A8ACX7 (METE_CITK8) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase
EC=2.1.1.14

Alternative name(s):
Cobalamin-independent methionine synthase
Methionine synthase, vitamin-B12 independent isozyme

Gene names

Name:	metE
Ordered Locus Names:	CKO_00171

Organism

Citrobacter koseri (strain ATCC BAA-895 / CDC 4225-83 / SGSC4696) [Complete proteome] [HAMAP]

Taxonomic identifier

290338 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Citrobacter

Protein attributes

Sequence length	753 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation By similarity. HAMAP MF_00172
Catalytic activity	5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine. HAMAP MF_00172
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00172
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetE route): step 1/1. HAMAP MF_00172
Sequence similarities	Belongs to the vitamin-B12 independent methionine synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Domain	Repeat
Ligand	Metal-binding Zinc
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 753	753	5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase HAMAP MF_00172		PRO_1000017238
Sites
	Metal binding	641	1	Zinc By similarity
	Metal binding	643	1	Zinc By similarity
	Metal binding	726	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	A8ACX7 [UniParc]. Last modified October 23, 2007. Version 1. Checksum: 09E8295976718215	FASTA	753	84,488
	10 20 30 40 50 60 MTIRNHTLGF PRVGLRRELK KAQESYWAGN ATREDLLATG RELRARHWDQ QKQAGIDLLP 70 80 90 100 110 120 VGDFAWYDHV LTTSLLLGNV PARHQNSDGS VDIDTLFRIG RGRAPTGEPA AAAEMTKWFN 130 140 150 160 170 180 TNYHYMVPEF VKGQQFKLTW TQLLDEVDEA LALGHKVKPV LLGPVTYLWL GKVKGESFDR 190 200 210 220 230 240 LSLLNDILPV YKQVLTELAK RGVEWVQIDE PALVLELPQE WLDAYKPAYD ALQGQVKLLL 250 260 270 280 290 300 TTYFEGVTPN LDTIAALPVQ GLHVDLVHGK DDVAELHKRL PSDWLLSAGL VNGRNVWRAD 310 320 330 340 350 360 LTEKYAQIKD IVGKRDLWVA SSCSLLHSPI DLSVETRLDA EVKSWFAFAL QKCGELALLR 370 380 390 400 410 420 DALNSGDTAK LEAWSAPIQA RRHSTRVHNA AVEKRLAAIT AQDSQRASPY AARAQAQRNR 430 440 450 460 470 480 FNLPSWPTTT IGSFPQTTEI RGLRLDFKKG NLDANHYRTG IAEHIKQAIV EQERLGLDVL 490 500 510 520 530 540 VHGEAERNDM VEYFGEHLDG FVFTQNGWVQ SYGSRCVKPP VVIGDVSRPE PITVEWAKYA 550 560 570 580 590 600 QSLTDKPVKG MLTGPVTILC WSFPREDVSR ETIAKQIALA LRDEVADLEA AGIGIIQIDE 610 620 630 640 650 660 PALREGLPLR RSDWDAYLQW GVEAFRINAA VAKDDTQIHT HMCYCEFNDI MDSIAALDAD 670 680 690 700 710 720 VITIETSRSD MELLESFEEF EYPNEIGPGV YDIHSPNVPS VEWIEALLNK AAQRIPAERL 730 740 750 WVNPDCGLKT RGWPETRAAL ANMVKAAQNL RQA

References

[1]

The Citrobacter koseri Genome Sequencing Project McClelland M., Sanderson E.K., Porwollik S., Spieth J., Clifton W.S., Latreille P., Courtney L., Wang C., Pepin K., Bhonagiri V., Nash W., Johnson M., Thiruvilangam P., Wilson R. Submitted (AUG-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-895 / CDC 4225-83 / SGSC4696.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000822 Genomic DNA. Translation: ABV11340.1.
RefSeq	YP_001451776.1. NC_009792.1.
3D structure databases
ProteinModelPortal	A8ACX7.
ModBase	Search...
Protein-protein interaction databases
STRING	A8ACX7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5582181.
GenomeReviews	Gene locus CKO_00171 in contig CP000822_GR.
KEGG	cko:CKO_00171.
PATRIC	20383804. VBICitKos71230_0144.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0620.
HOGENOM	HBG287495.
OMA	RNIWRAN.
ProtClustDB	PRK05222.
Family and domain databases
HAMAP	MF_00172. Meth_synth. [Tree]
InterPro	IPR013215. Cbl-indep_Met_Synth_N. IPR006276. Cobalamin-indep_Met_synthase. IPR002629. Methionine_synth. [Graphical view]
KO	K00549.
Pfam	PF08267. Meth_synt_1. 1 hit. PF01717. Meth_synt_2. 1 hit. [Graphical view]
PIRSF	PIRSF000382. MeTrfase_B12_ind. 1 hit.
TIGRFAMs	TIGR01371. Met_syn_B12ind. 1 hit.
ProtoNet	Search...

Entry information

Entry name

METE_CITK8

Accession

Primary (citable) accession number: A8ACX7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 23, 2007
Last modified:	January 25, 2012
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program