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Protein

Beta-galactosidase

Gene

lacZ

Organism
Citrobacter koseri (strain ATCC BAA-895 / CDC 4225-83 / SGSC4696)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • Mg2+UniRule annotationNote: Binds 2 magnesium ions per monomer.UniRule annotation
  • Na+UniRule annotationNote: Binds 1 sodium ion per monomer.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei103SubstrateUniRule annotation1
Metal bindingi202SodiumUniRule annotation1
Binding sitei202SubstrateUniRule annotation1
Sitei358Transition state stabilizerUniRule annotation1
Sitei392Transition state stabilizerUniRule annotation1
Metal bindingi417Magnesium 1UniRule annotation1
Metal bindingi419Magnesium 1UniRule annotation1
Active sitei462Proton donorUniRule annotation1
Metal bindingi462Magnesium 1UniRule annotation1
Binding sitei462SubstrateUniRule annotation1
Active sitei538NucleophileUniRule annotation1
Metal bindingi598Magnesium 2UniRule annotation1
Metal bindingi602Sodium; via carbonyl oxygenUniRule annotation1
Metal bindingi605SodiumUniRule annotation1
Binding sitei605SubstrateUniRule annotation1
Binding sitei1003SubstrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding, Sodium

Protein family/group databases

CAZyiGH2. Glycoside Hydrolase Family 2.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidaseUniRule annotation (EC:3.2.1.23UniRule annotation)
Short name:
Beta-galUniRule annotation
Alternative name(s):
LactaseUniRule annotation
Gene namesi
Name:lacZUniRule annotation
Ordered Locus Names:CKO_02825
OrganismiCitrobacter koseri (strain ATCC BAA-895 / CDC 4225-83 / SGSC4696)
Taxonomic identifieri290338 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeCitrobacter
Proteomesi
  • UP000008148 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003669811 – 1025Beta-galactosidaseAdd BLAST1025

Proteomic databases

PRIDEiA8AKB8.

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi290338.CKO_02825.

Structurei

3D structure databases

ProteinModelPortaliA8AKB8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni538 – 541Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the glycosyl hydrolase 2 family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CNT. Bacteria.
COG3250. LUCA.
HOGENOMiHOG000252443.
KOiK01190.
OMAiCLDGLLF.
OrthoDBiPOG091H07QB.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_01687. Beta_gal. 1 hit.
InterProiIPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. GH-type_carb-bd.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR006103. Glyco_hydro_2_cat.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR032312. LacZ_4.
[Graphical view]
PfamiPF02929. Bgal_small_N. 1 hit.
PF16353. DUF4981. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSiPR00132. GLHYDRLASE2.
SMARTiSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMiSSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A8AKB8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPQNADSLAV VLKCRDWENP GVTQLNRLEA HPPFCSWRNA DDARVNRDSA
60 70 80 90 100
QKRSLNGEWT FAWFSAPEAV PESWRTSDLQ QADSVRVPSN WQMDGYDAPI
110 120 130 140 150
YTNVTYPIPV NPPFVPADNP TGCYSLTFSI DADWLQAGQT RIIFDGVNSA
160 170 180 190 200
FHLWCNSRWV GYGQDSRLPS EFDLTHFLLK GENRLAVMVL RWSDGSYLED
210 220 230 240 250
QDMWRMSGIF RDVSLLHKPA TQIRDLRINT RFNDDFSRAV LEAEVRTTGE
260 270 280 290 300
RRDDLRVTVQ LWDSETFVGE KTAPLGSEII DERGAYPDRT TLRLNVEHPA
310 320 330 340 350
LWSAETPHLY RAVVQLHAAD GTLIEAEACD VGFRQVSIEN GLLLLNGKPL
360 370 380 390 400
LIRGANRHEH HPENGQVMDK ETMIKDILLM KQNNFNAVRC SHYPNHPLWY
410 420 430 440 450
TLCDRYGLYV VDEANIETHG MVPMNRLSDD PVWLPAMSQR VTRMVQRDRN
460 470 480 490 500
HPSIIIWSLG NESGHGANHD ALYRWIKSED PSRPVQYEGG GANTAATDII
510 520 530 540 550
CPMYARVDQD QPFPAVPKWS IKKWLSMPGE QRPLILCEYA HAMGNSLGGY
560 570 580 590 600
AKYWQAFRQY PRLQGGFVWD WVDQSLIKYD DDGRPWSAYG GDFGDAPNDR
610 620 630 640 650
QFCMNGLVFA DRTPHPSLYE AKHAQQFFQF ALLPGAECQI EVTSEYLFRH
660 670 680 690 700
SDNEVLHWSL ALDGNPLTAG VVTLDIPPQG RQIIALPALP EAETAGQLWL
710 720 730 740 750
TVRVEQPQAT AWSQAGHISA WQQWKLGEKL ATQWPQHAGN APQLTSSGTA
760 770 780 790 800
FRIVAGEKRW EFSRQQGVLT QFWIGEEAQL LTPLVDQFTR APLDNDIGVS
810 820 830 840 850
EATRIDPNAW VERWKAAGHY QAKAVLLQCD ADTLASAVLI TTAHAWQYQG
860 870 880 890 900
ETLFISRKTY RIDGNGEMQI TVDVDVASGT PHPARIGLSC QLAQVAERVN
910 920 930 940 950
WLGLGPHENY PDRLSAACFE RWDLPLEEMY TPYVFPSENG LRCGTRELLY
960 970 980 990 1000
GAHQWRGDFQ FNISRYGQKQ LMETSHRHLL QPEAGTWLNI DGFHMGVGGD
1010 1020
DSWSPSVSAE YQLSAGRYHY QISWR
Length:1,025
Mass (Da):116,279
Last modified:October 23, 2007 - v1
Checksum:i446901FFBF2DEC14
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000822 Genomic DNA. Translation: ABV13931.1.
RefSeqiWP_012133644.1. NC_009792.1.

Genome annotation databases

EnsemblBacteriaiABV13931; ABV13931; CKO_02825.
KEGGicko:CKO_02825.
PATRICi20388327. VBICitKos71230_2363.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000822 Genomic DNA. Translation: ABV13931.1.
RefSeqiWP_012133644.1. NC_009792.1.

3D structure databases

ProteinModelPortaliA8AKB8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi290338.CKO_02825.

Protein family/group databases

CAZyiGH2. Glycoside Hydrolase Family 2.

Proteomic databases

PRIDEiA8AKB8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABV13931; ABV13931; CKO_02825.
KEGGicko:CKO_02825.
PATRICi20388327. VBICitKos71230_2363.

Phylogenomic databases

eggNOGiENOG4105CNT. Bacteria.
COG3250. LUCA.
HOGENOMiHOG000252443.
KOiK01190.
OMAiCLDGLLF.
OrthoDBiPOG091H07QB.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_01687. Beta_gal. 1 hit.
InterProiIPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. GH-type_carb-bd.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR006103. Glyco_hydro_2_cat.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR032312. LacZ_4.
[Graphical view]
PfamiPF02929. Bgal_small_N. 1 hit.
PF16353. DUF4981. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSiPR00132. GLHYDRLASE2.
SMARTiSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMiSSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBGAL_CITK8
AccessioniPrimary (citable) accession number: A8AKB8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: October 23, 2007
Last modified: November 2, 2016
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.