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Protein

Trehalose-6-phosphate synthase

Gene

otsA

Organism
Citrobacter koseri (strain ATCC BAA-895 / CDC 4225-83 / SGSC4696)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Probably involved in the osmoprotection via the biosynthesis of trehalose. Catalyzes the transfer of glucose from UDP-glucose (UDP-Glc) to D-glucose 6-phosphate (Glc-6-P) to form trehalose-6-phosphate. Acts with retention of the anomeric configuration of the UDP-sugar donor.By similarity

Catalytic activityi

UDP-glucose + D-glucose 6-phosphate = UDP + alpha,alpha-trehalose 6-phosphate.By similarity

Pathwayi: trehalose biosynthesis

This protein is involved in the pathway trehalose biosynthesis, which is part of Glycan biosynthesis.By similarity
View all proteins of this organism that are known to be involved in the pathway trehalose biosynthesis and in Glycan biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei10D-glucose 6-phosphateBy similarity1
Binding sitei76D-glucose 6-phosphateBy similarity1
Sitei85Involved in alpha anomer selectivityBy similarity1
Binding sitei130D-glucose 6-phosphateBy similarity1
Sitei155Involved in alpha anomer selectivityBy similarity1
Binding sitei262UDP-glucoseBy similarity1
Binding sitei267UDP-glucoseBy similarity1
Binding sitei300D-glucose 6-phosphateBy similarity1
Binding sitei339UDP-glucose; via amide nitrogen and carbonyl oxygenBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Transferase

Enzyme and pathway databases

UniPathwayiUPA00299.

Protein family/group databases

CAZyiGT20. Glycosyltransferase Family 20.

Names & Taxonomyi

Protein namesi
Recommended name:
Trehalose-6-phosphate synthaseBy similarity (EC:2.4.1.15By similarity)
Short name:
TPSBy similarity
Alternative name(s):
Alpha,alpha-trehalose-phosphate synthase [UDP-forming]By similarity
Osmoregulatory trehalose synthesis protein ABy similarity
Short name:
OtsABy similarity
UDP-glucose-glucosephosphate glucosyltransferaseBy similarity
Gene namesi
Name:otsABy similarity
Ordered Locus Names:CKO_01054
OrganismiCitrobacter koseri (strain ATCC BAA-895 / CDC 4225-83 / SGSC4696)
Taxonomic identifieri290338 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeCitrobacter
Proteomesi
  • UP000008148 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003488901 – 473Trehalose-6-phosphate synthaseAdd BLAST473

Proteomic databases

PRIDEiA8AFD4.

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi290338.CKO_01054.

Structurei

3D structure databases

SMRiA8AFD4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni21 – 22UDP-glucose bindingBy similarity2
Regioni365 – 369UDP-glucose bindingBy similarity5

Sequence similaritiesi

Belongs to the glycosyltransferase 20 family.By similarity

Phylogenomic databases

eggNOGiENOG4105C1K. Bacteria.
COG0380. LUCA.
HOGENOMiHOG000191478.
KOiK00697.
OrthoDBiPOG091H0ASR.

Family and domain databases

CDDicd03788. GT1_TPS. 1 hit.
InterProiView protein in InterPro
IPR001830. Glyco_trans_20.
IPR012766. Trehalose_OtsA.
PfamiView protein in Pfam
PF00982. Glyco_transf_20. 1 hit.
TIGRFAMsiTIGR02400. trehalose_OtsA. 1 hit.

Sequencei

Sequence statusi: Complete.

A8AFD4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGRLVVVSNR IAPPDNKGGA GGLAVGVLGA LKAAGGLWFG WSGETGNEDK
60 70 80 90 100
PLKKVTRGNM TWASFNLSEQ DYEEYYCQFS NAVLWPAFHY RLDLVQFQRP
110 120 130 140 150
AWEGYSRVNA LLADKLLPLL KDDDIIWVHD YHLLPFASEL RKRGVNNRIG
160 170 180 190 200
FFLHIPFPTP EIFNALPPHD TLLEQLCDFD LLGFQTENDR LAFLDSLSSQ
210 220 230 240 250
TRVTTRGSKS HSAWGKSFRT EVYPIGIEPD EIAQQASGPL PPKLAQLKAE
260 270 280 290 300
LKNVQNIFSV ERLDYSKGLP ERFQAYEALL EKYPQHHGKI RYTQIAPTSR
310 320 330 340 350
GEVQAYQDIR HQLETEAGRI NGKYGQLGWT PLYYLNQHFD RKLLMKVFRY
360 370 380 390 400
SDVGLVTPLR DGMNLVAKEF VAAQDPANPG VLVLSQFAGA ANELTSALIV
410 420 430 440 450
NPYDRDDVAV ALHRALTMPL AERISRHAEM LETIIKNDIN HWQQRFIRDL
460 470
KDVAPRSAET LHRNKVATFP KLA
Length:473
Mass (Da):53,476
Last modified:September 2, 2008 - v2
Checksum:i0A46A76686A24EC6
GO

Sequence cautioni

The sequence ABV12197 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000822 Genomic DNA. Translation: ABV12197.1. Different initiation.
RefSeqiWP_024130244.1. NC_009792.1.

Genome annotation databases

EnsemblBacteriaiABV12197; ABV12197; CKO_01054.
KEGGicko:CKO_01054.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiOTSA_CITK8
AccessioniPrimary (citable) accession number: A8AFD4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: September 2, 2008
Last modified: June 7, 2017
This is version 50 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families