A8A671 (A8A671_ECOHS) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
tRNA (cytidine(34)-2'-O)-methyltransferase HAMAP-Rule MF_01885
EC=2.1.1.207 HAMAP-Rule MF_01885

Alternative name(s):
tRNA (cytidine/uridine-2'-O-)-methyltransferase TrmL HAMAP-Rule MF_01885

Gene names

Name:	trmL HAMAP-Rule MF_01885
Ordered Locus Names:	EcHS_A3818

Organism

Escherichia coli O9:H4 (strain HS) [Complete proteome] [HAMAP] EMBL ABV08025.1

Taxonomic identifier

331112 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	157 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Methylates the ribose at the nucleotide 34 wobble position in the two leucyl isoacceptors tRNA(Leu)(CmAA) and tRNA(Leu)(cmnm5UmAA). Catalyzes the methyl transfer from S-adenosyl-L-methionine to the 2'-OH of the wobble nucleotide By similarity. HAMAP-Rule MF_01885
Catalytic activity	S-adenosyl-L-methionine + 5-carboxymethylaminomethyluridine34 in tRNA(Leu) = S-adenosyl-L-homocysteine + 5-carboxymethylaminomethyl-2'-O-methyluridine34 in tRNA(Leu). HAMAP-Rule MF_01885 S-adenosyl-L-methionine + cytidine34 in tRNA = S-adenosyl-L-homocysteine + 2'-O-methylcytidine34 in tRNA. HAMAP-Rule MF_01885
Subunit structure	Homodimer By similarity. HAMAP-Rule MF_01885
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_01885.
Sequence similarities	Belongs to the RNA methyltransferase TrmH family. TrmL subfamily. HAMAP-Rule MF_01885

Ontologies

Keywords
Biological process	tRNA processing HAMAP-Rule MF_01885
Cellular component	Cytoplasm HAMAP-Rule MF_01885
Ligand	S-adenosyl-L-methionine HAMAP-Rule MF_01885
Molecular function	Methyltransferase HAMAP-Rule MF_01885 EMBL ABV08025.1 Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	RNA binding Inferred from electronic annotation. Source: InterPro S-adenosylmethionine-dependent methyltransferase activity Inferred from electronic annotation. Source: HAMAP tRNA methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Binding site	78	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity HAMAP-Rule MF_01885
	Binding site	100	1	S-adenosyl-L-methionine; via amide nitrogen By similarity HAMAP-Rule MF_01885
	Binding site	122	1	S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygen By similarity HAMAP-Rule MF_01885
	Binding site	130	1	S-adenosyl-L-methionine By similarity HAMAP-Rule MF_01885

Sequences

	Sequence		Length	Mass (Da)
	A8A671 [UniParc]. Last modified October 23, 2007. Version 1. Checksum: ACBE66BFF3FC2AF4	FASTA	157	17,724
	10 20 30 40 50 60 MLNIVLYEPE IPPNTGNIIR LCANTGFRLH IIEPMGFAWD DKRLRRAGLD YHEFTAVTRH 70 80 90 100 110 120 HDFRAFLEAE NPQRLFALTT KGTPAHSAVS YQDGDYLMFG PETRGLPASI LDALPAEQKI 130 140 150 RIPMVPDSRS MNLSNAVSVV VYEAWRQLGY PGALLRD

References

[1]

"The pangenome structure of Escherichia coli: comparative genomic analysis of E. coli commensal and pathogenic isolates." Rasko D.A., Rosovitz M.J., Myers G.S.A., Mongodin E.F., Fricke W.F., Gajer P., Crabtree J., Sebaihia M., Thomson N.R., Chaudhuri R., Henderson I.R., Sperandio V., Ravel J. J. Bacteriol. 190:6881-6893(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: HS.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000802 Genomic DNA. Translation: ABV08025.1.
RefSeq	YP_001460408.1. NC_009800.1.
3D structure databases
ProteinModelPortal	A8A671.
SMR	A8A671. Positions 1-154.
ModBase	Search...
Protein-protein interaction databases
STRING	331112.EcHS_A3818.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABV08025; ABV08025; EcHS_A3818.
GeneID	5592945.
KEGG	ecx:EcHS_A3818.
PATRIC	18317605. VBIEscCol77814_3729.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0219.
HOGENOM	HOG000272757.
KO	K03216.
OMA	EAWRQNG.
ProtClustDB	PRK10358.
Enzyme and pathway databases
BioCyc	ECOL331112:GHHI-3853-MONOMER.
Family and domain databases
HAMAP	MF_01885. tRNA_methyltr_TrmL.
InterPro	IPR001537. SpoU_MeTrfase. IPR016914. tRNA_cyt/urid_MeTfrase. [Graphical view]
Pfam	PF00588. SpoU_methylase. 1 hit. [Graphical view]
PIRSF	PIRSF029256. SpoU_TrmH_prd. 1 hit.
TIGRFAMs	TIGR00185. tRNA_yibK_trmL. 1 hit.
ProtoNet	Search...

Entry information

Entry name

A8A671_ECOHS

Accession

Primary (citable) accession number: A8A671

Entry history

Integrated into UniProtKB/TrEMBL:	October 23, 2007
Last sequence update:	October 23, 2007
Last modified:	May 1, 2013
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)