A8AB39 (ILVD_IGNH4) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dihydroxy-acid dehydratase
Short name=DAD
EC=4.2.1.9

Gene names

Name:	ilvD
Ordered Locus Names:	Igni_0961

Organism

Ignicoccus hospitalis (strain KIN4/I / DSM 18386 / JCM 14125) [Complete proteome] [HAMAP]

Taxonomic identifier

453591 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Desulfurococcales › Desulfurococcaceae › Ignicoccus

Protein attributes

Sequence length	552 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O. HAMAP MF_00012
Cofactor	Binds 1 4Fe-4S cluster Potential.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4. HAMAP MF_00012 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4. HAMAP MF_00012
Sequence similarities	Belongs to the IlvD/Edd family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	branched chain family amino acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW dihydroxy-acid dehydratase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 552	552	Dihydroxy-acid dehydratase HAMAP MF_00012		PRO_1000000994
Sites
	Metal binding	119	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	190	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	A8AB39 [UniParc]. Last modified October 23, 2007. Version 1. Checksum: 122E8EBA856A4735	FASTA	552	59,728
	10 20 30 40 50 60 MKRSDVFLRP EYAPHRALWR ASGLIDEELR RPLIGVANSW NEIVPGHVHL DKVAEAVKAG 70 80 90 100 110 120 IRMAGGTPLE FGTIAVCDGI AMGHEGMRYS LPSREVIADT VEIMVEAHRL DAVVMVTNCD 130 140 150 160 170 180 KITPGFLLAA ARLEVPVILI NGGPMMPGVY GKERIDFKDL MERMNVLIKE GRTEELRKLE 190 200 210 220 230 240 ESALPGPGSC AGLFTANTMN MLSEAMGLML PGASTVPAVE ARRLWYAKLT GMRIVKMVEE 250 260 270 280 290 300 GLTPDKILTR KALENAIAVD MALGGSTNSV LHLEALAYEL GIDLPLEVFD EISRKVPHIA 310 320 330 340 350 360 SISPSGRHFV VDLDRAGGIP AVLKELGEAG LIHKDALTVT GKTVWENVKD AAVLDREVIR 370 380 390 400 410 420 PLDNPYSPFG GLAILKGSLA PNGAVVKASA VKRELWKFKG VARVFDREED AVKAIRGGEI 430 440 450 460 470 480 EPGTVIVIRY EGPRGGPGMR EMLTATAAVM ALGLGDKVAL VTDGRFSGAT RGPAIGHVSP 490 500 510 520 530 540 EAAAGGPIAL VQDGDEIVID IEKRRLDLLV DEKELEERRA RWKPKVKPLR RGILRRYAKM 550 ALSADKGGAL EY

References

[1]

"A genomic analysis of the archaeal system Ignicoccus hospitalis-Nanoarchaeum equitans." Podar M., Anderson I., Makarova K.S., Elkins J.G., Ivanova N., Wall M.A., Lykidis A., Mavromatis K., Sun H., Hudson M.E., Chen W., Deciu C., Hutchison D., Eads J.R., Anderson A., Fernandes F., Szeto E., Lapidus A. , Kyrpides N.C., Saier M.H. Jr., Richardson P.M., Rachel R., Huber H., Eisen J.A., Koonin E.V., Keller M., Stetter K.O. Genome Biol. 9:R158.1-R158.18(2008) [PubMed: 19000309] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: KIN4/I / DSM 18386 / JCM 14125.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000816 Genomic DNA. Translation: ABU82141.1.
RefSeq	YP_001435548.1. NC_009776.1.
3D structure databases
ProteinModelPortal	A8AB39.
ModBase	Search...
Protein-protein interaction databases
STRING	A8AB39.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5562278.
GenomeReviews	Gene locus Igni_0961 in contig CP000816_GR.
KEGG	iho:Igni_0961.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG04340.
HOGENOM	HBG671001.
OMA	HRALLKG.
Enzyme and pathway databases
BioCyc	IHOS453591:IGNI_0961-MONOMER.
Family and domain databases
HAMAP	MF_00012. IlvD. [Tree]
InterPro	IPR015928. Aconitase/3IPM_dehydase_swvl. IPR004404. DihydroxyA_deHydtase. IPR000581. DiOHA_6PGluconate_deHydtase. IPR020558. DiOHA_6PGluconate_deHydtase_CS. [Graphical view]
KO	K01687.
PANTHER	PTHR21000. ILVD_EDD_family. 1 hit.
Pfam	PF00920. ILVD_EDD. 1 hit. [Graphical view]
SUPFAM	SSF52016. Aconitase/3IPM_dehydase_swvl. 1 hit.
TIGRFAMs	TIGR00110. IlvD. 1 hit.
PROSITE	PS00886. ILVD_EDD_1. 1 hit. PS00887. ILVD_EDD_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ILVD_IGNH4

Accession

Primary (citable) accession number: A8AB39

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 23, 2007
Last modified:	November 16, 2011
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program