Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

A8A5M0

- BIOH_ECOHS

UniProt

A8A5M0 - BIOH_ECOHS

Protein

Pimeloyl-[acyl-carrier protein] methyl ester esterase

Gene

bioH

Organism
Escherichia coli O9:H4 (strain HS)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 48 (01 Oct 2014)
      Sequence version 1 (23 Oct 2007)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    The physiological role of BioH is to remove the methyl group introduced by BioC when the pimeloyl moiety is complete. It allows to synthesize pimeloyl-ACP via the fatty acid synthetic pathway through the hydrolysis of the ester bonds of pimeloyl-ACP esters.UniRule annotation

    Catalytic activityi

    Pimeloyl-[acyl-carrier protein] methyl ester + H2O = pimeloyl-[acyl-carrier protein] + methanol.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei22 – 221Substrate; via amide nitrogen and carbonyl oxygenUniRule annotation
    Active sitei82 – 821NucleophileUniRule annotation
    Active sitei207 – 2071UniRule annotation
    Active sitei235 – 2351UniRule annotation
    Binding sitei235 – 2351SubstrateUniRule annotation

    GO - Molecular functioni

    1. carboxylic ester hydrolase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. biotin biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Hydrolase, Serine esterase

    Keywords - Biological processi

    Biotin biosynthesis

    Enzyme and pathway databases

    BioCyciECOL331112:GHHI-3605-MONOMER.
    UniPathwayiUPA00078.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pimeloyl-[acyl-carrier protein] methyl ester esteraseUniRule annotation (EC:3.1.1.85UniRule annotation)
    Alternative name(s):
    Biotin synthesis protein BioHUniRule annotation
    Carboxylesterase BioHUniRule annotation
    Gene namesi
    Name:bioHUniRule annotation
    Ordered Locus Names:EcHS_A3609
    OrganismiEscherichia coli O9:H4 (strain HS)
    Taxonomic identifieri331112 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000001123: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 256256Pimeloyl-[acyl-carrier protein] methyl ester esterasePRO_1000067265Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi331112.EcHS_A3609.

    Structurei

    3D structure databases

    ProteinModelPortaliA8A5M0.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni82 – 832Substrate bindingUniRule annotation
    Regioni143 – 1475Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the AB hydrolase superfamily. Carboxylesterase BioH family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0596.
    HOGENOMiHOG000028062.
    KOiK02170.
    OMAiLICELIS.
    OrthoDBiEOG6FJNH9.

    Family and domain databases

    Gene3Di3.40.50.1820. 1 hit.
    HAMAPiMF_01260. Carboxylester.
    InterProiIPR029058. AB_hydrolase.
    IPR010076. BioH.
    [Graphical view]
    SUPFAMiSSF53474. SSF53474. 1 hit.
    TIGRFAMsiTIGR01738. bioH. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    A8A5M0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNNIWWQTKG QGNVHLVLLH GWGLNAEVWR CIDEELSSHF TLHLVDLPGF    50
    GRSRGFGALS LADMAEAVLQ QAPDKAIWLG WSLGGLVASQ IALTHPERVQ 100
    ALVTVASSPC FSARDEWPGI KPDVLAGFQQ QLSDDFQRTV ERFLALQTMG 150
    TETARQDARA LKKTVLALPM PEVDVLNGGL EILKTVDLRQ PLQNVSMPFL 200
    RLYGYLDGLV PRKVVPMLDK LWPHSESYIF AKAAHAPFIS HPVEFCHLLV 250
    ALKQRV 256
    Length:256
    Mass (Da):28,533
    Last modified:October 23, 2007 - v1
    Checksum:i931226F241A940F3
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000802 Genomic DNA. Translation: ABV07824.1.
    RefSeqiYP_001460207.1. NC_009800.1.

    Genome annotation databases

    EnsemblBacteriaiABV07824; ABV07824; EcHS_A3609.
    GeneIDi5594704.
    KEGGiecx:EcHS_A3609.
    PATRICi18317174. VBIEscCol77814_3515.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000802 Genomic DNA. Translation: ABV07824.1 .
    RefSeqi YP_001460207.1. NC_009800.1.

    3D structure databases

    ProteinModelPortali A8A5M0.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 331112.EcHS_A3609.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABV07824 ; ABV07824 ; EcHS_A3609 .
    GeneIDi 5594704.
    KEGGi ecx:EcHS_A3609.
    PATRICi 18317174. VBIEscCol77814_3515.

    Phylogenomic databases

    eggNOGi COG0596.
    HOGENOMi HOG000028062.
    KOi K02170.
    OMAi LICELIS.
    OrthoDBi EOG6FJNH9.

    Enzyme and pathway databases

    UniPathwayi UPA00078 .
    BioCyci ECOL331112:GHHI-3605-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.1820. 1 hit.
    HAMAPi MF_01260. Carboxylester.
    InterProi IPR029058. AB_hydrolase.
    IPR010076. BioH.
    [Graphical view ]
    SUPFAMi SSF53474. SSF53474. 1 hit.
    TIGRFAMsi TIGR01738. bioH. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The pangenome structure of Escherichia coli: comparative genomic analysis of E. coli commensal and pathogenic isolates."
      Rasko D.A., Rosovitz M.J., Myers G.S.A., Mongodin E.F., Fricke W.F., Gajer P., Crabtree J., Sebaihia M., Thomson N.R., Chaudhuri R., Henderson I.R., Sperandio V., Ravel J.
      J. Bacteriol. 190:6881-6893(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: HS.

    Entry informationi

    Entry nameiBIOH_ECOHS
    AccessioniPrimary (citable) accession number: A8A5M0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 5, 2008
    Last sequence update: October 23, 2007
    Last modified: October 1, 2014
    This is version 48 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3