A8A9X7 (RIFK_IGNH4) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Riboflavin kinase
Short name=RFK
EC=2.7.1.161

Alternative name(s):
CTP-dependent riboflavin kinase
CTP:riboflavin 5'-phosphotransferase
Flavokinase

Gene names

Name:	ribK
Ordered Locus Names:	Igni_0547

Organism

Ignicoccus hospitalis (strain KIN4/I / DSM 18386 / JCM 14125) [Complete proteome] [HAMAP]

Taxonomic identifier

453591 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Desulfurococcales › Desulfurococcaceae › Ignicoccus

Protein attributes

Sequence length	179 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the CTP-dependent phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN) By similarity. HAMAP MF_01285
Catalytic activity	CTP + riboflavin = CDP + FMN. HAMAP MF_01285
Cofactor	Binds 1 magnesium ion per subunit By similarity. HAMAP MF_01285
Pathway	Cofactor biosynthesis; FMN biosynthesis; FMN from riboflavin (CTP route): step 1/1. HAMAP MF_01285
Sequence similarities	Belongs to the archaeal riboflavin kinase family.

Ontologies

Keywords
Ligand	FMN Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	riboflavin biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW riboflavin kinase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 179	179	Riboflavin kinase HAMAP MF_01285		PRO_0000322067
Regions
	Nucleotide binding	61 – 66	6	CDP By similarity
	Nucleotide binding	160 – 163	4	CDP By similarity
Sites
	Metal binding	90	1	Magnesium By similarity
	Metal binding	92	1	Magnesium By similarity
	Binding site	147	1	FMN By similarity
	Binding site	155	1	FMN By similarity

Sequences

	Sequence		Length	Mass (Da)
	A8A9X7 [UniParc]. Last modified October 23, 2007. Version 1. Checksum: FDD41DD0E46F0E6B	FASTA	179	20,113
	10 20 30 40 50 60 MLAALYYVLK GDPYKALKED LESLKKAKLL TEDLRITEKG MELIQQLISK PISLKGKVVS 70 80 90 100 110 120 GDGEGRYYLS LEGYRRQVRE KLGFDPFPGT LNVLLDPTST EKKSTLMFKR PIILKGFTEN 130 140 150 160 170 GKRYGEVLAF PARVSGVEAA LVIPLKTHHP PEIIELISPV ELRKALKLKD GDEVEVLVY

References

[1]

"A genomic analysis of the archaeal system Ignicoccus hospitalis-Nanoarchaeum equitans." Podar M., Anderson I., Makarova K.S., Elkins J.G., Ivanova N., Wall M.A., Lykidis A., Mavromatis K., Sun H., Hudson M.E., Chen W., Deciu C., Hutchison D., Eads J.R., Anderson A., Fernandes F., Szeto E., Lapidus A. , Kyrpides N.C., Saier M.H. Jr., Richardson P.M., Rachel R., Huber H., Eisen J.A., Koonin E.V., Keller M., Stetter K.O. Genome Biol. 9:R158.1-R158.18(2008) [PubMed: 19000309] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: KIN4/I / DSM 18386 / JCM 14125.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000816 Genomic DNA. Translation: ABU81729.1.
RefSeq	YP_001435136.1. NC_009776.1.
3D structure databases
ProteinModelPortal	A8A9X7.
ModBase	Search...
Protein-protein interaction databases
STRING	A8A9X7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5562129.
GenomeReviews	Gene locus Igni_0547 in contig CP000816_GR.
KEGG	iho:Igni_0547.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG10832.
HOGENOM	HBG553349.
OMA	PYEGTLN.
Enzyme and pathway databases
BioCyc	IHOS453591:IGNI_0547-MONOMER.
Family and domain databases
HAMAP	MF_01285. Riboflavin_kinase. [Tree]
InterPro	IPR023470. Riboflavin_kinase_archaeal. IPR023602. Riboflavin_kinase_CTP-dep. IPR023465. Riboflavin_kinase_domain. [Graphical view]
Gene3D	G3DSA:2.40.30.30. Riboflavin_kinase. 1 hit.
KO	K07732.
Pfam	PF01982. CTP-dep_RFKase. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RIFK_IGNH4

Accession

Primary (citable) accession number: A8A9X7

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	October 23, 2007
Last modified:	November 16, 2011
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program