Reviewed,
UniProtKB/Swiss-Prot A8A345 (HCAF_ECOHS)
Last modified
June 15, 2010.
Version 22.
History...
Clusters with 100%,
90%,
50% identity |
Documents (2) |
Third-party data |
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Names and originHide
| Protein names | Recommended name: 3-phenylpropionate/cinnamic acid dioxygenase subunit beta EC=1.14.12.19 Alternative name(s): Digoxigenin subunit beta | ||||
| Gene names |
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| Organism | Escherichia coli O9:H4 (strain HS) [Complete proteome] [HAMAP] | ||||
| Taxonomic identifier | 331112 [NCBI] | ||||
| Taxonomic lineage | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia |
Protein attributesHide
| Sequence length | 172 AA. |
| Sequence status | Complete. |
| Protein existence | Inferred from homology. |
General annotation (Comments)Hide
| Function | Part of the multicomponent 3-phenylpropionate dioxygenase. Converts 3-phenylpropionic acid (PP) and cinnamic acid (CI) into 3-phenylpropionate-dihydrodiol (PP-dihydrodiol) and cinnamic acid-dihydrodiol (CI-dihydrodiol), respectively By similarity. HAMAP MF_01649 |
| Catalytic activity | 3-phenylpropanoic acid + NADH + O2 = cis-3-(2-carboxyethyl)-3,5-cyclohexadiene-1,2-diol + NAD+. HAMAP MF_01649 Cinnamic acid + H+ + NADH + O2 = cis-3-(2-carboxyethenyl)-3,5-cyclohexadiene-1,2-diol + NAD+. HAMAP MF_01649 |
| Pathway | Aromatic compound metabolism; 3-phenylpropanoate degradation. HAMAP MF_01649 |
| Subunit structure | This dioxygenase system consists of four proteins: the two subunits of the hydroxylase component (hcaE and hcaF), a ferredoxin (hcaC) and a ferredoxin reductase (hcaD) By similarity. HAMAP MF_01649 |
| Sequence similarities | Belongs to the bacterial ring-hydroxylating dioxygenase beta subunit family. |
OntologiesHide
| Keywords | |
|---|---|
| Biological process | Aromatic hydrocarbons catabolism |
| Ligand | NAD |
| Molecular function | Dioxygenase Oxidoreductase |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | aromatic compound catabolic process Inferred from electronic annotation. Source: HAMAP oxidation reductionInferred from electronic annotation. Source: UniProtKB-KW |
| Molecular function | 3-phenylpropionate dioxygenase activity Inferred from electronic annotation. Source: HAMAP oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygenInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Sequence annotation (Features)Hide
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||
Molecule processing | |||||||
|---|---|---|---|---|---|---|---|
| Chain | 1 – 172 | 172 | 3-phenylpropionate/cinnamic acid dioxygenase subunit beta HAMAP MF_01649 | PRO_0000333712 | |||
SequencesHide
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ReferencesHide
| [1] | "The pangenome structure of Escherichia coli: comparative genomic analysis of E. coli commensal and pathogenic isolates." Rasko D.A., Rosovitz M.J., Myers G.S.A., Mongodin E.F., Fricke W.F., Gajer P., Crabtree J., Sebaihia M., Thomson N.R., Chaudhuri R., Henderson I.R., Sperandio V., Ravel J. J. Bacteriol. 190:6881-6893(2008) [PubMed: 18676672] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. |
Cross-referencesHide
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | CP000802 Genomic DNA. Translation: ABV06949.1. |
| RefSeq | YP_001459332.1. |
3D structure databases | |
| SMR | A8A345. Positions 8-171. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | A8A345. |
Genome annotation databases | |
| EnsemblBacteria | EBESCT00000052841; EBESCP00000050826; EBESCG00000051889. |
| GeneID | 5595229. |
| GenomeReviews | Gene locus EcHS_A2691 in contig CP000802_GR. |
| KEGG | ecx:EcHS_A2691. |
Organism-specific databases | |
| CMR | Search... |
Phylogenomic databases | |
| eggNOG | COG5517. |
| HOGENOM | HBG582498. |
| OMA | ARRTICV. |
| ProtClustDB | PRK10069. |
Family and domain databases | |
| HAMAP | MF_01649. HcaF. [Tree] |
| InterPro | IPR000391. Rng_hydr_dOase-bsu. [Graphical view] |
| Pfam | PF00866. Ring_hydroxyl_B. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry informationHide
| Entry name | HCAF_ECOHS | ||||||||
| Accession | Primary (citable) accession number: A8A345 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes) | ||||||||
Relevant documentsHide
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

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