A8AIX5 (RLMF_CITK8) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosomal RNA large subunit methyltransferase F
EC=2.1.1.181

Alternative name(s):
23S rRNA mA1618 methyltransferase
rRNA adenine N-6-methyltransferase

Gene names

Name:	rlmF
Ordered Locus Names:	CKO_02316

Organism

Citrobacter koseri (strain ATCC BAA-895 / CDC 4225-83 / SGSC4696) [Complete proteome] [HAMAP]

Taxonomic identifier

290338 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Citrobacter

Protein attributes

Sequence length	308 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically methylates the adenine in position 1618 of 23S rRNA By similarity. HAMAP MF_01848
Catalytic activity	S-adenosyl-L-methionine + adenine(1618) in 23S rRNA = S-adenosyl-L-homocysteine + rRNA containing N(6)-methyladenine(1618) in 23S rRNA. HAMAP MF_01848
Subcellular location	Cytoplasm Potential HAMAP MF_01848.
Sequence similarities	Belongs to the methyltransferase superfamily. METTL16/rlmF family.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	rRNA (adenine-N6-)-methyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 308	308	Ribosomal RNA large subunit methyltransferase F HAMAP MF_01848		PRO_0000349898

Sequences

	Sequence		Length	Mass (Da)
	A8AIX5 [UniParc]. Last modified October 23, 2007. Version 1. Checksum: E5886F591FAE0B47	FASTA	308	34,254
	10 20 30 40 50 60 MSAQKPGLHP RNRHHHRYDL ATLCQVTPEL AQFLTRTPAG EQSVDFANPL AVKALNKALL 70 80 90 100 110 120 AYFYQVANWD IPEGFLCPPV PGRADYIHHL ADLLGETTGA IPADASVLDV GTGANCIYPL 130 140 150 160 170 180 IGVHEYGWRF TGSEVHAQAL ASAQVIISGN PGLTRSIRLR RQKDPAAIFN GIIHKNEQYD 190 200 210 220 230 240 ATLCNPPFHD SAASARAGSE RKRRNLGQDK NDALNFGGQQ QELWCEGGEV AFIKTMIAES 250 260 270 280 290 300 QAFGRQVMWF TTLVSRGENL PPLYRALTDV GAVKVVKKEM AQGQKQSRFI AWTFMDDDQR RRFMARKR

References

[1]

The Citrobacter koseri Genome Sequencing Project McClelland M., Sanderson E.K., Porwollik S., Spieth J., Clifton W.S., Latreille P., Courtney L., Wang C., Pepin K., Bhonagiri V., Nash W., Johnson M., Thiruvilangam P., Wilson R. Submitted (AUG-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-895 / CDC 4225-83 / SGSC4696.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000822 Genomic DNA. Translation: ABV13438.1.
RefSeq	YP_001453874.1. NC_009792.1.
3D structure databases
ProteinModelPortal	A8AIX5.
ModBase	Search...
Protein-protein interaction databases
STRING	A8AIX5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5582988.
GenomeReviews	Gene locus CKO_02316 in contig CP000822_GR.
KEGG	cko:CKO_02316.
PATRIC	20387437. VBICitKos71230_1931.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG3129.
HOGENOM	HBG747880.
OMA	DFANPEA.
ProtClustDB	PRK11727.
Family and domain databases
HAMAP	MF_01848. 23SrRNA_methyltr_F. [Tree]
InterPro	IPR016909. rRNA_lsu_methyltransferase_F. IPR010286. SAM-Mtase_prd. [Graphical view]
KO	K06970.
PANTHER	PTHR13393. DUF890. 1 hit.
Pfam	PF05971. Methyltransf_10. 1 hit. [Graphical view]
PIRSF	PIRSF029038. Mtase_YbiN_prd. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RLMF_CITK8

Accession

Primary (citable) accession number: A8AIX5

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	October 23, 2007
Last modified:	January 25, 2012
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program