A8ABX6 (PURL_IGNH4) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylformylglycinamidine synthase 2
EC=6.3.5.3

Alternative name(s):
Phosphoribosylformylglycinamidine synthase II
Short name=FGAM synthase II

Gene names

Name:	purL
Ordered Locus Names:	Igni_1252

Organism

Ignicoccus hospitalis (strain KIN4/I / DSM 18386 / JCM 14125) [Complete proteome] [HAMAP]

Taxonomic identifier

453591 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Desulfurococcales › Desulfurococcaceae › Ignicoccus

Protein attributes

Sequence length	713 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate. HAMAP MF_00420
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2. HAMAP MF_00420
Subunit structure	Heterodimer of two subunits, PurQ and PurL.
Subcellular location	Cytoplasm By similarity HAMAP MF_00420.
Sequence similarities	Belongs to the FGAMS family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylformylglycinamidine synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 713	713	Phosphoribosylformylglycinamidine synthase 2 HAMAP MF_00420		PRO_1000050311
Regions
	Nucleotide binding	88 – 99	12	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	A8ABX6 [UniParc]. Last modified October 23, 2007. Version 1. Checksum: 460C82906F3CE020	FASTA	713	77,918
	10 20 30 40 50 60 MPLTEEELKL IEETLGRKPN QVELAMFEAQ WSEHCSYKSS RKHLRKLKMD SPWVVKGGDA 70 80 90 100 110 120 AVVDFGSVYV AFRIESHNHP SAVDPYNGAA TGVGGIIRDI LSSGAKPIAL LDDLIFGDLD 130 140 150 160 170 180 ENLVKWHVKG VVKGISDYGN RVGVPTVGGE TWFDPDFTRN PMVSVACVGV CPKDKLINGT 190 200 210 220 230 240 PRPGDLIVIA GNYTGKDGLL GSSFASKNLE EGVEEEYAAI QVPDPLMEKL LIDSILEMRD 250 260 270 280 290 300 ERLLVFVKDL GGGGLATALS EVAASFGLGV EARLDALHLR EPMEAWEIVV SESQERMMLV 310 320 330 340 350 360 IRPEDLERAK KVLEKYDVPY SVIGKFTDTG RVVLYYNGEK VADLPAKFLA EGPELDRPYE 370 380 390 400 410 420 RPKWHLELEL LPPLPEVDLG DAIRKVLSSP NVASKRWIYE QYDHEVQIRT VVKPGEGDAA 430 440 450 460 470 480 VLRLLEDPPK GIAVATDSNP RYTFLDPLWG AADVFLEAYR NVVASGARPL AAVDQVDAGS 490 500 510 520 530 540 PERPDRFWFF VRMIDGLAWV EREVDVPIVG GKVSFYNEDD VTGKQIKPTV MITMIGKVEN 550 560 570 580 590 600 VYNAKRARAE EGDLLVLVGE TFPELGGSEF LWSVHKLVRG KPPVPRPSTE ARVAERILKA 610 620 630 640 650 660 IKVSGVTGVH DVSVGGLAAA VAELAKGFGA TLELDKVKGP WKRPEEALFS ESGARYLLAV 670 680 690 700 710 KPEAAEEVLK VTGGTVVGKV GGGPLRVLLN GKEVASVDDF EDLLENGMTS RLV

References

[1]

"A genomic analysis of the archaeal system Ignicoccus hospitalis-Nanoarchaeum equitans." Podar M., Anderson I., Makarova K.S., Elkins J.G., Ivanova N., Wall M.A., Lykidis A., Mavromatis K., Sun H., Hudson M.E., Chen W., Deciu C., Hutchison D., Eads J.R., Anderson A., Fernandes F., Szeto E., Lapidus A. , Kyrpides N.C., Saier M.H. Jr., Richardson P.M., Rachel R., Huber H., Eisen J.A., Koonin E.V., Keller M., Stetter K.O. Genome Biol. 9:R158.1-R158.18(2008) [PubMed: 19000309] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: KIN4/I / DSM 18386 / JCM 14125.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000816 Genomic DNA. Translation: ABU82428.1.
RefSeq	YP_001435835.1. NC_009776.1.
3D structure databases
ProteinModelPortal	A8ABX6.
ModBase	Search...
Protein-protein interaction databases
STRING	A8ABX6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5562767.
GenomeReviews	Gene locus Igni_1252 in contig CP000816_GR.
KEGG	iho:Igni_1252.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG04101.
HOGENOM	HBG311214.
OMA	YGNSFGV.
ProtClustDB	PRK01213.
Enzyme and pathway databases
BioCyc	IHOS453591:IGNI_1252-MONOMER.
Family and domain databases
HAMAP	MF_00420. PurL_2. [Tree]
InterPro	IPR000728. AIR_synth. IPR010918. AIR_synth_C. IPR010074. PRibForGlyAmidine_synth_II. IPR016188. PurM_N-like. [Graphical view]
KO	K01952.
Pfam	PF00586. AIRS. 2 hits. PF02769. AIRS_C. 2 hits. [Graphical view]
SUPFAM	SSF56042. AIR_synth_C. 2 hits. SSF55326. PurM_N-like. 2 hits.
TIGRFAMs	TIGR01736. FGAM_synth_II. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PURL_IGNH4

Accession

Primary (citable) accession number: A8ABX6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 23, 2007
Last modified:	January 25, 2012
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program