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A8AFD4

- OTSA_CITK8

UniProt

A8AFD4 - OTSA_CITK8

Protein

Alpha,alpha-trehalose-phosphate synthase [UDP-forming]

Gene

otsA

Organism
Citrobacter koseri (strain ATCC BAA-895 / CDC 4225-83 / SGSC4696)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 36 (01 Oct 2014)
      Sequence version 2 (02 Sep 2008)
      Previous versions | rss
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    Functioni

    Catalyzes the transfer of glucose from UDP-glucose to glucose-6-phosphate to form alpha,alpha-1,1 trehalose-6-phosphate. Acts with retention of the anomeric configuration of the UDP-sugar donor By similarity.By similarity

    Catalytic activityi

    UDP-glucose + D-glucose 6-phosphate = UDP + alpha,alpha-trehalose 6-phosphate.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei10 – 101Glucose-6-phosphateBy similarity
    Binding sitei76 – 761Glucose-6-phosphateBy similarity
    Sitei85 – 851Involved in alpha anomer selectivityBy similarity
    Binding sitei130 – 1301Glucose-6-phosphateBy similarity
    Sitei155 – 1551Involved in alpha anomer selectivityBy similarity
    Binding sitei262 – 2621UDP-glucoseBy similarity
    Binding sitei267 – 2671UDP-glucoseBy similarity
    Binding sitei300 – 3001Glucose-6-phosphateBy similarity
    Binding sitei339 – 3391UDP-glucose; via amide nitrogen and carbonyl oxygenBy similarity

    GO - Molecular functioni

    1. alpha,alpha-trehalose-phosphate synthase (UDP-forming) activity Source: UniProtKB-EC

    GO - Biological processi

    1. trehalose biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Enzyme and pathway databases

    BioCyciCKOS290338:GJ8L-1051-MONOMER.
    UniPathwayiUPA00299.

    Protein family/group databases

    CAZyiGT20. Glycosyltransferase Family 20.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alpha,alpha-trehalose-phosphate synthase [UDP-forming] (EC:2.4.1.15)
    Alternative name(s):
    Osmoregulatory trehalose synthesis protein A
    Trehalose-6-phosphate synthase
    UDP-glucose-glucosephosphate glucosyltransferase
    Gene namesi
    Name:otsA
    Ordered Locus Names:CKO_01054
    OrganismiCitrobacter koseri (strain ATCC BAA-895 / CDC 4225-83 / SGSC4696)
    Taxonomic identifieri290338 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeCitrobacter
    ProteomesiUP000008148: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 473473Alpha,alpha-trehalose-phosphate synthase [UDP-forming]PRO_0000348890Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer.By similarity

    Protein-protein interaction databases

    STRINGi290338.CKO_01054.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni21 – 222UDP-glucose bindingBy similarity
    Regioni365 – 3695UDP-glucose bindingBy similarity

    Sequence similaritiesi

    Belongs to the glycosyltransferase 20 family.Curated

    Phylogenomic databases

    eggNOGiCOG0380.
    HOGENOMiHOG000191478.
    KOiK00697.
    OrthoDBiEOG6CCH1M.

    Family and domain databases

    InterProiIPR001830. Glyco_trans_20.
    IPR012766. Trehalose_OtsA.
    [Graphical view]
    PfamiPF00982. Glyco_transf_20. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR02400. trehalose_OtsA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    A8AFD4-1 [UniParc]FASTAAdd to Basket

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    MGRLVVVSNR IAPPDNKGGA GGLAVGVLGA LKAAGGLWFG WSGETGNEDK    50
    PLKKVTRGNM TWASFNLSEQ DYEEYYCQFS NAVLWPAFHY RLDLVQFQRP 100
    AWEGYSRVNA LLADKLLPLL KDDDIIWVHD YHLLPFASEL RKRGVNNRIG 150
    FFLHIPFPTP EIFNALPPHD TLLEQLCDFD LLGFQTENDR LAFLDSLSSQ 200
    TRVTTRGSKS HSAWGKSFRT EVYPIGIEPD EIAQQASGPL PPKLAQLKAE 250
    LKNVQNIFSV ERLDYSKGLP ERFQAYEALL EKYPQHHGKI RYTQIAPTSR 300
    GEVQAYQDIR HQLETEAGRI NGKYGQLGWT PLYYLNQHFD RKLLMKVFRY 350
    SDVGLVTPLR DGMNLVAKEF VAAQDPANPG VLVLSQFAGA ANELTSALIV 400
    NPYDRDDVAV ALHRALTMPL AERISRHAEM LETIIKNDIN HWQQRFIRDL 450
    KDVAPRSAET LHRNKVATFP KLA 473
    Length:473
    Mass (Da):53,476
    Last modified:September 2, 2008 - v2
    Checksum:i0A46A76686A24EC6
    GO

    Sequence cautioni

    The sequence ABV12197.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000822 Genomic DNA. Translation: ABV12197.1. Different initiation.
    RefSeqiYP_001452633.1. NC_009792.1.

    Genome annotation databases

    EnsemblBacteriaiABV12197; ABV12197; CKO_01054.
    GeneIDi5581106.
    KEGGicko:CKO_01054.
    PATRICi20385347. VBICitKos71230_0895.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000822 Genomic DNA. Translation: ABV12197.1 . Different initiation.
    RefSeqi YP_001452633.1. NC_009792.1.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 290338.CKO_01054.

    Protein family/group databases

    CAZyi GT20. Glycosyltransferase Family 20.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABV12197 ; ABV12197 ; CKO_01054 .
    GeneIDi 5581106.
    KEGGi cko:CKO_01054.
    PATRICi 20385347. VBICitKos71230_0895.

    Phylogenomic databases

    eggNOGi COG0380.
    HOGENOMi HOG000191478.
    KOi K00697.
    OrthoDBi EOG6CCH1M.

    Enzyme and pathway databases

    UniPathwayi UPA00299 .
    BioCyci CKOS290338:GJ8L-1051-MONOMER.

    Family and domain databases

    InterProi IPR001830. Glyco_trans_20.
    IPR012766. Trehalose_OtsA.
    [Graphical view ]
    Pfami PF00982. Glyco_transf_20. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR02400. trehalose_OtsA. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC BAA-895 / CDC 4225-83 / SGSC4696.

    Entry informationi

    Entry nameiOTSA_CITK8
    AccessioniPrimary (citable) accession number: A8AFD4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 2, 2008
    Last sequence update: September 2, 2008
    Last modified: October 1, 2014
    This is version 36 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3