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A8A9W2 (RTCA_IGNH4) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 47. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
RNA 3'-terminal phosphate cyclase

Short name=RNA cyclase
Short name=RNA-3'-phosphate cyclase
EC=6.5.1.4
Gene names
Name:rtcA
Ordered Locus Names:Igni_0532
OrganismIgnicoccus hospitalis (strain KIN4/I / DSM 18386 / JCM 14125) [Complete proteome] [HAMAP]
Taxonomic identifier453591 [NCBI]
Taxonomic lineageArchaeaCrenarchaeotaThermoproteiDesulfurococcalesDesulfurococcaceaeIgnicoccus

Protein attributes

Sequence length353 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA-N3'PP5'A; (C) and attack of the adjacent 2'-hydroxyl on the 3'-phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing By similarity. HAMAP-Rule MF_00200

Catalytic activity

ATP + RNA 3'-terminal-phosphate = AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate. HAMAP-Rule MF_00200

Subcellular location

Cytoplasm Potential HAMAP-Rule MF_00200.

Sequence similarities

Belongs to the RNA 3'-terminal cyclase family. Type 1 subfamily.

Ontologies

Keywords
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processRNA processing

Inferred from electronic annotation. Source: InterPro

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

RNA-3'-phosphate cyclase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 353353RNA 3'-terminal phosphate cyclase HAMAP-Rule MF_00200
PRO_0000325190

Regions

Nucleotide binding289 – 2924ATP By similarity

Sites

Active site3151Tele-AMP-histidine intermediate By similarity
Binding site1001ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A8A9W2 [UniParc].

Last modified October 23, 2007. Version 1.
Checksum: F513232B42846DA7

FASTA35338,695
        10         20         30         40         50         60 
MIEIDGSFGE GGGQILRTSV ALSAVTLKPV RIFNIRAKRK NPGLRRQHMV AVKALAEMTD 

        70         80         90        100        110        120 
AEVRGLELGS TEIVFIPKTL KAGTFRFDIG TAGSVSLVLQ AVTPAALFAP GEVRVQLRGG 

       130        140        150        160        170        180 
TDVPMSPPVD YLRFVFYPLL ERFGAKTELV LKRRGHYPKG GGEVEFASRP VDSLTQWGEV 

       190        200        210        220        230        240 
ERGEVLKVRG LSHCVKLPKH VAERQAKAAE EVLKKSGLKN VDIDLEWYPP ERDPHLGPGS 

       250        260        270        280        290        300 
GIVLWAITER SLLGADSLGA RGKRAERVGE EAARKLLEDL STGKALDRHM SDMIVPYVSL 

       310        320        330        340        350 
ACGRTEVGGA ALTMHAWTHV HVVKKFLPEL EVEISGELNK PFVMRVKGVC WQR 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000816 Genomic DNA. Translation: ABU81714.1.
RefSeqYP_001435121.1. NC_009776.1.

3D structure databases

ProteinModelPortalA8A9W2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING453591.Igni_0532.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABU81714; ABU81714; Igni_0532.
GeneID5562318.
KEGGiho:Igni_0532.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0430.
HOGENOMHOG000015264.
KOK01974.
OMATTHIETC.

Enzyme and pathway databases

BioCycIHOS453591:GHYN-548-MONOMER.

Family and domain databases

Gene3D3.30.360.20. 1 hit.
3.65.10.20. 2 hits.
HAMAPMF_00200. RTC.
InterProIPR013791. RNA3'-term_phos_cycl_insert.
IPR023797. RNA3'_phos_cyclase_dom.
IPR000228. RNA3'_term_phos_cyc.
IPR017770. RNA3'_term_phos_cyc_type_1.
IPR020719. RNA3'_term_phos_cycl-like_CS.
IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b.
[Graphical view]
PANTHERPTHR11096. PTHR11096. 1 hit.
PfamPF01137. RTC. 1 hit.
PF05189. RTC_insert. 1 hit.
[Graphical view]
SUPFAMSSF52913. SSF52913. 1 hit.
SSF55205. SSF55205. 1 hit.
TIGRFAMsTIGR03399. RNA_3prim_cycl. 1 hit.
PROSITEPS01287. RTC. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRTCA_IGNH4
AccessionPrimary (citable) accession number: A8A9W2
Entry history
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: October 23, 2007
Last modified: May 14, 2014
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families