Reviewed, UniProtKB/Swiss-Prot A8AB20 (DNLI_IGNH4)

Names and origin

Protein names

Recommended name:
    DNA ligase
    EC=6.5.1.1
Alternative name(s):
    Polydeoxyribonucleotide synthase [ATP]

Gene names

Name:	lig
Ordered Locus Names:	Igni_0942

Organism

Ignicoccus hospitalis (strain KIN4/I / DSM 18386 / JCM 14125) [Complete proteome] [HAMAP]

Taxonomic identifier

453591 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Desulfurococcales › Desulfurococcaceae › Ignicoccus

Protein attributes

Sequence length	594 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This protein seals, during DNA replication, DNA recombination and DNA repair, nicks in double-stranded DNA By similarity.
Catalytic activity	ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).
Sequence similarities	Belongs to the ATP-dependent DNA ligase family.

Ontologies

Keywords
Biological process	Cell cycle Cell division DNA damage DNA recombination DNA repair DNA replication
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA recombination Inferred from electronic annotation. Source: HAMAP DNA repair Inferred from electronic annotation. Source: HAMAP DNA replication Inferred from electronic annotation. Source: HAMAP cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP DNA binding Inferred from electronic annotation. Source: InterPro DNA ligase (ATP) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 594	594	DNA ligase		PRO_1000049867
Sites
	Active site	258	1	N6-AMP-lysine intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	A8AB20-1 [UniParc]. Last modified October 23, 2007. Version 1. Checksum: 2AB8030EEC27B803	FASTA	594	66,459
	10 20 30 40 50 60 MKFSDVVDAL ERLERTTSRT QIVAILTNLF KKVIEENKDI IDKVVYFIQG KLWPDWYGYP 70 80 90 100 110 120 EIGIGEKGII KAISLAANVK EKEVEGLYKQ LGDLGLVAER LMAKAPKGGL MMFVKKKEEL 130 140 150 160 170 180 TFEKVYETLK RIAFMQGEGS RDLKIKTLAG LLKEASPKEA KYIVRFVQGK LRLGVGDASI 190 200 210 220 230 240 IEALAHVAGT TKDVVERAYN LRADLGAVAK IAVTEGPEAL KRVRPKPGVP VRPMLAERLN 250 260 270 280 290 300 DPKEILKKLG GKGLAEYKYD GERAQIHLLP DGKVVIFSRR LENITRSYPD VVQYAKSGLK 310 320 330 340 350 360 AKEAIVEGEI IAVNPETGEP RPFQELMRRR RKHDVALAMS EIPVNVKLFD IIYVDGEDMT 370 380 390 400 410 420 NKPLPLRRKR LEEVVEESEE FSLSTAKLVS TPEELEQFFH QSISEGHEGL VVKAVHDKSV 430 440 450 460 470 480 YQAGARGWLW IKYKKDYKSE MVEPVDLVVV GAFYGRGRRG GTFGALLVAG YDEKRDAFAT 490 500 510 520 530 540 VCKVGSGFSD EELARLPELL KPYISETKPP RVISNVKPDV WVRPALVAEI IGAEITLSPI 550 560 570 580 590 HTCAKDEVSA GSGLAIRFPR FIRWRPDKGP EDATTCGEIV EMYKSRLKKV EEPT

References

[1]

"Unicellular symbiosis at high temperature: genomic insights into the Ignicoccus hospitalis - Naoarchaeum equitans relationship." Podar M., Anderson I., Ivanova N., Wall M., Mavrommatis K., Lykidis A., Chen J., Hudson M., Deciu C., Hutchison D., Eads J., Fernandes F., Tamang D., Szeto E., Land M., Lapidus A., Kyrpides N., Chang C. , Saier M., Eisen J., Rachel R., Huber H., Keller M., Richardson P., Stetter K. Submitted (AUG-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000816 Genomic DNA. Translation: ABU82122.1.
RefSeq	YP_001435529.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5562329.
GenomeReviews	Gene locus Igni_0942 in contig CP000816_GR.
KEGG	iho:Igni_0942.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_00407. [Tree]
InterPro	IPR000977. DNA_ligase. IPR012309. DNA_ligase_A_C. IPR012310. DNA_ligase_A_M. IPR012308. DNA_ligase_A_N. IPR016059. DNA_ligase_CS. IPR012340. NA-bd_OB-fold. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
Pfam	PF04679. DNA_ligase_A_C. 1 hit. PF01068. DNA_ligase_A_M. 1 hit. PF04675. DNA_ligase_A_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR00574. dnl1. 1 hit.
PROSITE	PS00697. DNA_LIGASE_A1. 1 hit. PS00333. DNA_LIGASE_A2. 1 hit. PS50160. DNA_LIGASE_A3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DNLI_IGNH4

Accession

Primary (citable) accession number: A8AB20

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 23, 2007
Last modified:	November 25, 2008
This is version 11 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)