A8AHD6 (ASTE_CITK8) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Succinylglutamate desuccinylase
EC=3.5.1.96

Gene names

Name:	astE
Ordered Locus Names:	CKO_01770

Organism

Citrobacter koseri (strain ATCC BAA-895 / CDC 4225-83 / SGSC4696) [Complete proteome] [HAMAP]

Taxonomic identifier

290338 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Citrobacter

Protein attributes

Sequence length	322 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Transforms N(2)-succinylglutamate into succinate and glutamate By similarity. HAMAP MF_00767
Catalytic activity	N-succinyl-L-glutamate + H2O = succinate + L-glutamate. HAMAP MF_00767
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00767
Pathway	Amino-acid degradation; L-arginine degradation via AST pathway; L-glutamate and succinate from L-arginine: step 5/5. HAMAP MF_00767
Sequence similarities	Belongs to the AspA/AstE family. Succinylglutamate desuccinylase subfamily.

Ontologies

Keywords
Biological process	Arginine metabolism
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine catabolic process to glutamate Inferred from electronic annotation. Source: InterPro
Molecular function	hydrolase activity, acting on ester bonds Inferred from electronic annotation. Source: InterPro succinylglutamate desuccinylase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 322	322	Succinylglutamate desuccinylase HAMAP MF_00767		PRO_1000017321
Sites
	Active site	210	1	Potential
	Metal binding	53	1	Zinc By similarity
	Metal binding	56	1	Zinc By similarity
	Metal binding	147	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	A8AHD6 [UniParc]. Last modified October 23, 2007. Version 1. Checksum: 374C8CB722F14E35	FASTA	322	36,046
	10 20 30 40 50 60 MDNFLAQTLA GISPDITQGE TESFHWRWQM PGVLELTPAV EVDRALVLSA GIHGNETAPV 70 80 90 100 110 120 EMLAALLSAL FEGKIPLRWR LLVILGNPPA LAEGVRYCHS DMNRMFGGRW QMFEESGETL 130 140 150 160 170 180 RARELEQLLE DFYSRRKTRI RWHLDLHTAI RGSRHVRFGV LPQRSIPWDE HFLSWLGAAG 190 200 210 220 230 240 LEALVFHQTP GGTFTHFSCE HFGALSCTLE LGKALPFGHN DLTQFTPTAQ ALATLLAGEK 250 260 270 280 290 300 PMEGGTRPIR YRVVAQITRR SDAFVLCMDN QTLNFTPFKK GTLLAQDGDE QVIVTHDVEY 310 320 VLFPNPNVAC GLRAGLMLEK LA

References

[1]

The Citrobacter koseri Genome Sequencing Project McClelland M., Sanderson E.K., Porwollik S., Spieth J., Clifton W.S., Latreille P., Courtney L., Wang C., Pepin K., Bhonagiri V., Nash W., Johnson M., Thiruvilangam P., Wilson R. Submitted (AUG-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-895 / CDC 4225-83 / SGSC4696.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000822 Genomic DNA. Translation: ABV12899.1.
RefSeq	YP_001453335.1. NC_009792.1.
3D structure databases
ProteinModelPortal	A8AHD6.
SMR	A8AHD6. Positions 1-322.
ModBase	Search...
Protein-protein interaction databases
STRING	A8AHD6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5583622.
GenomeReviews	Gene locus CKO_01770 in contig CP000822_GR.
KEGG	cko:CKO_01770.
PATRIC	20386549. VBICitKos71230_1490.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2988.
HOGENOM	HBG298271.
OMA	RWRWLGH.
ProtClustDB	PRK05324.
Family and domain databases
HAMAP	MF_00767. Arg_catab_AstE. [Tree]
InterPro	IPR007036. Aste_AspA. IPR016681. SuccinylGlu_desuccinylase. [Graphical view]
KO	K05526.
Pfam	PF04952. AstE_AspA. 1 hit. [Graphical view]
PIRSF	PIRSF017020. AstE. 1 hit.
TIGRFAMs	TIGR03242. Arg_catab_astE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ASTE_CITK8

Accession

Primary (citable) accession number: A8AHD6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 23, 2007
Last modified:	January 25, 2012
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program