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A8A671 (A8A671_ECOHS) Unreviewed, UniProtKB/TrEMBL

Last modified April 16, 2014. Version 39. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
tRNA (cytidine(34)-2'-O)-methyltransferase HAMAP-Rule MF_01885

EC=2.1.1.207 HAMAP-Rule MF_01885
Alternative name(s):
tRNA (cytidine/uridine-2'-O-)-methyltransferase TrmL HAMAP-Rule MF_01885
Gene names
Name:trmL HAMAP-Rule MF_01885
Ordered Locus Names:EcHS_A3818 EMBL ABV08025.1
OrganismEscherichia coli O9:H4 (strain HS) [Complete proteome] [HAMAP] EMBL ABV08025.1
Taxonomic identifier331112 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length157 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Methylates the ribose at the nucleotide 34 wobble position in the two leucyl isoacceptors tRNA(Leu)(CmAA) and tRNA(Leu)(cmnm5UmAA). Catalyzes the methyl transfer from S-adenosyl-L-methionine to the 2'-OH of the wobble nucleotide By similarity. HAMAP-Rule MF_01885

Catalytic activity

S-adenosyl-L-methionine + 5-carboxymethylaminomethyluridine34 in tRNA(Leu) = S-adenosyl-L-homocysteine + 5-carboxymethylaminomethyl-2'-O-methyluridine34 in tRNA(Leu). HAMAP-Rule MF_01885

S-adenosyl-L-methionine + cytidine34 in tRNA = S-adenosyl-L-homocysteine + 2'-O-methylcytidine34 in tRNA. HAMAP-Rule MF_01885

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01885

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_01885.

Sequence similarities

Belongs to the RNA methyltransferase TrmH family. TrmL subfamily. HAMAP-Rule MF_01885

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Sites

Binding site781S-adenosyl-L-methionine; via carbonyl oxygen By similarity HAMAP-Rule MF_01885 PIRSR PIRSR029256-1
Binding site1001S-adenosyl-L-methionine; via amide nitrogen By similarity HAMAP-Rule MF_01885 PIRSR PIRSR029256-1
Binding site1221S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygen By similarity HAMAP-Rule MF_01885 PIRSR PIRSR029256-1
Binding site1301S-adenosyl-L-methionine By similarity HAMAP-Rule MF_01885 PIRSR PIRSR029256-1

Sequences

Sequence LengthMass (Da)Tools
A8A671 [UniParc].

Last modified October 23, 2007. Version 1.
Checksum: ACBE66BFF3FC2AF4

FASTA15717,724
        10         20         30         40         50         60 
MLNIVLYEPE IPPNTGNIIR LCANTGFRLH IIEPMGFAWD DKRLRRAGLD YHEFTAVTRH 

        70         80         90        100        110        120 
HDFRAFLEAE NPQRLFALTT KGTPAHSAVS YQDGDYLMFG PETRGLPASI LDALPAEQKI 

       130        140        150 
RIPMVPDSRS MNLSNAVSVV VYEAWRQLGY PGALLRD 

« Hide

References

[1]"The pangenome structure of Escherichia coli: comparative genomic analysis of E. coli commensal and pathogenic isolates."
Rasko D.A., Rosovitz M.J., Myers G.S., Mongodin E.F., Fricke W.F., Gajer P., Crabtree J., Sebaihia M., Thomson N.R., Chaudhuri R., Henderson I.R., Sperandio V., Ravel J.
J. Bacteriol. 190:6881-6893(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: HS EMBL ABV08025.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000802 Genomic DNA. Translation: ABV08025.1.
RefSeqYP_001460408.1. NC_009800.1.

3D structure databases

ProteinModelPortalA8A671.
SMRA8A671. Positions 1-154.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING331112.EcHS_A3818.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABV08025; ABV08025; EcHS_A3818.
GeneID5592945.
KEGGecx:EcHS_A3818.
PATRIC18317605. VBIEscCol77814_3729.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0219.
HOGENOMHOG000272757.
KOK03216.
OMAFHIVLVE.
OrthoDBEOG6RG038.
ProtClustDBPRK10358.

Enzyme and pathway databases

BioCycECOL331112:GHHI-3813-MONOMER.

Family and domain databases

HAMAPMF_01885. tRNA_methyltr_TrmL.
InterProIPR001537. SpoU_MeTrfase.
IPR016914. tRNA_cyt/urid_MeTfrase.
[Graphical view]
PfamPF00588. SpoU_methylase. 1 hit.
[Graphical view]
PIRSFPIRSF029256. SpoU_TrmH_prd. 1 hit.
TIGRFAMsTIGR00185. tRNA_yibK_trmL. 1 hit.
ProtoNetSearch...

Entry information

Entry nameA8A671_ECOHS
AccessionPrimary (citable) accession number: A8A671
Entry history
Integrated into UniProtKB/TrEMBL: October 23, 2007
Last sequence update: October 23, 2007
Last modified: April 16, 2014
This is version 39 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)