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A8A671

- A8A671_ECOHS

UniProt

A8A671 - A8A671_ECOHS

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Protein
tRNA (cytidine(34)-2'-O)-methyltransferase
Gene
trmL, EcHS_A3818
Organism
Escherichia coli O9:H4 (strain HS)
Status
Unreviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Methylates the ribose at the nucleotide 34 wobble position in the two leucyl isoacceptors tRNA(Leu)(CmAA) and tRNA(Leu)(cmnm5UmAA). Catalyzes the methyl transfer from S-adenosyl-L-methionine to the 2'-OH of the wobble nucleotide By similarity.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + 5-carboxymethylaminomethyluridine(34) in tRNA(Leu) = S-adenosyl-L-homocysteine + 5-carboxymethylaminomethyl-2'-O-methyluridine(34) in tRNA(Leu).UniRule annotation
S-adenosyl-L-methionine + cytidine(34) in tRNA = S-adenosyl-L-homocysteine + 2'-O-methylcytidine(34) in tRNA.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei78 – 781S-adenosyl-L-methionine; via carbonyl oxygen By similarityUniRule annotation
Binding sitei100 – 1001S-adenosyl-L-methionine; via amide nitrogen By similarityUniRule annotation
Binding sitei122 – 1221S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygen By similarityUniRule annotation
Binding sitei130 – 1301S-adenosyl-L-methionine By similarityUniRule annotation

GO - Molecular functioni

  1. RNA binding Source: InterPro
  2. S-adenosylmethionine-dependent methyltransferase activity Source: UniProtKB-HAMAP
  3. tRNA methyltransferase activity Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

MethyltransferaseUniRule annotationSAAS annotationsImported, Transferase

Keywords - Biological processi

tRNA processingUniRule annotation

Keywords - Ligandi

S-adenosyl-L-methionineUniRule annotation

Enzyme and pathway databases

BioCyciECOL331112:GHHI-3813-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (cytidine(34)-2'-O)-methyltransferaseUniRule annotation (EC:2.1.1.207UniRule annotation)
Alternative name(s):
tRNA (cytidine/uridine-2'-O-)-methyltransferase TrmL
Gene namesi
Name:trmLUniRule annotation
Ordered Locus Names:EcHS_A3818Imported
OrganismiEscherichia coli O9:H4 (strain HS)Imported
Taxonomic identifieri331112 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000001123: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi331112.EcHS_A3818.

Structurei

3D structure databases

ProteinModelPortaliA8A671.
SMRiA8A671. Positions 1-154.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0219.
HOGENOMiHOG000272757.
KOiK03216.
OMAiTHGSTPF.
OrthoDBiEOG6RG038.

Family and domain databases

Gene3Di3.40.1280.10. 1 hit.
HAMAPiMF_01885. tRNA_methyltr_TrmL.
InterProiIPR029028. Alpha/beta_knot_MTases.
IPR001537. SpoU_MeTrfase.
IPR016914. tRNA_cyt/urid_MeTfrase.
IPR029026. tRNA_m1G_MTases_N.
[Graphical view]
PfamiPF00588. SpoU_methylase. 1 hit.
[Graphical view]
PIRSFiPIRSF029256. SpoU_TrmH_prd. 1 hit.
SUPFAMiSSF75217. SSF75217. 1 hit.
TIGRFAMsiTIGR00185. tRNA_yibK_trmL. 1 hit.

Sequencei

Sequence statusi: Complete.

A8A671-1 [UniParc]FASTAAdd to Basket

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MLNIVLYEPE IPPNTGNIIR LCANTGFRLH IIEPMGFAWD DKRLRRAGLD    50
YHEFTAVTRH HDFRAFLEAE NPQRLFALTT KGTPAHSAVS YQDGDYLMFG 100
PETRGLPASI LDALPAEQKI RIPMVPDSRS MNLSNAVSVV VYEAWRQLGY 150
PGALLRD 157
Length:157
Mass (Da):17,724
Last modified:October 23, 2007 - v1
Checksum:iACBE66BFF3FC2AF4
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000802 Genomic DNA. Translation: ABV08025.1.
RefSeqiYP_001460408.1. NC_009800.1.

Genome annotation databases

EnsemblBacteriaiABV08025; ABV08025; EcHS_A3818.
GeneIDi5592945.
KEGGiecx:EcHS_A3818.
PATRICi18317605. VBIEscCol77814_3729.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000802 Genomic DNA. Translation: ABV08025.1 .
RefSeqi YP_001460408.1. NC_009800.1.

3D structure databases

ProteinModelPortali A8A671.
SMRi A8A671. Positions 1-154.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 331112.EcHS_A3818.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABV08025 ; ABV08025 ; EcHS_A3818 .
GeneIDi 5592945.
KEGGi ecx:EcHS_A3818.
PATRICi 18317605. VBIEscCol77814_3729.

Phylogenomic databases

eggNOGi COG0219.
HOGENOMi HOG000272757.
KOi K03216.
OMAi THGSTPF.
OrthoDBi EOG6RG038.

Enzyme and pathway databases

BioCyci ECOL331112:GHHI-3813-MONOMER.

Family and domain databases

Gene3Di 3.40.1280.10. 1 hit.
HAMAPi MF_01885. tRNA_methyltr_TrmL.
InterProi IPR029028. Alpha/beta_knot_MTases.
IPR001537. SpoU_MeTrfase.
IPR016914. tRNA_cyt/urid_MeTfrase.
IPR029026. tRNA_m1G_MTases_N.
[Graphical view ]
Pfami PF00588. SpoU_methylase. 1 hit.
[Graphical view ]
PIRSFi PIRSF029256. SpoU_TrmH_prd. 1 hit.
SUPFAMi SSF75217. SSF75217. 1 hit.
TIGRFAMsi TIGR00185. tRNA_yibK_trmL. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "The pangenome structure of Escherichia coli: comparative genomic analysis of E. coli commensal and pathogenic isolates."
    Rasko D.A., Rosovitz M.J., Myers G.S., Mongodin E.F., Fricke W.F., Gajer P., Crabtree J., Sebaihia M., Thomson N.R., Chaudhuri R., Henderson I.R., Sperandio V., Ravel J.
    J. Bacteriol. 190:6881-6893(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: HSImported.

Entry informationi

Entry nameiA8A671_ECOHS
AccessioniPrimary (citable) accession number: A8A671
Entry historyi
Integrated into UniProtKB/TrEMBL: October 23, 2007
Last sequence update: October 23, 2007
Last modified: June 11, 2014
This is version 41 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome

External Data

Dasty 3

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