A8ADQ9 (PDXB_CITK8) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Erythronate-4-phosphate dehydrogenase
EC=1.1.1.290

Gene names

Name:	pdxB
Ordered Locus Names:	CKO_00466

Organism

Citrobacter koseri (strain ATCC BAA-895 / CDC 4225-83 / SGSC4696) [Complete proteome] [HAMAP]

Taxonomic identifier

290338 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Citrobacter

Protein attributes

Sequence length	378 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the oxidation of erythronate-4-phosphate to 3-hydroxy-2-oxo-4-phosphonooxybutanoate By similarity. HAMAP MF_01825
Catalytic activity	4-phospho-D-erythronate + NAD+ = (3R)-3-hydroxy-2-oxo-4-phosphonooxybutanoate + NADH. HAMAP MF_01825
Pathway	Cofactor biosynthesis; pyridoxine 5'-phosphate biosynthesis; pyridoxine 5'-phosphate from D-erythrose 4-phosphate: step 2/5. HAMAP MF_01825
Subunit structure	Homodimer By similarity. HAMAP MF_01825
Subcellular location	Cytoplasm Potential HAMAP MF_01825.
Sequence similarities	Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. PdxB subfamily.

Ontologies

Keywords
Biological process	Pyridoxine biosynthesis
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	pyridoxine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	4-phosphoerythronate dehydrogenase activity Inferred from electronic annotation. Source: EC NAD binding Inferred from electronic annotation. Source: InterPro protein dimerization activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 378	378	Erythronate-4-phosphate dehydrogenase HAMAP MF_01825		PRO_1000088414
Sites
	Active site	208	1	By similarity
	Active site	237	1	By similarity
	Active site	254	1	Proton donor By similarity
	Binding site	45	1	Substrate By similarity
	Binding site	66	1	Substrate By similarity
	Binding site	146	1	NAD By similarity
	Binding site	175	1	NAD; via carbonyl oxygen By similarity
	Binding site	232	1	NAD By similarity
	Binding site	257	1	NAD; via amide nitrogen By similarity
	Binding site	258	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	A8ADQ9 [UniParc]. Last modified October 23, 2007. Version 1. Checksum: 92131F4B731A0B15	FASTA	378	41,017
	10 20 30 40 50 60 MKILVDENMP YARELFSRLG EVKAVPGRPI PVAELDDADA LMVRSVTKVN EALLGGKSIK 70 80 90 100 110 120 FVGTATAGTD HVDEAWLKQA GVGFSAAPGC NAIAVVEYVF SALLMLAERD GFALSDRTVG 130 140 150 160 170 180 IVGVGNVGAR LQARLEALGI RTLLCDPPRA DRGDEGDFRS LDELVQEADI LTFHTPLYKE 190 200 210 220 230 240 GPYKTLHLAD EALIGRLKPG TILINACRGP VVDNTALLAR LNAGQSLSVV LDVWEGEPDL 250 260 270 280 290 300 NVALLEKIDI GTSHIAGYTL EGKARGTTQV FEAYSTFIGR AQKVALDTLL PAPEFGRITL 310 320 330 340 350 360 HGPLDQPTLK RLAHLVYDVR RDDAPLRKVA GIPGEFDKLR KNYLERREWS SLYVMCDDAS 370 AATLLHKLGF NAVHHPAH

References

[1]

The Citrobacter koseri Genome Sequencing Project McClelland M., Sanderson E.K., Porwollik S., Spieth J., Clifton W.S., Latreille P., Courtney L., Wang C., Pepin K., Bhonagiri V., Nash W., Johnson M., Thiruvilangam P., Wilson R. Submitted (AUG-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-895 / CDC 4225-83 / SGSC4696.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000822 Genomic DNA. Translation: ABV11622.1.
RefSeq	YP_001452058.1. NC_009792.1.
3D structure databases
ProteinModelPortal	A8ADQ9.
ModBase	Search...
Protein-protein interaction databases
STRING	A8ADQ9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5584431.
GenomeReviews	Gene locus CKO_00466 in contig CP000822_GR.
KEGG	cko:CKO_00466.
PATRIC	20384322. VBICitKos71230_0392.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0111.
HOGENOM	HBG289972.
OMA	SAPGCNA.
ProtClustDB	PRK15438.
Family and domain databases
HAMAP	MF_01825. PdxB. [Tree]
InterPro	IPR006139. D-isomer_2_OHA_DH_cat_dom. IPR006140. D-isomer_2_OHA_DH_NAD-bd. IPR020921. Erythronate-4-P_DHase. IPR024531. Erythronate-4-P_DHase_dimer. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 2 hits.
KO	K03473.
PANTHER	PTHR10996:SF4. Erythronate-4-P_DHase. 1 hit.
Pfam	PF00389. 2-Hacid_dh. 1 hit. PF02826. 2-Hacid_dh_C. 1 hit. PF11890. DUF3410. 1 hit. [Graphical view]
PROSITE	PS00065. D_2_HYDROXYACID_DH_1. 1 hit. PS00670. D_2_HYDROXYACID_DH_2. False negative. PS00671. D_2_HYDROXYACID_DH_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PDXB_CITK8

Accession

Primary (citable) accession number: A8ADQ9

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	October 23, 2007
Last modified:	January 25, 2012
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program