A8A8W1 (NTPA_IGNH4) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Non-canonical purine NTP pyrophosphatase
EC=3.6.1.19

Alternative name(s):
Non-standard purine NTP pyrophosphatase
Nucleoside-triphosphate diphosphatase
Nucleoside-triphosphate pyrophosphatase
Short name=NTPase

Gene names

Ordered Locus Names:

Igni_0179

Organism

Ignicoccus hospitalis (strain KIN4/I / DSM 18386 / JCM 14125) [Complete proteome] [HAMAP]

Taxonomic identifier

453591 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Desulfurococcales › Desulfurococcaceae › Ignicoccus

Protein attributes

Sequence length	188 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Pyrophosphatase that hydrolyzes non-canonical purine nucleotides such as XTP and ITP/dITP to their respective monophosphate derivatives. Might exclude non-canonical purines from DNA precursor pool, thus preventing their incorporation into DNA and avoiding chromosomal lesions By similarity. HAMAP MF_01405
Catalytic activity	A nucleoside triphosphate + H2O = a nucleotide + diphosphate. HAMAP MF_01405
Cofactor	Binds 1 divalent metal cation ion per subunit; can use either magnesium or manganese By similarity.
Subunit structure	Homodimer By similarity. HAMAP MF_01405
Sequence similarities	Belongs to the HAM1 NTPase family.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Ligand	Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	nucleotide metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleoside-triphosphatase activity Inferred from electronic annotation. Source: InterPro nucleoside-triphosphate diphosphatase activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 188	188	Non-canonical purine NTP pyrophosphatase HAMAP MF_01405		PRO_1000087379
Regions
	Region	10 – 15	6	Substrate binding By similarity
	Region	69 – 70	2	Substrate binding By similarity
Sites
	Metal binding	39	1	Magnesium or manganese By similarity
	Metal binding	69	1	Magnesium or manganese By similarity
	Binding site	148	1	Substrate By similarity
	Binding site	168	1	Substrate By similarity
	Binding site	174	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	A8A8W1 [UniParc]. Last modified October 23, 2007. Version 1. Checksum: 0121E8C356DB352C	FASTA	188	21,114
	10 20 30 40 50 60 MVSKKVYFLT SNPHKAKEVS DVLSQFSIEV VPLKGEKLEI QADSVEEVAR FAAEEAKKRF 70 80 90 100 110 120 KERPLLLEDS GLFVDALKGF PGPYSNYVYR TLGLEGLLKL MEGVEDRRAR FVCAAALVKE 130 140 150 160 170 180 DDKIVIEVGE VEGEIAYEPR GDKGFGFDPI FVPLGYEKTF AELGEEVKKR ISHRARAFMK IAKHLSGE

References

[1]

"A genomic analysis of the archaeal system Ignicoccus hospitalis-Nanoarchaeum equitans." Podar M., Anderson I., Makarova K.S., Elkins J.G., Ivanova N., Wall M.A., Lykidis A., Mavromatis K., Sun H., Hudson M.E., Chen W., Deciu C., Hutchison D., Eads J.R., Anderson A., Fernandes F., Szeto E., Lapidus A. , Kyrpides N.C., Saier M.H. Jr., Richardson P.M., Rachel R., Huber H., Eisen J.A., Koonin E.V., Keller M., Stetter K.O. Genome Biol. 9:R158.1-R158.18(2008) [PubMed: 19000309] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: KIN4/I / DSM 18386 / JCM 14125.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000816 Genomic DNA. Translation: ABU81363.1.
RefSeq	YP_001434770.1. NC_009776.1.
3D structure databases
ProteinModelPortal	A8A8W1.
SMR	A8A8W1. Positions 5-185.
ModBase	Search...
Protein-protein interaction databases
STRING	A8A8W1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5562338.
GenomeReviews	Gene locus Igni_0179 in contig CP000816_GR.
KEGG	iho:Igni_0179.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG10472.
HOGENOM	HBG697237.
OMA	VEDSGFF.
Enzyme and pathway databases
BioCyc	IHOS453591:IGNI_0179-MONOMER.
Family and domain databases
HAMAP	MF_01405. Non_canon_purine_NTPase. [Tree]
InterPro	IPR002637. Ham1p-like. IPR020922. Nucleoside-triphosphatase. [Graphical view]
KO	K02428.
PANTHER	PTHR11067. Ham1p_like. 1 hit.
Pfam	PF01725. Ham1p_like. 1 hit. [Graphical view]
TIGRFAMs	TIGR00042. TIGR00042. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NTPA_IGNH4

Accession

Primary (citable) accession number: A8A8W1

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	October 23, 2007
Last modified:	December 14, 2011
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program