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A8A671

- A8A671_ECOHS

UniProt

A8A671 - A8A671_ECOHS

Protein

tRNA (cytidine(34)-2'-O)-methyltransferase

Gene

trmL

Organism
Escherichia coli O9:H4 (strain HS)
Status
Unreviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 42 (01 Oct 2014)
      Sequence version 1 (23 Oct 2007)
      Previous versions | rss
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    Functioni

    Methylates the ribose at the nucleotide 34 wobble position in the two leucyl isoacceptors tRNA(Leu)(CmAA) and tRNA(Leu)(cmnm5UmAA). Catalyzes the methyl transfer from S-adenosyl-L-methionine to the 2'-OH of the wobble nucleotide.UniRule annotation

    Catalytic activityi

    S-adenosyl-L-methionine + 5-carboxymethylaminomethyluridine(34) in tRNA(Leu) = S-adenosyl-L-homocysteine + 5-carboxymethylaminomethyl-2'-O-methyluridine(34) in tRNA(Leu).UniRule annotation
    S-adenosyl-L-methionine + cytidine(34) in tRNA = S-adenosyl-L-homocysteine + 2'-O-methylcytidine(34) in tRNA.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei78 – 781S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
    Binding sitei100 – 1001S-adenosyl-L-methionine; via amide nitrogenUniRule annotation
    Binding sitei122 – 1221S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygenUniRule annotation
    Binding sitei130 – 1301S-adenosyl-L-methionineUniRule annotation

    GO - Molecular functioni

    1. RNA binding Source: InterPro
    2. S-adenosylmethionine-dependent methyltransferase activity Source: UniProtKB-HAMAP
    3. tRNA methyltransferase activity Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    MethyltransferaseUniRule annotationSAAS annotationImported, Transferase

    Keywords - Biological processi

    tRNA processingUniRule annotation

    Keywords - Ligandi

    S-adenosyl-L-methionineUniRule annotation

    Enzyme and pathway databases

    BioCyciECOL331112:GHHI-3813-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA (cytidine(34)-2'-O)-methyltransferaseUniRule annotation (EC:2.1.1.207UniRule annotation)
    Alternative name(s):
    tRNA (cytidine/uridine-2'-O-)-methyltransferase TrmLUniRule annotation
    Gene namesi
    Name:trmLUniRule annotation
    Ordered Locus Names:EcHS_A3818Imported
    OrganismiEscherichia coli O9:H4 (strain HS)Imported
    Taxonomic identifieri331112 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000001123: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    CytoplasmUniRule annotation

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi331112.EcHS_A3818.

    Structurei

    3D structure databases

    ProteinModelPortaliA8A671.
    SMRiA8A671. Positions 1-154.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the RNA methyltransferase TrmH family. TrmL subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0219.
    HOGENOMiHOG000272757.
    KOiK03216.
    OMAiTHGSTPF.
    OrthoDBiEOG6RG038.

    Family and domain databases

    Gene3Di3.40.1280.10. 1 hit.
    HAMAPiMF_01885. tRNA_methyltr_TrmL.
    InterProiIPR029028. Alpha/beta_knot_MTases.
    IPR001537. SpoU_MeTrfase.
    IPR016914. tRNA_cyt/urid_MeTfrase.
    IPR029026. tRNA_m1G_MTases_N.
    [Graphical view]
    PfamiPF00588. SpoU_methylase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF029256. SpoU_TrmH_prd. 1 hit.
    SUPFAMiSSF75217. SSF75217. 1 hit.
    TIGRFAMsiTIGR00185. tRNA_yibK_trmL. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    A8A671-1 [UniParc]FASTAAdd to Basket

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    MLNIVLYEPE IPPNTGNIIR LCANTGFRLH IIEPMGFAWD DKRLRRAGLD    50
    YHEFTAVTRH HDFRAFLEAE NPQRLFALTT KGTPAHSAVS YQDGDYLMFG 100
    PETRGLPASI LDALPAEQKI RIPMVPDSRS MNLSNAVSVV VYEAWRQLGY 150
    PGALLRD 157
    Length:157
    Mass (Da):17,724
    Last modified:October 23, 2007 - v1
    Checksum:iACBE66BFF3FC2AF4
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000802 Genomic DNA. Translation: ABV08025.1.
    RefSeqiYP_001460408.1. NC_009800.1.

    Genome annotation databases

    EnsemblBacteriaiABV08025; ABV08025; EcHS_A3818.
    GeneIDi5592945.
    KEGGiecx:EcHS_A3818.
    PATRICi18317605. VBIEscCol77814_3729.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000802 Genomic DNA. Translation: ABV08025.1 .
    RefSeqi YP_001460408.1. NC_009800.1.

    3D structure databases

    ProteinModelPortali A8A671.
    SMRi A8A671. Positions 1-154.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 331112.EcHS_A3818.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABV08025 ; ABV08025 ; EcHS_A3818 .
    GeneIDi 5592945.
    KEGGi ecx:EcHS_A3818.
    PATRICi 18317605. VBIEscCol77814_3729.

    Phylogenomic databases

    eggNOGi COG0219.
    HOGENOMi HOG000272757.
    KOi K03216.
    OMAi THGSTPF.
    OrthoDBi EOG6RG038.

    Enzyme and pathway databases

    BioCyci ECOL331112:GHHI-3813-MONOMER.

    Family and domain databases

    Gene3Di 3.40.1280.10. 1 hit.
    HAMAPi MF_01885. tRNA_methyltr_TrmL.
    InterProi IPR029028. Alpha/beta_knot_MTases.
    IPR001537. SpoU_MeTrfase.
    IPR016914. tRNA_cyt/urid_MeTfrase.
    IPR029026. tRNA_m1G_MTases_N.
    [Graphical view ]
    Pfami PF00588. SpoU_methylase. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF029256. SpoU_TrmH_prd. 1 hit.
    SUPFAMi SSF75217. SSF75217. 1 hit.
    TIGRFAMsi TIGR00185. tRNA_yibK_trmL. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The pangenome structure of Escherichia coli: comparative genomic analysis of E. coli commensal and pathogenic isolates."
      Rasko D.A., Rosovitz M.J., Myers G.S.A., Mongodin E.F., Fricke W.F., Gajer P., Crabtree J., Sebaihia M., Thomson N.R., Chaudhuri R., Henderson I.R., Sperandio V., Ravel J.
      J. Bacteriol. 190:6881-6893(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: HSImported.

    Entry informationi

    Entry nameiA8A671_ECOHS
    AccessioniPrimary (citable) accession number: A8A671
    Entry historyi
    Integrated into UniProtKB/TrEMBL: October 23, 2007
    Last sequence update: October 23, 2007
    Last modified: October 1, 2014
    This is version 42 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteomeImported

    External Data

    Dasty 3