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Protein

Trehalose-6-phosphate synthase

Gene

otsA

Organism
Escherichia coli O9:H4 (strain HS)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Probably involved in the osmoprotection via the biosynthesis of trehalose. Catalyzes the transfer of glucose from UDP-alpha-D-glucose (UDP-Glc) to D-glucose 6-phosphate (Glc-6-P) to form trehalose-6-phosphate. Acts with retention of the anomeric configuration of the UDP-sugar donor.By similarity

Catalytic activityi

UDP-alpha-D-glucose + D-glucose 6-phosphate = UDP + alpha,alpha-trehalose 6-phosphate.By similarity

Pathwayi: trehalose biosynthesis

This protein is involved in the pathway trehalose biosynthesis, which is part of Glycan biosynthesis.By similarity
View all proteins of this organism that are known to be involved in the pathway trehalose biosynthesis and in Glycan biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei10D-glucose 6-phosphateBy similarity1
Binding sitei77D-glucose 6-phosphateBy similarity1
Sitei86Involved in alpha anomer selectivityBy similarity1
Binding sitei131D-glucose 6-phosphateBy similarity1
Sitei156Involved in alpha anomer selectivityBy similarity1
Binding sitei263UDP-glucoseBy similarity1
Binding sitei268UDP-glucoseBy similarity1
Binding sitei301D-glucose 6-phosphateBy similarity1
Binding sitei340UDP-glucose; via amide nitrogen and carbonyl oxygenBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Transferase

Enzyme and pathway databases

BioCyciECOL331112:G1G9Q-2060-MONOMER
UniPathwayiUPA00299

Protein family/group databases

CAZyiGT20 Glycosyltransferase Family 20

Names & Taxonomyi

Protein namesi
Recommended name:
Trehalose-6-phosphate synthaseBy similarity (EC:2.4.1.15By similarity)
Short name:
TPSBy similarity
Alternative name(s):
Alpha,alpha-trehalose-phosphate synthase [UDP-forming]By similarity
Osmoregulatory trehalose synthesis protein ABy similarity
Short name:
OtsABy similarity
UDP-glucose-glucosephosphate glucosyltransferaseBy similarity
Gene namesi
Name:otsABy similarity
Ordered Locus Names:EcHS_A1993
OrganismiEscherichia coli O9:H4 (strain HS)
Taxonomic identifieri331112 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003489011 – 474Trehalose-6-phosphate synthaseAdd BLAST474

Interactioni

Subunit structurei

Homotetramer.By similarity

Structurei

3D structure databases

ProteinModelPortaliA8A1A0
SMRiA8A1A0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni22 – 23UDP-glucose bindingBy similarity2
Regioni366 – 370UDP-glucose bindingBy similarity5

Sequence similaritiesi

Belongs to the glycosyltransferase 20 family.By similarity

Phylogenomic databases

HOGENOMiHOG000191478
KOiK00697
OMAiIEFMPIH

Family and domain databases

CDDicd03788 GT1_TPS, 1 hit
InterProiView protein in InterPro
IPR001830 Glyco_trans_20
IPR012766 Trehalose_OtsA
PfamiView protein in Pfam
PF00982 Glyco_transf_20, 1 hit
TIGRFAMsiTIGR02400 trehalose_OtsA, 1 hit

Sequencei

Sequence statusi: Complete.

A8A1A0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRLVVVSNR IAPPDEHAAS AGGLAVGILG ALKAAGGLWF GWSGETGNED
60 70 80 90 100
QPLKKVKKGN ITWASFNLSE QDLDEYYNQF SNAVLWPAFH YRLDLVQFQR
110 120 130 140 150
PAWDGYLRVN ALLADKLLPL LQDDDIIWIH DYHLLPFAHE LRKRGVNNRI
160 170 180 190 200
GFFLHIPFPT PEIFNALPTY DTLLEQLCDY DLLGFQTEND RLAFLDCLSN
210 220 230 240 250
LTRVTTRSAK SHTAWGKAFR TEVYPIGIEP KEIAKQAAGP LPPKLAQLKA
260 270 280 290 300
ELKNVQNIFS VERLDYSKGL PERFLAYEVL LEKYPQHHGK IRYTQIAPTS
310 320 330 340 350
RGDVQAYQDI RHQLENEAGR INGKYGQLGW TPLYYLNQHF DRKLLMKIFR
360 370 380 390 400
YSDVGLVTPL RDGMNLVAKE YVAAQDPANP GVLVLSQFAG AANELTSALI
410 420 430 440 450
VNPYDRDEVA AALDRALTMS LAERISRHAE MLDVIVKNDI NHWQECFISD
460 470
LKQIVPRSAE SQQRDKVATF PKLA
Length:474
Mass (Da):53,639
Last modified:October 23, 2007 - v1
Checksum:iBD24A47FBC18E613
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000802 Genomic DNA Translation: ABV06304.1
RefSeqiWP_012135897.1, NC_009800.1

Genome annotation databases

EnsemblBacteriaiABV06304; ABV06304; EcHS_A1993
KEGGiecx:EcHS_A1993

Similar proteinsi

Entry informationi

Entry nameiOTSA_ECOHS
AccessioniPrimary (citable) accession number: A8A1A0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: October 23, 2007
Last modified: March 28, 2018
This is version 60 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health