A8AFT6 (TREA_CITK8) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Periplasmic trehalase
EC=3.2.1.28

Alternative name(s):
Alpha,alpha-trehalase
Alpha,alpha-trehalose glucohydrolase

Gene names

Name:	treA
Ordered Locus Names:	CKO_01209

Organism

Citrobacter koseri (strain ATCC BAA-895 / CDC 4225-83 / SGSC4696) [Complete proteome] [HAMAP]

Taxonomic identifier

290338 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Citrobacter

Protein attributes

Sequence length	570 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Provides the cells with the ability to utilize trehalose at high osmolarity by splitting it into glucose molecules that can subsequently be taken up by the phosphotransferase-mediated uptake system By similarity. HAMAP MF_01060
Catalytic activity	Alpha,alpha-trehalose + H2O = 2 D-glucose. HAMAP MF_01060
Subunit structure	Monomer By similarity. HAMAP MF_01060
Subcellular location	Periplasm By similarity HAMAP MF_01060.
Sequence similarities	Belongs to the glycosyl hydrolase 37 family.

Ontologies

Keywords
Cellular component	Periplasm
Domain	Signal
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cellular hyperosmotic response Inferred from electronic annotation. Source: InterPro trehalose catabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	periplasmic space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	alpha,alpha-trehalase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 34	34	Potential
	Chain	35 – 570	536	Periplasmic trehalase HAMAP MF_01060		PRO_1000064449

Sequences

	Sequence		Length	Mass (Da)
	A8AFT6 [UniParc]. Last modified October 23, 2007. Version 1. Checksum: A8495519A827F8D3	FASTA	570	63,731
	10 20 30 40 50 60 MITPALRHSG TLSFAIKLTV ASTLLTFASL SAHAEEQPAS PPQPPDILLG PLFNDVQSVK 70 80 90 100 110 120 LFPDQKTFAD AVPNSDPLMI LADYRMQRNQ SGFDLRHFVD VNFTLPKEGE KYVPPEGQSL 130 140 150 160 170 180 REHIDGLWPV LTRTTESAGK WDSLLPLPEP YVVPGGRFRE VYYWDSYFTM LGLAESDHWD 190 200 210 220 230 240 KVADMVANFG YELDSWGHIP NGNRTYYLSR SQPPFFAFMV ELLAQHEGDD ALKKYLPQLQ 250 260 270 280 290 300 KEYAYWMEGV ENLQPGEQNK RVVKLDDGTV LNRYWDDRDT PRPESWMEDI TTAKSNPNRP 310 320 330 340 350 360 ATEIYRDLRS AAASGWDFSS RWMDDPNQLS TIRTTSIVPV DLNALLYKLE KMLARASKAA 370 380 390 400 410 420 GDDANANQYE ALASARQKGI ETHLWNNQEG WYADYDLKSK KVRNQLTAAT LFPLYVNAAA 430 440 450 460 470 480 KDRASKVAAA TQAHLLQPGG LSTTSVKSGQ QWDAPNGWAP LQWVATEGLQ NYGQDNVAMD 490 500 510 520 530 540 VTWRFLTNVQ HTYDREQKLV EKYDVSSTGT GGGGGEYPLQ DGFGWTNGVT LKMLDLICPK 550 560 570 EKPCDSVPAT RPAAPGASQP APQKQVETTP

References

[1]

The Citrobacter koseri Genome Sequencing Project McClelland M., Sanderson E.K., Porwollik S., Spieth J., Clifton W.S., Latreille P., Courtney L., Wang C., Pepin K., Bhonagiri V., Nash W., Johnson M., Thiruvilangam P., Wilson R. Submitted (AUG-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-895 / CDC 4225-83 / SGSC4696.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000822 Genomic DNA. Translation: ABV12349.1.
RefSeq	YP_001452785.1. NC_009792.1.
3D structure databases
ProteinModelPortal	A8AFT6.
SMR	A8AFT6. Positions 42-552.
ModBase	Search...
Protein-protein interaction databases
STRING	A8AFT6.
Protein family/group databases
CAZy	GH37. Glycoside Hydrolase Family 37.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5584960.
GenomeReviews	Gene locus CKO_01209 in contig CP000822_GR.
KEGG	cko:CKO_01209.
PATRIC	20385603. VBICitKos71230_1023.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1626.
HOGENOM	HBG485982.
OMA	NRYWDAS.
ProtClustDB	PRK13271.
Family and domain databases
HAMAP	MF_01060. Peripl_trehalase. [Tree]
InterPro	IPR008928. 6-hairpin_glycosidase-like. IPR001661. Glyco_hydro_37. IPR018232. Glyco_hydro_37_CS. IPR023720. Trehalase_periplasmic. [Graphical view]
KO	K01194.
PANTHER	PTHR23403. Glyco_hydro_37. 1 hit.
Pfam	PF01204. Trehalase. 1 hit. [Graphical view]
PRINTS	PR00744. GLHYDRLASE37.
SUPFAM	SSF48208. Glyco_trans_6hp. 1 hit.
PROSITE	PS00927. TREHALASE_1. 1 hit. PS00928. TREHALASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TREA_CITK8

Accession

Primary (citable) accession number: A8AFT6

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	October 23, 2007
Last modified:	January 25, 2012
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program