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Protein

Ribosomal RNA small subunit methyltransferase B

Gene

rsmB

Organism
Escherichia coli O9:H4 (strain HS)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Specifically methylates the cytosine at position 967 (m5C967) of 16S rRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + cytosine(967) in 16S rRNA = S-adenosyl-L-homocysteine + 5-methylcytosine(967) in 16S rRNA.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei277S-adenosyl-L-methionineUniRule annotation1
Binding sitei303S-adenosyl-L-methionineUniRule annotation1
Binding sitei322S-adenosyl-L-methionineUniRule annotation1
Active sitei375NucleophileUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, RNA-binding, Transferase
Biological processrRNA processing
LigandS-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosomal RNA small subunit methyltransferase BUniRule annotation (EC:2.1.1.176UniRule annotation)
Alternative name(s):
16S rRNA m5C967 methyltransferaseUniRule annotation
rRNA (cytosine-C(5)-)-methyltransferase RsmBUniRule annotation
Gene namesi
Name:rsmBUniRule annotation
Synonyms:sunUniRule annotation
Ordered Locus Names:EcHS_A3482
OrganismiEscherichia coli O9:H4 (strain HS)
Taxonomic identifieri331112 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003661591 – 429Ribosomal RNA small subunit methyltransferase BAdd BLAST429

Structurei

3D structure databases

ProteinModelPortaliA8A593
SMRiA8A593
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni254 – 260S-adenosyl-L-methionine bindingUniRule annotation7

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000037300
KOiK03500
OMAiLRVNRQH

Family and domain databases

Gene3Di1.10.940.101 hit
HAMAPiMF_01856 16SrRNA_methyltr_B, 1 hit
InterProiView protein in InterPro
IPR018314 Fmu/NOL1/Nop2p_CS
IPR001678 MeTrfase_RsmB/NOP2
IPR035926 NusB-like_sf
IPR006027 NusB_RsmB_TIM44
IPR023267 RCMT
IPR004573 rRNA_ssu_MeTfrase_B
IPR023541 rRNA_ssu_MeTfrase_B_ent
IPR029063 SAM-dependent_MTases
PfamiView protein in Pfam
PF01189 Methyltr_RsmB-F, 1 hit
PF01029 NusB, 1 hit
PRINTSiPR02008 RCMTFAMILY
SUPFAMiSSF48013 SSF48013, 1 hit
SSF53335 SSF53335, 1 hit
TIGRFAMsiTIGR00563 rsmB, 1 hit
PROSITEiView protein in PROSITE
PS01153 NOL1_NOP2_SUN, 1 hit
PS51686 SAM_MT_RSMB_NOP, 1 hit

Sequencei

Sequence statusi: Complete.

A8A593-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKQRNLRSM AAQAVEQVVE QGQSLSNILP PLQQKVSDKD KALLQELCFG
60 70 80 90 100
VLRTLSQLDW LINKLMARPM TGKQRTVHYL IMVGLYQLLY TRIPPHAALA
110 120 130 140 150
ETVEGAIAIK RPQLKGLING VLRQFQRQQE ELLAEFNASD ARYLHPSWLL
160 170 180 190 200
KRLQKAYPEQ WQSIVEANNQ RPPMWLRINR THHSRDSWLA LLDEAGMKGF
210 220 230 240 250
PHADYPDAVR LETPAPVHAL PGFEDGWVTV QDASAQGCMT WLAPQNGEHI
260 270 280 290 300
LDLCAAPGGK TTHILEVAPE AQVVAVDIDE QRLSRVYDNL KRLGMKATVK
310 320 330 340 350
QGDGRYPSQW CGEQQFDRIL LDAPCSATGV IRRHPDIKWL RRDRDIPELA
360 370 380 390 400
QLQSEILDAI WPHLKTGGTL VYATCSVLPE ENSLQIKAFL QRTADAELCE
410 420
TGTPEQPGKQ NLPGAEEGDG FFYAKLIKK
Length:429
Mass (Da):48,348
Last modified:October 23, 2007 - v1
Checksum:i1F516223C84D80F8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000802 Genomic DNA Translation: ABV07697.1
RefSeqiWP_000744778.1, NC_009800.1

Genome annotation databases

EnsemblBacteriaiABV07697; ABV07697; EcHS_A3482
KEGGiecx:EcHS_A3482

Similar proteinsi

Entry informationi

Entry nameiRSMB_ECOHS
AccessioniPrimary (citable) accession number: A8A593
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 3, 2009
Last sequence update: October 23, 2007
Last modified: October 25, 2017
This is version 66 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program