Reviewed,
UniProtKB/Swiss-Prot A8A344 (HCAE_ECOHS)
Last modified
November 25, 2008.
Version 12.
History...
Clusters with 100%,
90%,
50% identity |
Documents (2) |
Third-party data |
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Names and origin
| Protein names | Recommended name: 3-phenylpropionate/cinnamic acid dioxygenase subunit alpha EC=1.14.12.19 Alternative name(s): Digoxigenin subunit alpha | ||||
| Gene names |
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| Organism | Escherichia coli O9:H4 (strain HS) [Complete proteome] [HAMAP] | ||||
| Taxonomic identifier | 331112 [NCBI] | ||||
| Taxonomic lineage | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia |
Protein attributes
| Sequence length | 453 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Inferred from homology. |
General annotation (Comments)
| Function | Part of the multicomponent 3-phenylpropionate dioxygenase. Converts 3-phenylpropionic acid (PP) and cinnamic acid (CI) into 3-phenylpropionate-dihydrodiol (PP-dihydrodiol) and cinnamic acid-dihydrodiol (CI-dihydrodiol), respectively By similarity. |
| Catalytic activity | 3-phenylpropanoic acid + NADH + O(2) = cis-3-(2-carboxyethyl)-3,5-cyclohexadiene-1,2-diol + NAD(+). Cinnamic acid + H(+) + NADH + O(2) = cis-3-(2-carboxyethenyl)-3,5-cyclohexadiene-1,2-diol + NAD(+). |
| Cofactor | Binds 1 iron ion By similarity. Binds 1 2Fe-2S cluster per subunit By similarity. |
| Pathway | Aromatic compound metabolism; 3-phenylpropionic acid degradation. |
| Subunit structure | This dioxygenase system consists of four proteins: the two subunits of the hydroxylase component (hcaE and hcaF), a ferredoxin (hcaC) and a ferredoxin reductase (hcaD) By similarity. |
| Sequence similarities | Belongs to the bacterial ring-hydroxylating dioxygenase alpha subunit family. Contains 1 Rieske domain. |
Ontologies
Keywords | |
|---|---|
| Biological process | Aromatic hydrocarbons catabolism |
| Ligand | 2Fe-2S Iron Iron-sulfur Metal-binding NAD |
| Molecular function | Dioxygenase Oxidoreductase |
| Technical term | Complete proteome |
Gene Ontology (GO) | |
| Biological process | aromatic compound catabolic process Inferred from electronic annotation. Source: HAMAP oxidation reductionInferred from electronic annotation. Source: InterPro |
| Molecular function | 2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: InterPro 3-phenylpropionate dioxygenase activityInferred from electronic annotation. Source: HAMAP electron carrier activityInferred from electronic annotation. Source: InterPro iron ion bindingInferred from electronic annotation. Source: HAMAP oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygenInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 453 | 453 | 3-phenylpropionate/cinnamic acid dioxygenase subunit alpha | PRO_0000333704 | |||||
Regions | |||||||||
| Domain | 44 – 142 | 99 | Rieske | ||||||
Sites | |||||||||
| Metal binding | 85 | 1 | Iron-sulfur (2Fe-2S) By similarity | ||||||
| Metal binding | 87 | 1 | Iron-sulfur (2Fe-2S); via pros nitrogen By similarity | ||||||
| Metal binding | 105 | 1 | Iron-sulfur (2Fe-2S) By similarity | ||||||
| Metal binding | 108 | 1 | Iron-sulfur (2Fe-2S); via pros nitrogen By similarity | ||||||
| Metal binding | 213 | 1 | Iron By similarity | ||||||
| Metal binding | 218 | 1 | Iron By similarity | ||||||
Sequences
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References
| [1] | "The pangenome structure of Escherichia coli: comparative genomic analysis of E. coli commensal and pathogenic isolates." Rasko D.A., Rosovitz M.J., Myers G.S.A., Mongodin E.F., Fricke W.F., Gajer P., Crabtree J., Sebaihia M., Thomson N.R., Chaudhuri R., Henderson I.R., Sperandio V., Ravel J. J. Bacteriol. 190:6881-6893(2008) [PubMed: 18676672] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. |
Cross-references
Sequence databases | |
|---|---|
| CP000802 Genomic DNA. Translation: ABV06948.1. | |
| RefSeq | YP_001459331.1. |
3D structure databases | |
| ModBase | Search... |
Genome annotation databases | |
| GeneID | 5595228. |
| GenomeReviews | Gene locus EcHS_A2690 in contig CP000802_GR. |
| KEGG | ecx:EcHS_A2690. |
Organism-specific databases | |
| CMR | Search... |
Family and domain databases | |
| HAMAP | MF_01648. [Tree] |
| InterPro | IPR005806. Rieske_reg. IPR015881. Ring-hydroxy_dOase_2Fe2S_BS. IPR015879. Ring_hydroxy_dOase_asu_C. IPR001663. Rng_hydr_dOase-A. [Graphical view] |
| Gene3D | G3DSA:2.102.10.10. Rieske_reg. 1 hit. |
| PANTHER | PTHR21266:SF2. Rng_hydr_dOase-A. 1 hit. |
| Pfam | PF00355. Rieske. 1 hit. PF00848. Ring_hydroxyl_A. 1 hit. [Graphical view] |
| PROSITE | PS51296. RIESKE. 1 hit. PS00570. RING_HYDROXYL_ALPHA. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | HCAE_ECOHS | ||||||||
| Accession | Primary (citable) accession number: A8A344 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes) | ||||||||
Relevant documents
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

Clusters with


