ID   GHRA_CITK8              Reviewed;         312 AA.
AC   A8AI49;
DT   02-SEP-2008, integrated into UniProtKB/Swiss-Prot.
DT   23-OCT-2007, sequence version 1.
DT   16-JUN-2009, entry version 12.
DE   RecName: Full=Glyoxylate/hydroxypyruvate reductase A;
DE            EC=1.1.1.79;
DE            EC=1.1.1.81;
DE   AltName: Full=2-ketoacid reductase;
GN   Name=ghrA; OrderedLocusNames=CKO_02036;
OS   Citrobacter koseri (strain ATCC BAA-895 / CDC 4225-83 / SGSC4696).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Citrobacter.
OX   NCBI_TaxID=290338;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   McClelland M., Sanderson E.K., Porwollik S., Spieth J., Clifton W.S.,
RA   Latreille P., Courtney L., Wang C., Pepin K., Bhonagiri V., Nash W.,
RA   Johnson M., Thiruvilangam P., Wilson R.;
RL   Submitted (AUG-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes the NADPH-dependent reduction of glyoxylate
CC       and hydroxypyruvate into glycolate and glycerate, respectively (By
CC       similarity).
CC   -!- CATALYTIC ACTIVITY: Glycolate + NADP(+) = glyoxylate + NADPH.
CC   -!- CATALYTIC ACTIVITY: D-glycerate + NAD(P)(+) = hydroxypyruvate +
CC       NAD(P)H.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm (Probable).
CC   -!- SIMILARITY: Belongs to the D-isomer specific 2-hydroxyacid
CC       dehydrogenase family. GhrA subfamily.
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DR   EMBL; CP000822; ABV13162.1; -; Genomic_DNA.
DR   RefSeq; YP_001453598.1; -.
DR   GeneID; 5582217; -.
DR   GenomeReviews; CP000822_GR; CKO_02036.
DR   KEGG; cko:CKO_02036; -.
DR   OMA; A8AI49; YALVWRA.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005886; C:plasma membrane; IEA:HAMAP.
DR   GO; GO:0048037; F:cofactor binding; IEA:InterPro.
DR   GO; GO:0030267; F:glyoxylate reductase (NADP) activity; IEA:HAMAP.
DR   GO; GO:0016618; F:hydroxypyruvate reductase activity; IEA:HAMAP.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   HAMAP; MF_01666; -; 1.
DR   InterPro; IPR006140; D-isomer_2_OHA_DH_NAD-bd.
DR   InterPro; IPR016040; NAD(P)-bd_dom.
DR   Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 1.
DR   Pfam; PF02826; 2-Hacid_dh_C; 1.
DR   PROSITE; PS00065; D_2_HYDROXYACID_DH_1; FALSE_NEG.
DR   PROSITE; PS00670; D_2_HYDROXYACID_DH_2; FALSE_NEG.
DR   PROSITE; PS00671; D_2_HYDROXYACID_DH_3; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Cytoplasm; NAD; NADP; Oxidoreductase.
FT   CHAIN         1    312       Glyoxylate/hydroxypyruvate reductase A.
FT                                /FTId=PRO_0000348353.
FT   ACT_SITE    227    227       By similarity.
FT   ACT_SITE    275    275       Proton donor (By similarity).
SQ   SEQUENCE   312 AA;  34902 MW;  2441E491141E93D6 CRC64;
     MEIMFYHPTF DTAWWLNALA KAIPGANIRE WKPGDNEPAD YALVWHPPVE MLEGRKLKAV
     FALGAGVDSI LSKLKAHPEM LDASIPLFRL EDTGMGLQMQ EYAVSQVLHW FRRFDDYQAL
     KNQSTWQPLP EYDRDEFTVG IMGAGVLGAK VAEALQAWGF PLRCWSRSRK SWPGVESFAG
     AEELGAFLNQ TRVLINLLPN TAETVGIINS GLLNQLRDGA YLLNLARGVH VNEDDLLAAL
     NSEKLKGAML DVFSREPLPK ESPLWQHPRV AMTPHIAAVT RPAEAVDYIS RTITHLERGE
     SVTGQVDRVR GY
//