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Protein

Protein-L-isoaspartate O-methyltransferase

Gene

pcm

Organism
Escherichia coli O9:H4 (strain HS)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + protein L-isoaspartate = S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei59 – 591UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciECOL331112:GHHI-2866-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein-L-isoaspartate O-methyltransferaseUniRule annotation (EC:2.1.1.77UniRule annotation)
Alternative name(s):
L-isoaspartyl protein carboxyl methyltransferaseUniRule annotation
Protein L-isoaspartyl methyltransferaseUniRule annotation
Protein-beta-aspartate methyltransferaseUniRule annotation
Short name:
PIMTUniRule annotation
Gene namesi
Name:pcmUniRule annotation
Ordered Locus Names:EcHS_A2881
OrganismiEscherichia coli O9:H4 (strain HS)
Taxonomic identifieri331112 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 208208Protein-L-isoaspartate O-methyltransferasePRO_1000057599Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliA8A3M2.
SMRiA8A3M2. Positions 2-208.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the methyltransferase superfamily. L-isoaspartyl/D-aspartyl protein methyltransferase family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000257189.
KOiK00573.
OMAiHNISTRH.
OrthoDBiEOG644ZP2.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_00090. PIMT.
InterProiIPR000682. PCMT.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR11579. PTHR11579. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00080. pimt. 1 hit.
PROSITEiPS01279. PCMT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A8A3M2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVSRRVQALL DQLRAQGIQD EQVLNALAAV PREKFVDEAF EQKAWDNIAL
60 70 80 90 100
PIGQGQTISQ PYMVARMTEL LELTPQSRVL EIGTGSGYQT AILAHLVQHV
110 120 130 140 150
CSVERIKGLQ WQARRRLKNL DLHNVSTRHG DGWQGWQARA PFDAIIVTAA
160 170 180 190 200
PPEIPTALMT QLDEGGILVL PVGEEHQYLK RVRRRGGEFI IDTVEAVRFV

PLVKGELA
Length:208
Mass (Da):23,258
Last modified:October 23, 2007 - v1
Checksum:iBFA179567527A118
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000802 Genomic DNA. Translation: ABV07126.1.
RefSeqiWP_000254708.1. NC_009800.1.

Genome annotation databases

EnsemblBacteriaiABV07126; ABV07126; EcHS_A2881.
KEGGiecx:EcHS_A2881.
PATRICi18315737. VBIEscCol77814_2813.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000802 Genomic DNA. Translation: ABV07126.1.
RefSeqiWP_000254708.1. NC_009800.1.

3D structure databases

ProteinModelPortaliA8A3M2.
SMRiA8A3M2. Positions 2-208.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABV07126; ABV07126; EcHS_A2881.
KEGGiecx:EcHS_A2881.
PATRICi18315737. VBIEscCol77814_2813.

Phylogenomic databases

HOGENOMiHOG000257189.
KOiK00573.
OMAiHNISTRH.
OrthoDBiEOG644ZP2.

Enzyme and pathway databases

BioCyciECOL331112:GHHI-2866-MONOMER.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_00090. PIMT.
InterProiIPR000682. PCMT.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR11579. PTHR11579. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00080. pimt. 1 hit.
PROSITEiPS01279. PCMT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The pangenome structure of Escherichia coli: comparative genomic analysis of E. coli commensal and pathogenic isolates."
    Rasko D.A., Rosovitz M.J., Myers G.S.A., Mongodin E.F., Fricke W.F., Gajer P., Crabtree J., Sebaihia M., Thomson N.R., Chaudhuri R., Henderson I.R., Sperandio V., Ravel J.
    J. Bacteriol. 190:6881-6893(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: HS.

Entry informationi

Entry nameiPIMT_ECOHS
AccessioniPrimary (citable) accession number: A8A3M2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: October 23, 2007
Last modified: May 11, 2016
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.