Skip Header

Contribute Send feedback
Read comments (?) or add your own

A8A3W0 (TYSY_ECOHS) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 30. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Thymidylate synthase
Short name=TS
Short name=TSase
EC=2.1.1.45
Gene names
Name:thyA
Ordered Locus Names:EcHS_A2973
OrganismEscherichia coli O9:H4 (strain HS) [Complete proteome] [HAMAP]
Taxonomic identifier331112 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length264 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Provides the sole de novo source of dTMP for DNA biosynthesis By similarity. HAMAP MF_00008

Catalytic activity

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP. HAMAP MF_00008

Pathway

Pyrimidine metabolism; dTTP biosynthesis. HAMAP MF_00008

Subunit structure

Homodimer By similarity. HAMAP MF_00008

Subcellular location

Cytoplasm By similarity HAMAP MF_00008.

Sequence similarities

Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.

Ontologies

Keywords
   Biological processNucleotide biosynthesis
   Cellular componentCytoplasm
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processdTMP biosynthetic process

Inferred from electronic annotation. Source: InterPro

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionthymidylate synthase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 264264Thymidylate synthase HAMAP MF_00008
PRO_1000057060

Sites

Active site1461 By similarity

Sequences

Sequence LengthMass (Da)Tools
A8A3W0 [UniParc].

Last modified October 23, 2007. Version 1.
Checksum: 89AF804A5ADAE96E

FASTA26430,479
        10         20         30         40         50         60 
MKQYLELMQK VLDEGTQKND RTGTGTLSIF GHQMRFNLQD GFPLVTTKRC HLRSIIHELL 

        70         80         90        100        110        120 
WFLQGNTNIA YLHENNVTIW DEWADENGDL GPVYGKQWRA WPTPDGRHID QITTVLNQLK 

       130        140        150        160        170        180 
NDPDSRRIIV SAWNVGELDK MALAPCHAFF QFYVADGKLS CQLYQRSCDV FLGLPFNIAS 

       190        200        210        220        230        240 
YALLVHMMAQ QCDLEVGDFV WTGGDTHLYS NHMDQTHLQL SREPRPLPKL IIKRKPESIF 

       250        260 
DYRFEDFEIE GYDPHPGIKA PVAI

« Hide

References

[1]"The pangenome structure of Escherichia coli: comparative genomic analysis of E. coli commensal and pathogenic isolates."
Rasko D.A., Rosovitz M.J., Myers G.S.A., Mongodin E.F., Fricke W.F., Gajer P., Crabtree J., Sebaihia M., Thomson N.R., Chaudhuri R., Henderson I.R., Sperandio V., Ravel J.
J. Bacteriol. 190:6881-6893(2008) [PubMed: 18676672] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: HS.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000802 Genomic DNA. Translation: ABV07214.1.
RefSeqYP_001459597.1. NC_009800.1.

3D structure databases

ProteinModelPortalA8A3W0.
SMRA8A3W0. Positions 2-264.
ModBaseSearch...

Protein-protein interaction databases

STRINGA8A3W0.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBESCT00000051124; EBESCP00000049109; EBESCG00000050172.
GeneID5594421.
GenomeReviewsGene locus EcHS_A2973 in contig CP000802_GR.
KEGGecx:EcHS_A2973.
PATRIC18315927. VBIEscCol77814_2904.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0207.
GeneTreeEBGT00050000009308.
HOGENOMHBG588098.
OMADIPRMKL.
ProtClustDBPRK01827.

Family and domain databases

HAMAPMF_00008. Thymidy_synth_bact.
[Tree]
InterProIPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view]
Gene3DG3DSA:3.30.572.10. Thymidylat_synth_C. 1 hit.
KOK00560.
PANTHERPTHR11549:SF2. Thymidylat_synth_C. 1 hit.
PfamPF00303. Thymidylat_synt. 1 hit.
[Graphical view]
PRINTSPR00108. THYMDSNTHASE.
SUPFAMSSF55831. Thymidylat_synth_C. 1 hit.
TIGRFAMsTIGR03284. Thym_sym. 2 hits.
PROSITEPS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTYSY_ECOHS
AccessionPrimary (citable) accession number: A8A3W0
Entry history
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: October 23, 2007
Last modified: January 25, 2012
This is version 30 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents