A8A5M0 (BIOH_ECOHS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pimelyl-[acyl-carrier protein] methyl ester esterase
EC=3.1.1.85

Alternative name(s):
Biotin synthesis protein BioH
Carboxylesterase BioH

Gene names

Name:	bioH
Ordered Locus Names:	EcHS_A3609

Organism

Escherichia coli O9:H4 (strain HS) [Complete proteome] [HAMAP]

Taxonomic identifier

331112 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	256 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	The physiological role of BioH is to remove the methyl group introduced by BioC when the pimeloyl moiety is complete. It allows to synthesize pimeloyl-ACP via the fatty acid synthetic pathway through the hydrolysis of the ester bonds of pimeloyl-ACP esters By similarity. HAMAP-Rule MF_01260
Catalytic activity	Pimelyl-[acyl-carrier protein] methyl ester + H2O = pimelyl-[acyl-carrier protein] + methanol. HAMAP-Rule MF_01260
Pathway	Cofactor biosynthesis; biotin biosynthesis. HAMAP-Rule MF_01260
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the AB hydrolase superfamily. Carboxylesterase BioH family.

Ontologies

Keywords
Biological process	Biotin biosynthesis
Cellular component	Cytoplasm
Molecular function	Hydrolase Serine esterase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	biotin biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	carboxylesterase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 256	256	Pimelyl-[acyl-carrier protein] methyl ester esterase HAMAP-Rule MF_01260		PRO_1000067265
Regions
	Region	82 – 83	2	Substrate binding By similarity
	Region	143 – 147	5	Substrate binding By similarity
Sites
	Active site	82	1	Nucleophile By similarity
	Active site	207	1	By similarity
	Active site	235	1	By similarity
	Binding site	22	1	Substrate; via amide nitrogen and carbonyl oxygen By similarity
	Binding site	235	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	A8A5M0 [UniParc]. Last modified October 23, 2007. Version 1. Checksum: 931226F241A940F3	FASTA	256	28,533
	10 20 30 40 50 60 MNNIWWQTKG QGNVHLVLLH GWGLNAEVWR CIDEELSSHF TLHLVDLPGF GRSRGFGALS 70 80 90 100 110 120 LADMAEAVLQ QAPDKAIWLG WSLGGLVASQ IALTHPERVQ ALVTVASSPC FSARDEWPGI 130 140 150 160 170 180 KPDVLAGFQQ QLSDDFQRTV ERFLALQTMG TETARQDARA LKKTVLALPM PEVDVLNGGL 190 200 210 220 230 240 EILKTVDLRQ PLQNVSMPFL RLYGYLDGLV PRKVVPMLDK LWPHSESYIF AKAAHAPFIS 250 HPVEFCHLLV ALKQRV

References

[1]

"The pangenome structure of Escherichia coli: comparative genomic analysis of E. coli commensal and pathogenic isolates." Rasko D.A., Rosovitz M.J., Myers G.S.A., Mongodin E.F., Fricke W.F., Gajer P., Crabtree J., Sebaihia M., Thomson N.R., Chaudhuri R., Henderson I.R., Sperandio V., Ravel J. J. Bacteriol. 190:6881-6893(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: HS.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000802 Genomic DNA. Translation: ABV07824.1.
RefSeq	YP_001460207.1. NC_009800.1.
3D structure databases
ProteinModelPortal	A8A5M0.
ModBase	Search...
Protein-protein interaction databases
STRING	331112.EcHS_A3609.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABV07824; ABV07824; EcHS_A3609.
GeneID	5594704.
KEGG	ecx:EcHS_A3609.
PATRIC	18317174. VBIEscCol77814_3515.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0596.
HOGENOM	HOG000028062.
KO	K02170.
OMA	LICELIS.
ProtClustDB	PRK10349.
Enzyme and pathway databases
BioCyc	ECOL331112:GHHI-3648-MONOMER.
UniPathway	UPA00078.
Family and domain databases
HAMAP	MF_01260. Carboxylester.
InterPro	IPR010076. BioH. [Graphical view]
TIGRFAMs	TIGR01738. bioH. 1 hit.
ProtoNet	Search...

Entry information

Entry name

BIOH_ECOHS

Accession

Primary (citable) accession number: A8A5M0

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	October 23, 2007
Last modified:	May 1, 2013
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program