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Protein

2-keto-3-deoxy-L-rhamnonate aldolase

Gene

rhmA

Organism
Escherichia coli O9:H4 (strain HS)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the reversible retro-aldol cleavage of 2-keto-3-deoxy-L-rhamnonate (KDR) to pyruvate and lactaldehyde.UniRule annotation

Catalytic activityi

2-dehydro-3-deoxy-L-rhamnonate = pyruvate + (S)-lactaldehyde.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei49Proton acceptorUniRule annotation1
Sitei74Transition state stabilizerUniRule annotation1
Sitei88Increases basicity of active site HisUniRule annotation1
Binding sitei151SubstrateUniRule annotation1
Metal bindingi153MagnesiumUniRule annotation1
Binding sitei178Substrate; via amide nitrogenUniRule annotation1
Metal bindingi179MagnesiumUniRule annotation1
Binding sitei179SubstrateUniRule annotation1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Ligandi

Magnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
2-keto-3-deoxy-L-rhamnonate aldolaseUniRule annotation (EC:4.1.2.53UniRule annotation)
Short name:
KDR aldolaseUniRule annotation
Alternative name(s):
2-dehydro-3-deoxyrhamnonate aldolaseUniRule annotation
Gene namesi
Name:rhmAUniRule annotation
Ordered Locus Names:EcHS_A2387
OrganismiEscherichia coli O9:H4 (strain HS)
Taxonomic identifieri331112 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003531651 – 2672-keto-3-deoxy-L-rhamnonate aldolaseAdd BLAST267

Interactioni

Subunit structurei

Homohexamer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliA8A2B1.
SMRiA8A2B1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the HpcH/HpaI aldolase family. KDR aldolase subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000179750.
KOiK12660.
OMAiIARASQW.

Family and domain databases

Gene3Di3.20.20.60. 1 hit.
HAMAPiMF_01290. KDR_aldolase. 1 hit.
InterProiIPR005000. Aldolase/citrate-lyase_domain.
IPR023593. KDR_aldolase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
[Graphical view]
PfamiPF03328. HpcH_HpaI. 1 hit.
[Graphical view]
SUPFAMiSSF51621. SSF51621. 1 hit.

Sequencei

Sequence statusi: Complete.

A8A2B1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNALLSNPFK ERLRKGEVQI GLWLSSTTAY MAEIAATSGY DWLLIDGEHA
60 70 80 90 100
PNTIQDLYHQ LQAVAPYASQ PVIRPVEGSK PLIKQVLDIG AQTLLIPMVD
110 120 130 140 150
TAEQARQVVS ATRYPPYGER GVGASVARAA RWGRIENYMA QVNDSLCLLV
160 170 180 190 200
QVESKTALDN LDEILDVEGI DGVFIGPADL SASLGYPDNA GHPEVQRIIE
210 220 230 240 250
TSIRRIRAAG KAAGFLAVAP DMAQQCLAWG ANFVAVGVDT MLYSDALDQR
260
LAMFKSGKNG PRIKGSY
Length:267
Mass (Da):28,916
Last modified:October 23, 2007 - v1
Checksum:iF68506D8A11D23FE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000802 Genomic DNA. Translation: ABV06665.1.
RefSeqiWP_000992954.1. NC_009800.1.

Genome annotation databases

EnsemblBacteriaiABV06665; ABV06665; EcHS_A2387.
KEGGiecx:EcHS_A2387.
PATRICi18314754. VBIEscCol77814_2339.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000802 Genomic DNA. Translation: ABV06665.1.
RefSeqiWP_000992954.1. NC_009800.1.

3D structure databases

ProteinModelPortaliA8A2B1.
SMRiA8A2B1.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABV06665; ABV06665; EcHS_A2387.
KEGGiecx:EcHS_A2387.
PATRICi18314754. VBIEscCol77814_2339.

Phylogenomic databases

HOGENOMiHOG000179750.
KOiK12660.
OMAiIARASQW.

Family and domain databases

Gene3Di3.20.20.60. 1 hit.
HAMAPiMF_01290. KDR_aldolase. 1 hit.
InterProiIPR005000. Aldolase/citrate-lyase_domain.
IPR023593. KDR_aldolase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
[Graphical view]
PfamiPF03328. HpcH_HpaI. 1 hit.
[Graphical view]
SUPFAMiSSF51621. SSF51621. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRHMA_ECOHS
AccessioniPrimary (citable) accession number: A8A2B1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 4, 2008
Last sequence update: October 23, 2007
Last modified: November 2, 2016
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.