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A8A770 (STHA_ECOHS) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 35. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Soluble pyridine nucleotide transhydrogenase
Short name=STH
EC=1.6.1.1
Alternative name(s):
NAD(P)(+) transhydrogenase [B-specific]
Gene names
Name:sthA
Synonyms:udhA
Ordered Locus Names:EcHS_A4196
OrganismEscherichia coli O9:H4 (strain HS) [Complete proteome] [HAMAP]
Taxonomic identifier331112 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length466 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Conversion of NADPH, generated by peripheral catabolic pathways, to NADH, which can enter the respiratory chain for energy generation By similarity. HAMAP MF_00247

Catalytic activity

NADPH + NAD+ = NADP+ + NADH. HAMAP MF_00247

Cofactor

Binds 1 FAD per subunit By similarity. HAMAP MF_00247

Subcellular location

Cytoplasm By similarity HAMAP MF_00247.

Sequence similarities

Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.

Ontologies

Keywords
   Cellular componentCytoplasm
   LigandFAD
Flavoprotein
NAD
NADP
   Molecular functionOxidoreductase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processcell redox homeostasis

Inferred from electronic annotation. Source: InterPro

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionNAD(P)+ transhydrogenase (B-specific) activity

Inferred from electronic annotation. Source: EC

flavin adenine dinucleotide binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 466466Soluble pyridine nucleotide transhydrogenase HAMAP MF_00247
PRO_1000059009

Regions

Nucleotide binding36 – 4510FAD By similarity

Sequences

Sequence LengthMass (Da)Tools
A8A770 [UniParc].

Last modified October 23, 2007. Version 1.
Checksum: 3184DB14052CB60F

FASTA46651,574
        10         20         30         40         50         60 
MPHSYDYDAI VIGSGPGGEG AAMGLVKQGA RVAVIERYQN VGGGCTHWGT IPSKALRHAV 

        70         80         90        100        110        120 
SRIIEFNQNP LYSDHSRLLR SSFADILNHA DNVINQQTRM RQGFYERNHC EILQGNARFV 

       130        140        150        160        170        180 
DEHTLALDCP DGSVETLTAE KFIIACGSRP YHPTDVDFTH PRIYDSDSIL SMHHEPRHVL 

       190        200        210        220        230        240 
IYGAGVIGCE YASIFRGMDV KVDLINTRDR LLAFLDQEMS DSLSYHFWNS GVVIRHNEEY 

       250        260        270        280        290        300 
EKIEGCDDGV IMHLKSGKKL KADCLLYANG RTGNTDSLAL QNIGLETDSR GQLKVNSMYQ 

       310        320        330        340        350        360 
TAQPHVYAVG DVIGYPSLAS AAYDQGRIAA QALVKGEATA HLIEDIPTGI YTIPEISSVG 

       370        380        390        400        410        420 
KTEQQLTAMK VPYEVGRAQF KHLARAQIVG MNVGTLKILF HRETKEILGI HCFGERAAEI 

       430        440        450        460 
IHIGQAIMEQ KGGGNTIEYF VNTTFNYPTM AEAYRVAALN GLNRLF

« Hide

References

[1]"The pangenome structure of Escherichia coli: comparative genomic analysis of E. coli commensal and pathogenic isolates."
Rasko D.A., Rosovitz M.J., Myers G.S.A., Mongodin E.F., Fricke W.F., Gajer P., Crabtree J., Sebaihia M., Thomson N.R., Chaudhuri R., Henderson I.R., Sperandio V., Ravel J.
J. Bacteriol. 190:6881-6893(2008) [PubMed: 18676672] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: HS.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000802 Genomic DNA. Translation: ABV08374.1.
RefSeqYP_001460757.1. NC_009800.1.

3D structure databases

ProteinModelPortalA8A770.
SMRA8A770. Positions 6-464.
ModBaseSearch...

Protein-protein interaction databases

STRINGA8A770.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBESCT00000054687; EBESCP00000052672; EBESCG00000053735.
GeneID5594459.
GenomeReviewsGene locus EcHS_A4196 in contig CP000802_GR.
KEGGecx:EcHS_A4196.
PATRIC18318356. VBIEscCol77814_4088.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1249.
GeneTreeEBGT00050000009315.
HOGENOMHBG515043.
OMAGEGNTIE.
ProtClustDBPRK05249.

Family and domain databases

HAMAPMF_00247. SthA.
[Tree]
InterProIPR016156. FAD/NAD-linked_Rdtase_dimer.
IPR013027. FAD_pyr_nucl-diS_OxRdtase.
IPR004099. Pyr_nucl-diS_OxRdtase_dimer.
IPR023753. Pyr_nucl-diS_OxRdtase_FAD/NAD.
IPR001327. Pyr_OxRdtase_NAD-bd_dom.
IPR022962. STH.
[Graphical view]
Gene3DG3DSA:3.30.390.30. Pyr_redox_dim. 1 hit.
KOK00322.
PfamPF00070. Pyr_redox. 1 hit.
PF07992. Pyr_redox_2. 1 hit.
PF02852. Pyr_redox_dim. 1 hit.
[Graphical view]
PRINTSPR00368. FADPNR.
SUPFAMSSF55424. FAD/NAD-linked_reductase_dimer. 1 hit.
ProtoNetSearch...

Entry information

Entry nameSTHA_ECOHS
AccessionPrimary (citable) accession number: A8A770
Entry history
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: October 23, 2007
Last modified: January 25, 2012
This is version 35 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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