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A8AFD4

- OTSA_CITK8

UniProt

A8AFD4 - OTSA_CITK8

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Protein

Alpha,alpha-trehalose-phosphate synthase [UDP-forming]

Gene

otsA

Organism
Citrobacter koseri (strain ATCC BAA-895 / CDC 4225-83 / SGSC4696)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the transfer of glucose from UDP-glucose to glucose-6-phosphate to form alpha,alpha-1,1 trehalose-6-phosphate. Acts with retention of the anomeric configuration of the UDP-sugar donor (By similarity).By similarity

Catalytic activityi

UDP-glucose + D-glucose 6-phosphate = UDP + alpha,alpha-trehalose 6-phosphate.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei10 – 101Glucose-6-phosphateBy similarity
Binding sitei76 – 761Glucose-6-phosphateBy similarity
Sitei85 – 851Involved in alpha anomer selectivityBy similarity
Binding sitei130 – 1301Glucose-6-phosphateBy similarity
Sitei155 – 1551Involved in alpha anomer selectivityBy similarity
Binding sitei262 – 2621UDP-glucoseBy similarity
Binding sitei267 – 2671UDP-glucoseBy similarity
Binding sitei300 – 3001Glucose-6-phosphateBy similarity
Binding sitei339 – 3391UDP-glucose; via amide nitrogen and carbonyl oxygenBy similarity

GO - Molecular functioni

  1. alpha,alpha-trehalose-phosphate synthase (UDP-forming) activity Source: UniProtKB-EC

GO - Biological processi

  1. trehalose biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

BioCyciCKOS290338:GJ8L-1051-MONOMER.
UniPathwayiUPA00299.

Protein family/group databases

CAZyiGT20. Glycosyltransferase Family 20.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha,alpha-trehalose-phosphate synthase [UDP-forming] (EC:2.4.1.15)
Alternative name(s):
Osmoregulatory trehalose synthesis protein A
Trehalose-6-phosphate synthase
UDP-glucose-glucosephosphate glucosyltransferase
Gene namesi
Name:otsA
Ordered Locus Names:CKO_01054
OrganismiCitrobacter koseri (strain ATCC BAA-895 / CDC 4225-83 / SGSC4696)
Taxonomic identifieri290338 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeCitrobacter
ProteomesiUP000008148: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 473473Alpha,alpha-trehalose-phosphate synthase [UDP-forming]PRO_0000348890Add
BLAST

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi290338.CKO_01054.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni21 – 222UDP-glucose bindingBy similarity
Regioni365 – 3695UDP-glucose bindingBy similarity

Sequence similaritiesi

Belongs to the glycosyltransferase 20 family.Curated

Phylogenomic databases

eggNOGiCOG0380.
HOGENOMiHOG000191478.
KOiK00697.
OrthoDBiEOG6CCH1M.

Family and domain databases

InterProiIPR001830. Glyco_trans_20.
IPR012766. Trehalose_OtsA.
[Graphical view]
PfamiPF00982. Glyco_transf_20. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02400. trehalose_OtsA. 1 hit.

Sequencei

Sequence statusi: Complete.

A8AFD4-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGRLVVVSNR IAPPDNKGGA GGLAVGVLGA LKAAGGLWFG WSGETGNEDK
60 70 80 90 100
PLKKVTRGNM TWASFNLSEQ DYEEYYCQFS NAVLWPAFHY RLDLVQFQRP
110 120 130 140 150
AWEGYSRVNA LLADKLLPLL KDDDIIWVHD YHLLPFASEL RKRGVNNRIG
160 170 180 190 200
FFLHIPFPTP EIFNALPPHD TLLEQLCDFD LLGFQTENDR LAFLDSLSSQ
210 220 230 240 250
TRVTTRGSKS HSAWGKSFRT EVYPIGIEPD EIAQQASGPL PPKLAQLKAE
260 270 280 290 300
LKNVQNIFSV ERLDYSKGLP ERFQAYEALL EKYPQHHGKI RYTQIAPTSR
310 320 330 340 350
GEVQAYQDIR HQLETEAGRI NGKYGQLGWT PLYYLNQHFD RKLLMKVFRY
360 370 380 390 400
SDVGLVTPLR DGMNLVAKEF VAAQDPANPG VLVLSQFAGA ANELTSALIV
410 420 430 440 450
NPYDRDDVAV ALHRALTMPL AERISRHAEM LETIIKNDIN HWQQRFIRDL
460 470
KDVAPRSAET LHRNKVATFP KLA
Length:473
Mass (Da):53,476
Last modified:September 2, 2008 - v2
Checksum:i0A46A76686A24EC6
GO

Sequence cautioni

The sequence ABV12197.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000822 Genomic DNA. Translation: ABV12197.1. Different initiation.
RefSeqiYP_001452633.1. NC_009792.1.

Genome annotation databases

EnsemblBacteriaiABV12197; ABV12197; CKO_01054.
GeneIDi5581106.
KEGGicko:CKO_01054.
PATRICi20385347. VBICitKos71230_0895.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000822 Genomic DNA. Translation: ABV12197.1 . Different initiation.
RefSeqi YP_001452633.1. NC_009792.1.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 290338.CKO_01054.

Protein family/group databases

CAZyi GT20. Glycosyltransferase Family 20.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABV12197 ; ABV12197 ; CKO_01054 .
GeneIDi 5581106.
KEGGi cko:CKO_01054.
PATRICi 20385347. VBICitKos71230_0895.

Phylogenomic databases

eggNOGi COG0380.
HOGENOMi HOG000191478.
KOi K00697.
OrthoDBi EOG6CCH1M.

Enzyme and pathway databases

UniPathwayi UPA00299 .
BioCyci CKOS290338:GJ8L-1051-MONOMER.

Family and domain databases

InterProi IPR001830. Glyco_trans_20.
IPR012766. Trehalose_OtsA.
[Graphical view ]
Pfami PF00982. Glyco_transf_20. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR02400. trehalose_OtsA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-895 / CDC 4225-83 / SGSC4696.

Entry informationi

Entry nameiOTSA_CITK8
AccessioniPrimary (citable) accession number: A8AFD4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: September 2, 2008
Last modified: October 1, 2014
This is version 36 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3