Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

A8AKB8

- BGAL_CITK8

UniProt

A8AKB8 - BGAL_CITK8

Protein

Beta-galactosidase

Gene

lacZ

Organism
Citrobacter koseri (strain ATCC BAA-895 / CDC 4225-83 / SGSC4696)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 44 (01 Oct 2014)
      Sequence version 1 (23 Oct 2007)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalytic activityi

    Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.UniRule annotation

    Cofactori

    Binds 2 magnesium ions per monomer.UniRule annotation
    Binds 1 sodium ion per monomer.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei103 – 1031SubstrateUniRule annotation
    Metal bindingi202 – 2021SodiumUniRule annotation
    Binding sitei202 – 2021SubstrateUniRule annotation
    Sitei358 – 3581Transition state stabilizerUniRule annotation
    Sitei392 – 3921Transition state stabilizerUniRule annotation
    Metal bindingi417 – 4171Magnesium 1UniRule annotation
    Metal bindingi419 – 4191Magnesium 1UniRule annotation
    Active sitei462 – 4621Proton donorUniRule annotation
    Metal bindingi462 – 4621Magnesium 1UniRule annotation
    Binding sitei462 – 4621SubstrateUniRule annotation
    Active sitei538 – 5381NucleophileUniRule annotation
    Metal bindingi598 – 5981Magnesium 2UniRule annotation
    Metal bindingi602 – 6021Sodium; via carbonyl oxygenUniRule annotation
    Metal bindingi605 – 6051SodiumUniRule annotation
    Binding sitei605 – 6051SubstrateUniRule annotation
    Binding sitei1003 – 10031SubstrateUniRule annotation

    GO - Molecular functioni

    1. beta-galactosidase activity Source: UniProtKB-EC
    2. carbohydrate binding Source: InterPro
    3. magnesium ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Ligandi

    Magnesium, Metal-binding, Sodium

    Enzyme and pathway databases

    BioCyciCKOS290338:GJ8L-2818-MONOMER.

    Protein family/group databases

    CAZyiGH2. Glycoside Hydrolase Family 2.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-galactosidaseUniRule annotation (EC:3.2.1.23UniRule annotation)
    Short name:
    Beta-galUniRule annotation
    Alternative name(s):
    LactaseUniRule annotation
    Gene namesi
    Name:lacZUniRule annotation
    Ordered Locus Names:CKO_02825
    OrganismiCitrobacter koseri (strain ATCC BAA-895 / CDC 4225-83 / SGSC4696)
    Taxonomic identifieri290338 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeCitrobacter
    ProteomesiUP000008148: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. beta-galactosidase complex Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10251025Beta-galactosidasePRO_0000366981Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer.UniRule annotation

    Protein-protein interaction databases

    STRINGi290338.CKO_02825.

    Structurei

    3D structure databases

    ProteinModelPortaliA8AKB8.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni538 – 5414Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 2 family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG3250.
    HOGENOMiHOG000252443.
    KOiK01190.
    OMAiDPKKPYI.
    OrthoDBiEOG6XWV0T.

