPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view ]
PRINTSi PR00132. GLHYDRLASE2.
SMARTi SM01038. Bgal_small_N. 1 hit.
[Graphical view ]
SUPFAMi SSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEi PS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-895 / CDC 4225-83 / SGSC4696.

Entry informationi

Entry nameiBGAL_CITK8
AccessioniPrimary (citable) accession number: A8AKB8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: October 23, 2007
Last modified: September 3, 2014
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi