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A8A5M0

- BIOH_ECOHS

UniProt

A8A5M0 - BIOH_ECOHS

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Protein

Pimeloyl-[acyl-carrier protein] methyl ester esterase

Gene
bioH, EcHS_A3609
Organism
Escherichia coli O9:H4 (strain HS)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

The physiological role of BioH is to remove the methyl group introduced by BioC when the pimeloyl moiety is complete. It allows to synthesize pimeloyl-ACP via the fatty acid synthetic pathway through the hydrolysis of the ester bonds of pimeloyl-ACP esters By similarity.UniRule annotation

Catalytic activityi

Pimeloyl-[acyl-carrier protein] methyl ester + H2O = pimeloyl-[acyl-carrier protein] + methanol.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei22 – 221Substrate; via amide nitrogen and carbonyl oxygen By similarity
Active sitei82 – 821Nucleophile By similarity
Active sitei207 – 2071 By similarity
Active sitei235 – 2351 By similarity
Binding sitei235 – 2351Substrate By similarity

GO - Molecular functioni

  1. carboxylic ester hydrolase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. biotin biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Serine esterase

Keywords - Biological processi

Biotin biosynthesis

Enzyme and pathway databases

BioCyciECOL331112:GHHI-3605-MONOMER.
UniPathwayiUPA00078.

Names & Taxonomyi

Protein namesi
Recommended name:
Pimeloyl-[acyl-carrier protein] methyl ester esterase (EC:3.1.1.85)
Alternative name(s):
Biotin synthesis protein BioH
Carboxylesterase BioH
Gene namesi
Name:bioH
Ordered Locus Names:EcHS_A3609
OrganismiEscherichia coli O9:H4 (strain HS)
Taxonomic identifieri331112 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000001123: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 256256Pimeloyl-[acyl-carrier protein] methyl ester esteraseUniRule annotationPRO_1000067265Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi331112.EcHS_A3609.

Structurei

3D structure databases

ProteinModelPortaliA8A5M0.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni82 – 832Substrate binding By similarity
Regioni143 – 1475Substrate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0596.
HOGENOMiHOG000028062.
KOiK02170.
OMAiLICELIS.
OrthoDBiEOG6FJNH9.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
HAMAPiMF_01260. Carboxylester.
InterProiIPR029058. AB_hydrolase.
IPR010076. BioH.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.
TIGRFAMsiTIGR01738. bioH. 1 hit.

Sequencei

Sequence statusi: Complete.

A8A5M0-1 [UniParc]FASTAAdd to Basket

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MNNIWWQTKG QGNVHLVLLH GWGLNAEVWR CIDEELSSHF TLHLVDLPGF    50
GRSRGFGALS LADMAEAVLQ QAPDKAIWLG WSLGGLVASQ IALTHPERVQ 100
ALVTVASSPC FSARDEWPGI KPDVLAGFQQ QLSDDFQRTV ERFLALQTMG 150
TETARQDARA LKKTVLALPM PEVDVLNGGL EILKTVDLRQ PLQNVSMPFL 200
RLYGYLDGLV PRKVVPMLDK LWPHSESYIF AKAAHAPFIS HPVEFCHLLV 250
ALKQRV 256
Length:256
Mass (Da):28,533
Last modified:October 23, 2007 - v1
Checksum:i931226F241A940F3
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000802 Genomic DNA. Translation: ABV07824.1.
RefSeqiYP_001460207.1. NC_009800.1.

Genome annotation databases

EnsemblBacteriaiABV07824; ABV07824; EcHS_A3609.
GeneIDi5594704.
KEGGiecx:EcHS_A3609.
PATRICi18317174. VBIEscCol77814_3515.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000802 Genomic DNA. Translation: ABV07824.1 .
RefSeqi YP_001460207.1. NC_009800.1.

3D structure databases

ProteinModelPortali A8A5M0.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 331112.EcHS_A3609.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABV07824 ; ABV07824 ; EcHS_A3609 .
GeneIDi 5594704.
KEGGi ecx:EcHS_A3609.
PATRICi 18317174. VBIEscCol77814_3515.

Phylogenomic databases

eggNOGi COG0596.
HOGENOMi HOG000028062.
KOi K02170.
OMAi LICELIS.
OrthoDBi EOG6FJNH9.

Enzyme and pathway databases

UniPathwayi UPA00078 .
BioCyci ECOL331112:GHHI-3605-MONOMER.

Family and domain databases

Gene3Di 3.40.50.1820. 1 hit.
HAMAPi MF_01260. Carboxylester.
InterProi IPR029058. AB_hydrolase.
IPR010076. BioH.
[Graphical view ]
SUPFAMi SSF53474. SSF53474. 1 hit.
TIGRFAMsi TIGR01738. bioH. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "The pangenome structure of Escherichia coli: comparative genomic analysis of E. coli commensal and pathogenic isolates."
    Rasko D.A., Rosovitz M.J., Myers G.S.A., Mongodin E.F., Fricke W.F., Gajer P., Crabtree J., Sebaihia M., Thomson N.R., Chaudhuri R., Henderson I.R., Sperandio V., Ravel J.
    J. Bacteriol. 190:6881-6893(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: HS.

Entry informationi

Entry nameiBIOH_ECOHS
AccessioniPrimary (citable) accession number: A8A5M0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: October 23, 2007
Last modified: June 11, 2014
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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