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Protein

Ribosomal RNA small subunit methyltransferase F

Gene

rsmF

Organism
Citrobacter koseri (strain ATCC BAA-895 / CDC 4225-83 / SGSC4696)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Specifically methylates the cytosine at position 1407 (m5C1407) of 16S rRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + cytosine(1407) in 16S rRNA = S-adenosyl-L-homocysteine + 5-methylcytosine(1407) in 16S rRNA.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei149S-adenosyl-L-methionineUniRule annotation1
Binding sitei176S-adenosyl-L-methionineUniRule annotation1
Binding sitei194S-adenosyl-L-methionineUniRule annotation1
Active sitei247NucleophileUniRule annotation1

GO - Molecular functioni

Keywordsi

Molecular functionMethyltransferase, RNA-binding, Transferase
Biological processrRNA processing
LigandS-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosomal RNA small subunit methyltransferase FUniRule annotation (EC:2.1.1.178UniRule annotation)
Alternative name(s):
16S rRNA m5C1407 methyltransferaseUniRule annotation
rRNA (cytosine-C(5)-)-methyltransferase RsmFUniRule annotation
Gene namesi
Name:rsmFUniRule annotation
Ordered Locus Names:CKO_01138
OrganismiCitrobacter koseri (strain ATCC BAA-895 / CDC 4225-83 / SGSC4696)
Taxonomic identifieri290338 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeCitrobacter
Proteomesi
  • UP000008148 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000692711 – 479Ribosomal RNA small subunit methyltransferase FAdd BLAST479

Interactioni

Protein-protein interaction databases

STRINGi290338.CKO_01138.

Structurei

3D structure databases

ProteinModelPortaliA8AFL6.
SMRiA8AFL6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni125 – 131S-adenosyl-L-methionine bindingUniRule annotation7

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CYJ. Bacteria.
COG0144. LUCA.
COG3270. LUCA.
HOGENOMiHOG000218115.
KOiK11392.
OMAiDPLFHAG.
OrthoDBiPOG091H02E2.

Family and domain databases

HAMAPiMF_01579. 16SrRNA_methyltr_F. 1 hit.
InterProiView protein in InterPro
IPR018314. Fmu/NOL1/Nop2p_CS.
IPR031341. Methyltr_RsmF_N.
IPR001678. MeTrfase_RsmB/NOP2.
IPR027391. Nol1_Nop2_Fmu_2.
IPR011023. Nop2p.
IPR023267. RCMT.
IPR023545. rRNA_ssu_MeTfrase_F.
IPR029063. SAM-dependent_MTases.
PfamiView protein in Pfam
PF01189. Methyltr_RsmB-F. 1 hit.
PF17125. Methyltr_RsmF_N. 1 hit.
PF13636. Methyltranf_PUA. 1 hit.
PRINTSiPR02008. RCMTFAMILY.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00446. nop2p. 1 hit.
PROSITEiView protein in PROSITE
PS01153. NOL1_NOP2_SUN. 1 hit.
PS51686. SAM_MT_RSMB_NOP. 1 hit.

Sequencei

Sequence statusi: Complete.

A8AFL6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQHAVYFPD AFLTQMREAM PSTLSFDDFL AACQRPLRRS IRVNTLKISV
60 70 80 90 100
TDFLALTAPY GWSLTPVPWC EEGFWIERDD EESLPLGSTA EHLSGLFYIQ
110 120 130 140 150
EASSMLPVAA LFAEGNTPER IMDVAAAPGS KTTQIAARMN NQGAILANEF
160 170 180 190 200
SASRVKVLHA NISRCGISNV ALTHFDGRVF GAALPETFDA ILLDAPCSGE
210 220 230 240 250
GVVRKDPDAL KNWSVESNLD IAATQRELID SAFHALRPGG TLVYSTCTLN
260 270 280 290 300
REENESVCLW LKETYPEAVE FLPLGDLFPG AERALTAEGF LHVFPQIYDC
310 320 330 340 350
EGFFVARLRK TAAIPPLPAP GYKVGKFPFA PMKGREATQI TQAANASGLQ
360 370 380 390 400
WGENLHLWQR DKEVWLFPAE IEALIGKVRF SRLGIKLAES HNKGYRWQHE
410 420 430 440 450
AVIALADPHH ANAFELSHQE AEEWYRGRDV YPQTAPSADD VLVTFQHQPI
460 470
GLAKRIGSRL KNSYPRELVR DGKLFTGND
Length:479
Mass (Da):53,143
Last modified:October 23, 2007 - v1
Checksum:iE7385846FD355F72
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000822 Genomic DNA. Translation: ABV12279.1.
RefSeqiWP_012132033.1. NC_009792.1.

Genome annotation databases

EnsemblBacteriaiABV12279; ABV12279; CKO_01138.
KEGGicko:CKO_01138.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiRSMF_CITK8
AccessioniPrimary (citable) accession number: A8AFL6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: October 23, 2007
Last modified: June 7, 2017
This is version 67 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families