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A8AFD4 (OTSA_CITK8) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 35. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Alpha,alpha-trehalose-phosphate synthase [UDP-forming]

EC=2.4.1.15
Alternative name(s):
Osmoregulatory trehalose synthesis protein A
Trehalose-6-phosphate synthase
UDP-glucose-glucosephosphate glucosyltransferase
Gene names
Name:otsA
Ordered Locus Names:CKO_01054
OrganismCitrobacter koseri (strain ATCC BAA-895 / CDC 4225-83 / SGSC4696) [Complete proteome] [HAMAP]
Taxonomic identifier290338 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeCitrobacter

Protein attributes

Sequence length473 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the transfer of glucose from UDP-glucose to glucose-6-phosphate to form alpha,alpha-1,1 trehalose-6-phosphate. Acts with retention of the anomeric configuration of the UDP-sugar donor By similarity.

Catalytic activity

UDP-glucose + D-glucose 6-phosphate = UDP + alpha,alpha-trehalose 6-phosphate.

Pathway

Glycan biosynthesis; trehalose biosynthesis.

Subunit structure

Homotetramer By similarity.

Sequence similarities

Belongs to the glycosyltransferase 20 family.

Sequence caution

The sequence ABV12197.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Molecular functionGlycosyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processtrehalose biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Molecular_functionalpha,alpha-trehalose-phosphate synthase (UDP-forming) activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 473473Alpha,alpha-trehalose-phosphate synthase [UDP-forming]
PRO_0000348890

Regions

Region21 – 222UDP-glucose binding By similarity
Region365 – 3695UDP-glucose binding By similarity

Sites

Binding site101Glucose-6-phosphate By similarity
Binding site761Glucose-6-phosphate By similarity
Binding site1301Glucose-6-phosphate By similarity
Binding site2621UDP-glucose By similarity
Binding site2671UDP-glucose By similarity
Binding site3001Glucose-6-phosphate By similarity
Binding site3391UDP-glucose; via amide nitrogen and carbonyl oxygen By similarity
Site851Involved in alpha anomer selectivity By similarity
Site1551Involved in alpha anomer selectivity By similarity

Sequences

Sequence LengthMass (Da)Tools
A8AFD4 [UniParc].

Last modified September 2, 2008. Version 2.
Checksum: 0A46A76686A24EC6

FASTA47353,476
        10         20         30         40         50         60 
MGRLVVVSNR IAPPDNKGGA GGLAVGVLGA LKAAGGLWFG WSGETGNEDK PLKKVTRGNM 

        70         80         90        100        110        120 
TWASFNLSEQ DYEEYYCQFS NAVLWPAFHY RLDLVQFQRP AWEGYSRVNA LLADKLLPLL 

       130        140        150        160        170        180 
KDDDIIWVHD YHLLPFASEL RKRGVNNRIG FFLHIPFPTP EIFNALPPHD TLLEQLCDFD 

       190        200        210        220        230        240 
LLGFQTENDR LAFLDSLSSQ TRVTTRGSKS HSAWGKSFRT EVYPIGIEPD EIAQQASGPL 

       250        260        270        280        290        300 
PPKLAQLKAE LKNVQNIFSV ERLDYSKGLP ERFQAYEALL EKYPQHHGKI RYTQIAPTSR 

       310        320        330        340        350        360 
GEVQAYQDIR HQLETEAGRI NGKYGQLGWT PLYYLNQHFD RKLLMKVFRY SDVGLVTPLR 

       370        380        390        400        410        420 
DGMNLVAKEF VAAQDPANPG VLVLSQFAGA ANELTSALIV NPYDRDDVAV ALHRALTMPL 

       430        440        450        460        470 
AERISRHAEM LETIIKNDIN HWQQRFIRDL KDVAPRSAET LHRNKVATFP KLA 

« Hide

References

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000822 Genomic DNA. Translation: ABV12197.1. Different initiation.
RefSeqYP_001452633.1. NC_009792.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING290338.CKO_01054.

Protein family/group databases

CAZyGT20. Glycosyltransferase Family 20.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABV12197; ABV12197; CKO_01054.
GeneID5581106.
KEGGcko:CKO_01054.
PATRIC20385347. VBICitKos71230_0895.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0380.
HOGENOMHOG000191478.
KOK00697.
OrthoDBEOG6CCH1M.

Enzyme and pathway databases

BioCycCKOS290338:GJ8L-1051-MONOMER.
UniPathwayUPA00299.

Family and domain databases

InterProIPR001830. Glyco_trans_20.
IPR012766. Trehalose_OtsA.
[Graphical view]
PfamPF00982. Glyco_transf_20. 1 hit.
[Graphical view]
TIGRFAMsTIGR02400. trehalose_OtsA. 1 hit.
ProtoNetSearch...

Entry information

Entry nameOTSA_CITK8
AccessionPrimary (citable) accession number: A8AFD4
Entry history
Integrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: September 2, 2008
Last modified: May 14, 2014
This is version 35 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways