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A8A4A3

- TRMB_ECOHS

UniProt

A8A4A3 - TRMB_ECOHS

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Protein
tRNA (guanine-N(7)-)-methyltransferase
Gene
trmB, EcHS_A3121
Organism
Escherichia coli O9:H4 (strain HS)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei69 – 691S-adenosyl-L-methionine By similarity
Binding sitei94 – 941S-adenosyl-L-methionine By similarity
Binding sitei121 – 1211S-adenosyl-L-methionine By similarity
Active sitei144 – 1441 By similarity
Binding sitei144 – 1441S-adenosyl-L-methionine By similarity
Binding sitei148 – 1481Substrate By similarity
Binding sitei180 – 1801Substrate By similarity

GO - Molecular functioni

  1. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciECOL331112:GHHI-3112-MONOMER.
UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferase (EC:2.1.1.33)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene namesi
Name:trmB
Ordered Locus Names:EcHS_A3121
OrganismiEscherichia coli O9:H4 (strain HS)
Taxonomic identifieri331112 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000001123: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 239239tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation
PRO_1000064392Add
BLAST

Proteomic databases

PRIDEiA8A4A3.

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi331112.EcHS_A3121.

Structurei

3D structure databases

ProteinModelPortaliA8A4A3.
SMRiA8A4A3. Positions 36-239.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni150 – 1556Interaction with RNA Reviewed prediction
Regioni217 – 2204Substrate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0220.
HOGENOMiHOG000073968.
KOiK03439.
OMAiTSATNDY.
OrthoDBiEOG6K6VBC.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB.
InterProiIPR029063. SAM-dependent_MTases-like.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A8A4A3-1 [UniParc]FASTAAdd to Basket

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MKNDVISPEF DENGRPLRRI RSFVRRQGRL TKGQEHALEN YWPVMGVEFS    50
EDMLDFPALF GREAPVTLEI GFGMGASLVA MAKDRPEQDF LGIEVHSPGV 100
GACLASAHEE GLSNLRVMCH DAVEVLHKMI PDNSLRMVQL FFPDPWHKAR 150
HNKRRIVQVP FAELVKSKLQ LGGVFHMATD WEPYAEHMLE VMSSIDGYKN 200
LSESNDYVPR PASRPVTKFE QRGHRLGHGV WDLMFERVK 239
Length:239
Mass (Da):27,307
Last modified:October 23, 2007 - v1
Checksum:iB4C8AF9A1EF85FEB
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000802 Genomic DNA. Translation: ABV07357.1.
RefSeqiYP_001459740.1. NC_009800.1.

Genome annotation databases

EnsemblBacteriaiABV07357; ABV07357; EcHS_A3121.
GeneIDi5593177.
KEGGiecx:EcHS_A3121.
PATRICi18316223. VBIEscCol77814_3051.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000802 Genomic DNA. Translation: ABV07357.1 .
RefSeqi YP_001459740.1. NC_009800.1.

3D structure databases

ProteinModelPortali A8A4A3.
SMRi A8A4A3. Positions 36-239.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 331112.EcHS_A3121.

Proteomic databases

PRIDEi A8A4A3.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABV07357 ; ABV07357 ; EcHS_A3121 .
GeneIDi 5593177.
KEGGi ecx:EcHS_A3121.
PATRICi 18316223. VBIEscCol77814_3051.

Phylogenomic databases

eggNOGi COG0220.
HOGENOMi HOG000073968.
KOi K03439.
OMAi TSATNDY.
OrthoDBi EOG6K6VBC.

Enzyme and pathway databases

UniPathwayi UPA00989 .
BioCyci ECOL331112:GHHI-3112-MONOMER.

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
HAMAPi MF_01057. tRNA_methyltr_TrmB.
InterProi IPR029063. SAM-dependent_MTases-like.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view ]
Pfami PF02390. Methyltransf_4. 1 hit.
[Graphical view ]
SUPFAMi SSF53335. SSF53335. 1 hit.
TIGRFAMsi TIGR00091. TIGR00091. 1 hit.
PROSITEi PS51625. SAM_MT_TRMB. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The pangenome structure of Escherichia coli: comparative genomic analysis of E. coli commensal and pathogenic isolates."
    Rasko D.A., Rosovitz M.J., Myers G.S.A., Mongodin E.F., Fricke W.F., Gajer P., Crabtree J., Sebaihia M., Thomson N.R., Chaudhuri R., Henderson I.R., Sperandio V., Ravel J.
    J. Bacteriol. 190:6881-6893(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: HS.

Entry informationi

Entry nameiTRMB_ECOHS
AccessioniPrimary (citable) accession number: A8A4A3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: October 23, 2007
Last modified: June 11, 2014
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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