A8A1K5 (COBT_ECOHS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase
Short name=NN:DBI PRT
EC=2.4.2.21

Alternative name(s):
N(1)-alpha-phosphoribosyltransferase

Gene names

Name:	cobT
Ordered Locus Names:	EcHS_A2114

Organism

Escherichia coli O9:H4 (strain HS) [Complete proteome] [HAMAP]

Taxonomic identifier

331112 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	359 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the synthesis of alpha-ribazole-5'-phosphate from nicotinate mononucleotide (NAMN) and 5,6-dimethylbenzimidazole (DMB) By similarity. HAMAP-Rule MF_00230
Catalytic activity	Beta-nicotinate D-ribonucleotide + 5,6-dimethylbenzimidazole = nicotinate + alpha-ribazole 5'-phosphate. HAMAP-Rule MF_00230
Pathway	Nucleoside biosynthesis; alpha-ribazole biosynthesis; alpha-ribazole from 5,6-dimethylbenzimidazole: step 1/2. HAMAP-Rule MF_00230
Subunit structure	Homodimer By similarity. HAMAP-Rule MF_00230
Sequence similarities	Belongs to the CobT family.

Ontologies

Keywords
Biological process	Cobalamin biosynthesis
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	cobalamin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW nucleoside biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular_function	cobalamin-transporting ATPase activity Inferred from electronic annotation. Source: HAMAP nicotinate-nucleotide-dimethylbenzimidazole phosphoribosyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 359	359	Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase HAMAP-Rule MF_00230		PRO_1000058765
Sites
	Active site	318	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	A8A1K5 [UniParc]. Last modified October 23, 2007. Version 1. Checksum: F8F54BDA8BE2983A	FASTA	359	36,925
	10 20 30 40 50 60 MQTLADLLNT IPAIDPAAMS RAQRHIDGLL KPVGSLGRLE ALAIQLAGMP GLNGIPHVGK 70 80 90 100 110 120 KAVLVMCADH GVWEEGVAIS PKEVTAIQAE NMTRGTTGVC VLAAQAGANV HVVDVGIDSA 130 140 150 160 170 180 EPIPGLINMR VARGSGNIAS APAMSRRQAE KLLLDVICYT RELAKNGVTL FGVGELGMAN 190 200 210 220 230 240 TTPAAAIVST ITGRDPEEVV GIGANLPTDK LANKIDVVRR AITLNQPNPQ DGIDVLAKVG 250 260 270 280 290 300 GFDLVGIAGV MLGAASCGLP VLLDGFLSYA AALAACQMSP AIKPYLIPSH LSVEKGARIA 310 320 330 340 350 LSHLGLEPYL NMDMRLGEGS GAALAMPIIE AACAIYNNMG ELAASNIVLP GNTTSDLNS

References

[1]

"The pangenome structure of Escherichia coli: comparative genomic analysis of E. coli commensal and pathogenic isolates." Rasko D.A., Rosovitz M.J., Myers G.S.A., Mongodin E.F., Fricke W.F., Gajer P., Crabtree J., Sebaihia M., Thomson N.R., Chaudhuri R., Henderson I.R., Sperandio V., Ravel J. J. Bacteriol. 190:6881-6893(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: HS.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000802 Genomic DNA. Translation: ABV06409.1.
RefSeq	YP_001458792.1. NC_009800.1.
3D structure databases
ProteinModelPortal	A8A1K5.
SMR	A8A1K5. Positions 4-350.
ModBase	Search...
Protein-protein interaction databases
STRING	331112.EcHS_A2114.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABV06409; ABV06409; EcHS_A2114.
GeneID	5594700.
KEGG	ecx:EcHS_A2114.
PATRIC	18314203. VBIEscCol77814_2065.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2038.
HOGENOM	HOG000263499.
KO	K00768.
OMA	FICSVAA.
ProtClustDB	PRK00105.
Enzyme and pathway databases
BioCyc	ECOL331112:GHHI-2103-MONOMER.
UniPathway	UPA00061; UER00516.
Family and domain databases
Gene3D	1.10.1610.10. 1 hit.
HAMAP	MF_00230. CobT.
InterPro	IPR003200. Nict_dMeBzImd_PRibTrfase-like. IPR023195. Nict_dMeBzImd_PRibTrfase_N. IPR017846. Nict_dMeBzImd_PRibTrfase_pro. [Graphical view]
Pfam	PF02277. DBI_PRT. 1 hit. [Graphical view]
SUPFAM	SSF52733. NN:DBI_PRT. 1 hit.
TIGRFAMs	TIGR03160. cobT_DBIPRT. 1 hit.
ProtoNet	Search...

Entry information

Entry name

COBT_ECOHS

Accession

Primary (citable) accession number: A8A1K5

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	October 23, 2007
Last modified:	May 29, 2013
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program