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Protein

tRNA (cytidine/uridine-2'-O-)-methyltransferase TrmJ

Gene

trmJ

Organism
Citrobacter koseri (strain ATCC BAA-895 / CDC 4225-83 / SGSC4696)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the formation of 2'O-methylated cytidine (Cm32) or 2'O-methylated uridine (Um32) at position 32 in tRNA.By similarity

Catalytic activityi

S-adenosyl-L-methionine + cytidine(32) in tRNA = S-adenosyl-L-homocysteine + 2'-O-methylcytidine(32) in tRNA.By similarity
S-adenosyl-L-methionine + uridine(32) in tRNA = S-adenosyl-L-homocysteine + 2'-O-methyluridine(32) in tRNA.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei114S-adenosyl-L-methionine; via amide nitrogenBy similarity1
Binding sitei134S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygenBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processtRNA processing
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciCKOS290338:G1G9Z-228-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (cytidine/uridine-2'-O-)-methyltransferase TrmJBy similarity (EC:2.1.1.200By similarity)
Alternative name(s):
tRNA (cytidine(32)/uridine(32)-2'-O)-methyltransferaseBy similarity
tRNA Cm32/Um32 methyltransferaseBy similarity
Gene namesi
Name:trmJ
Ordered Locus Names:CKO_00249
OrganismiCitrobacter koseri (strain ATCC BAA-895 / CDC 4225-83 / SGSC4696)
Taxonomic identifieri290338 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeCitrobacter
Proteomesi
  • UP000008148 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm By similarity

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003138511 – 243tRNA (cytidine/uridine-2'-O-)-methyltransferase TrmJAdd BLAST243

Proteomic databases

PRIDEiA8AD50

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi290338.CKO_00249

Structurei

3D structure databases

ProteinModelPortaliA8AD50
SMRiA8AD50
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni79 – 81S-adenosyl-L-methionine bindingBy similarity3
Regioni141 – 143S-adenosyl-L-methionine bindingBy similarity3

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105CVS Bacteria
COG0565 LUCA
HOGENOMiHOG000229628
KOiK15396
OMAiPIMNLSH
OrthoDBiPOG091H03LF

Family and domain databases

Gene3Di3.40.1280.10, 1 hit
InterProiView protein in InterPro
IPR029028 Alpha/beta_knot_MTases
IPR004384 rRNA_MeTrfase_TrmH_1
IPR001537 SpoU_MeTrfase
IPR029026 tRNA_m1G_MTases_N
PANTHERiPTHR42786 PTHR42786, 1 hit
PfamiView protein in Pfam
PF00588 SpoU_methylase, 1 hit
PIRSFiPIRSF004808 LasT, 1 hit
SUPFAMiSSF75217 SSF75217, 1 hit
TIGRFAMsiTIGR00050 rRNA_methyl_1, 1 hit

Sequencei

Sequence statusi: Complete.

A8AD50-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLQNIRIVLV ETSHTGNMGS VARAMKTMGL TNLWLVNPLV KPDSQAIALA
60 70 80 90 100
AGASDVIGNA QIVDTLDDAL AGCSLVVGTS ARSRTLPWPM LDPRECGLKS
110 120 130 140 150
VAEAVNTPVA LVFGRERVGL TNDELQKCHY HVAIAANPEY SSLNLAMAVQ
160 170 180 190 200
VIAYEVRMAW LATQENDETA EHEETPYPLV DDLERFYGHL EQTLLSTGFI
210 220 230 240
REGHPGQVMN KLRRLFTRAR PESQELNILR GILASIEQQN KGK
Length:243
Mass (Da):26,687
Last modified:October 23, 2007 - v1
Checksum:iA6B851ABBC16C90B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000822 Genomic DNA Translation: ABV11413.1
RefSeqiWP_012131245.1, NC_009792.1

Genome annotation databases

EnsemblBacteriaiABV11413; ABV11413; CKO_00249
KEGGicko:CKO_00249

Similar proteinsi

Entry informationi

Entry nameiTRMJ_CITK8
AccessioniPrimary (citable) accession number: A8AD50
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: October 23, 2007
Last modified: April 25, 2018
This is version 56 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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