A8AFS0 (DADA_CITK8) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
D-amino acid dehydrogenase small subunit
EC=1.4.99.1

Gene names

Name:	dadA
Ordered Locus Names:	CKO_01193

Organism

Citrobacter koseri (strain ATCC BAA-895 / CDC 4225-83 / SGSC4696) [Complete proteome] [HAMAP]

Taxonomic identifier

290338 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Citrobacter

Protein attributes

Sequence length	432 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Oxidative deamination of D-amino acids By similarity. HAMAP MF_01202
Catalytic activity	A D-amino acid + H2O + acceptor = a 2-oxo acid + NH3 + reduced acceptor. HAMAP MF_01202
Cofactor	FAD By similarity. HAMAP MF_01202
Pathway	Amino-acid degradation; D-alanine degradation; NH(3) and pyruvate from D-alanine: step 1/1. HAMAP MF_01202
Subunit structure	Heterodimer of a small and a large subunit By similarity.
Sequence similarities	Belongs to the DadA oxidoreductase family.

Ontologies

Keywords
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	D-amino-acid dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 432	432	D-amino acid dehydrogenase small subunit HAMAP MF_01202		PRO_1000066088
Regions
	Nucleotide binding	3 – 17	15	FAD Potential

Sequences

	Sequence		Length	Mass (Da)
	A8AFS0 [UniParc]. Last modified October 23, 2007. Version 1. Checksum: 08083788AF42A7B1	FASTA	432	47,691
	10 20 30 40 50 60 MRVVILGSGV VGVTSAWYLC QAGHDVTVID REPGPALETS AANAGQISPG YAAPWAAPGV 70 80 90 100 110 120 PLKAIKWMFQ RHAPLAVRLD GTQFQLKWMW QMLRNCDTRH YMENKGRMVR LAEYSRDCLK 130 140 150 160 170 180 ALRASTGIQY EGRQGGTLQL FRTAQQYENA TRDIAVLEDA GVPYQLLESS RLAEVEPALA 190 200 210 220 230 240 EVAHKLTGGL RLPNDETGDC QLFTQRLAQM AEQAGVTFRF NTPVEKLLYE GEQIYGVKCG 250 260 270 280 290 300 DDIIKADAYV MAFGSYSTAM LKGIVDIPVY PLKGYSLTIP VAQDDGAPVS TILDETYKIA 310 320 330 340 350 360 ITRFDKRIRV GGMAEIVGFN TELLQPRRET LEMVVRDLFP RGGHVEQATF WTGLRPMTPD 370 380 390 400 410 420 GTPVIGRTRF KNLWLNTGHG TLGWTMACGS GQLLSDILSG RTPAIPYDDL SVARYSPGFT 430 PSRPRHLHGA HN

References

[1]

The Citrobacter koseri Genome Sequencing Project McClelland M., Sanderson E.K., Porwollik S., Spieth J., Clifton W.S., Latreille P., Courtney L., Wang C., Pepin K., Bhonagiri V., Nash W., Johnson M., Thiruvilangam P., Wilson R. Submitted (AUG-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-895 / CDC 4225-83 / SGSC4696.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000822 Genomic DNA. Translation: ABV12333.1.
RefSeq	YP_001452769.1. NC_009792.1.
3D structure databases
ProteinModelPortal	A8AFS0.
ModBase	Search...
Protein-protein interaction databases
STRING	A8AFS0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5583101.
GenomeReviews	Gene locus CKO_01193 in contig CP000822_GR.
KEGG	cko:CKO_01193.
PATRIC	20385575. VBICitKos71230_1009.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0665.
HOGENOM	HBG729204.
OMA	VDRQPAV.
ProtClustDB	PRK00711.
Family and domain databases
HAMAP	MF_01202. DadA. [Tree]
InterPro	IPR023080. D-aa_DH_ssu_DadA. IPR006076. FAD-dep_OxRdtase. [Graphical view]
KO	K00285.
Pfam	PF01266. DAO. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DADA_CITK8

Accession

Primary (citable) accession number: A8AFS0

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	October 23, 2007
Last modified:	January 25, 2012
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program