Reviewed, UniProtKB/Swiss-Prot A8AI14 (PYRC_CITK8)

Names and origin

Protein names

Recommended name:
    Dihydroorotase
      Short name=DHOase
    EC=3.5.2.3

Gene names

Name:	pyrC
Ordered Locus Names:	CKO_02001

Organism

Citrobacter koseri (strain ATCC BAA-895 / CDC 4225-83 / SGSC4696) [Complete proteome] [HAMAP]

Taxonomic identifier

290338 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Citrobacter

Protein attributes

Sequence length	347 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-dihydroorotate + H2O = N-carbamoyl-L-aspartate. HAMAP MF_00219
Cofactor	Binds 2 zinc ions per subunit By similarity. HAMAP MF_00219
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 3/3. HAMAP MF_00219
Subunit structure	Homodimer By similarity. HAMAP MF_00219
Sequence similarities	Belongs to the DHOase family. Type 1 subfamily.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	pyrimidine base biosynthetic process Inferred from electronic annotation. Source: InterPro pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	dihydroorotase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 347	347	Dihydroorotase HAMAP MF_00219		PRO_1000024008
Sites
	Metal binding	17	1	Zinc 1 By similarity
	Metal binding	19	1	Zinc 1 By similarity
	Metal binding	103	1	Zinc 1; via carbamate group By similarity
	Metal binding	103	1	Zinc 2; via carbamate group By similarity
	Metal binding	140	1	Zinc 2 By similarity
	Metal binding	178	1	Zinc 2 By similarity
	Metal binding	251	1	Zinc 1 By similarity
Amino acid modifications
	Modified residue	103	1	N6-carboxylysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	A8AI14-1 [UniParc]. Last modified October 23, 2007. Version 1. Checksum: 9A1EEBF605745458	FASTA	347	38,731
	10 20 30 40 50 60 MTAPSQVLKI RRPDDWHVHL RDGDMLKTVV PYTSEIYGRA IVMPNLAPPV TTVDAAIAYR 70 80 90 100 110 120 QRILDAVPAG HDFTPLMTCY LTDTLDPDEL ERGFREGVFT AAKLYPANAT TNSSHGVTSV 130 140 150 160 170 180 DTIMPVLERM EKLGMPLLVH GEVTHADIDI FDREARFIET VMEPLRQRLT ALKVVFEHIT 190 200 210 220 230 240 TKDAAEYVRD GNELLAATIT PQHLMFNRNH MLVGGIRPHL YCLPILKRNV HQQALRELVA 250 260 270 280 290 300 SGFSRAFLGT DSAPHARHRK ETSCGCAGCF NAPTALGSYA TVFEEMNALA HFEAFCSLNG 310 320 330 340 PRFYGLSVNE TYVELVREEQ LVPESITLAD DSLVPFLGGE RVRWSVK

References

[1]	McClelland M., Sanderson E.K., Porwollik S., Spieth J., Clifton W.S., Latreille P., Courtney L., Wang C., Pepin K., Bhonagiri V., Nash W., Johnson M., Thiruvilangam P., Wilson R. Submitted (AUG-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000822 Genomic DNA. Translation: ABV13127.1.
RefSeq	YP_001453563.1.
3D structure databases
SMR	A8AI14. Positions 5-347.
ModBase	Search...
Protein-protein interaction databases
STRING	A8AI14.
Genome annotation databases
GeneID	5582931.
GenomeReviews	Gene locus CKO_02001 in contig CP000822_GR.
KEGG	cko:CKO_02001.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0418.
HOGENOM	HBG628648.
OMA	CLPVAKR.
Family and domain databases
HAMAP	MF_00219. PyrC_type1. [Tree]
InterPro	IPR006680. Amidohydro_1. IPR004721. DHOdimr. IPR002195. Dihydroorotase_CS. [Graphical view]
Pfam	PF01979. Amidohydro_1. 1 hit. [Graphical view]
PIRSF	PIRSF001237. DHOdimr. 1 hit.
TIGRFAMs	TIGR00856. pyrC_dimer. 1 hit.
PROSITE	PS00482. DIHYDROOROTASE_1. 1 hit. PS00483. DIHYDROOROTASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRC_CITK8

Accession

Primary (citable) accession number: A8AI14

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 23, 2007
Last modified:	February 9, 2010
This is version 19 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)