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A8A1A0

- OTSA_ECOHS

UniProt

A8A1A0 - OTSA_ECOHS

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Protein

Alpha,alpha-trehalose-phosphate synthase [UDP-forming]

Gene
otsA, EcHS_A1993
Organism
Escherichia coli O9:H4 (strain HS)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the transfer of glucose from UDP-glucose to glucose-6-phosphate to form alpha,alpha-1,1 trehalose-6-phosphate. Acts with retention of the anomeric configuration of the UDP-sugar donor By similarity.

Catalytic activityi

UDP-glucose + D-glucose 6-phosphate = UDP + alpha,alpha-trehalose 6-phosphate.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei10 – 101Glucose-6-phosphate By similarity
Binding sitei77 – 771Glucose-6-phosphate By similarity
Sitei86 – 861Involved in alpha anomer selectivity By similarity
Binding sitei131 – 1311Glucose-6-phosphate By similarity
Sitei156 – 1561Involved in alpha anomer selectivity By similarity
Binding sitei263 – 2631UDP-glucose By similarity
Binding sitei268 – 2681UDP-glucose By similarity
Binding sitei301 – 3011Glucose-6-phosphate By similarity
Binding sitei340 – 3401UDP-glucose; via amide nitrogen and carbonyl oxygen By similarity

GO - Molecular functioni

  1. alpha,alpha-trehalose-phosphate synthase (UDP-forming) activity Source: UniProtKB-EC

GO - Biological processi

  1. trehalose biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

BioCyciECOL331112:GHHI-1986-MONOMER.
UniPathwayiUPA00299.

Protein family/group databases

CAZyiGT20. Glycosyltransferase Family 20.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha,alpha-trehalose-phosphate synthase [UDP-forming] (EC:2.4.1.15)
Alternative name(s):
Osmoregulatory trehalose synthesis protein A
Trehalose-6-phosphate synthase
UDP-glucose-glucosephosphate glucosyltransferase
Gene namesi
Name:otsA
Ordered Locus Names:EcHS_A1993
OrganismiEscherichia coli O9:H4 (strain HS)
Taxonomic identifieri331112 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000001123: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 474474Alpha,alpha-trehalose-phosphate synthase [UDP-forming]PRO_0000348901Add
BLAST

Interactioni

Subunit structurei

Homotetramer By similarity.

Protein-protein interaction databases

STRINGi331112.EcHS_A1993.

Structurei

3D structure databases

ProteinModelPortaliA8A1A0.
SMRiA8A1A0. Positions 2-457.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni22 – 232UDP-glucose binding By similarity
Regioni366 – 3705UDP-glucose binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0380.
HOGENOMiHOG000191478.
KOiK00697.
OMAiWGESFVK.
OrthoDBiEOG6CCH1M.

Family and domain databases

InterProiIPR001830. Glyco_trans_20.
IPR012766. Trehalose_OtsA.
[Graphical view]
PfamiPF00982. Glyco_transf_20. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02400. trehalose_OtsA. 1 hit.

Sequencei

Sequence statusi: Complete.

A8A1A0-1 [UniParc]FASTAAdd to Basket

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MSRLVVVSNR IAPPDEHAAS AGGLAVGILG ALKAAGGLWF GWSGETGNED    50
QPLKKVKKGN ITWASFNLSE QDLDEYYNQF SNAVLWPAFH YRLDLVQFQR 100
PAWDGYLRVN ALLADKLLPL LQDDDIIWIH DYHLLPFAHE LRKRGVNNRI 150
GFFLHIPFPT PEIFNALPTY DTLLEQLCDY DLLGFQTEND RLAFLDCLSN 200
LTRVTTRSAK SHTAWGKAFR TEVYPIGIEP KEIAKQAAGP LPPKLAQLKA 250
ELKNVQNIFS VERLDYSKGL PERFLAYEVL LEKYPQHHGK IRYTQIAPTS 300
RGDVQAYQDI RHQLENEAGR INGKYGQLGW TPLYYLNQHF DRKLLMKIFR 350
YSDVGLVTPL RDGMNLVAKE YVAAQDPANP GVLVLSQFAG AANELTSALI 400
VNPYDRDEVA AALDRALTMS LAERISRHAE MLDVIVKNDI NHWQECFISD 450
LKQIVPRSAE SQQRDKVATF PKLA 474
Length:474
Mass (Da):53,639
Last modified:October 23, 2007 - v1
Checksum:iBD24A47FBC18E613
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000802 Genomic DNA. Translation: ABV06304.1.
RefSeqiYP_001458687.1. NC_009800.1.

Genome annotation databases

EnsemblBacteriaiABV06304; ABV06304; EcHS_A1993.
GeneIDi5592242.
KEGGiecx:EcHS_A1993.
PATRICi18313962. VBIEscCol77814_1952.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000802 Genomic DNA. Translation: ABV06304.1 .
RefSeqi YP_001458687.1. NC_009800.1.

3D structure databases

ProteinModelPortali A8A1A0.
SMRi A8A1A0. Positions 2-457.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 331112.EcHS_A1993.

Protein family/group databases

CAZyi GT20. Glycosyltransferase Family 20.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABV06304 ; ABV06304 ; EcHS_A1993 .
GeneIDi 5592242.
KEGGi ecx:EcHS_A1993.
PATRICi 18313962. VBIEscCol77814_1952.

Phylogenomic databases

eggNOGi COG0380.
HOGENOMi HOG000191478.
KOi K00697.
OMAi WGESFVK.
OrthoDBi EOG6CCH1M.

Enzyme and pathway databases

UniPathwayi UPA00299 .
BioCyci ECOL331112:GHHI-1986-MONOMER.

Family and domain databases

InterProi IPR001830. Glyco_trans_20.
IPR012766. Trehalose_OtsA.
[Graphical view ]
Pfami PF00982. Glyco_transf_20. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR02400. trehalose_OtsA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "The pangenome structure of Escherichia coli: comparative genomic analysis of E. coli commensal and pathogenic isolates."
    Rasko D.A., Rosovitz M.J., Myers G.S.A., Mongodin E.F., Fricke W.F., Gajer P., Crabtree J., Sebaihia M., Thomson N.R., Chaudhuri R., Henderson I.R., Sperandio V., Ravel J.
    J. Bacteriol. 190:6881-6893(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: HS.

Entry informationi

Entry nameiOTSA_ECOHS
AccessioniPrimary (citable) accession number: A8A1A0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: October 23, 2007
Last modified: May 14, 2014
This is version 39 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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