    Family and domain databases

    Gene3Di2.60.120.260. 1 hit.
    2.60.40.320. 2 hits.
    2.70.98.10. 1 hit.
    3.20.20.80. 1 hit.
    HAMAPiMF_01687. Beta_gal.
    InterProiIPR004199. B-gal_small/dom_5.
    IPR011013. Gal_mutarotase_SF_dom.
    IPR008979. Galactose-bd-like.
    IPR014718. Glyco_hydro-type_carb-bd_sub.
    IPR006101. Glyco_hydro_2.
    IPR013812. Glyco_hydro_2/20_Ig-like.
    IPR023232. Glyco_hydro_2_AS.
    IPR023933. Glyco_hydro_2_beta_Galsidase.
    IPR023230. Glyco_hydro_2_CS.
    IPR006102. Glyco_hydro_2_Ig-like.
    IPR006104. Glyco_hydro_2_N.
    IPR006103. Glyco_hydro_2_TIM.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF02929. Bgal_small_N. 1 hit.
    PF00703. Glyco_hydro_2. 1 hit.
    PF02836. Glyco_hydro_2_C. 1 hit.
    PF02837. Glyco_hydro_2_N. 1 hit.
    [Graphical view]
    PRINTSiPR00132. GLHYDRLASE2.
    SMARTiSM01038. Bgal_small_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF49303. SSF49303. 2 hits.
    SSF49785. SSF49785. 1 hit.
    SSF51445. SSF51445. 1 hit.
    SSF74650. SSF74650. 1 hit.
    PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
    PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A8AKB8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPQNADSLAV VLKCRDWENP GVTQLNRLEA HPPFCSWRNA DDARVNRDSA     50
    QKRSLNGEWT FAWFSAPEAV PESWRTSDLQ QADSVRVPSN WQMDGYDAPI 100
    YTNVTYPIPV NPPFVPADNP TGCYSLTFSI DADWLQAGQT RIIFDGVNSA 150
    FHLWCNSRWV GYGQDSRLPS EFDLTHFLLK GENRLAVMVL RWSDGSYLED 200
    QDMWRMSGIF RDVSLLHKPA TQIRDLRINT RFNDDFSRAV LEAEVRTTGE 250
    RRDDLRVTVQ LWDSETFVGE KTAPLGSEII DERGAYPDRT TLRLNVEHPA 300
    LWSAETPHLY RAVVQLHAAD GTLIEAEACD VGFRQVSIEN GLLLLNGKPL 350
    LIRGANRHEH HPENGQVMDK ETMIKDILLM KQNNFNAVRC SHYPNHPLWY 400
    TLCDRYGLYV VDEANIETHG MVPMNRLSDD PVWLPAMSQR VTRMVQRDRN 450
    HPSIIIWSLG NESGHGANHD ALYRWIKSED PSRPVQYEGG GANTAATDII 500
    CPMYARVDQD QPFPAVPKWS IKKWLSMPGE QRPLILCEYA HAMGNSLGGY 550
    AKYWQAFRQY PRLQGGFVWD WVDQSLIKYD DDGRPWSAYG GDFGDAPNDR 600
    QFCMNGLVFA DRTPHPSLYE AKHAQQFFQF ALLPGAECQI EVTSEYLFRH 650
    SDNEVLHWSL ALDGNPLTAG VVTLDIPPQG RQIIALPALP EAETAGQLWL 700
    TVRVEQPQAT AWSQAGHISA WQQWKLGEKL ATQWPQHAGN APQLTSSGTA 750
    FRIVAGEKRW EFSRQQGVLT QFWIGEEAQL LTPLVDQFTR APLDNDIGVS 800
    EATRIDPNAW VERWKAAGHY QAKAVLLQCD ADTLASAVLI TTAHAWQYQG 850
    ETLFISRKTY RIDGNGEMQI TVDVDVASGT PHPARIGLSC QLAQVAERVN 900
    WLGLGPHENY PDRLSAACFE RWDLPLEEMY TPYVFPSENG LRCGTRELLY 950
    GAHQWRGDFQ FNISRYGQKQ LMETSHRHLL QPEAGTWLNI DGFHMGVGGD 1000
    DSWSPSVSAE YQLSAGRYHY QISWR 1025
    Length:1,025
    Mass (Da):116,279
    Last modified:October 23, 2007 - v1
    Checksum:i446901FFBF2DEC14
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000822 Genomic DNA. Translation: ABV13931.1.
    RefSeqiWP_012133644.1. NC_009792.1.
    YP_001454367.1. NC_009792.1.

    Genome annotation databases

    EnsemblBacteriaiABV13931; ABV13931; CKO_02825.
    GeneIDi5581703.
    KEGGicko:CKO_02825.
    PATRICi20388327. VBICitKos71230_2363.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000822 Genomic DNA. Translation: ABV13931.1 .
    RefSeqi WP_012133644.1. NC_009792.1.
    YP_001454367.1. NC_009792.1.

    3D structure databases

    ProteinModelPortali A8AKB8.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 290338.CKO_02825.

    Protein family/group databases

    CAZyi GH2. Glycoside Hydrolase Family 2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABV13931 ; ABV13931 ; CKO_02825 .
    GeneIDi 5581703.
    KEGGi cko:CKO_02825.
    PATRICi 20388327. VBICitKos71230_2363.

    Phylogenomic databases

    eggNOGi COG3250.
    HOGENOMi HOG000252443.
    KOi K01190.
    OMAi DPKKPYI.
    OrthoDBi EOG6XWV0T.

    Enzyme and pathway databases

    BioCyci CKOS290338:GJ8L-2818-MONOMER.

    Family and domain databases

    Gene3Di 2.60.120.260. 1 hit.
    2.60.40.320. 2 hits.
    2.70.98.10. 1 hit.
    3.20.20.80. 1 hit.
    HAMAPi MF_01687. Beta_gal.
    InterProi IPR004199. B-gal_small/dom_5.
    IPR011013. Gal_mutarotase_SF_dom.
    IPR008979. Galactose-bd-like.
    IPR014718. Glyco_hydro-type_carb-bd_sub.
    IPR006101. Glyco_hydro_2.
    IPR013812. Glyco_hydro_2/20_Ig-like.
    IPR023232. Glyco_hydro_2_AS.
    IPR023933. Glyco_hydro_2_beta_Galsidase.
    IPR023230. Glyco_hydro_2_CS.
    IPR006102. Glyco_hydro_2_Ig-like.
    IPR006104. Glyco_hydro_2_N.
    IPR006103. Glyco_hydro_2_TIM.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    Pfami PF02929. Bgal_small_N. 1 hit.
    PF00703. Glyco_hydro_2. 1 hit.
    PF02836. Glyco_hydro_2_C. 1 hit.
    PF02837. Glyco_hydro_2_N. 1 hit.
    [Graphical view ]
    PRINTSi PR00132. GLHYDRLASE2.
    SMARTi SM01038. Bgal_small_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49303. SSF49303. 2 hits.
    SSF49785. SSF49785. 1 hit.
    SSF51445. SSF51445. 1 hit.
    SSF74650. SSF74650. 1 hit.
    PROSITEi PS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
    PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC BAA-895 / CDC 4225-83 / SGSC4696.

    Entry informationi

    Entry nameiBGAL_CITK8
    AccessioniPrimary (citable) accession number: A8AKB8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 24, 2009
    Last sequence update: October 23, 2007
    Last modified: October 1, 2014
    This is version 44 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3