Reviewed, UniProtKB/Swiss-Prot A8AGN6 (MAO1_CITK8)

Names and origin

Protein names

Recommended name:
    NAD-dependent malic enzyme
      Short name=NAD-ME
    EC=1.1.1.38

Gene names

Name:	sfcA
Ordered Locus Names:	CKO_01517

Organism

Citrobacter koseri (strain ATCC BAA-895 / CDC 4225-83 / SGSC4696) [Complete proteome] [HAMAP]

Taxonomic identifier

290338 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Citrobacter

Protein attributes

Sequence length	565 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-malate + NAD(+) = pyruvate + CO(2) + NADH.
Cofactor	Divalent metal cations. Prefers magnesium or manganese By similarity.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the malic enzymes family.

Ontologies

Keywords
Ligand	Metal-binding NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	malate metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NAD binding Inferred from electronic annotation. Source: InterPro malate dehydrogenase (oxaloacetate-decarboxylating) activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 565	565	NAD-dependent malic enzyme		PRO_1000069527
Sites
	Active site	104	1	Proton donor By similarity
	Active site	175	1	Proton acceptor By similarity
	Metal binding	246	1	Divalent metal cation By similarity
	Metal binding	247	1	Divalent metal cation By similarity
	Metal binding	270	1	Divalent metal cation By similarity
	Binding site	157	1	NAD By similarity
	Binding site	270	1	NAD By similarity
	Binding site	418	1	NAD By similarity
	Site	270	1	Important for activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	A8AGN6-1 [UniParc]. Last modified October 23, 2007. Version 1. Checksum: B516B2A6989E620F	FASTA	565	63,172
	10 20 30 40 50 60 METKIKKQRS LYIPYAGPVL LEFPLLNKGS AFSIEERRNF NLLGLLPEVV ESIEEQAERA 70 80 90 100 110 120 WIQYQGFKTE IDKHIYLRNI QDTNETLFYR LVQNHLDEMM PVIYTPTVGA ACERFSEIYR 130 140 150 160 170 180 RARGVFISYQ NRHNMDDILQ NVPNHNIKVI VVTDGERILG LGDQGIGGMG IPIGKLSLYT 190 200 210 220 230 240 ACGGISPAYT LPVVLDVGTN NQQLLNDPLY MGWRNPRITD DEYYEFVDEF IQAVKQRWPD 250 260 270 280 290 300 VLLQFEDFAQ KNAMPLLNRY RDEICSFNDD IQGTAAVTVG TLIAASRAAG SQLSEQKIVF 310 320 330 340 350 360 LGAGSAGCGI AEQIIAQTQR EGLSEEAARQ KVFMVDRFGL LTDQMPNLLS FQTKLVQKRE 370 380 390 400 410 420 NLQHWDSESE VLSLLDVVRN VKPDILIGVS GQTGLFTEEI IREMHKHCPR PIVMPLSNPT 430 440 450 460 470 480 SRVEATPQDI IAWTEGNALV ATGSPFAPVA WKDKIYPIAQ CNNAYIFPGI GLGVIASGAS 490 500 510 520 530 540 RITDEMLMSA SETLAKYSPL VLNGEGLVLP ELKDIQTVSR AIAFAVGKMA QQQGVAVKTS 550 560 AEALQQAIDE NFWQAEYRDY RRTSI

References

[1]	McClelland M., Sanderson E.K., Porwollik S., Spieth J., Clifton W.S., Latreille P., Courtney L., Wang C., Pepin K., Bhonagiri V., Nash W., Johnson M., Thiruvilangam P., Wilson R. Submitted (AUG-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000822 Genomic DNA. Translation: ABV12649.1.
RefSeq	YP_001453085.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5583553.
GenomeReviews	Gene locus CKO_01517 in contig CP000822_GR.
KEGG	cko:CKO_01517.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_01619. [Tree]
InterPro	IPR015884. Malic_enzyme_CS. IPR012301. Malic_N. IPR012302. Malic_NAD_bd. IPR001891. Malic_OxRdtase. IPR016040. NAD(P)-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF00390. malic. 1 hit. PF03949. Malic_M. 1 hit. [Graphical view]
PRINTS	PR00072. MALOXRDTASE.
PROSITE	PS00331. MALIC_ENZYMES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MAO1_CITK8

Accession

Primary (citable) accession number: A8AGN6

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	October 23, 2007
Last modified:	November 25, 2008
This is version 12 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)